##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049349_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5597736
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.81962886424083	31.0	31.0	34.0	28.0	34.0
2	31.009789493466645	31.0	31.0	34.0	28.0	34.0
3	31.07372730689693	31.0	31.0	34.0	28.0	34.0
4	34.7643650933163	37.0	35.0	37.0	32.0	37.0
5	34.77622381619998	37.0	35.0	37.0	32.0	37.0
6	34.88977293677301	37.0	35.0	37.0	32.0	37.0
7	34.8655724385716	37.0	35.0	37.0	32.0	37.0
8	34.818100746444635	37.0	35.0	37.0	32.0	37.0
9	36.45940465931226	39.0	37.0	39.0	32.0	39.0
10-11	36.41695160329104	39.0	37.0	39.0	32.0	39.0
12-13	36.35893814928035	39.0	37.0	39.0	32.0	39.0
14-15	37.593910198694616	40.0	38.0	41.0	32.0	41.0
16-17	37.55905092701764	40.0	38.0	41.0	32.0	41.0
18-19	37.58443118789454	40.0	38.0	41.0	32.0	41.0
20-21	37.65862752369887	40.0	38.0	41.0	32.0	41.0
22-23	37.61515548428865	40.0	38.0	41.0	32.0	41.0
24-25	37.62134816647301	40.0	38.0	41.0	32.0	41.0
26-27	37.60968077094026	40.0	38.0	41.0	32.0	41.0
28-29	37.55262975960281	40.0	38.0	41.0	32.0	41.0
30-31	37.491509960455446	40.0	38.0	41.0	32.0	41.0
32-33	37.44394019296372	40.0	38.0	41.0	32.0	41.0
34-35	37.34709997041661	40.0	38.0	41.0	32.0	41.0
36-37	37.276544838841986	40.0	37.0	41.0	31.0	41.0
38-39	37.17922969214697	40.0	37.0	41.0	31.0	41.0
40-41	37.08122033622165	40.0	37.0	41.0	31.0	41.0
42-43	36.95016172609783	40.0	37.0	41.0	30.5	41.0
44-45	36.83647156636183	40.0	37.0	41.0	30.0	41.0
46-47	36.72385523361588	40.0	37.0	41.0	30.0	41.0
48-49	36.59809322911977	40.0	36.0	41.0	30.0	41.0
50-51	36.45974479682501	40.0	36.0	41.0	30.0	41.0
52-53	36.31770746244553	39.5	36.0	41.0	30.0	41.0
54-55	36.17584082207521	39.0	35.0	41.0	29.5	41.0
56-57	36.05013026695078	39.0	35.0	41.0	29.0	41.0
58-59	35.842567066399695	39.0	35.0	41.0	29.0	41.0
60-61	35.6228347496202	39.0	35.0	40.5	28.0	41.0
62-63	35.41053883212784	38.5	35.0	40.0	28.0	41.0
64-65	35.161395071150196	38.0	34.5	40.0	28.0	41.0
66-67	34.893723462485546	37.5	34.0	40.0	28.0	41.0
68-69	34.599034145233006	37.0	34.0	40.0	28.0	41.0
70-71	34.30963866463156	36.5	34.0	39.5	27.0	41.0
72-73	33.959400997117406	36.0	34.0	39.0	26.5	41.0
74-75	33.60258450916585	35.5	34.0	39.0	26.0	40.5
76-77	33.261905348876766	35.0	33.0	38.0	26.0	40.0
78-79	32.93864001446299	35.0	33.0	37.0	26.0	39.0
80-81	32.572727974309615	35.0	33.0	37.0	25.5	39.0
82-83	32.25305427051222	35.0	33.0	36.0	25.0	39.0
84-85	31.949642230358847	35.0	33.0	36.0	25.0	37.0
86-87	31.625380778943487	35.0	32.5	36.0	23.5	37.0
88-89	31.36634694812331	35.0	32.0	35.0	23.0	37.0
90-91	31.15234364393033	35.0	32.0	35.0	21.5	36.0
92-93	30.90651443726535	35.0	32.0	35.0	20.0	36.0
94-95	30.702239262444674	35.0	32.0	35.0	18.5	36.0
96-97	30.447385514429406	35.0	32.0	35.0	12.0	35.5
98-99	30.233266717115633	35.0	32.0	35.0	2.0	35.0
100	30.10390540032613	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3386594966074341
1101	2	0.3807220995651157
1101	3	0.3482470555790833
1101	4	0.45014413723946234
1101	5	0.3957323458219335
1101	6	0.49936108943448687
1101	7	0.47948442440631567
1101	8	0.503671390343456
1101	9	0.5977117193628629
1101	10-11	0.6129404901669346
1101	12-13	0.6353726020719606
1101	14-15	0.7092514277566977
1101	16-17	0.7515642812880117
1101	18-19	0.7258972436386912
1101	20-21	0.7085498317158851
1101	22-23	0.733862017216353
1101	24-25	0.7670147250715615
1101	26-27	0.6661837418711158
1101	28-29	0.723775360652013
1101	30-31	0.7473434696719607
1101	32-33	0.7401913719041033
1101	34-35	0.7391594009659741
1101	36-37	0.7434000805389758
1101	38-39	0.7867381348465301
1101	40-41	0.8437051376498346
1101	42-43	0.7907633080085503
1101	44-45	0.7504068115915956
1101	46-47	0.7188052453819225
1101	48-49	0.6764606896170235
1101	50-51	0.7256222241632315
1101	52-53	0.7014604052553111
1101	54-55	0.7127458515474885
1101	56-57	0.7368882044596958
1101	58-59	0.7370575958842096
1101	60-61	0.8187263945873227
1101	62-63	0.8410868291778186
1101	64-65	0.7725732044249298
1101	66-67	0.7507382435841734
1101	68-69	0.7438022273607601
1101	70-71	0.7665248588796345
1101	72-73	0.833310981548415
1101	74-75	0.7971851443645761
1101	76-77	0.8138896151355652
1101	78-79	0.7801194264878006
1101	80-81	0.785162223376652
1101	82-83	0.7851088037183729
1101	84-85	0.7529066076144879
1101	86-87	0.7553452740919973
1101	88-89	0.8427402234502281
1101	90-91	0.8655793071759561
1101	92-93	0.8559044301240384
1101	94-95	0.8174775529727505
1101	96-97	0.8828626860544446
1101	98-99	0.9362120158662712
1101	100	0.9473320737448567
1102	1	0.14385951573326494
1102	2	0.1765193397303797
1102	3	0.1974619487163345
1102	4	0.2914631179005909
1102	5	0.21602677610275833
1102	6	0.3712904528753498
1102	7	0.4367701634683101
1102	8	0.42711968708355386
1102	9	0.5861748258900619
1102	10-11	0.4647189802278717
1102	12-13	0.5033939019098099
1102	14-15	0.6187484778236438
1102	16-17	0.6009137059711378
1102	18-19	0.5976601048403225
1102	20-21	0.4958444235477444
1102	22-23	0.5452179509063058
1102	24-25	0.6111036975306519
1102	26-27	0.5813414436818718
1102	28-29	0.560596800368728
1102	30-31	0.5488744980786251
1102	32-33	0.6071119462335659
1102	34-35	0.5929524690972272
1102	36-37	0.5431838964081521
1102	38-39	0.5796120370563855
1102	40-41	0.5705468264154163
1102	42-43	0.578190592364102
1102	44-45	0.5523855711683012
1102	46-47	0.49495225059088455
1102	48-49	0.5452811153251957
1102	50-51	0.5977087532691527
1102	52-53	0.5509128151807161
1102	54-55	0.5488688872242307
1102	56-57	0.5412977269731485
1102	58-59	0.6076761608980377
1102	60-61	0.5852906795882262
1102	62-63	0.5730592483718908
1102	64-65	0.5584598125805087
1102	66-67	0.5788125864803177
1102	68-69	0.5759408092822724
1102	70-71	0.5808519451017702
1102	72-73	0.6146355415199167
1102	74-75	0.6018828275078505
1102	76-77	0.6137682113788117
1102	78-79	0.6506259526116764
1102	80-81	0.615655587760223
1102	82-83	0.6109904663189099
1102	84-85	0.6170876047436273
1102	86-87	0.6211581912140538
1102	88-89	0.6153077841740355
1102	90-91	0.6389003011419803
1102	92-93	0.6291948680291739
1102	94-95	0.6036663266193933
1102	96-97	0.5981363594333544
1102	98-99	0.6150212744658923
1102	100	0.5832167155613348
1103	1	0.11417821256302574
1103	2	0.2241907001681156
1103	3	0.17521016224241848
1103	4	0.20007946688836142
1103	5	0.2456888168039555
1103	6	0.36724896910673266
1103	7	0.41878671916816046
1103	8	0.4011870717476782
1103	9	0.5249509334407989
1103	10-11	0.5088401779537861
1103	12-13	0.5229850738578037
1103	14-15	0.5932858635299993
1103	16-17	0.5262788998872807
1103	18-19	0.4991263550170757
1103	20-21	0.5241650246238763
1103	22-23	0.5198910625634738
1103	24-25	0.5293067195670602
1103	26-27	0.5026828631317741
1103	28-29	0.5296246635862403
1103	30-31	0.5581255430195355
1103	32-33	0.610443064409651
1103	34-35	0.5618061534799565
1103	36-37	0.589492321073962
1103	38-39	0.5723104164294526
1103	40-41	0.565258518092044
1103	42-43	0.5413331067900984
1103	44-45	0.5303448632464622
1103	46-47	0.6187237177760068
1103	48-49	0.5744016772935581
1103	50-51	0.5695392576962135
1103	52-53	0.5856660241231211
1103	54-55	0.5823230083542299
1103	56-57	0.53349752383496
1103	58-59	0.6245424700598932
1103	60-61	0.5858581618536576
1103	62-63	0.5627098023648287
1103	64-65	0.5479778910641997
1103	66-67	0.6474105139507387
1103	68-69	0.6878596853698795
1103	70-71	0.6103543859191944
1103	72-73	0.6337735490725649
1103	74-75	0.6363675307616674
1103	76-77	0.7234847852026718
1103	78-79	0.6181382293934021
1103	80-81	0.6233544107465079
1103	82-83	0.6132843413677449
1103	84-85	0.6647363561985848
1103	86-87	0.6328334090773104
1103	88-89	0.6365351611607153
1103	90-91	0.7377346607688828
1103	92-93	0.6404657101447668
1103	94-95	0.6991980054309934
1103	96-97	0.7030520470619486
1103	98-99	0.781231395530753
1103	100	0.7604411340377233
1104	1	0.06995076794088817
1104	2	0.06095964021887568
1104	3	0.05436549249911948
1104	4	0.1955472397035578
1104	5	0.20268279818791513
1104	6	0.22252902215904413
1104	7	0.28748054315022387
1104	8	0.26556501575605296
1104	9	0.37889440695592924
1104	10-11	0.338158032271032
1104	12-13	0.28679012447704366
1104	14-15	0.385449467619722
1104	16-17	0.43665748678032656
1104	18-19	0.4033005806027248
1104	20-21	0.3139615500413342
1104	22-23	0.36138576271211775
1104	24-25	0.4233110946087564
1104	26-27	0.36465389107637947
1104	28-29	0.36894151267229347
1104	30-31	0.33252788842081316
1104	32-33	0.32997588001131817
1104	34-35	0.4212761963863798
1104	36-37	0.33219908376529617
1104	38-39	0.3996453927783179
1104	40-41	0.4203643016065186
1104	42-43	0.36204939924385826
1104	44-45	0.38644709970908764
1104	46-47	0.31979613913624405
1104	48-49	0.36402280541229004
1104	50-51	0.3844442439934568
1104	52-53	0.3386779610454411
1104	54-55	0.3770872214409309
1104	56-57	0.34545330871950597
1104	58-59	0.49749307324890424
1104	60-61	0.41415100344017475
1104	62-63	0.3740157683753935
1104	64-65	0.4234306451523082
1104	66-67	0.458212104142369
1104	68-69	0.42685779874783236
1104	70-71	0.41482119078025903
1104	72-73	0.42583759778354846
1104	74-75	0.4700146076831544
1104	76-77	0.5027158827033062
1104	78-79	0.4167419806370276
1104	80-81	0.4092973543359406
1104	82-83	0.3627455840816083
1104	84-85	0.50663650162792
1104	86-87	0.463986343726031
1104	88-89	0.43088620990062054
1104	90-91	0.41501345328931905
1104	92-93	0.433953945602223
1104	94-95	0.4226540268557706
1104	96-97	0.3978268763410462
1104	98-99	0.48345877358451617
1104	100	0.4587564695772208
1105	1	7.988891209151916E-4
1105	2	0.06603265792830726
1105	3	0.07070878616013232
1105	4	0.1620190376255337
1105	5	0.15354685841461446
1105	6	0.24435867788611176
1105	7	0.26534021746338965
1105	8	0.29037178063203584
1105	9	0.34242959274423157
1105	10-11	0.32631729800692
1105	12-13	0.3149665302853677
1105	14-15	0.3376808517131167
1105	16-17	0.33996528732408393
1105	18-19	0.2846815759724919
1105	20-21	0.30635453284580905
1105	22-23	0.3580418084580117
1105	24-25	0.30464823026470356
1105	26-27	0.30163573357429385
1105	28-29	0.37711361509422403
1105	30-31	0.339737832381914
1105	32-33	0.3373453098963637
1105	34-35	0.39260963929307025
1105	36-37	0.3525330400873514
1105	38-39	0.3863427018008352
1105	40-41	0.417375404933523
1105	42-43	0.39727849765678513
1105	44-45	0.3736080272400031
1105	46-47	0.3249109121610303
1105	48-49	0.3117368342349991
1105	50-51	0.37897233791552765
1105	52-53	0.3719699932085163
1105	54-55	0.4078388107535247
1105	56-57	0.319140915438183
1105	58-59	0.32472722343307225
1105	60-61	0.3212916946125617
1105	62-63	0.2977951283137017
1105	64-65	0.3292987465472308
1105	66-67	0.33792736326439154
1105	68-69	0.3053460200029221
1105	70-71	0.24349730950813608
1105	72-73	0.24206384205361786
1105	74-75	0.30194419153090735
1105	76-77	0.38809920532084163
1105	78-79	0.2693813764979254
1105	80-81	0.3213487063447076
1105	82-83	0.297212041257211
1105	84-85	0.3258375047736983
1105	86-87	0.2993101053584013
1105	88-89	0.2734975251311518
1105	90-91	0.35002202809839744
1105	92-93	0.3602493489393339
1105	94-95	0.2956482133067446
1105	96-97	0.28113366388089034
1105	98-99	0.2777867775395748
1105	100	0.3019842216958004
1106	1	0.09236992510502873
1106	2	0.09339901614221802
1106	3	0.08957695482220984
1106	4	0.11040075459564491
1106	5	0.13777270408731113
1106	6	0.24791873804137055
1106	7	0.2532886057060395
1106	8	0.14567806082575885
1106	9	0.28618367638144804
1106	10-11	0.2496589564238647
1106	12-13	0.29007884285135077
1106	14-15	0.347264240795397
1106	16-17	0.3847083735485768
1106	18-19	0.33772990914402357
1106	20-21	0.32002076623539466
1106	22-23	0.32637251509989795
1106	24-25	0.3790983659441167
1106	26-27	0.27686462425557323
1106	28-29	0.36491469137391874
1106	30-31	0.28076204866107446
1106	32-33	0.3525229679552808
1106	34-35	0.4226858106785869
1106	36-37	0.3721313621708333
1106	38-39	0.32792778974917525
1106	40-41	0.36623571387016085
1106	42-43	0.35276223143812047
1106	44-45	0.3099038200064115
1106	46-47	0.33189696834065785
1106	48-49	0.377373618086871
1106	50-51	0.3551771335128393
1106	52-53	0.3878201822329075
1106	54-55	0.4191960691101926
1106	56-57	0.33918120849482847
1106	58-59	0.43052702747181115
1106	60-61	0.45426913139338865
1106	62-63	0.40167393355347514
1106	64-65	0.3752728426570542
1106	66-67	0.373030083501817
1106	68-69	0.3810954747016666
1106	70-71	0.29149979746222243
1106	72-73	0.393517780394113
1106	74-75	0.3331530315061073
1106	76-77	0.37903405346517616
1106	78-79	0.26102066828790527
1106	80-81	0.2772822208645991
1106	82-83	0.29342833973379356
1106	84-85	0.22533512144100243
1106	86-87	0.24238088013288106
1106	88-89	0.2422128539478159
1106	90-91	0.30129080412866927
1106	92-93	0.2998064941224641
1106	94-95	0.27189659763959995
1106	96-97	0.20461873990337764
1106	98-99	0.18398465367071282
1106	100	0.1906071435158232
1107	1	0.17407935866408408
1107	2	0.15566621441855943
1107	3	0.14439288890927315
1107	4	0.1800040758125121
1107	5	0.17520282777606155
1107	6	0.28081327285736535
1107	7	0.28136316203725187
1107	8	0.33171888344371325
1107	9	0.386888197919923
1107	10-11	0.3457422149462914
1107	12-13	0.31475363700295844
1107	14-15	0.3248106473275669
1107	16-17	0.4017032436814105
1107	18-19	0.3697010367804694
1107	20-21	0.3341604088437222
1107	22-23	0.331971483312131
1107	24-25	0.43470102129440136
1107	26-27	0.4522311140505124
1107	28-29	0.47002682918692074
1107	30-31	0.4352450330917037
1107	32-33	0.40707650708146303
1107	34-35	0.3948146605538625
1107	36-37	0.4306767327590322
1107	38-39	0.397150656681184
1107	40-41	0.4655208645271145
1107	42-43	0.416189364617928
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2209	5	0.23768393566834334
2209	6	0.211464732337447
2209	7	0.30100103722563176
2209	8	0.2548058231254444
2209	9	0.3089073937292852
2209	10-11	0.256897602216263
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2209	20-21	0.31391828096247565
2209	22-23	0.3768628386111459
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2209	26-27	0.30762193154753703
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2210	3	-0.44604143514503747
2210	4	-0.5155813574049617
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2210	9	-0.6116183565407525
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2210	16-17	-0.8295353562582335
2210	18-19	-0.6121407708988968
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2210	26-27	-0.6209859668790543
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2210	30-31	-0.6919064760207618
2210	32-33	-0.6374729083159849
2210	34-35	-0.6798120180765608
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2211	2	0.11936798640495638
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2211	7	0.09987452157245258
2211	8	0.07953063231610003
2211	9	0.09604408797232367
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2211	24-25	0.11418283385313543
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2211	28-29	0.1997612708359071
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2211	34-35	0.21522422887340298
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2211	78-79	0.28945463474079247
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2211	86-87	0.4194831181685039
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2211	90-91	0.3799273632514222
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2212	24-25	-0.3192879207969952
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2212	60-61	-0.15265335359125487
2212	62-63	-0.15467786176304088
2212	64-65	-0.2195974539230292
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2212	80-81	-0.2598740656308891
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2212	98-99	-0.1668818264192069
2212	100	-0.29436455735319456
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2213	2	-0.23274784198051535
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2213	86-87	-0.3379114992867507
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2214	3	-0.14914674148922558
2214	4	-0.17992989047423436
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2214	54-55	-0.24335280174489782
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2214	60-61	-0.23121484201477216
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2214	78-79	-0.2168813190454415
2214	80-81	-0.20553256255197283
2214	82-83	-0.16510036953626184
2214	84-85	-0.17189200594352272
2214	86-87	-0.16479360127812015
2214	88-89	-0.13022197579224226
2214	90-91	-0.11179458106727935
2214	92-93	-0.08780590444718328
2214	94-95	-0.07063520969969517
2214	96-97	-0.10014039855078849
2214	98-99	-0.04562080272630098
2214	100	-0.07189486447392213
2215	1	-0.24430163712089126
2215	2	-0.36695714424538295
2215	3	-0.34452062344556467
2215	4	-0.33599468263365395
2215	5	-0.3507845821662343
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2215	7	-0.515318028943895
2215	8	-0.409710008433926
2215	9	-0.5839642867052959
2215	10-11	-0.5728013494608462
2215	12-13	-0.5876834696170121
2215	14-15	-0.7626000656031806
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2215	18-19	-0.654057492381348
2215	20-21	-0.6303541521451166
2215	22-23	-0.7320815852891656
2215	24-25	-0.7905415037737953
2215	26-27	-0.6942446135246882
2215	28-29	-0.6520922134413354
2215	30-31	-0.7094863184662614
2215	32-33	-0.6685044813054049
2215	34-35	-0.7497657174893106
2215	36-37	-0.7979932017203168
2215	38-39	-0.6250012268953995
2215	40-41	-0.7915069205750029
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2215	44-45	-0.6262779890870647
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2215	54-55	-0.6559467649887907
2215	56-57	-0.6266836996663514
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2215	60-61	-0.4890438662922847
2215	62-63	-0.534536237103687
2215	64-65	-0.546083330287388
2215	66-67	-0.5067335604749559
2215	68-69	-0.5515401971667515
2215	70-71	-0.565145694824011
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2215	76-77	-0.47565659178273023
2215	78-79	-0.5121580943220607
2215	80-81	-0.5343221018772013
2215	82-83	-0.4923582909945452
2215	84-85	-0.44213377863743375
2215	86-87	-0.40290682613742845
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2215	90-91	-0.392118796737563
2215	92-93	-0.392627935004775
2215	94-95	-0.4480495383297658
2215	96-97	-0.43947106760986543
2215	98-99	-0.5827170836169273
2215	100	-0.5212933637806678
2216	1	-1.166033443164416
2216	2	-1.140244380153156
2216	3	-1.1862865600162245
2216	4	-1.4645777979272339
2216	5	-1.3930753314362505
2216	6	-2.87098484870198
2216	7	-2.774032145107384
2216	8	-3.6493645980583835
2216	9	-3.1163274695687235
2216	10-11	-2.342973215926108
2216	12-13	-2.051488420692941
2216	14-15	-2.347724773946908
2216	16-17	-2.7351281869324353
2216	18-19	-2.3872437401981372
2216	20-21	-2.1907626563699196
2216	22-23	-2.318552927953256
2216	24-25	-2.5633672315657776
2216	26-27	-2.515942954961467
2216	28-29	-2.9985711593989492
2216	30-31	-2.589632681866604
2216	32-33	-2.783704746324865
2216	34-35	-3.753006073444837
2216	36-37	-2.7838922111005218
2216	38-39	-2.7505758782565763
2216	40-41	-3.3017621998906677
2216	42-43	-3.0482156828714437
2216	44-45	-2.795875457072306
2216	46-47	-2.612274005352994
2216	48-49	-2.808546233158907
2216	50-51	-3.1698313179904574
2216	52-53	-3.5308238098122544
2216	54-55	-2.889318690796827
2216	56-57	-2.897644407834754
2216	58-59	-3.9716778938771
2216	60-61	-4.5952618768256315
2216	62-63	-3.7067267274233977
2216	64-65	-3.679532974905065
2216	66-67	-3.2777906163650634
2216	68-69	-3.722827986041331
2216	70-71	-3.4797817398301163
2216	72-73	-3.9242927848430114
2216	74-75	-4.366196130028175
2216	76-77	-4.971373901835264
2216	78-79	-3.98255727987614
2216	80-81	-3.7999390096801022
2216	82-83	-4.036193280137514
2216	84-85	-4.081560037648433
2216	86-87	-3.753797304993192
2216	88-89	-3.714098024385148
2216	90-91	-3.884271451821146
2216	92-93	-3.9044972915402383
2216	94-95	-3.761803280970927
2216	96-97	-4.051299457397512
2216	98-99	-3.8605104510498833
2216	100	-3.4557039146569863
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	76029.0
3	12573.0
4	8208.0
5	7091.0
6	6470.0
7	6910.0
8	7595.0
9	8642.0
10	9750.0
11	11058.0
12	12601.0
13	13827.0
14	15513.0
15	16585.0
16	16697.0
17	17534.0
18	17108.0
19	15688.0
20	15718.0
21	16611.0
22	18483.0
23	22010.0
24	27267.0
25	33771.0
26	41275.0
27	48558.0
28	59290.0
29	75183.0
30	91983.0
31	110166.0
32	141601.0
33	188279.0
34	261697.0
35	382547.0
36	635789.0
37	1125245.0
38	1481358.0
39	539329.0
40	1697.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.521403942849446	20.276043771639344	13.883627015547631	23.318925269963582
2	15.493697751184346	20.193055347310374	40.70103102363761	23.61221587786767
3	18.307033000811348	25.312351446309794	30.360871538810713	26.019744014068145
4	12.559467769273274	16.81634136769257	39.090814208927654	31.533376654106505
5	13.020301266273432	37.87269566187146	35.77238533087318	13.33461774098192
6	30.84270943078577	38.086784639731775	17.724680068878683	13.34582586060377
7	26.836214230057116	32.593521914116394	22.840721439447552	17.729542416378933
8	24.559738632035877	36.78207940226071	20.652801554396643	18.005380411306763
9	24.7467777563319	17.00594050912522	21.74912608033627	36.49815565420661
10-11	23.65742566790273	27.083907086866628	29.302660030099386	19.956007215131258
12-13	24.012383733830145	24.656778611805453	29.25375636381606	22.077081290548342
14-15	21.50859764152832	26.92933127548482	26.281764879131188	25.28030620385568
16-17	21.134404291026808	30.22846922722666	26.985957195170485	21.651169286576046
18-19	21.05868717043416	29.286512819307646	28.858349203903945	20.796450806354258
20-21	22.14861629676651	28.49589424765761	28.396520886902554	20.958968568673328
22-23	21.800962308482365	28.353769089213248	28.404515197293257	21.440753405011133
24-25	21.61962683437743	28.500497393920348	28.409525194098894	21.470350577603327
26-27	21.410117085321257	28.48524830748385	28.4294104201772	21.675224187017697
28-29	21.445150064965915	28.483521907689873	28.578211930335236	21.493116097008976
30-31	21.739049237828866	28.78228201971665	28.172386059005188	21.3062826834493
32-33	21.36632583847283	28.60201108560611	28.53154543957437	21.50011763634669
34-35	21.572856583620766	28.26629051669778	28.569329998951865	21.591522900729597
36-37	21.845210885175543	28.56798338180426	28.0913754151779	21.495430317842292
38-39	21.67568894823524	28.704812344168175	28.254133117551522	21.365365590045062
40-41	21.517547944867648	28.418152659418006	28.471863563759158	21.592435831955193
42-43	21.41587037212436	28.47956738287009	28.409120558427798	21.695441686577755
44-45	21.50056011053348	28.55575474369437	28.365682616897885	21.57800252887426
46-47	21.54131228813461	28.625981959062724	28.23033906146882	21.60236669133385
48-49	21.463088258184097	28.499017391728575	28.274725013541357	21.763169336545968
50-51	21.541082208915892	28.376681264949404	28.24841426514893	21.833822260985773
52-53	21.494241493769707	28.444551033807613	28.287225491187733	21.77398198123495
54-55	21.55096987441956	28.71804554006451	28.115519634810255	21.615464950705675
56-57	21.47629418375682	28.509874169542748	28.13192531697077	21.88190632972966
58-59	21.411078099287387	28.144745533999433	28.4247855961424	22.019390770570784
60-61	21.61134348586434	28.45861912568716	28.123066646222934	21.806970742225573
62-63	21.430800761028873	28.524295390214043	28.148445127729133	21.896458721027948
64-65	21.48965583298368	28.282583429175535	28.243233921919998	21.984526815920788
66-67	21.397176240505623	28.31298883619993	28.106875265370775	22.182959657923668
68-69	21.650129063623286	28.42399238654629	28.035429972764803	21.890448577065623
70-71	21.64715326390522	28.224974320080804	28.154269657379484	21.97360275863449
72-73	21.40611781810592	28.33905648731536	28.27966566011113	21.975160034467585
74-75	21.466572658462603	28.29552826942911	28.205624212658208	22.03227485945008
76-77	21.539044243796077	28.27217070596772	28.078172030684172	22.110613019552034
78-79	21.54210940576335	28.35480160585615	28.14529976312094	21.957789225259557
80-81	21.518906133969086	28.190572894007758	28.222104933875197	22.068416038147962
82-83	21.35639576337972	28.240850111776865	28.34270019317473	22.060053931668683
84-85	21.453258475055208	28.089831720770935	28.229299715908457	22.227610088265397
86-87	21.577138094452227	28.21519559983141	28.156044901991674	22.05162140372469
88-89	21.470257291541294	28.077447744538564	28.256866404847663	22.19542855907248
90-91	21.385668230929518	27.978777015701006	28.306387625048696	22.329167128320783
92-93	21.508145454382998	28.129201448378616	28.149555590799537	22.21309750643885
94-95	21.45171806476787	27.876958003933456	28.274038302165415	22.397285629133258
96-97	21.511393177527484	27.900958530377277	28.2459015573439	22.341746734751336
98-99	21.502559248953506	27.933266234777776	28.16335032591748	22.400824190351244
100	21.54973010517109	27.82203376507931	28.22015900714146	22.408077122608137
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1698.0
1	1316.0
2	1182.0
3	1501.0
4	1489.0
5	1273.5
6	1028.0
7	914.0
8	931.0
9	894.5
10	801.0
11	755.5
12	778.0
13	839.5
14	944.0
15	1106.0
16	1329.0
17	1683.0
18	2343.5
19	3294.0
20	4658.5
21	6799.5
22	9771.0
23	14171.0
24	20497.5
25	28948.5
26	40044.0
27	53359.5
28	68929.5
29	87087.0
30	104553.5
31	119919.0
32	132777.0
33	144006.0
34	156441.0
35	167018.5
36	175970.5
37	186508.5
38	195698.0
39	200663.0
40	203576.5
41	205243.5
42	205044.5
43	204080.0
44	200571.0
45	196696.5
46	192800.5
47	186596.5
48	179921.5
49	173852.0
50	168758.0
51	161261.0
52	150117.5
53	140193.5
54	132716.5
55	126106.5
56	116694.0
57	105165.0
58	96008.5
59	87163.0
60	76715.0
61	65878.0
62	56292.5
63	47514.0
64	38707.0
65	31179.0
66	25101.5
67	19693.5
68	15289.5
69	11945.0
70	9241.0
71	6930.0
72	5160.0
73	3685.0
74	2611.5
75	1812.0
76	1172.0
77	807.0
78	589.5
79	433.5
80	303.5
81	207.0
82	155.5
83	113.5
84	93.0
85	81.5
86	65.0
87	50.5
88	39.0
89	31.0
90	28.0
91	19.5
92	14.5
93	14.5
94	15.0
95	15.0
96	14.0
97	7.5
98	5.0
99	14.5
100	32.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01554199769335317
2	0.036353983110314604
3	0.03780099668866127
4	0.04051638019370688
5	0.04323176369875249
6	0.04271369710897406
7	0.040302007811729594
8	0.040248414716235276
9	0.040302007811729594
10-11	0.04060570201953075
12-13	0.04165076738167002
14-15	0.04015909289041141
16-17	0.036782727874269165
18-19	0.039507043561897166
20-21	0.0388014011378886
22-23	0.0398911274129398
24-25	0.03698816807366407
26-27	0.037032828986576005
28-29	0.037684878315090244
30-31	0.037684878315090244
32-33	0.037541963393772054
34-35	0.03743477720278341
36-37	0.03469259714999064
38-39	0.03501415572295657
40-41	0.03640757620580892
42-43	0.036193203823831635
44-45	0.03511240973136282
46-47	0.034067344369223554
48-49	0.03421025929054175
50-51	0.03548756139982307
52-53	0.03597883144185435
54-55	0.03501415572295657
56-57	0.03345995595362125
58-59	0.034469292585430966
60-61	0.0337636501614224
62-63	0.03377258234400479
64-65	0.030941080465388147
66-67	0.03201294237527457
68-69	0.033093736467743384
70-71	0.03437103857702471
72-73	0.03133409649901317
74-75	0.03162885852423194
76-77	0.0325399411476354
78-79	0.03291509281609565
80-81	0.032879364085766104
82-83	0.03154846888099046
84-85	0.032664991703788816
86-87	0.0332187870238968
88-89	0.03368326051818092
90-91	0.03422812365570652
92-93	0.03257566987796495
94-95	0.03377258234400479
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5597736.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.852309558299915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.69218355645202	48.83133171150381
2	13.597332734505402	17.09247532392676
3	3.9768223153057733	7.498574016598603
4	1.661771584581938	4.177847281973221
5	0.8569292134534306	2.692999009676274
6	0.5197339972150121	1.9599889248558315
7	0.3305541549149225	1.4543264449346454
8	0.23197099444114017	1.1663930200928967
9	0.17188799607280644	0.9723201784671484
>10	0.8921250431677985	9.984316698560464
>50	0.04949854536918212	2.112668285949256
>100	0.01884795411616114	1.8898016468484842
>500	3.1341836000975534E-4	0.12619905799941872
>1k	2.8492044489871644E-5	0.04075839861314981
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.786436516477376E-5	0.0	0.0	0.0
6	0.0	1.786436516477376E-5	0.0	0.0	0.0
7	0.0	1.786436516477376E-5	0.0	0.0	0.0
8	0.0	1.786436516477376E-5	0.0	0.0	0.0
9	0.0	1.786436516477376E-5	0.0	0.0	0.0
10-11	0.0	2.6796547747160636E-5	0.0	0.0	0.0
12-13	0.0	3.572873032954752E-5	0.0	0.0	0.0
14-15	0.0	4.46609129119344E-5	0.0	8.93218258238688E-6	0.0
16-17	0.0	7.145746065909503E-5	0.0	1.786436516477376E-5	0.0
18-19	0.0	8.93218258238688E-5	0.0	3.572873032954752E-5	0.0
20-21	0.0	8.93218258238688E-5	0.0	6.252527807670816E-5	0.0
22-23	8.93218258238688E-6	8.93218258238688E-5	0.0	2.7689766005399325E-4	0.0
24-25	1.786436516477376E-5	1.0718619098864256E-4	0.0	7.235067891733372E-4	0.0
26-27	1.786436516477376E-5	1.2505055615341632E-4	0.0	0.0015274032215881563	0.0
28-29	1.786436516477376E-5	1.2505055615341632E-4	0.0	0.004948429150642331	0.0
30-31	1.786436516477376E-5	1.339827387358032E-4	0.0	0.015756370075330456	0.0
32-33	3.572873032954752E-5	1.4291492131819007E-4	0.0	0.0418026144855706	0.0
34-35	3.572873032954752E-5	1.4291492131819007E-4	0.0	0.08239045213993658	0.0
36-37	3.572873032954752E-5	1.6077928648296382E-4	0.0	0.14325434425632078	0.0
38-39	3.572873032954752E-5	1.8757583423012448E-4	0.0	0.22449254484312942	0.0
40-41	3.572873032954752E-5	1.9650801681251134E-4	0.0	0.3064721165842762	0.0
42-43	5.359309549432127E-5	2.3223674714205886E-4	0.0	0.3910241569091504	0.0
44-45	7.145746065909503E-5	2.5010111230683264E-4	0.0	0.4925384119579773	0.0
46-47	7.145746065909503E-5	2.6796547747160636E-4	0.0	0.6118009137980069	0.0
48-49	7.145746065909503E-5	3.036942078011539E-4	0.0	0.7363244711790624	0.0
50-51	7.145746065909503E-5	3.572873032954752E-4	0.0	0.8596153873637484	0.0
52-53	7.145746065909503E-5	3.572873032954752E-4	0.0	0.9790654650380082	0.0
54-55	7.145746065909503E-5	3.572873032954752E-4	0.0	1.1107883615804675	0.0
56-57	7.145746065909503E-5	3.572873032954752E-4	0.0	1.2674588440755334	0.0
58-59	7.145746065909503E-5	3.930160336250227E-4	0.0	1.433570643560182	0.0
60-61	7.145746065909503E-5	3.930160336250227E-4	0.0	1.5996020534015896	0.0
62-63	7.145746065909503E-5	3.930160336250227E-4	0.0	1.76381129799619	0.0
64-65	7.145746065909503E-5	3.930160336250227E-4	0.0	1.9401772430854187	0.0
66-67	7.145746065909503E-5	3.930160336250227E-4	0.0	2.140999504085223	0.0
68-69	7.145746065909503E-5	4.1088039878979646E-4	0.0	2.358810776356727	0.0
70-71	7.145746065909503E-5	4.2874476395457023E-4	0.0	2.586518906929516	0.0
72-73	7.145746065909503E-5	5.448631375255997E-4	0.0	2.819157602287782	0.0
74-75	7.145746065909503E-5	5.537953201079865E-4	0.0	3.0516176539944	0.0
76-77	8.038964324148191E-5	5.716596852727603E-4	0.0	3.2999055332370086	0.0
78-79	8.93218258238688E-5	5.716596852727603E-4	0.0	3.5793577975095645	0.0
80-81	8.93218258238688E-5	5.716596852727603E-4	0.0	3.872931842444874	0.0
82-83	8.93218258238688E-5	5.89524050437534E-4	0.0	4.169623219101437	0.0
84-85	8.93218258238688E-5	6.252527807670816E-4	0.0	4.466010901550199	0.0
86-87	8.93218258238688E-5	6.341849633494684E-4	0.0	4.780066441146921	0.0
88	8.93218258238688E-5	6.788458762614028E-4	0.0	5.032391666916768	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5205	0.0	44.167713	1
GTATCAA	8950	0.0	35.509163	1
TCAACGC	9430	0.0	32.053917	4
ATCAACG	9495	0.0	31.933506	3
CAACGCA	10070	0.0	29.92363	5
AACGCAG	10670	0.0	28.32451	6
TATCAAC	11095	0.0	27.836838	2
GTACATG	14300	0.0	26.695421	1
TACATGG	14270	0.0	25.069319	2
ACGCAGA	12295	0.0	24.274885	7
CGCAGAG	12510	0.0	23.707405	8
ACATGGG	14610	0.0	23.68151	3
CATGGGG	10235	0.0	20.484665	4
GCAGAGT	14505	0.0	19.507185	9
GTCGTAC	415	0.0	19.258286	1
GTGGTAT	3585	0.0	18.490417	1
AGAGTAC	12230	0.0	17.717005	10-11
TATACTG	3550	0.0	16.949907	5
CATGGGA	4800	0.0	16.746988	4
CAGAGTA	13790	0.0	16.684158	10-11
>>END_MODULE