FastQCFastQC Report
Wed 25 May 2016
SRR2049349_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049349_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5597736
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126350.22571625385691646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121300.2166947494487057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73520.13133881269141667No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86950.056.2870521
GTATCAA157050.041.3112641
ATCAACG194900.032.938163
TCAACGC196150.032.8480534
CAACGCA202400.031.8801655
TATCAAC203950.031.5917912
AACGCAG211950.030.5758846
GTGGTAT40950.026.291111
ACGCAGA244900.026.270267
CGCAGAG247050.025.8893478
TGGTATC41850.023.4699062
GTACATG139650.023.2292651
GCAGAGT269150.023.2216919
TACATGG139550.022.024622
GAGTACT190150.020.669212-13
CAGAGTA261500.020.35813110-11
ACATGGG144800.020.349773
AGAGTAC245100.020.34841310-11
AGTACTT198500.019.2550112-13
TAGACCG2502.434099E-718.7983825
Produced by FastQC (version 0.11.5)