##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049349_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5597736
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8543407549052	31.0	31.0	34.0	30.0	34.0
2	31.917405000878926	33.0	31.0	34.0	30.0	34.0
3	31.9046737824006	33.0	31.0	34.0	30.0	34.0
4	35.658284706531354	37.0	35.0	37.0	33.0	37.0
5	35.756133908422974	37.0	35.0	37.0	35.0	37.0
6	35.823451481098786	37.0	35.0	37.0	35.0	37.0
7	35.78423759176924	37.0	35.0	37.0	35.0	37.0
8	35.7960173541589	37.0	35.0	37.0	35.0	37.0
9	37.52940956843981	39.0	37.0	39.0	35.0	39.0
10-11	37.499172433283746	39.0	37.0	39.0	35.0	39.0
12-13	37.452512497909865	39.0	37.0	39.0	34.5	39.0
14-15	38.750208923750606	40.0	38.0	41.0	35.0	41.0
16-17	38.782294842057574	40.0	38.0	41.0	35.0	41.0
18-19	38.84625480730067	40.0	38.0	41.0	35.0	41.0
20-21	38.87258366596781	40.0	38.0	41.0	35.0	41.0
22-23	38.84112094603961	40.0	38.0	41.0	35.0	41.0
24-25	38.82391934882246	40.0	38.0	41.0	35.0	41.0
26-27	38.71396310937136	40.0	38.0	41.0	34.5	41.0
28-29	38.63165027789806	40.0	38.0	41.0	34.0	41.0
30-31	38.531348387991144	40.0	38.0	41.0	34.0	41.0
32-33	38.402472267359514	40.0	38.0	41.0	34.0	41.0
34-35	38.30686075584843	40.0	38.0	41.0	34.0	41.0
36-37	38.200056326343365	40.0	38.0	41.0	34.0	41.0
38-39	38.111313127307184	40.0	38.0	41.0	33.5	41.0
40-41	38.00789265517345	40.0	38.0	41.0	33.0	41.0
42-43	37.87861610479665	40.0	37.5	41.0	33.0	41.0
44-45	37.71023919313094	40.0	37.0	41.0	33.0	41.0
46-47	37.49186492539127	40.0	37.0	41.0	33.0	41.0
48-49	37.2922015257597	40.0	37.0	41.0	32.0	41.0
50-51	37.1496027143831	40.0	37.0	41.0	32.0	41.0
52-53	37.014031744262326	40.0	36.5	41.0	31.0	41.0
54-55	36.873840959988115	40.0	36.0	41.0	31.0	41.0
56-57	36.73240288573809	39.0	36.0	41.0	31.0	41.0
58-59	36.54134912757586	39.0	35.0	41.0	31.0	41.0
60-61	36.364021365066165	39.0	35.0	41.0	31.0	41.0
62-63	36.16241226810267	39.0	35.0	41.0	30.5	41.0
64-65	35.934923422612286	38.5	35.0	40.5	30.0	41.0
66-67	35.66360935921237	38.0	35.0	40.0	30.0	41.0
68-69	35.37306412806892	37.0	35.0	40.0	29.5	41.0
70-71	35.044425996510014	37.0	34.5	39.5	29.0	41.0
72-73	34.74750461615196	36.0	34.0	39.0	29.0	41.0
74-75	34.44150009932587	36.0	34.0	39.0	29.0	41.0
76-77	34.11345327825392	35.0	34.0	38.5	29.0	40.0
78-79	33.777290050834836	35.0	34.0	37.0	29.0	39.0
80-81	33.46166048202345	35.0	34.0	37.0	29.0	39.0
82-83	33.162138943315654	35.0	34.0	36.5	28.0	39.0
84-85	32.8914148505753	35.0	34.0	36.0	28.0	37.5
86-87	32.658784730112316	35.0	33.0	36.0	27.5	37.0
88-89	32.43209710139956	35.0	33.0	35.0	27.0	37.0
90-91	32.23648837672945	35.0	33.0	35.0	27.0	36.0
92-93	32.055915552287566	35.0	33.0	35.0	27.0	36.0
94-95	31.897741426176584	35.0	33.0	35.0	26.5	36.0
96-97	31.70139168763943	35.0	33.0	35.0	26.0	36.0
98-99	31.555209731219907	35.0	33.0	35.0	25.5	35.0
100	31.438841167214747	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18901776749972043
1101	2	0.1591101411893412
1101	3	0.36038921706336424
1101	4	0.21915021155361103
1101	5	0.2039824942656665
1101	6	0.20427009120047757
1101	7	0.01774062836463486
1101	8	0.09011384335637729
1101	9	0.2439154424706942
1101	10-11	0.22006553993131917
1101	12-13	0.18165778878471173
1101	14-15	0.3455611743887985
1101	16-17	0.21132816293442858
1101	18-19	0.3043115612608034
1101	20-21	0.32858643576661706
1101	22-23	0.3051687491939248
1101	24-25	0.3126479222179128
1101	26-27	0.38679438302797564
1101	28-29	0.39793199651006717
1101	30-31	0.37598170702584355
1101	32-33	0.4303748473773865
1101	34-35	0.4281771917915762
1101	36-37	0.43952809165990914
1101	38-39	0.459505808337461
1101	40-41	0.4257050580385595
1101	42-43	0.49658657528070194
1101	44-45	0.49017618113925465
1101	46-47	0.4870923933103981
1101	48-49	0.49584071759771575
1101	50-51	0.4395677915942855
1101	52-53	0.4797648295363359
1101	54-55	0.5078249317896066
1101	56-57	0.49784356603050384
1101	58-59	0.4845105015535083
1101	60-61	0.5247203524753843
1101	62-63	0.5349988298051045
1101	64-65	0.48926331220182107
1101	66-67	0.44960907789857885
1101	68-69	0.43638501382722694
1101	70-71	0.43825208265644733
1101	72-73	0.41089080846407455
1101	74-75	0.4508068714270408
1101	76-77	0.41582562989469807
1101	78-79	0.46705368263091174
1101	80-81	0.5115776132668373
1101	82-83	0.45213901521674416
1101	84-85	0.454951343845309
1101	86-87	0.36686314592683544
1101	88-89	0.518478812608663
1101	90-91	0.5296192088588043
1101	92-93	0.5155446363723328
1101	94-95	0.5218730753113441
1101	96-97	0.5190360684061304
1101	98-99	0.5568860314757806
1101	100	0.6248408326238319
1102	1	0.2072871797227691
1102	2	0.189313109823825
1102	3	0.37786134243827973
1102	4	0.23481415373248637
1102	5	0.1757133281886638
1102	6	0.17717535298245224
1102	7	0.14362169681955095
1102	8	0.15578306211428838
1102	9	0.123703372091029
1102	10-11	0.23696516355420982
1102	12-13	0.23615409899889528
1102	14-15	0.3275623813532036
1102	16-17	0.2285532934837775
1102	18-19	0.23206953575611777
1102	20-21	0.22520693543177117
1102	22-23	0.21846111665771417
1102	24-25	0.24780909270743479
1102	26-27	0.33629469240809584
1102	28-29	0.2888076738007186
1102	30-31	0.24084399114232724
1102	32-33	0.24214229764669426
1102	34-35	0.27613589131430416
1102	36-37	0.3272820995258314
1102	38-39	0.3307069189936911
1102	40-41	0.3621555245418975
1102	42-43	0.38766143282013843
1102	44-45	0.34999176399470144
1102	46-47	0.319948193883441
1102	48-49	0.3391065154348567
1102	50-51	0.38218902630246276
1102	52-53	0.4157674234063933
1102	54-55	0.3280548548307749
1102	56-57	0.3996294546191521
1102	58-59	0.34580310214872867
1102	60-61	0.39534698089416054
1102	62-63	0.44605975604419257
1102	64-65	0.42264940189461697
1102	66-67	0.3666901139866994
1102	68-69	0.4293421247177207
1102	70-71	0.45399807351735433
1102	72-73	0.415467851752112
1102	74-75	0.4136614525869078
1102	76-77	0.38920818866490237
1102	78-79	0.38270777896794783
1102	80-81	0.40975293545645286
1102	82-83	0.4021720751770772
1102	84-85	0.3650186322523723
1102	86-87	0.3805188758814211
1102	88-89	0.3511137890564413
1102	90-91	0.38851796842419617
1102	92-93	0.47006292997477317
1102	94-95	0.4203846556340345
1102	96-97	0.43279384994556835
1102	98-99	0.4261838956354218
1102	100	0.4940300530114037
1103	1	0.18758100138749967
1103	2	0.14804491113384088
1103	3	0.29282783735086326
1103	4	0.18894862975241722
1103	5	0.18574806580189573
1103	6	0.09870284019753228
1103	7	0.1254093445747344
1103	8	0.12353762488962872
1103	9	0.16457948426444347
1103	10-11	0.20044140065665772
1103	12-13	0.19936792298921802
1103	14-15	0.26145506070707825
1103	16-17	0.28328640637099767
1103	18-19	0.26106416312845226
1103	20-21	0.27397857034287654
1103	22-23	0.28485225049067964
1103	24-25	0.2439516832065678
1103	26-27	0.2771818736960938
1103	28-29	0.3482500600680467
1103	30-31	0.32714877915644536
1103	32-33	0.33675995388841073
1103	34-35	0.2951164365888488
1103	36-37	0.31358277725250616
1103	38-39	0.34611283517844527
1103	40-41	0.3220368853458311
1103	42-43	0.35612523234007654
1103	44-45	0.39968594788031453
1103	46-47	0.37639908508327125
1103	48-49	0.2789713527871669
1103	50-51	0.2750748152486011
1103	52-53	0.33773918834156547
1103	54-55	0.25863125915802954
1103	56-57	0.23711441312070036
1103	58-59	0.34391417127451973
1103	60-61	0.3004686328332582
1103	62-63	0.3121937783473143
1103	64-65	0.33007065318236073
1103	66-67	0.29377474346029686
1103	68-69	0.28240014095273125
1103	70-71	0.2621868901633633
1103	72-73	0.3373321119348702
1103	74-75	0.2938458448535286
1103	76-77	0.3055789624043328
1103	78-79	0.3026491948023349
1103	80-81	0.30439979923747273
1103	82-83	0.30415347230749035
1103	84-85	0.29039574712295746
1103	86-87	0.3176157140456013
1103	88-89	0.3242362799617382
1103	90-91	0.32390017463915655
1103	92-93	0.3611220428094981
1103	94-95	0.36498118320491457
1103	96-97	0.41821388217361744
1103	98-99	0.4478145435398524
1103	100	0.45973667747378144
1104	1	0.11678539991101289
1104	2	0.13080946334128996
1104	3	0.21946976500756676
1104	4	0.13809173416665033
1104	5	0.12470055515575496
1104	6	0.1305330684139676
1104	7	0.15086688555063432
1104	8	0.1539791374876458
1104	9	0.13931626868225067
1104	10-11	0.15013977491008035
1104	12-13	0.12070046666639911
1104	14-15	0.19876413574107232
1104	16-17	0.2024390720639886
1104	18-19	0.1977100614178937
1104	20-21	0.19652962918386407
1104	22-23	0.19625290879213964
1104	24-25	0.18947680875912454
1104	26-27	0.18675875975502976
1104	28-29	0.21692994705909285
1104	30-31	0.19426914047396338
1104	32-33	0.272791009131538
1104	34-35	0.1968504619243916
1104	36-37	0.21663597041145977
1104	38-39	0.2756111678144464
1104	40-41	0.23159786083218137
1104	42-43	0.25397989736720206
1104	44-45	0.23212096378311742
1104	46-47	0.2304695993388819
1104	48-49	0.24470424664325208
1104	50-51	0.21382119105943076
1104	52-53	0.16869699749595668
1104	54-55	0.14176202275914562
1104	56-57	0.17751346917873434
1104	58-59	0.2508232170233029
1104	60-61	0.24930114580182305
1104	62-63	0.2644385985948787
1104	64-65	0.20408480347259683
1104	66-67	0.22402008052714706
1104	68-69	0.19102886204170488
1104	70-71	0.1899527458490411
1104	72-73	0.1760117227768987
1104	74-75	0.19277089212727816
1104	76-77	0.15021696075920232
1104	78-79	0.14574520111754907
1104	80-81	0.16065461108077272
1104	82-83	0.1959026456054147
1104	84-85	0.21736321667714975
1104	86-87	0.16356570979820617
1104	88-89	0.1479468690238619
1104	90-91	0.14441152703081173
1104	92-93	0.2589864233443038
1104	94-95	0.2763021210033223
1104	96-97	0.3355464408469828
1104	98-99	0.33723703943747196
1104	100	0.2985216729991613
1105	1	0.16767925726299637
1105	2	0.11996554864409248
1105	3	0.2561350394447004
1105	4	0.12608726179199437
1105	5	0.12483785450063323
1105	6	0.1124240759620605
1105	7	0.0798334286233029
1105	8	0.0906842552024898
1105	9	0.1691447213008459
1105	10-11	0.15632633036963028
1105	12-13	0.12010845092486733
1105	14-15	0.1537095502481236
1105	16-17	0.20131068058720558
1105	18-19	0.14761529695591946
1105	20-21	0.18156603267402005
1105	22-23	0.19117479279776006
1105	24-25	0.19519449556151613
1105	26-27	0.21589704922300967
1105	28-29	0.20669679243619754
1105	30-31	0.20331904462766204
1105	32-33	0.2520651574077135
1105	34-35	0.2974163455205243
1105	36-37	0.28985247679816695
1105	38-39	0.29398119245600896
1105	40-41	0.26658834504602424
1105	42-43	0.284496242410917
1105	44-45	0.3200128508685296
1105	46-47	0.3281185046221182
1105	48-49	0.33155729850844295
1105	50-51	0.335049027874625
1105	52-53	0.359277260135876
1105	54-55	0.3359558413005246
1105	56-57	0.319562868074307
1105	58-59	0.3500076895767208
1105	60-61	0.2966267388249335
1105	62-63	0.3266216570214553
1105	64-65	0.3013293532902708
1105	66-67	0.34673254515264773
1105	68-69	0.3115663187579827
1105	70-71	0.32195212340991475
1105	72-73	0.3816754097021118
1105	74-75	0.32953154715955435
1105	76-77	0.3216577916260519
1105	78-79	0.30291680019050204
1105	80-81	0.31829429779702423
1105	82-83	0.30369919172490256
1105	84-85	0.2634572231507093
1105	86-87	0.23454667970114684
1105	88-89	0.29384888637978435
1105	90-91	0.2883379336563081
1105	92-93	0.40112961621981214
1105	94-95	0.40435823187420183
1105	96-97	0.4592019580200457
1105	98-99	0.4201822884274371
1105	100	0.4449658224517776
1106	1	0.09352850294069981
1106	2	0.06746427796087673
1106	3	0.1869754900494982
1106	4	0.1319914725003315
1106	5	0.09124128225368366
1106	6	0.08082548448728488
1106	7	0.1341559113366344
1106	8	0.11757435104746605
1106	9	0.16613367432103132
1106	10-11	0.12701039368517542
1106	12-13	0.1002754639858594
1106	14-15	0.16688847673082563
1106	16-17	0.15992970187497946
1106	18-19	0.17271130795229084
1106	20-21	0.19027202654055486
1106	22-23	0.1479546529270337
1106	24-25	0.15032851604754427
1106	26-27	0.17596068063733128
1106	28-29	0.13864452463048593
1106	30-31	0.15988229428268852
1106	32-33	0.14896901275417918
1106	34-35	0.14874078910947475
1106	36-37	0.2046926968570162
1106	38-39	0.20608906820515216
1106	40-41	0.18207342157372608
1106	42-43	0.22571423630125054
1106	44-45	0.23115155788741504
1106	46-47	0.2460035788932018
1106	48-49	0.23990086733574856
1106	50-51	0.19213584335992095
1106	52-53	0.19322329868465005
1106	54-55	0.17884378757248243
1106	56-57	0.21718818295239828
1106	58-59	0.23334709109075646
1106	60-61	0.15668381228171313
1106	62-63	0.15364773821364963
1106	64-65	0.11471728958424876
1106	66-67	0.13328418386877416
1106	68-69	0.19109324962178675
1106	70-71	0.2280371193110824
1106	72-73	0.28416669960338226
1106	74-75	0.20757373847335003
1106	76-77	0.20810070830356153
1106	78-79	0.2384287254707118
1106	80-81	0.2308799936580641
1106	82-83	0.25669222081111087
1106	84-85	0.19241555537888644
1106	86-87	0.22206433798066882
1106	88-89	0.24405976548262487
1106	90-91	0.22077194829589786
1106	92-93	0.2774334435718231
1106	94-95	0.25596574963716634
1106	96-97	0.3084657602752685
1106	98-99	0.3301443732443836
1106	100	0.30904153594506667
1107	1	0.06516500261837521
1107	2	0.02525846507866092
1107	3	0.14947726884815893
1107	4	0.08660964835642204
1107	5	0.07626708376577085
1107	6	0.0752061988353745
1107	7	0.10103540295666136
1107	8	0.07706432167744737
1107	9	0.10354117883599656
1107	10-11	0.13194854058086491
1107	12-13	0.060503844007392615
1107	14-15	0.14437365822665527
1107	16-17	0.09370391637686026
1107	18-19	0.09190210060133097
1107	20-21	0.06673731619934387
1107	22-23	0.06588622793550059
1107	24-25	0.08874934263953804
1107	26-27	0.17177102003784483
1107	28-29	0.22772042093092892
1107	30-31	0.20061726089090826
1107	32-33	0.21071808904701328
1107	34-35	0.13521737853732674
1107	36-37	0.10404948217006904
1107	38-39	0.14632124226992715
1107	40-41	0.210064103695899
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2216	68-69	-0.4647936138578572
2216	70-71	-0.5422851347889406
2216	72-73	-0.5476879155378924
2216	74-75	-0.618240293746382
2216	76-77	-0.5236715658793969
2216	78-79	-0.6225059686217094
2216	80-81	-0.77385019421979
2216	82-83	-0.667821906214769
2216	84-85	-0.7520634849414023
2216	86-87	-0.5658968845735188
2216	88-89	-0.8967711096355231
2216	90-91	-0.6781919899853932
2216	92-93	-0.8012977751616148
2216	94-95	-0.8524032939435031
2216	96-97	-0.8634146946041739
2216	98-99	-0.9862220440928375
2216	100	-0.9607808627539605
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	275.0
3	29.0
4	49.0
5	87.0
6	234.0
7	691.0
8	1663.0
9	2813.0
10	4235.0
11	6055.0
12	8445.0
13	11810.0
14	14035.0
15	17497.0
16	22093.0
17	30338.0
18	30217.0
19	17026.0
20	13104.0
21	13141.0
22	14222.0
23	15763.0
24	18551.0
25	23281.0
26	29372.0
27	38732.0
28	50584.0
29	66541.0
30	87060.0
31	112770.0
32	145340.0
33	190512.0
34	261282.0
35	378115.0
36	615110.0
37	1118180.0
38	1582426.0
39	653168.0
40	2890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.52846238059139	20.603956393675002	14.346921976902987	23.52065924883062
2	15.418590658794912	20.7474414656211	39.83892773792833	23.99504013765565
3	17.86122103650476	25.460721977599515	30.936328544254323	25.741728441641403
4	12.60759272062068	17.26381944109487	38.35678033774405	31.7718075005404
5	12.511361736244798	37.906789459167065	35.804618152767475	13.777230651820666
6	30.511638991192154	38.48571994106189	17.23530727422658	13.767333793519379
7	26.325768131973355	33.02354737701099	22.686511118066306	17.96417337294935
8	24.649149917197988	36.6404534727718	20.3530563500301	18.357340260000107
9	24.621489830888773	16.50247528643723	21.726462269746197	37.1495726129278
10-11	23.613171467893448	27.073981338169574	28.9787067485855	20.33414044535148
12-13	24.265600480119947	24.594565926550263	29.288031485888478	21.851802107441316
14-15	21.535880957363844	26.611299535856126	26.881328854014008	24.971490652766022
16-17	20.65497551152823	29.956003641472194	27.578024401293668	21.810996445705907
18-19	20.517964994224894	28.363086595673053	30.41557801921081	20.703370390891244
20-21	21.552730242114343	27.583570974433012	30.2412568530048	20.62244193044784
22-23	21.189066436859473	27.4704451942714	30.29413141312845	21.046356955740677
24-25	21.127037713071957	27.595046382661536	30.211277701531557	21.06663820273495
26-27	20.874584798249934	27.589172076076192	30.299976034930587	21.236267090743294
28-29	20.90526970568159	27.552645160621847	30.4807606249195	21.061324508777066
30-31	21.231664015594877	27.832028877389003	30.069969359040872	20.86633774797525
32-33	20.8662637941268	27.65648677340362	30.398863465332077	21.078385967137496
34-35	21.03951583239709	27.40764963518254	30.36266837402512	21.19016615839525
36-37	21.347233009080018	27.708862472438618	29.841601041135853	21.10230347734551
38-39	21.181380576194194	27.86206368010796	29.91753686572205	21.039018877975803
40-41	21.083522904495794	27.60965085440113	30.03980905089606	21.267017190207017
42-43	21.071356379769295	27.658376027219045	29.903441566207967	21.366826026803697
44-45	21.191266649207158	27.82235748378713	29.707805722121567	21.278570144884142
46-47	21.31808845643165	27.96581291247207	29.343997184571773	21.372101446524503
48-49	21.318934740155136	27.919351895380164	29.16466323125318	21.59705013321152
50-51	21.36123062697453	27.94571234129306	28.970490466664028	21.722566565068384
52-53	21.32148773485353	28.028262010175265	28.994553289919008	21.655696965052197
54-55	21.419137710439035	28.31118912725258	28.733558878268262	21.536114284040128
56-57	21.327969460294963	28.067170129713176	28.79542504671341	21.80943536327845
58-59	21.215965305908625	27.72301892974209	29.136566271425778	21.924449492923507
60-61	21.40317207493617	28.04354948546945	28.84525925179591	21.70801918779847
62-63	21.19698288967882	28.070105547411302	28.859469129499143	21.873442433410734
64-65	21.213005774873885	27.87896917625781	28.96828303348984	21.939742015378464
66-67	21.204810215611843	27.895063061080243	28.80326909869063	22.096857624617282
68-69	21.48987994998951	27.981471786563784	28.640117135381782	21.88853112806493
70-71	21.510309051821622	27.828205217069435	28.702814119079576	21.958671612029367
72-73	21.287495423316127	27.96154433280368	28.750381967200084	22.000578276680113
74-75	21.345042977741613	27.956862370291898	28.631340031959756	22.066754620006733
76-77	21.406762028120735	27.942392966234518	28.472469286184314	22.17837571946043
78-79	21.420317131657026	27.98303890965951	28.539160440320195	22.057483518363274
80-81	21.332653274510033	27.867336721121987	28.593796755905032	22.206213248462944
82-83	21.220427737097967	27.90356085524553	28.66751619074025	22.208495216916262
84-85	21.28403422535824	27.795808001917983	28.52965643602182	22.390501336701956
86-87	21.347537996109256	27.911494216630906	28.467486910344714	22.273480876915126
88-89	21.218008742491318	27.764113696155864	28.543474006331458	22.474403555021357
90-91	21.095342911447197	27.628268995398525	28.604284765146488	22.672103328007793
92-93	21.160969618472325	27.814571074463213	28.455957392168468	22.568501914895993
94-95	21.024859722789937	27.580103789414107	28.625697813018984	22.76933867477697
96-97	21.07985645814199	27.59806064495221	28.588336536233566	22.73374636067223
98-99	21.052306248267655	27.627101066744725	28.515106671346103	22.80548601364152
100	21.115648737682307	27.4902994833537	28.518071901730014	22.875979877233977
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	720.0
1	553.0
2	750.5
3	1127.5
4	735.0
5	434.0
6	1026.5
7	2737.0
8	4439.0
9	4903.5
10	3897.5
11	2572.0
12	2393.0
13	2805.0
14	3305.0
15	3747.5
16	4330.5
17	4961.5
18	5677.5
19	6694.5
20	8237.0
21	10340.0
22	13047.0
23	17112.0
24	22979.5
25	30991.5
26	41815.5
27	54543.0
28	69287.5
29	87089.5
30	104686.5
31	119733.5
32	131752.5
33	142831.5
34	154703.0
35	164847.0
36	173050.5
37	182963.5
38	193399.0
39	198284.0
40	200517.5
41	202988.0
42	203187.0
43	202270.0
44	199355.0
45	195932.5
46	191994.0
47	186237.5
48	179857.0
49	174206.5
50	169396.0
51	161154.5
52	149213.0
53	139201.5
54	132088.0
55	124877.5
56	114921.0
57	103670.0
58	95037.0
59	86619.0
60	76216.5
61	65117.0
62	55348.0
63	46295.5
64	37434.0
65	30205.5
66	24189.0
67	18614.0
68	14100.5
69	10824.0
70	8199.0
71	6047.0
72	4387.5
73	3033.0
74	2141.0
75	1415.0
76	857.5
77	563.5
78	360.0
79	232.0
80	143.5
81	60.0
82	32.5
83	18.5
84	11.5
85	8.0
86	5.0
87	2.5
88	1.0
89	1.0
90	0.0
91	1.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036336118745149824
2	0.0
3	0.0
4	1.0718619098864256E-4
5	0.0
6	0.0
7	0.0
8	8.217607975795929E-4
9	0.0
10-11	0.0
12-13	0.044080321044079246
14-15	1.8757583423012448E-4
16-17	0.0
18-19	4.466091291193439E-5
20-21	1.6077928648296384E-4
22-23	0.0
24-25	1.5184710390057693E-4
26-27	9.825400840625568E-5
28-29	4.1088039878979646E-4
30-31	0.0
32-33	3.304907555483145E-4
34-35	8.932182582386878E-5
36-37	6.252527807670815E-5
38-39	4.0194821620740957E-4
40-41	2.501011123068326E-4
42-43	6.69913693679016E-4
44-45	3.9301603362502273E-4
46-47	1.5184710390057693E-4
48-49	0.0010629297273040386
50-51	6.520493285142422E-4
52-53	8.038964324148191E-4
54-55	6.073884156023077E-4
56-57	7.056424240085634E-4
58-59	1.8757583423012448E-4
60-61	2.1437238197728512E-4
62-63	0.001107590640215973
64-65	6.788458762614028E-4
66-67	0.001920419255213179
68-69	0.0019740123507075
70-71	6.073884156023077E-4
72-73	0.0012415733789517761
74-75	0.0012683699266989369
76-77	9.200148059858485E-4
78-79	0.003903363788503066
80-81	0.00323345009482405
82-83	0.0016435215951591858
84-85	0.003403161563889401
86-87	0.001616725047412025
88-89	0.002367028384332523
90-91	0.003269178825153598
92-93	0.0030994673560882473
94-95	0.004430362560863892
96-97	0.00366219485877862
98-99	0.0034388902942189487
100	0.001714979055818281
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5597736.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.88177637386533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.71003414653785	46.70243144541091
2	14.10514495711695	17.174925620002966
3	4.2016882572024965	7.674187346030949
4	1.7819947967831065	4.339640348685627
5	0.8913495971962287	2.7133473423717867
6	0.520355408506912	1.9008096969389494
7	0.3471160950537784	1.4793131132383464
8	0.24573098027171925	1.196843087122682
9	0.17841828577781133	0.9776179958159794
>10	0.9391610935415665	10.20495748241646
>50	0.05547159882182645	2.280270332266108
>100	0.02256458687538673	2.2632042387649984
>500	6.174005064172766E-4	0.2606303499427793
>1k	2.6459685603822975E-4	0.2569584685253057
>5k	2.939965064884695E-5	0.13159366534518102
>10k+	5.87993012976939E-5	0.4432694671209749
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12635	0.22571625385691646	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12130	0.2166947494487057	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7352	0.13133881269141667	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.786436516477376E-5	0.0
5	0.0	0.0	0.0	1.786436516477376E-5	0.0
6	0.0	0.0	0.0	1.786436516477376E-5	0.0
7	0.0	0.0	0.0	1.786436516477376E-5	0.0
8	0.0	0.0	0.0	1.786436516477376E-5	0.0
9	0.0	0.0	0.0	3.572873032954752E-5	0.0
10-11	0.0	0.0	0.0	3.572873032954752E-5	0.0
12-13	0.0	0.0	0.0	3.572873032954752E-5	0.0
14-15	0.0	3.572873032954752E-5	0.0	4.46609129119344E-5	0.0
16-17	0.0	6.252527807670816E-5	0.0	7.145746065909503E-5	0.0
18-19	0.0	7.145746065909503E-5	0.0	8.93218258238688E-5	0.0
20-21	0.0	7.145746065909503E-5	0.0	1.1611837357102943E-4	0.0
22-23	8.93218258238688E-6	7.145746065909503E-5	0.0	3.304907555483146E-4	0.0
24-25	1.786436516477376E-5	1.1611837357102943E-4	0.0	7.949642498324323E-4	0.0
26-27	1.786436516477376E-5	1.2505055615341632E-4	0.0	0.001616725047412025	0.0
28-29	1.786436516477376E-5	1.2505055615341632E-4	0.0	0.005100276254542908	0.0
30-31	1.786436516477376E-5	1.2505055615341632E-4	0.0	0.01594394590956058	0.0
32-33	2.6796547747160636E-5	1.2505055615341632E-4	0.0	0.0420705799630422	0.0
34-35	3.572873032954752E-5	1.4291492131819007E-4	0.0	0.08271201071290249	0.0
36-37	3.572873032954752E-5	1.4291492131819007E-4	0.0	0.14369202120285773	0.0
38-39	3.572873032954752E-5	1.4291492131819007E-4	0.0	0.22525178036263233	0.0
40-41	3.572873032954752E-5	1.6077928648296382E-4	0.0	0.30772262214581036	0.0
42-43	3.572873032954752E-5	1.6077928648296382E-4	0.0	0.39256049231332096	0.0
44-45	3.572873032954752E-5	1.6077928648296382E-4	0.0	0.4945660174041791	0.0
46-47	3.572873032954752E-5	1.6077928648296382E-4	0.0	0.614042891626186	0.0
48-49	3.572873032954752E-5	1.6077928648296382E-4	0.0	0.7392720914312501	0.0
50-51	3.572873032954752E-5	2.1437238197728512E-4	0.0	0.8630810742057147	0.0
52-53	3.572873032954752E-5	2.1437238197728512E-4	0.0	0.9833529126775539	0.0
54-55	3.572873032954752E-5	2.1437238197728512E-4	0.0	1.1153169781497376	0.0
56-57	3.572873032954752E-5	2.1437238197728512E-4	0.0	1.272693103068812	0.0
58-59	3.572873032954752E-5	2.1437238197728512E-4	0.0	1.4393676300561513	0.0
60-61	3.572873032954752E-5	2.1437238197728512E-4	0.0	1.6064976983551922	0.0
62-63	3.572873032954752E-5	2.3223674714205886E-4	0.0	1.7712428739047357	0.0
64-65	3.572873032954752E-5	2.3223674714205886E-4	0.0	1.9489665107464873	0.0
66-67	3.572873032954752E-5	2.5010111230683264E-4	0.0	2.150896362386508	0.0
68-69	3.572873032954752E-5	2.5010111230683264E-4	0.0	2.369913479306634	0.0
70-71	3.572873032954752E-5	2.5010111230683264E-4	0.0	2.599613486595295	0.0
72-73	3.572873032954752E-5	2.94762025218767E-4	0.0	2.8346549390682236	0.0
74-75	3.572873032954752E-5	3.036942078011539E-4	0.0	3.0686513261790127	0.0
76-77	3.572873032954752E-5	3.304907555483145E-4	0.0	3.319261572893041	0.0
78-79	3.572873032954752E-5	3.66219485877862E-4	0.0	3.6008575609853697	0.0
80-81	3.572873032954752E-5	3.930160336250227E-4	0.0	3.897057667599901	0.0
82-83	3.572873032954752E-5	3.930160336250227E-4	0.0	4.195946361171731	0.0
84-85	3.572873032954752E-5	4.1088039878979646E-4	0.0	4.495049427125538	0.0
86-87	3.572873032954752E-5	4.4660912911934395E-4	0.0	4.811954332966042	0.0
88	3.572873032954752E-5	4.823378594488915E-4	0.0	5.067084264066758	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8695	0.0	56.287052	1
GTATCAA	15705	0.0	41.311264	1
ATCAACG	19490	0.0	32.93816	3
TCAACGC	19615	0.0	32.848053	4
CAACGCA	20240	0.0	31.880165	5
TATCAAC	20395	0.0	31.591791	2
AACGCAG	21195	0.0	30.575884	6
GTGGTAT	4095	0.0	26.29111	1
ACGCAGA	24490	0.0	26.27026	7
CGCAGAG	24705	0.0	25.889347	8
TGGTATC	4185	0.0	23.469906	2
GTACATG	13965	0.0	23.229265	1
GCAGAGT	26915	0.0	23.221691	9
TACATGG	13955	0.0	22.02462	2
GAGTACT	19015	0.0	20.6692	12-13
CAGAGTA	26150	0.0	20.358131	10-11
ACATGGG	14480	0.0	20.34977	3
AGAGTAC	24510	0.0	20.348413	10-11
AGTACTT	19850	0.0	19.25501	12-13
TAGACCG	250	2.434099E-7	18.798382	5
>>END_MODULE