FastQCFastQC Report
Wed 25 May 2016
SRR2049348_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049348_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4596488
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45600.044.1244621
GTATCAA73650.034.5960921
TCAACGC81100.029.9672284
ATCAACG81350.029.8751323
CAACGCA86000.028.314765
AACGCAG92100.026.48676
TATCAAC94600.026.187642
GTACATG125850.025.4760231
TACATGG129750.023.4771022
ACGCAGA105450.022.8660377
CGCAGAG108450.021.9732258
ACATGGG135400.021.9419523
GAGTACA99200.019.2404351
GTGGTAT27900.018.8718411
CATGGGG98150.018.8225774
GCAGAGT122500.018.7625949
AGAGTAC106400.017.71425210-11
AGTACAT100650.017.2808382
CAGAGTA119450.016.44789310-11
GTATTAG23500.016.4038751
Produced by FastQC (version 0.11.5)