##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049348_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4596488 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.852265251209182 31.0 31.0 34.0 28.0 34.0 2 30.95293254328087 31.0 31.0 34.0 28.0 34.0 3 31.127828028703654 31.0 31.0 34.0 28.0 34.0 4 34.72339446986482 37.0 35.0 37.0 32.0 37.0 5 34.73188160177945 37.0 35.0 37.0 32.0 37.0 6 34.75821757829021 37.0 35.0 37.0 32.0 37.0 7 34.628436754321996 37.0 35.0 37.0 32.0 37.0 8 34.612065777175964 37.0 35.0 37.0 32.0 37.0 9 36.198826364824626 39.0 37.0 39.0 32.0 39.0 10-11 36.18920586761023 39.0 37.0 39.0 32.0 39.0 12-13 36.148951221019175 39.0 37.0 39.0 32.0 39.0 14-15 37.35763598208023 40.0 38.0 41.0 32.0 41.0 16-17 37.31436403184344 40.0 38.0 41.0 31.5 41.0 18-19 37.327152708763734 40.0 38.0 41.0 31.0 41.0 20-21 37.41030478051938 40.0 38.0 41.0 32.0 41.0 22-23 37.34934356404281 40.0 38.0 41.0 31.5 41.0 24-25 37.34729373817576 40.0 38.0 41.0 31.0 41.0 26-27 37.33470869498626 40.0 38.0 41.0 31.0 41.0 28-29 37.27861815368603 40.0 38.0 41.0 31.0 41.0 30-31 37.21588884818148 40.0 37.0 41.0 31.0 41.0 32-33 37.17102307239788 40.0 37.0 41.0 31.0 41.0 34-35 37.07197299329401 40.0 37.0 41.0 31.0 41.0 36-37 36.9907220469193 40.0 37.0 41.0 30.0 41.0 38-39 36.88834801700777 40.0 37.0 41.0 30.0 41.0 40-41 36.79070314118083 40.0 37.0 41.0 30.0 41.0 42-43 36.64455221029621 40.0 36.5 41.0 30.0 41.0 44-45 36.510132192230245 40.0 36.0 41.0 30.0 41.0 46-47 36.38583359730299 40.0 36.0 41.0 30.0 41.0 48-49 36.25246264104247 40.0 36.0 41.0 29.5 41.0 50-51 36.11005826622413 39.5 35.5 41.0 29.0 41.0 52-53 35.964657690828304 39.0 35.0 41.0 29.0 41.0 54-55 35.82608820038256 39.0 35.0 41.0 28.0 41.0 56-57 35.69392980031711 39.0 35.0 41.0 28.0 41.0 58-59 35.48487094929868 39.0 35.0 41.0 28.0 41.0 60-61 35.26838066367192 38.5 35.0 40.0 28.0 41.0 62-63 35.05834617647213 38.0 34.0 40.0 27.5 41.0 64-65 34.80934019625418 38.0 34.0 40.0 27.0 41.0 66-67 34.543355274722785 37.0 34.0 40.0 26.5 41.0 68-69 34.25808965453625 37.0 34.0 40.0 26.0 41.0 70-71 33.96987852464751 36.0 34.0 39.0 26.0 41.0 72-73 33.627407925355186 36.0 33.5 39.0 26.0 41.0 74-75 33.28115900661548 35.5 33.0 39.0 26.0 40.0 76-77 32.95137711661599 35.0 33.0 37.5 25.0 40.0 78-79 32.64233573545716 35.0 33.0 37.0 25.0 39.0 80-81 32.30234311500433 35.0 33.0 37.0 24.5 39.0 82-83 31.999709995979536 35.0 33.0 36.0 24.0 38.5 84-85 31.72027665469811 35.0 32.5 36.0 23.5 37.0 86-87 31.42000533885871 35.0 32.0 35.5 22.5 37.0 88-89 31.185498798212897 35.0 32.0 35.0 21.0 36.5 90-91 30.996627098776283 35.0 32.0 35.0 20.0 36.0 92-93 30.776003113681575 35.0 32.0 35.0 19.0 36.0 94-95 30.589685973290912 35.0 32.0 35.0 17.5 36.0 96-97 30.353569507850338 35.0 32.0 35.0 7.5 35.5 98-99 30.15944107762274 35.0 32.0 35.0 2.0 35.0 100 30.04752976620411 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3789945622943378 1101 2 0.30908321491605406 1101 3 0.36625487425511594 1101 4 0.41633810906544255 1101 5 0.4780483214916913 1101 6 0.5159321886326396 1101 7 0.4848819386305294 1101 8 0.4845264027000056 1101 9 0.6939088563930653 1101 10-11 0.6611157215530312 1101 12-13 0.6404976743917032 1101 14-15 0.7473784886189421 1101 16-17 0.7704181871533038 1101 18-19 0.7375709818393972 1101 20-21 0.7428737451779455 1101 22-23 0.6921210295327498 1101 24-25 0.7853724821875829 1101 26-27 0.7028785976428154 1101 28-29 0.6701155306029634 1101 30-31 0.7635079987649007 1101 32-33 0.6895773195062986 1101 34-35 0.7040384917240488 1101 36-37 0.6626803925037521 1101 38-39 0.6924879171314231 1101 40-41 0.7394674469381144 1101 42-43 0.65339781211663 1101 44-45 0.5975917410951652 1101 46-47 0.613323155414065 1101 48-49 0.48912683608300966 1101 50-51 0.5598773158121375 1101 52-53 0.5972144777778396 1101 54-55 0.5943423889733666 1101 56-57 0.5571954297098145 1101 58-59 0.6895630811015607 1101 60-61 0.6584199258253491 1101 62-63 0.5888933962372676 1101 64-65 0.5707382275292616 1101 66-67 0.6324248803466332 1101 68-69 0.5995268736329891 1101 70-71 0.669326417630451 1101 72-73 0.7803003095687728 1101 74-75 0.7689626132067744 1101 76-77 0.7287160468163307 1101 78-79 0.686683258141386 1101 80-81 0.7229958906762874 1101 82-83 0.7445777219620453 1101 84-85 0.715125584212096 1101 86-87 0.7626008186856161 1101 88-89 0.7925792785135641 1101 90-91 0.7843227936068153 1101 92-93 0.8161603169140328 1101 94-95 0.8278951953295106 1101 96-97 0.8790880984153198 1101 98-99 0.9016941622239933 1101 100 0.9172847678358984 1102 1 0.2562522877295095 1102 2 0.332447281257366 1102 3 0.3977547580607528 1102 4 0.38834242659240914 1102 5 0.3924480571631861 1102 6 0.47753750046020116 1102 7 0.5652473214222198 1102 8 0.578514128356133 1102 9 0.7100682667268643 1102 10-11 0.6347972612614328 1102 12-13 0.6855018207645145 1102 14-15 0.7832769711817633 1102 16-17 0.7166328154802315 1102 18-19 0.6712828939003899 1102 20-21 0.6132774698982786 1102 22-23 0.6332284259787215 1102 24-25 0.6597812163158068 1102 26-27 0.7041555132974366 1102 28-29 0.6668812154511059 1102 30-31 0.6181083934089813 1102 32-33 0.7018518962094475 1102 34-35 0.7193888488764912 1102 36-37 0.6247198994261396 1102 38-39 0.6459231736799893 1102 40-41 0.6937131536546914 1102 42-43 0.6322997512668209 1102 44-45 0.5431750478682815 1102 46-47 0.45969642787416376 1102 48-49 0.4950761715213261 1102 50-51 0.5632447172262687 1102 52-53 0.5400223795162304 1102 54-55 0.5072303871746087 1102 56-57 0.4435326487382554 1102 58-59 0.5257672220168175 1102 60-61 0.5456028922180209 1102 62-63 0.5007267044364738 1102 64-65 0.514097554826165 1102 66-67 0.6019064728006001 1102 68-69 0.6264075744881623 1102 70-71 0.6230797680410234 1102 72-73 0.609656817700035 1102 74-75 0.5778908806660112 1102 76-77 0.6141244094159575 1102 78-79 0.5613985726332373 1102 80-81 0.5532137691392336 1102 82-83 0.6090674200818427 1102 84-85 0.6328439315386944 1102 86-87 0.5838320798849317 1102 88-89 0.6209587691009837 1102 90-91 0.6850499065357489 1102 92-93 0.6281175308448681 1102 94-95 0.7353525708800603 1102 96-97 0.720732151942542 1102 98-99 0.7725214300158818 1102 100 0.743637417047637 1103 1 0.12342742412047514 1103 2 0.1983954216205177 1103 3 0.20738826104984653 1103 4 0.2442973277947047 1103 5 0.2368436883237024 1103 6 0.3574261649397741 1103 7 0.3236358681265088 1103 8 0.369767792225133 1103 9 0.4375552201324524 1103 10-11 0.4625845243545541 1103 12-13 0.5029972731346248 1103 14-15 0.5608545001078866 1103 16-17 0.5338592154136066 1103 18-19 0.4926098751998751 1103 20-21 0.46483658732417155 1103 22-23 0.507524421054498 1103 24-25 0.5119744036631246 1103 26-27 0.5218212305549557 1103 28-29 0.5688814452228073 1103 30-31 0.5625486914910098 1103 32-33 0.527676951860137 1103 34-35 0.5263097160444232 1103 36-37 0.45916964920636616 1103 38-39 0.5124542888797521 1103 40-41 0.49722342391176255 1103 42-43 0.5227915606466311 1103 44-45 0.43061076874551674 1103 46-47 0.43095517492317015 1103 48-49 0.44174087961744846 1103 50-51 0.4206832389620203 1103 52-53 0.4482326144150548 1103 54-55 0.5105665169039995 1103 56-57 0.4061341592205281 1103 58-59 0.579725918579868 1103 60-61 0.563396387349627 1103 62-63 0.5775533408887696 1103 64-65 0.5163175358558334 1103 66-67 0.49936989413059507 1103 68-69 0.536350758315713 1103 70-71 0.48692136292523003 1103 72-73 0.4643659582604087 1103 74-75 0.4698162418586165 1103 76-77 0.5599872705207076 1103 78-79 0.43312109347908745 1103 80-81 0.4455057542995249 1103 82-83 0.48592361359040126 1103 84-85 0.5742288997351253 1103 86-87 0.5558488080140798 1103 88-89 0.5569490557691736 1103 90-91 0.58409293575939 1103 92-93 0.6010181035359814 1103 94-95 0.6699690579787401 1103 96-97 0.6142275155990866 1103 98-99 0.6504552389440512 1103 100 0.6366982158922916 1104 1 0.23870223830357062 1104 2 0.24966615654181012 1104 3 0.28211841897110546 1104 4 0.30715262402956256 1104 5 0.4050687114136835 1104 6 0.40698855669913314 1104 7 0.41541128000450556 1104 8 0.40003343454650064 1104 9 0.47966807750700724 1104 10-11 0.49537865396840175 1104 12-13 0.45656628618757367 1104 14-15 0.5938097587613385 1104 16-17 0.585293513736687 1104 18-19 0.5315080875887332 1104 20-21 0.4894658232377225 1104 22-23 0.5377469955407221 1104 24-25 0.5422057611565663 1104 26-27 0.49963640528361 1104 28-29 0.5479806763032329 1104 30-31 0.516105457664537 1104 32-33 0.5563792188969359 1104 34-35 0.5358991084194713 1104 36-37 0.5163604741617362 1104 38-39 0.6143040674128457 1104 40-41 0.5986836615973132 1104 42-43 0.5733149142016885 1104 44-45 0.532572603008056 1104 46-47 0.5020716347677165 1104 48-49 0.45423699697060016 1104 50-51 0.5595066467250618 1104 52-53 0.4734638563031055 1104 54-55 0.48401339489561934 1104 56-57 0.528535445404529 1104 58-59 0.6060670687681764 1104 60-61 0.5430538809534724 1104 62-63 0.5225791153151533 1104 64-65 0.5847863319009363 1104 66-67 0.6392589090915664 1104 68-69 0.5894199721445545 1104 70-71 0.5897919864192147 1104 72-73 0.5559456238249467 1104 74-75 0.6555882977913754 1104 76-77 0.6246785906060524 1104 78-79 0.564609124503022 1104 80-81 0.5805717186516262 1104 82-83 0.5368404437239533 1104 84-85 0.6241552232806953 1104 86-87 0.5873384519370255 1104 88-89 0.5367394526800311 1104 90-91 0.5381850685828233 1104 92-93 0.5738194573073194 1104 94-95 0.5725171531471602 1104 96-97 0.5818324283521896 1104 98-99 0.6143041560346525 1104 100 0.6862875036004858 1105 1 0.1392953199456919 1105 2 0.15477671859070696 1105 3 0.18569366570215706 1105 4 0.21834537365764817 1105 5 0.20459948567963693 1105 6 0.30991430351218696 1105 7 0.22678852514139436 1105 8 0.27857276583976187 1105 9 0.3571668252980089 1105 10-11 0.36567551708492374 1105 12-13 0.32466001335269823 1105 14-15 0.38507427588299237 1105 16-17 0.37967135114326567 1105 18-19 0.37393818955382585 1105 20-21 0.32524488406626517 1105 22-23 0.30604595200353657 1105 24-25 0.2664118092835679 1105 26-27 0.33517534978089003 1105 28-29 0.36346667072205463 1105 30-31 0.3410442577751098 1105 32-33 0.37527402634009377 1105 34-35 0.43742711616416585 1105 36-37 0.39027022588197013 1105 38-39 0.33028489099871194 1105 40-41 0.40711814827198367 1105 42-43 0.37379807569313783 1105 44-45 0.2946488336723476 1105 46-47 0.3185365602186607 1105 48-49 0.29425291745492643 1105 50-51 0.4046804544938851 1105 52-53 0.3736534575850996 1105 54-55 0.3500576253491303 1105 56-57 0.36595647317823676 1105 58-59 0.37530536321872177 1105 60-61 0.3835619026927617 1105 62-63 0.34199382096100805 1105 64-65 0.3910884695607848 1105 66-67 0.4341936486107585 1105 68-69 0.3561490328094621 1105 70-71 0.3352623888631001 1105 72-73 0.3453810210050037 1105 74-75 0.36074686032214487 1105 76-77 0.45439803075149143 1105 78-79 0.35615117612738345 1105 80-81 0.3974639030737279 1105 82-83 0.34554915080920523 1105 84-85 0.3367697060041479 1105 86-87 0.3424162935400723 1105 88-89 0.3266715183482667 1105 90-91 0.3114891326830005 1105 92-93 0.3050933557952682 1105 94-95 0.3054496822497548 1105 96-97 0.32245603319707783 1105 98-99 0.34959275608384033 1105 100 0.33459590191177213 1106 1 0.09199807201972021 1106 2 0.037055605736846076 1106 3 0.08368324974595964 1106 4 0.10544483008214911 1106 5 0.020654686551971224 1106 6 0.1500926100635951 1106 7 0.10583823804908121 1106 8 0.20013583231030196 1106 9 0.1993693389350426 1106 10-11 0.21277326107916394 1106 12-13 0.22186471016816256 1106 14-15 0.28343154997507014 1106 16-17 0.24704808113775556 1106 18-19 0.24600291261146623 1106 20-21 0.25778984552047035 1106 22-23 0.325242027050912 1106 24-25 0.3168428005959143 1106 26-27 0.27470979536377627 1106 28-29 0.3237898075547818 1106 30-31 0.35589338444068375 1106 32-33 0.3516390854036331 1106 34-35 0.4038217531865058 1106 36-37 0.2669988790944089 1106 38-39 0.3076807313866752 1106 40-41 0.3116465974461704 1106 42-43 0.3515607152812734 1106 44-45 0.3265537277696211 1106 46-47 0.27398872979313893 1106 48-49 0.3046331096054615 1106 50-51 0.37042973712593863 1106 52-53 0.32698115461922583 1106 54-55 0.3328384723711153 1106 56-57 0.23651478209949062 1106 58-59 0.30896201302246595 1106 60-61 0.2713378031054745 1106 62-63 0.24418167183879547 1106 64-65 0.27699426960053586 1106 66-67 0.3055589558105254 1106 68-69 0.27134860110246706 1106 70-71 0.28639208335484767 1106 72-73 0.353015509190449 1106 74-75 0.38376329242584006 1106 76-77 0.4268862289659552 1106 78-79 0.27162842878145455 1106 80-81 0.31982130576824375 1106 82-83 0.24542132711849263 1106 84-85 0.26027854488894064 1106 86-87 0.3058037576736794 1106 88-89 0.30492534555684614 1106 90-91 0.3756360918710868 1106 92-93 0.3791686169253694 1106 94-95 0.4278170884678225 1106 96-97 0.3424467047161741 1106 98-99 0.3329416939396559 1106 100 0.3063963753985419 1107 1 0.2157844892149967 1107 2 0.16761254733612674 1107 3 0.20599812599922984 1107 4 0.1916519038764548 1107 5 0.17206574968811594 1107 6 0.20508517486599231 1107 7 0.203543039889162 1107 8 0.24046197497043664 1107 9 0.327216305111925 1107 10-11 0.3008746534831914 1107 12-13 0.30742480661091065 1107 14-15 0.34310403794690103 1107 16-17 0.36249363445642047 1107 18-19 0.3448983681890354 1107 20-21 0.31599880668845515 1107 22-23 0.3425864171090751 1107 24-25 0.33027924381114815 1107 26-27 0.353048162596032 1107 28-29 0.4022762744906814 1107 30-31 0.3659338047323004 1107 32-33 0.3843634697952041 1107 34-35 0.3877870714494094 1107 36-37 0.30580703412444876 1107 38-39 0.3416956078243629 1107 40-41 0.4437822359791497 1107 42-43 0.4011796876439391 1107 44-45 0.34301650377526727 1107 46-47 0.24103367162815204 1107 48-49 0.2848904911563963 1107 50-51 0.3664924606953761 1107 52-53 0.4103516256845836 1107 54-55 0.3980555766489644 1107 56-57 0.35181732670312726 1107 58-59 0.364054337659681 1107 60-61 0.39231589757216767 1107 62-63 0.41239495926809155 1107 64-65 0.3762665662375113 1107 66-67 0.35033465195269997 1107 68-69 0.34035423469827464 1107 70-71 0.317958851484228 1107 72-73 0.4020144501471066 1107 74-75 0.4088008529355207 1107 76-77 0.43032640321862914 1107 78-79 0.3462308245320287 1107 80-81 0.31988104395841077 1107 82-83 0.32859799729332906 1107 84-85 0.3698269971518897 1107 86-87 0.4135088012131085 1107 88-89 0.41403829758513666 1107 90-91 0.44662295382454786 1107 92-93 0.4305225079650299 1107 94-95 0.45102502221740437 1107 96-97 0.3642910186351287 1107 98-99 0.3981794858764651 1107 100 0.3697957416989581 1108 1 0.010018747202238387 1108 2 0.0628755167144881 1108 3 0.05384684609963841 1108 4 0.08987873261958157 1108 5 0.09286221002149375 1108 6 0.1657442359665211 1108 7 0.1523548316779042 1108 8 0.20827364309814556 1108 9 0.22609796008538297 1108 10-11 0.17292549580177763 1108 12-13 0.1545640367261143 1108 14-15 0.11968245288729662 1108 16-17 0.20172376144244453 1108 18-19 0.16491645317397285 1108 20-21 0.22140311014753422 1108 22-23 0.25476077948602693 1108 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-0.4220078034227299 2215 56-57 -0.4359059415543456 2215 58-59 -0.36752493305812095 2215 60-61 -0.18553770563574545 2215 62-63 -0.23679232002350403 2215 64-65 -0.2130403954387603 2215 66-67 -0.16059685654042966 2215 68-69 -0.18953253553360838 2215 70-71 -0.23579104926547245 2215 72-73 -0.2375966575508741 2215 74-75 -0.20840226832013542 2215 76-77 -0.2282796663366966 2215 78-79 -0.27018623437539446 2215 80-81 -0.3291866319894581 2215 82-83 -0.23512099844097278 2215 84-85 -0.2119843306392326 2215 86-87 -0.1347978180720979 2215 88-89 -0.14181308672745985 2215 90-91 -0.20828037009932032 2215 92-93 -0.1870206510937642 2215 94-95 -0.2517282977723774 2215 96-97 -0.24273795023602673 2215 98-99 -0.3510383140040396 2215 100 -0.324549811726083 2216 1 -1.1833681161786203 2216 2 -1.0933534001791578 2216 3 -1.2057759099680574 2216 4 -1.3394677160151787 2216 5 -1.2597978861841241 2216 6 -2.773072964620134 2216 7 -2.746964729669209 2216 8 -3.523776828235512 2216 9 -3.036317029335237 2216 10-11 -2.1588131424117876 2216 12-13 -1.8151479878519154 2216 14-15 -2.1597310392387215 2216 16-17 -2.5432217173514076 2216 18-19 -2.1430988950674177 2216 20-21 -1.98093833262066 2216 22-23 -2.092804589856833 2216 24-25 -2.276303715324758 2216 26-27 -2.308714871579191 2216 28-29 -2.812178191139445 2216 30-31 -2.3681944039826774 2216 32-33 -2.6011332198938675 2216 34-35 -3.579282107928279 2216 36-37 -2.590103373177733 2216 38-39 -2.463590007979519 2216 40-41 -3.0314044368594395 2216 42-43 -2.8681796717252652 2216 44-45 -2.654683138893084 2216 46-47 -2.4715943327210255 2216 48-49 -2.7178955557102498 2216 50-51 -2.971462198599049 2216 52-53 -3.3488689134545524 2216 54-55 -2.660743432785715 2216 56-57 -2.660112738153387 2216 58-59 -3.728669148048745 2216 60-61 -4.454291602930965 2216 62-63 -3.5398254862875618 2216 64-65 -3.5392936695841257 2216 66-67 -3.1551969562777984 2216 68-69 -3.5853795193130154 2216 70-71 -3.3300497313331654 2216 72-73 -3.8013767171228316 2216 74-75 -4.343107917037916 2216 76-77 -4.908353746458161 2216 78-79 -3.888270844518992 2216 80-81 -3.7721497383459877 2216 82-83 -4.027345465616545 2216 84-85 -4.048751524357954 2216 86-87 -3.709001667961182 2216 88-89 -3.6790031208041185 2216 90-91 -3.844950798203687 2216 92-93 -3.9083845570855367 2216 94-95 -3.738207248162638 2216 96-97 -4.034657412061605 2216 98-99 -3.761516630976871 2216 100 -3.385062545937352 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72397.0 3 12994.0 4 8156.0 5 6916.0 6 6294.0 7 6334.0 8 7088.0 9 8021.0 10 8852.0 11 10132.0 12 11104.0 13 12271.0 14 13551.0 15 14686.0 16 15260.0 17 16092.0 18 15609.0 19 13878.0 20 14022.0 21 14633.0 22 16215.0 23 19575.0 24 23899.0 25 29100.0 26 35955.0 27 42020.0 28 50663.0 29 63740.0 30 77790.0 31 92944.0 32 119065.0 33 157172.0 34 215389.0 35 314769.0 36 520349.0 37 913632.0 38 1189724.0 39 425034.0 40 1163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.71224346405478 20.066274981771006 13.889981093199927 23.33150046097429 2 15.431705259006614 19.99459806631668 40.63290117138386 23.94079550329285 3 18.412055213595632 25.013096916310108 30.219586370327033 26.355261499767224 4 12.561593134347524 16.484639669689507 38.58048760845931 32.37327958750366 5 13.086288350468678 37.97912850801155 35.48591136200143 13.448671779518339 6 31.44356296128137 37.56272577742962 17.487255689150473 13.506455572138535 7 27.292238726965955 32.49323539602969 22.3552966762027 17.859229200801654 8 24.945859627235645 36.7819571848138 20.275901516104557 17.996281671846003 9 24.886838040352846 17.042886516352848 21.4670256159456 36.603249827348705 10-11 24.003027534567163 27.087271241305494 28.759064511407395 20.150636712719944 12-13 24.228164291610373 24.481959493541396 28.97820795840201 22.311668256446225 14-15 21.67737505250809 26.704407197254127 25.986401220581868 25.631816529655914 16-17 21.385254448548448 30.10570219912025 26.68464172097379 21.824401631357514 18-19 21.336733733335585 29.09125633970192 28.57807307885471 20.99393684810779 20-21 22.322547998052055 28.26375599550558 28.248640383930006 21.16505562251236 22-23 22.026019381933303 28.120192319725827 28.241151202085348 21.61263709625552 24-25 21.856093990308153 28.23422229133587 28.22979326228242 21.679890456073554 26-27 21.597550650860015 28.292898976862425 28.300657933397027 21.808892438880534 28-29 21.651172739074003 28.318416889035824 28.390576909076525 21.639833462813648 30-31 21.894298597902058 28.557928399559447 27.99897402424842 21.54879897829008 32-33 21.58419601864336 28.358618092940404 28.375572539020798 21.68161334939544 34-35 21.77140130379402 28.13410328386113 28.364664037169174 21.729831375175678 36-37 21.98337096106713 28.407272274586443 27.952257266839087 21.657099497507343 38-39 21.81548785290443 28.521780633094306 28.07848425436552 21.58424725963574 40-41 21.75147163515331 28.279422528341776 28.21550131792608 21.75360451857883 42-43 21.685852823643838 28.347228633568232 28.168382005515465 21.798536537272465 44-45 21.769918187648905 28.373168003799094 28.10669187206447 21.750221936487534 46-47 21.775730600148798 28.467917948313925 27.97782423331586 21.778527218221413 48-49 21.773885961986604 28.327374640288454 27.996067316999383 21.902672080725555 50-51 21.796307931911265 28.26595852805658 27.970165638359624 21.96756790167253 52-53 21.77490430096914 28.29893551185175 28.013087128802727 21.91307305837638 54-55 21.834903556883344 28.53658313638887 27.884930750780068 21.743582555947714 56-57 21.797490938799957 28.363471317770745 27.912315614862326 21.92672212856697 58-59 21.716751778657244 28.07471486694226 28.157470948045177 22.051062406355317 60-61 21.906008847281026 28.37044148045489 27.896400715756613 21.827148956507468 62-63 21.7470996801193 28.460002289525764 27.865846412865913 21.927051617489017 64-65 21.770063074302325 28.223761705035557 27.994565805817327 22.011609414844795 66-67 21.697269065231374 28.258285194209225 27.89555920184612 22.14888653871328 68-69 21.963191465815633 28.37722506904688 27.793217045343134 21.866366419794357 70-71 21.963280816937512 28.215779555190313 27.899629996306707 21.921309631565467 72-73 21.82840447555339 28.33969809329238 27.956299969803887 21.87559746135034 74-75 21.88787690834503 28.36317043711031 27.88040131013395 21.86855134441071 76-77 21.924698658505175 28.365072972569767 27.726301148564016 21.983927220361046 78-79 21.89997812790064 28.4046860700843 27.85649073591453 21.838845066100532 80-81 21.913993380255743 28.3130468506268 27.877106102963715 21.89585366615374 82-83 21.82894103854452 28.411175332448522 27.920115509179134 21.839768119827824 84-85 21.885677599077237 28.273553287340313 27.81553461446386 22.025234499118586 86-87 21.991023689993575 28.426479733659637 27.758800706657254 21.82369586968953 88-89 21.875317270655685 28.302430468690577 27.837116496289493 21.985135764364248 90-91 21.842578391858854 28.2277941331056 27.834091032252967 22.095536442782578 92-93 21.912208963350977 28.42877696651796 27.723137618987614 21.93587645114345 94-95 21.89205408884626 28.223602633166823 27.82264264483206 22.061700633154853 96-97 21.875603721798033 28.28741204154128 27.800170477982324 22.036813758678363 98-99 21.896369576076342 28.3281931770517 27.752101169414562 22.023336077457397 100 21.960853590828474 28.217782794168066 27.763522933161145 22.05784068184231 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1617.0 1 1293.5 2 1170.5 3 1397.5 4 1346.5 5 1143.0 6 897.0 7 805.0 8 792.0 9 735.5 10 692.5 11 669.0 12 715.5 13 783.0 14 880.5 15 1019.0 16 1192.0 17 1468.0 18 1953.5 19 2740.0 20 3968.0 21 5750.0 22 8186.0 23 11825.5 24 16882.5 25 23330.0 26 31830.5 27 42054.5 28 54290.0 29 68119.0 30 82008.0 31 94479.5 32 104439.0 33 114060.5 34 124601.0 35 133357.0 36 140984.5 37 149273.0 38 156428.0 39 160246.5 40 162570.0 41 165042.0 42 165479.5 43 164717.0 44 162679.5 45 159800.0 46 156439.5 47 150908.0 48 145269.5 49 142154.0 50 139288.5 51 134345.5 52 126805.5 53 119666.0 54 114163.0 55 108821.0 56 101711.5 57 92194.0 58 84000.0 59 76939.0 60 68236.0 61 58330.0 62 49539.5 63 41854.5 64 34408.5 65 27946.5 66 22601.5 67 17691.0 68 13647.5 69 10633.5 70 8058.0 71 6008.0 72 4560.0 73 3301.0 74 2419.0 75 1726.5 76 1177.0 77 804.0 78 532.0 79 356.0 80 229.0 81 165.5 82 123.0 83 95.0 84 78.5 85 66.0 86 56.0 87 38.5 88 24.0 89 20.5 90 17.0 91 14.0 92 13.0 93 12.5 94 12.5 95 11.5 96 10.0 97 9.5 98 9.5 99 11.5 100 20.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01712176774963842 2 0.03996529524280277 3 0.041227128190044225 4 0.04401186297016331 5 0.04749278144531216 6 0.04536071887928349 7 0.04316338909184577 8 0.042423693915876644 9 0.04279354150386121 10-11 0.043304801404898695 12-13 0.04428380972603431 14-15 0.04259773983963409 16-17 0.03887750821931875 18-19 0.04199945697671788 20-21 0.041346784762627466 22-23 0.04245632752658116 24-25 0.039192966456129116 26-27 0.03911682136448523 28-29 0.03999792885350729 30-31 0.03997617311303761 32-33 0.03966071487622724 34-35 0.03960632552505304 36-37 0.03686510222587332 38-39 0.03712617111150948 40-41 0.0384859048908645 42-43 0.0384859048908645 44-45 0.03709353750080496 46-47 0.03595136112614675 48-49 0.03612540704990418 50-51 0.03739811786738049 52-53 0.03795288924935734 54-55 0.037137048981744324 56-57 0.03528781104182149 58-59 0.036103651309434506 60-61 0.03546185696557894 62-63 0.035896971774972544 64-65 0.03286204597945214 66-67 0.03392807726246647 68-69 0.03486357410266273 70-71 0.036310330843896475 72-73 0.03318838208649734 74-75 0.033460328842368346 76-77 0.03429792485045104 78-79 0.034656894568200765 80-81 0.03475479540031433 82-83 0.033253649307906384 84-85 0.03454811586585236 86-87 0.035146398728768576 88-89 0.03553800205722282 90-91 0.03600575047732094 92-93 0.03430880272068588 94-95 0.03569029224051058 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4596488.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.03165072203961 #Duplication Level Percentage of deduplicated Percentage of total 1 78.30578273068208 50.14048529326956 2 13.024192602567936 16.67921103328404 3 3.9945549343049715 7.673338390302476 4 1.6394263673772356 4.199007061616054 5 0.8502157199887074 2.7220358010352173 6 0.5129684409889311 1.9707729626899457 7 0.3302646246245924 1.4803172362865196 8 0.2350852249516862 1.2042316011214786 9 0.17223704838265927 0.9925760271090126 >10 0.881799908328388 9.907984266555312 >50 0.041361290107998896 1.7803972592883928 >100 0.011975063958627429 1.157022345907676 >500 1.02032873699153E-4 0.05213594626913848 >1k 3.401086245833536E-5 0.04048477526512986 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.702296187872132E-5 0.0 5 0.0 0.0 0.0 8.702296187872132E-5 0.0 6 0.0 0.0 0.0 8.702296187872132E-5 0.0 7 0.0 0.0 0.0 1.3053444281808197E-4 0.0 8 0.0 0.0 0.0 1.3053444281808197E-4 0.0 9 0.0 0.0 0.0 1.9580166422712296E-4 0.0 10-11 0.0 2.175574046968033E-5 0.0 2.2843527493164345E-4 0.0 12-13 0.0 2.175574046968033E-5 0.0 2.7194675587100415E-4 0.0 14-15 0.0 3.2633610704520494E-5 0.0 3.0458036657552464E-4 0.0 16-17 0.0 4.351148093936066E-5 0.0 3.2633610704520494E-4 0.0 18-19 0.0 5.438935117420083E-5 0.0 3.6984758798456563E-4 0.0 20-21 0.0 6.526722140904099E-5 0.0 3.6984758798456563E-4 0.0 22-23 0.0 7.614509164388116E-5 0.0 4.895041605678074E-4 0.0 24-25 0.0 1.0877870234840165E-4 0.0 6.744279545600903E-4 0.0 26-27 0.0 1.0877870234840165E-4 0.0 0.0010333976723098156 0.0 28-29 0.0 1.0877870234840165E-4 0.0 0.002501910154013238 0.0 30-31 0.0 1.0877870234840165E-4 0.0 0.007059737782411268 0.0 32-33 0.0 1.0877870234840165E-4 0.0 0.018601158101576684 0.0 34-35 0.0 1.3053444281808197E-4 0.0 0.03633208658436615 0.0 36-37 0.0 1.4141231305292215E-4 0.0 0.06309164736207296 0.0 38-39 0.0 1.7404592375744264E-4 0.0 0.09778117554097823 0.0 40-41 0.0 1.7404592375744264E-4 0.0 0.13286230704833776 0.0 42-43 0.0 1.7404592375744264E-4 0.0 0.16970565353374142 0.0 44-45 0.0 1.7404592375744264E-4 0.0 0.2126841188315949 0.0 46-47 0.0 1.7404592375744264E-4 0.0 0.2641038114316844 0.0 48-49 0.0 1.7404592375744264E-4 0.0 0.3179383912239083 0.0 50-51 0.0 1.7404592375744264E-4 0.0 0.3695212518775204 0.0 52-53 0.0 1.7404592375744264E-4 0.0 0.42126728058465507 0.0 54-55 0.0 1.7404592375744264E-4 0.0 0.4793986191196409 0.0 56-57 0.0 1.7404592375744264E-4 0.0 0.5468196588351802 0.0 58-59 0.0 1.7404592375744264E-4 0.0 0.6204519624548133 0.0 60-61 0.0 1.7404592375744264E-4 0.0 0.6940842660744464 0.0 62-63 0.0 1.8492379399228281E-4 0.0 0.7649427127841952 0.0 64-65 0.0 1.9580166422712296E-4 0.0 0.8425563169097798 0.0 66-67 0.0 1.9580166422712296E-4 0.0 0.9299600042467205 0.0 68-69 0.0 1.9580166422712296E-4 0.0 1.0241841162209062 0.0 70-71 0.0 1.9580166422712296E-4 0.0 1.126740676794979 0.0 72-73 0.0 1.9580166422712296E-4 0.0 1.229852008751029 0.0 74-75 0.0 1.9580166422712296E-4 0.0 1.334736433555358 0.0 76-77 0.0 2.2843527493164345E-4 0.0 1.4485080783415512 0.0 78-79 0.0 2.3931314516648363E-4 0.0 1.576660267578203 0.0 80-81 0.0 2.3931314516648363E-4 0.0 1.7132536841170911 0.0 82-83 0.0 2.6106888563616395E-4 0.0 1.8530234387645523 0.0 84-85 0.0 2.6106888563616395E-4 0.0 1.994196438672308 0.0 86-87 0.0 2.6106888563616395E-4 0.0 2.1433320395919666 0.0 88 0.0 2.6106888563616395E-4 0.0 2.2640763991986925 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4560 0.0 44.124462 1 GTATCAA 7365 0.0 34.596092 1 TCAACGC 8110 0.0 29.967228 4 ATCAACG 8135 0.0 29.875132 3 CAACGCA 8600 0.0 28.31476 5 AACGCAG 9210 0.0 26.4867 6 TATCAAC 9460 0.0 26.18764 2 GTACATG 12585 0.0 25.476023 1 TACATGG 12975 0.0 23.477102 2 ACGCAGA 10545 0.0 22.866037 7 CGCAGAG 10845 0.0 21.973225 8 ACATGGG 13540 0.0 21.941952 3 GAGTACA 9920 0.0 19.240435 1 GTGGTAT 2790 0.0 18.871841 1 CATGGGG 9815 0.0 18.822577 4 GCAGAGT 12250 0.0 18.762594 9 AGAGTAC 10640 0.0 17.714252 10-11 AGTACAT 10065 0.0 17.280838 2 CAGAGTA 11945 0.0 16.447893 10-11 GTATTAG 2350 0.0 16.403875 1 >>END_MODULE