FastQCFastQC Report
Wed 25 May 2016
SRR2049348_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049348_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4596488
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112330.24438223269591916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105890.23037153583344502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61350.13347146778148883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72550.054.040711
GTATCAA130300.042.2478751
ATCAACG159900.033.8578533
TCAACGC160500.033.731284
CAACGCA166250.032.5929035
TATCAAC169200.032.2468452
AACGCAG173600.031.3238186
ACGCAGA200150.027.0511867
CGCAGAG203200.026.3905098
GTGGTAT34450.026.3366491
GTACATG128750.024.6461451
GCAGAGT222500.023.6150999
TGGTATC36850.023.5933512
TACATGG129700.023.1897132
ACATGGG131500.021.978843
GAGTACT152550.021.03565812-13
CAGAGTA212950.020.79752510-11
AGAGTAC201200.020.58592410-11
GTACTTT167400.018.94996614-15
CATGGGG94600.018.6292574
Produced by FastQC (version 0.11.5)