##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049348_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4596488
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86549556966101	31.0	31.0	34.0	30.0	34.0
2	32.088909402134846	33.0	31.0	34.0	30.0	34.0
3	32.058402850176044	34.0	31.0	34.0	30.0	34.0
4	35.751673451556925	37.0	35.0	37.0	33.0	37.0
5	35.82232717674886	37.0	35.0	37.0	35.0	37.0
6	35.8526318354361	37.0	35.0	37.0	35.0	37.0
7	35.800569043147725	37.0	35.0	37.0	35.0	37.0
8	35.799669443279306	37.0	35.0	37.0	35.0	37.0
9	37.55240044138046	39.0	37.0	39.0	35.0	39.0
10-11	37.51247049921592	39.0	37.0	39.0	35.0	39.0
12-13	37.45714934967741	39.0	37.0	39.0	34.5	39.0
14-15	38.762393266337256	40.0	38.0	41.0	35.0	41.0
16-17	38.78759228785107	40.0	38.0	41.0	35.0	41.0
18-19	38.85458789406172	40.0	38.0	41.0	35.0	41.0
20-21	38.87954096692954	40.0	38.0	41.0	35.0	41.0
22-23	38.84851804247069	40.0	38.0	41.0	35.0	41.0
24-25	38.82879156869332	40.0	38.0	41.0	35.0	41.0
26-27	38.71580073743257	40.0	38.0	41.0	34.5	41.0
28-29	38.63859625000653	40.0	38.0	41.0	34.0	41.0
30-31	38.54360448672987	40.0	38.0	41.0	34.0	41.0
32-33	38.41313313555915	40.0	38.0	41.0	34.0	41.0
34-35	38.317061308546876	40.0	38.0	41.0	34.0	41.0
36-37	38.21331568797743	40.0	38.0	41.0	34.0	41.0
38-39	38.125837378450676	40.0	38.0	41.0	33.5	41.0
40-41	38.01888278616195	40.0	38.0	41.0	33.0	41.0
42-43	37.892066616947545	40.0	37.5	41.0	33.0	41.0
44-45	37.722303093144156	40.0	37.0	41.0	33.0	41.0
46-47	37.503476676105755	40.0	37.0	41.0	33.0	41.0
48-49	37.30412730328024	40.0	37.0	41.0	32.0	41.0
50-51	37.163077549642246	40.0	37.0	41.0	32.0	41.0
52-53	37.02323197623925	40.0	36.0	41.0	31.0	41.0
54-55	36.87831970843827	40.0	36.0	41.0	31.0	41.0
56-57	36.73597592335714	39.0	36.0	41.0	31.0	41.0
58-59	36.5439952198287	39.0	35.0	41.0	31.0	41.0
60-61	36.360860182817845	39.0	35.0	41.0	31.0	41.0
62-63	36.152824504273696	39.0	35.0	41.0	30.5	41.0
64-65	35.925169172637894	38.0	35.0	40.0	30.0	41.0
66-67	35.65665036001399	37.5	35.0	40.0	30.0	41.0
68-69	35.37176165803109	37.0	35.0	40.0	29.5	41.0
70-71	35.0474366516349	37.0	34.5	39.5	29.0	41.0
72-73	34.74952844432532	36.0	34.0	39.0	29.0	41.0
74-75	34.44585202876631	36.0	34.0	39.0	29.0	40.5
76-77	34.12301903105153	35.0	34.0	38.5	29.0	40.0
78-79	33.791470357368496	35.0	34.0	37.0	29.0	39.0
80-81	33.480135812385456	35.0	34.0	37.0	29.0	39.0
82-83	33.185898233607915	35.0	34.0	36.5	28.5	39.0
84-85	32.91861079589461	35.0	34.0	36.0	28.0	37.0
86-87	32.686691665462845	35.0	33.5	36.0	27.5	37.0
88-89	32.46741087978474	35.0	33.0	35.0	27.0	37.0
90-91	32.27956213526501	35.0	33.0	35.0	27.0	36.0
92-93	32.103766179744184	35.0	33.0	35.0	27.0	36.0
94-95	31.953318055001994	35.0	33.0	35.0	26.5	36.0
96-97	31.759079431948916	35.0	33.0	35.0	26.0	36.0
98-99	31.61484104820898	35.0	33.0	35.0	26.0	35.0
100	31.505233125812577	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.11051218514391437
1101	2	0.16236049208096404
1101	3	0.32801391690799875
1101	4	0.15026533561066913
1101	5	0.14228903824546535
1101	6	0.1819951070175847
1101	7	-0.03463737439754766
1101	8	0.052385936345594075
1101	9	0.16422073188788744
1101	10-11	0.18813813200970486
1101	12-13	0.1538943950163585
1101	14-15	0.2093132480278399
1101	16-17	0.13879956031062335
1101	18-19	0.2257076776654543
1101	20-21	0.18746903988038355
1101	22-23	0.18679571792219463
1101	24-25	0.22744919376343375
1101	26-27	0.26152584759906716
1101	28-29	0.2889417037155013
1101	30-31	0.31206785755318833
1101	32-33	0.3431552759878329
1101	34-35	0.30354082474033106
1101	36-37	0.33554618763540844
1101	38-39	0.2864062126645166
1101	40-41	0.23309776981921004
1101	42-43	0.2971824396915608
1101	44-45	0.3362761273086363
1101	46-47	0.364305277926114
1101	48-49	0.36310614147851794
1101	50-51	0.37961438504453326
1101	52-53	0.38553645259150926
1101	54-55	0.33286121536193036
1101	56-57	0.342156074447189
1101	58-59	0.3801661041239939
1101	60-61	0.38565144093714565
1101	62-63	0.3654872524384487
1101	64-65	0.3147761165292877
1101	66-67	0.2804189814866831
1101	68-69	0.3087568654406567
1101	70-71	0.2956448336359614
1101	72-73	0.2623131966915153
1101	74-75	0.31693372219186955
1101	76-77	0.23554024232535653
1101	78-79	0.2894541652613327
1101	80-81	0.3327396669103422
1101	82-83	0.3137582271594752
1101	84-85	0.31613025471547473
1101	86-87	0.21487200755768754
1101	88-89	0.2532711387813009
1101	90-91	0.29281984059570476
1101	92-93	0.3302742166100998
1101	94-95	0.3721955038121578
1101	96-97	0.38052272984318947
1101	98-99	0.39275201925308423
1101	100	0.45321868276312216
1102	1	0.25951368776604866
1102	2	0.2308505569563266
1102	3	0.3559695989963956
1102	4	0.22026808092228123
1102	5	0.21791754196076596
1102	6	0.14906314890585293
1102	7	0.180814546013238
1102	8	0.20514619455249772
1102	9	0.1385320098985403
1102	10-11	0.21122688720541305
1102	12-13	0.2077133642185487
1102	14-15	0.31222632144109497
1102	16-17	0.2639471781445266
1102	18-19	0.26724356018744544
1102	20-21	0.25315612087606354
1102	22-23	0.23265179332520347
1102	24-25	0.2560576062261717
1102	26-27	0.3489861530962983
1102	28-29	0.35211110043170635
1102	30-31	0.32217016656957753
1102	32-33	0.30847673530711006
1102	34-35	0.3121083925728243
1102	36-37	0.347323437088356
1102	38-39	0.2755422665222653
1102	40-41	0.313027839926761
1102	42-43	0.3693313988887681
1102	44-45	0.3675962654976246
1102	46-47	0.3062703310475783
1102	48-49	0.24493154762269853
1102	50-51	0.3505091808431473
1102	52-53	0.4031456440372807
1102	54-55	0.3774375345707739
1102	56-57	0.39353194681586956
1102	58-59	0.36200666291679084
1102	60-61	0.3328468353887857
1102	62-63	0.3624612978466146
1102	64-65	0.3502239894040571
1102	66-67	0.33445047892576696
1102	68-69	0.2901509705807115
1102	70-71	0.3024409874616083
1102	72-73	0.3999400730570528
1102	74-75	0.35021492696806433
1102	76-77	0.3449924473023174
1102	78-79	0.37162065966790436
1102	80-81	0.40764529987953324
1102	82-83	0.39671531124040627
1102	84-85	0.3845884799365962
1102	86-87	0.3467014125761807
1102	88-89	0.3561565402171709
1102	90-91	0.4295152178993291
1102	92-93	0.490891989255978
1102	94-95	0.430340905769107
1102	96-97	0.4614967215139423
1102	98-99	0.4478119899112336
1102	100	0.4614489132424069
1103	1	0.1369352628909617
1103	2	0.1562061268705648
1103	3	0.309601858210641
1103	4	0.15365617747664118
1103	5	0.16418659783359857
1103	6	0.09652227400940205
1103	7	0.15083552021086177
1103	8	0.15415898954266538
1103	9	0.21697813741656802
1103	10-11	0.2510697856613362
1103	12-13	0.1573748994437807
1103	14-15	0.2813038661754561
1103	16-17	0.24387129458054346
1103	18-19	0.24632837849678424
1103	20-21	0.2696688585794931
1103	22-23	0.2694852470666689
1103	24-25	0.2524166351884034
1103	26-27	0.31092542442372206
1103	28-29	0.3094388618571955
1103	30-31	0.3636765670321864
1103	32-33	0.37768568588970197
1103	34-35	0.37132219629598495
1103	36-37	0.31612290407513655
1103	38-39	0.36355474380810193
1103	40-41	0.3897665437461981
1103	42-43	0.4524004825002095
1103	44-45	0.4470009948185165
1103	46-47	0.39825661141487245
1103	48-49	0.26729134800915233
1103	50-51	0.34639149712658934
1103	52-53	0.41520159623424036
1103	54-55	0.3680646990695706
1103	56-57	0.3527029355354401
1103	58-59	0.4566392001195396
1103	60-61	0.45229732627086605
1103	62-63	0.38060988150537156
1103	64-65	0.4093939718405153
1103	66-67	0.4289749020527722
1103	68-69	0.4074364690080188
1103	70-71	0.4339272156293674
1103	72-73	0.4392823958677212
1103	74-75	0.37449853539041555
1103	76-77	0.3427197312364356
1103	78-79	0.3554689790222767
1103	80-81	0.4347002700021818
1103	82-83	0.43006225059239256
1103	84-85	0.37819305989472696
1103	86-87	0.4231179607702984
1103	88-89	0.45531902484040643
1103	90-91	0.43929191653702304
1103	92-93	0.49628806530071756
1103	94-95	0.4338534585708018
1103	96-97	0.48709204563092
1103	98-99	0.5339482177521155
1103	100	0.5658467921404089
1104	1	0.22233148761772625
1104	2	0.20793312775650463
1104	3	0.29083281844191333
1104	4	0.19080990661282016
1104	5	0.1778058355320553
1104	6	0.14810306302672416
1104	7	0.16434481482021113
1104	8	0.1429782535279429
1104	9	0.15544123598172632
1104	10-11	0.17468419710269956
1104	12-13	0.21126747462193407
1104	14-15	0.29934392706925905
1104	16-17	0.29132985047571225
1104	18-19	0.27649501627282547
1104	20-21	0.2794224578000808
1104	22-23	0.2789478488503647
1104	24-25	0.3049022133618706
1104	26-27	0.2869137872716081
1104	28-29	0.3496188892687613
1104	30-31	0.31191239654550174
1104	32-33	0.3558042872437426
1104	34-35	0.29849426186324024
1104	36-37	0.37442132093325853
1104	38-39	0.311156652536809
1104	40-41	0.26504354091946425
1104	42-43	0.31877175700914506
1104	44-45	0.252937511971588
1104	46-47	0.23850419827559932
1104	48-49	0.24496680965108908
1104	50-51	0.21153988533644963
1104	52-53	0.16609508222846614
1104	54-55	0.1698772243542237
1104	56-57	0.21111970100289312
1104	58-59	0.35048871861557274
1104	60-61	0.34622404729309153
1104	62-63	0.2732754527733974
1104	64-65	0.23400664765669177
1104	66-67	0.23096666931865428
1104	68-69	0.17668955006335807
1104	70-71	0.2411312204576035
1104	72-73	0.20512580413608106
1104	74-75	0.17208573713975994
1104	76-77	0.08855847325070698
1104	78-79	0.1352419150145039
1104	80-81	0.10824730215863099
1104	82-83	0.17543462457511083
1104	84-85	0.146995001087312
1104	86-87	0.10189717724541936
1104	88-89	0.10484229092999442
1104	90-91	0.10014738530059475
1104	92-93	0.23139130220456394
1104	94-95	0.22319966867208763
1104	96-97	0.3348440848006895
1104	98-99	0.35576476584711614
1104	100	0.3580165192185909
1105	1	0.13802877198020624
1105	2	0.12747844591577007
1105	3	0.22371118492259257
1105	4	0.12386967821138484
1105	5	0.07888492878485209
1105	6	0.11355151913826944
1105	7	0.127903078486149
1105	8	0.10046967800386852
1105	9	0.15696078864888818
1105	10-11	0.14201288267037881
1105	12-13	0.11129977044971895
1105	14-15	0.183277026154272
1105	16-17	0.17394927451052666
1105	18-19	0.1497928830207158
1105	20-21	0.11959785861156291
1105	22-23	0.12758689206201979
1105	24-25	0.1131497541708768
1105	26-27	0.12854263496881657
1105	28-29	0.11746090492278682
1105	30-31	0.1526820140756584
1105	32-33	0.18575476103670496
1105	34-35	0.20815091943314457
1105	36-37	0.1932597784537009
1105	38-39	0.18963987210771194
1105	40-41	0.24777211872115856
1105	42-43	0.2947954603902758
1105	44-45	0.22263458240942668
1105	46-47	0.2281806977293357
1105	48-49	0.15769841653298045
1105	50-51	0.17720655370158767
1105	52-53	0.2361047709768016
1105	54-55	0.167172531628772
1105	56-57	0.21426885490895842
1105	58-59	0.23047893872969638
1105	60-61	0.19521382501734053
1105	62-63	0.21014612998036597
1105	64-65	0.1984136710298685
1105	66-67	0.19745144203883314
1105	68-69	0.21287304612482671
1105	70-71	0.21734169364325595
1105	72-73	0.24163304557237808
1105	74-75	0.25495585360234685
1105	76-77	0.18329781389799393
1105	78-79	0.13491967133767702
1105	80-81	0.18420509238993077
1105	82-83	0.1835771728741733
1105	84-85	0.1546252321694226
1105	86-87	0.1368278325603285
1105	88-89	0.1528981595636978
1105	90-91	0.13062760843509835
1105	92-93	0.2209043547771259
1105	94-95	0.24553358408427783
1105	96-97	0.24297487221531
1105	98-99	0.21481812608590545
1105	100	0.2224338204199796
1106	1	0.10740900134616993
1106	2	0.15251636251312561
1106	3	0.2182645396073184
1106	4	0.16115063042351352
1106	5	0.1383232352413657
1106	6	0.10112216614562897
1106	7	0.11683832461917376
1106	8	0.10563749787174004
1106	9	0.16093489207482747
1106	10-11	0.17493688698096577
1106	12-13	0.12786374968924008
1106	14-15	0.18904878098030053
1106	16-17	0.1871714222596168
1106	18-19	0.2025943911124557
1106	20-21	0.17636399110502055
1106	22-23	0.15041178181355974
1106	24-25	0.15619379295667812
1106	26-27	0.20035140835725684
1106	28-29	0.23920718982093803
1106	30-31	0.24962328410354928
1106	32-33	0.1436828477337997
1106	34-35	0.14588249865118996
1106	36-37	0.15427429349231403
1106	38-39	0.14819563636640964
1106	40-41	0.21202858015756476
1106	42-43	0.22737714680737753
1106	44-45	0.18526969028482654
1106	46-47	0.14381107616220845
1106	48-49	0.11422792175254415
1106	50-51	0.10968253948957596
1106	52-53	0.1115281915816837
1106	54-55	0.1220405250558727
1106	56-57	0.09685982916827385
1106	58-59	0.12471883299382824
1106	60-61	0.022212023842591577
1106	62-63	0.07307195262203692
1106	64-65	0.04765792854790618
1106	66-67	0.0553839909840832
1106	68-69	0.10847376592208491
1106	70-71	0.13322863633042914
1106	72-73	0.1628610630695917
1106	74-75	0.13606260485166644
1106	76-77	0.12988576455877876
1106	78-79	0.12343563830854976
1106	80-81	0.10351332221088683
1106	82-83	0.15031789938061735
1106	84-85	0.12567317923599575
1106	86-87	0.15152570522906217
1106	88-89	0.1529767844161256
1106	90-91	0.14686109303710992
1106	92-93	0.17684294743611417
1106	94-95	0.09091832726748805
1106	96-97	0.16108706338354395
1106	98-99	0.2314655247423758
1106	100	0.22466800741067772
1107	1	0.11975954355509799
1107	2	0.098462913449751
1107	3	0.19073665187791988
1107	4	0.11665130924161105
1107	5	0.08576581094965974
1107	6	0.08887695541225327
1107	7	0.05217996369935207
1107	8	0.05611595074795872
1107	9	0.05648248018415103
1107	10-11	0.09516592143641134
1107	12-13	0.04932327315151497
1107	14-15	0.1474089768807545
1107	16-17	0.10050188266666282
1107	18-19	0.13053686188164448
1107	20-21	0.08261970375338024
1107	22-23	0.11331190360099441
1107	24-25	0.11904626152114872
1107	26-27	0.180751308161895
1107	28-29	0.1834359784179398
1107	30-31	0.1681914301153853
1107	32-33	0.16700177288269913
1107	34-35	0.14473408344358063
1107	36-37	0.23531941872062845
1107	38-39	0.16719600044565652
1107	40-41	0.19101245924291987
1107	42-43	0.2699655815957769
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2216	64-65	-0.5824788405810608
2216	66-67	-0.4660587173939774
2216	68-69	-0.4788359137886218
2216	70-71	-0.5526716204210729
2216	72-73	-0.5685513918536671
2216	74-75	-0.5791347680952441
2216	76-77	-0.47963511674669235
2216	78-79	-0.6819406854271008
2216	80-81	-0.7731706152917468
2216	82-83	-0.6661027303952736
2216	84-85	-0.7308408038179337
2216	86-87	-0.6272835462707462
2216	88-89	-0.9284370844755756
2216	90-91	-0.7124836256685896
2216	92-93	-0.7539217731924381
2216	94-95	-0.6771214075978449
2216	96-97	-0.6670879927289413
2216	98-99	-0.7951164620800206
2216	100	-0.780120869414656
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	277.0
3	25.0
4	50.0
5	82.0
6	192.0
7	530.0
8	1447.0
9	2443.0
10	3242.0
11	4661.0
12	6506.0
13	9312.0
14	11099.0
15	14092.0
16	17509.0
17	23931.0
18	24503.0
19	13860.0
20	10965.0
21	11001.0
22	11489.0
23	12686.0
24	15154.0
25	18724.0
26	24144.0
27	31665.0
28	41567.0
29	55024.0
30	71174.0
31	92465.0
32	119744.0
33	155450.0
34	212704.0
35	310130.0
36	506471.0
37	926756.0
38	1302650.0
39	530512.0
40	2252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.94599825143662	20.190139399720845	14.41391677108206	23.449945577760467
2	15.530552891686	20.36859445733351	39.91445207732512	24.186400573655366
3	18.032114953851725	25.087914947237977	30.74712693691357	26.13284316199673
4	12.55511002206685	16.914199909626635	38.0350254444318	32.49566462387471
5	12.725084890899312	37.72756504531286	35.688530025532536	13.858820038255292
6	31.145779125279997	37.89813005059515	17.048320369812778	13.907770454312073
7	26.738653511115444	32.91119219717314	22.20075414098764	18.14940015072377
8	25.05943169657125	36.44821343762244	20.02646388421681	18.4658909815895
9	24.770890297113795	16.44594742768827	21.451312393288095	37.33184988190984
10-11	23.999953877830205	26.911350578963766	28.534307062261448	20.55438848094458
12-13	24.483602138309283	24.400730791422994	29.019684747166757	22.095982323100962
14-15	21.707975238027068	26.330922323554518	26.60327182725664	25.35783061116177
16-17	20.904192701948247	29.757878136063475	27.327413304987047	22.010515857001227
18-19	20.802880722360516	28.15505148933337	30.115037878936214	20.927029909369896
20-21	21.768564072641578	27.35837768242958	30.056112430234748	20.816945814694094
22-23	21.46175514871354	27.16459827590108	30.125761233359032	21.247885342026347
24-25	21.328097006426006	27.308685626541536	30.059106111438776	21.304111255593682
26-27	21.103691190115924	27.32905687715722	30.120711804781266	21.446540127945593
28-29	21.112661093835765	27.327292896914084	30.268845615556472	21.29120039369368
30-31	21.36614954721953	27.58587643435597	29.8661391044641	21.181834913960397
32-33	21.146701264151996	27.42922546935639	30.160075736207297	21.26399753028431
34-35	21.283992006935733	27.244785961446627	30.115077064321977	21.356144967295663
36-37	21.535974185483315	27.49519279249017	29.673521073229132	21.29531194879738
38-39	21.401758915758794	27.628083128175806	29.72120114836	21.248956807705394
40-41	21.294733104694803	27.426800571915404	29.833281264549168	21.445185058840625
42-43	21.306908635617756	27.52594797188922	29.684436329499402	21.48270706299362
44-45	21.43219688151995	27.604096186856435	29.50593607703303	21.45777085459059
46-47	21.532239221213093	27.73064269587045	29.15168600682	21.58543207609646
48-49	21.582709944020877	27.74650542468088	28.913385353965364	21.757399277332887
50-51	21.62827568839699	27.805097730154145	28.736467159763794	21.830159421685067
52-53	21.60020058936724	27.88645815090107	28.729836363023242	21.783504896708443
54-55	21.678843971355786	28.108312907290294	28.593218629856604	21.619624491497316
56-57	21.571191447669065	27.916375545542767	28.65071328346664	21.861719723321528
58-59	21.491204787665687	27.649495983664014	28.903282034636085	21.956017194034214
60-61	21.675785607107358	27.887455979873284	28.68751437242044	21.74924404059892
62-63	21.499110614494025	27.964125217124934	28.687458010909335	21.84930615747171
64-65	21.455028869465025	27.759764540819464	28.829989042667677	21.955217547047834
66-67	21.46480856416296	27.734495805911113	28.688631854929376	22.11206377499655
68-69	21.699458186080793	27.833122879324137	28.596794630246375	21.870624304348695
70-71	21.721757426176076	27.76598428290638	28.600789672836306	21.91146861808124
72-73	21.588853517215274	27.87396298538376	28.622838062821614	21.914345434579356
74-75	21.649989970485816	27.921068811169704	28.474149743039927	21.954791475304553
76-77	21.698542374142463	27.864304755390414	28.367236693907273	22.06991617655985
78-79	21.682900281028743	27.932357728246927	28.427252891276012	21.95748909944832
80-81	21.631401673474038	27.831723166549054	28.489916981163272	22.046958178813636
82-83	21.568153298036393	27.886782617473727	28.549622837888172	21.995441246601715
84-85	21.643413274585328	27.815097812842765	28.386898791205073	22.154590121366834
86-87	21.669262343096825	27.951002360421157	28.33778117365331	22.04195412282871
88-89	21.518156475173292	27.824006685245738	28.42539161979384	22.232445219787138
90-91	21.44895327327484	27.723994188870414	28.474404432645233	22.35264810520951
92-93	21.51265722058621	27.917311242560295	28.351360427259042	22.218671109594453
94-95	21.412930781562565	27.72493770276449	28.48679859926813	22.375332916404815
96-97	21.451452203888678	27.768215220735883	28.431536255519745	22.348796319855694
98-99	21.389664050137114	27.811523716997087	28.41892886657701	22.37988336628879
100	21.475739992294024	27.688644651189303	28.356662655585318	22.478952700931355
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	660.0
1	495.5
2	597.0
3	863.0
4	553.5
5	352.5
6	905.0
7	2397.0
8	4141.0
9	4690.5
10	3542.0
11	2250.0
12	2073.0
13	2348.5
14	2661.0
15	3047.0
16	3526.5
17	4092.5
18	4777.5
19	5617.0
20	6913.0
21	8731.0
22	11016.0
23	14428.0
24	19240.0
25	25384.5
26	33350.0
27	42816.5
28	54518.0
29	68153.5
30	81515.0
31	93814.5
32	103919.0
33	113040.5
34	123126.5
35	131885.0
36	139447.0
37	147636.5
38	154869.0
39	158654.5
40	161009.0
41	163345.0
42	163956.5
43	163791.0
44	162025.0
45	158963.5
46	155492.5
47	150493.0
48	144953.0
49	141089.5
50	138235.0
51	133695.5
52	126099.0
53	119160.0
54	113448.0
55	107406.0
56	100163.0
57	90963.0
58	83226.5
59	76519.5
60	67740.5
61	57738.0
62	49007.0
63	40950.0
64	33212.0
65	26858.0
66	21518.5
67	16579.5
68	12752.0
69	9839.0
70	7232.5
71	5302.0
72	3873.5
73	2699.0
74	1973.5
75	1339.5
76	814.5
77	543.0
78	334.5
79	183.5
80	112.5
81	62.0
82	28.5
83	14.0
84	9.5
85	7.0
86	3.5
87	2.5
88	1.5
89	2.0
90	1.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04044392153313573
2	0.0
3	0.0
4	2.175574046968033E-5
5	0.0
6	0.0
7	0.0
8	7.614509164388116E-4
9	0.0
10-11	0.0
12-13	0.04388132852734523
14-15	9.79008321135615E-5
16-17	2.175574046968033E-5
18-19	4.351148093936066E-5
20-21	4.351148093936066E-5
22-23	0.0
24-25	1.95801664227123E-4
26-27	6.526722140904099E-5
28-29	4.568705498632869E-4
30-31	0.0
32-33	1.6316805352260247E-4
34-35	6.526722140904099E-5
36-37	9.79008321135615E-5
38-39	4.3511480939360656E-4
40-41	8.702296187872132E-5
42-43	6.635500843252501E-4
44-45	3.91603328454246E-4
46-47	1.3053444281808197E-4
48-49	9.24618969961414E-4
50-51	6.635500843252501E-4
52-53	7.179394354994509E-4
54-55	5.982828629162091E-4
56-57	7.288173057342911E-4
58-59	2.1755740469680328E-4
60-61	2.3931314516648366E-4
62-63	0.0012183214663020984
64-65	4.895041605678074E-4
66-67	0.0017078256268699059
68-69	0.0016534362756957049
70-71	5.982828629162091E-4
72-73	0.00131622229841566
74-75	0.0011748099853627378
76-77	8.919853592568936E-4
78-79	0.003502674215618533
80-81	0.00264332246706616
82-83	0.0012183214663020984
84-85	0.002904391352702324
86-87	0.0013597337793550207
88-89	0.002121184695793832
90-91	0.002947902833641685
92-93	0.0025998109861267997
94-95	0.0038398881928985783
96-97	0.003187215978808168
98-99	0.0028826356122326436
100	0.0011965657258324182
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4596488.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.584160567623016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.87750981279429	47.34436908350138
2	13.887419213453542	17.104901094224317
3	4.216909404236216	7.79084477548808
4	1.7625589454577584	4.341828524278836
5	0.8929711616518659	2.7496439700712676
6	0.5464653746905151	2.0192166827752174
7	0.37352564021416523	1.6102284102151336
8	0.24659241734090084	1.2148949619424276
9	0.17661281029107298	0.9788896500538123
>10	0.9568534522938793	10.395146771394645
>50	0.0464905157426595	1.9624485556273965
>100	0.015349584201916743	1.5081641981120826
>500	4.944452443332748E-4	0.22567790598568876
>1k	1.4126993527788887E-4	0.1456813388714807
>5k	3.5317483819282564E-5	0.13343610241466852
>10k+	7.063496763856513E-5	0.47462797504366694
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11233	0.24438223269591916	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10589	0.23037153583344502	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6135	0.13347146778148883	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.175574046968033E-5	0.0
4	0.0	0.0	0.0	2.175574046968033E-5	0.0
5	0.0	0.0	0.0	2.175574046968033E-5	0.0
6	0.0	0.0	0.0	2.175574046968033E-5	0.0
7	0.0	0.0	0.0	4.351148093936066E-5	0.0
8	0.0	0.0	0.0	4.351148093936066E-5	0.0
9	0.0	0.0	0.0	8.702296187872132E-5	0.0
10-11	0.0	0.0	0.0	8.702296187872132E-5	0.0
12-13	0.0	0.0	0.0	9.790083211356148E-5	0.0
14-15	0.0	0.0	0.0	1.1965657258324181E-4	0.0
16-17	0.0	0.0	0.0	1.6316805352260247E-4	0.0
18-19	0.0	2.175574046968033E-5	0.0	2.175574046968033E-4	0.0
20-21	0.0	2.175574046968033E-5	0.0	2.3931314516648363E-4	0.0
22-23	0.0	2.175574046968033E-5	0.0	3.5896971774972543E-4	0.0
24-25	0.0	2.175574046968033E-5	0.0	5.438935117420083E-4	0.0
26-27	0.0	4.351148093936066E-5	0.0	8.919853592568935E-4	0.0
28-29	0.0	6.526722140904099E-5	0.0	0.002382253581429996	0.0
30-31	0.0	6.526722140904099E-5	0.0	0.006972714820532546	0.0
32-33	0.0	6.526722140904099E-5	0.0	0.01849237939922828	0.0
34-35	0.0	6.526722140904099E-5	0.0	0.036136284920139025	0.0
36-37	0.0	6.526722140904099E-5	0.0	0.06298286865972455	0.0
38-39	0.0	1.1965657258324181E-4	0.0	0.09785732063262212	0.0
40-41	0.0	1.3053444281808197E-4	0.0	0.1331342538042088	0.0
42-43	0.0	1.3053444281808197E-4	0.0	0.16998847815984724	0.0
44-45	0.0	1.3053444281808197E-4	0.0	0.21332591317545047	0.0
46-47	0.0	1.5229018328776232E-4	0.0	0.2649196516992974	0.0
48-49	0.0	1.5229018328776232E-4	0.0	0.31901530037715753	0.0
50-51	0.0	1.5229018328776232E-4	0.0	0.3707178176033528	0.0
52-53	0.0	1.5229018328776232E-4	0.0	0.42269228158541916	0.0
54-55	0.0	1.5229018328776232E-4	0.0	0.48105205539533663	0.0
56-57	0.0	1.9580166422712296E-4	0.0	0.5485057287215804	0.0
58-59	0.0	2.175574046968033E-4	0.0	0.6223447118756755	0.0
60-61	0.0	2.175574046968033E-4	0.0	0.6965209090070505	0.0
62-63	0.0	2.2843527493164345E-4	0.0	0.7678471041368975	0.0
64-65	0.0	2.3931314516648363E-4	0.0	0.8456238763160047	0.0
66-67	0.0	2.501910154013238E-4	0.0	0.9333647776302255	0.0
68-69	0.0	2.6106888563616395E-4	0.0	1.0283720962613194	0.0
70-71	0.0	2.6106888563616395E-4	0.0	1.131853275805354	0.0
72-73	0.0	2.6106888563616395E-4	0.0	1.2355302570136155	0.0
74-75	0.0	2.7194675587100415E-4	0.0	1.3410999876427394	0.0
76-77	0.0	3.2633610704520494E-4	0.0	1.4555678161239625	0.0
78-79	0.0	3.2633610704520494E-4	0.0	1.5847534030329242	0.0
80-81	0.0	3.2633610704520494E-4	0.0	1.7225216295571748	0.0
82-83	0.0	3.2633610704520494E-4	0.0	1.8634226827090594	0.0
84-85	0.0	3.2633610704520494E-4	0.0	2.0056617138998294	0.0
86-87	0.0	3.2633610704520494E-4	0.0	2.1557980788810935	0.0
88	0.0	3.2633610704520494E-4	0.0	2.2779783173588184	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7255	0.0	54.04071	1
GTATCAA	13030	0.0	42.247875	1
ATCAACG	15990	0.0	33.857853	3
TCAACGC	16050	0.0	33.73128	4
CAACGCA	16625	0.0	32.592903	5
TATCAAC	16920	0.0	32.246845	2
AACGCAG	17360	0.0	31.323818	6
ACGCAGA	20015	0.0	27.051186	7
CGCAGAG	20320	0.0	26.390509	8
GTGGTAT	3445	0.0	26.336649	1
GTACATG	12875	0.0	24.646145	1
GCAGAGT	22250	0.0	23.615099	9
TGGTATC	3685	0.0	23.593351	2
TACATGG	12970	0.0	23.189713	2
ACATGGG	13150	0.0	21.97884	3
GAGTACT	15255	0.0	21.035658	12-13
CAGAGTA	21295	0.0	20.797525	10-11
AGAGTAC	20120	0.0	20.585924	10-11
GTACTTT	16740	0.0	18.949966	14-15
CATGGGG	9460	0.0	18.629257	4
>>END_MODULE