FastQCFastQC Report
Wed 25 May 2016
SRR2049347_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049347_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4935310
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49800.046.534171
GTATCAA82250.034.347331
TCAACGC88250.030.3608914
ATCAACG89200.029.9321463
CAACGCA93450.028.6714695
AACGCAG98200.027.5208826
TATCAAC103500.026.386742
ACGCAGA114950.023.1833217
GCCGTAT10400.023.04780694
CGCAGAG115100.022.7447648
GTACATG122200.022.1951961
GTGGTAT30300.020.4779131
TACATGG122750.020.2957132
ACATGGG126100.019.3094123
GCAGAGT130250.019.2333749
TGGTATC31050.017.8636572
CAGAGTA126800.016.93958910-11
AGAGTAC112800.016.79197910-11
CATGGGG90150.015.7990624
CGCCGTA10450.015.74144292-93
Produced by FastQC (version 0.11.5)