FastQCFastQC Report
Wed 25 May 2016
SRR2049347_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049347_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4935310
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT133680.27086444417878514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112630.22821261481041719No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107430.21767629591656856No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT66660.13506750335845163RNA PCR Primer, Index 46 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59050.11964800590033857No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82500.058.2941361
GTATCAA150400.040.5723421
GTGGTAT33250.035.4883041
ATCAACG189300.031.9017773
TCAACGC189850.031.8588684
TGGTATC35450.031.0214422
CAACGCA196500.030.8046075
TATCAAC197700.030.7127172
AACGCAG204700.029.6199846
ACGCAGA230550.025.9724567
CGCAGAG231750.025.716198
GCAGAGT253450.023.1432259
GAGTACT176000.021.5984912-13
GTACATG125800.020.6282141
CAGAGTA249100.020.50717410-11
AGAGTAC237050.020.49793210-11
AGTACTT186050.019.52202412-13
GTACTTT196500.019.32462914-15
TACATGG126750.019.0950872
ACATGGG129200.017.8599973
Produced by FastQC (version 0.11.5)