##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049347_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4935310 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.733265590206088 31.0 31.0 34.0 30.0 34.0 2 31.81062851168417 31.0 31.0 34.0 30.0 34.0 3 31.807812072595237 33.0 31.0 34.0 30.0 34.0 4 35.584451635256954 37.0 35.0 37.0 33.0 37.0 5 35.708675848123015 37.0 35.0 37.0 33.0 37.0 6 35.78702330755312 37.0 35.0 37.0 35.0 37.0 7 35.72002670551596 37.0 35.0 37.0 33.0 37.0 8 35.72041411785683 37.0 35.0 37.0 33.0 37.0 9 37.43631727287647 39.0 37.0 39.0 34.0 39.0 10-11 37.40972400923144 39.0 37.0 39.0 34.0 39.0 12-13 37.365783608324506 39.0 37.0 39.0 34.0 39.0 14-15 38.63301494738933 40.0 38.0 41.0 34.0 41.0 16-17 38.66175498600899 40.0 38.0 41.0 34.0 41.0 18-19 38.733116156837156 40.0 38.0 41.0 34.0 41.0 20-21 38.76962359000751 40.0 38.0 41.0 34.0 41.0 22-23 38.73751405686775 40.0 38.0 41.0 34.0 41.0 24-25 38.71897094610065 40.0 38.0 41.0 34.0 41.0 26-27 38.6043330611451 40.0 38.0 41.0 34.0 41.0 28-29 38.5148570201264 40.0 38.0 41.0 34.0 41.0 30-31 38.4075836168346 40.0 38.0 41.0 34.0 41.0 32-33 38.26487687703508 40.0 38.0 41.0 34.0 41.0 34-35 38.15397219222298 40.0 38.0 41.0 33.5 41.0 36-37 38.0242647371695 40.0 38.0 41.0 33.0 41.0 38-39 37.91483706190695 40.0 37.0 41.0 33.0 41.0 40-41 37.816655995266764 40.0 37.0 41.0 33.0 41.0 42-43 37.68180762707915 40.0 37.0 41.0 33.0 41.0 44-45 37.508409907381704 40.0 37.0 41.0 33.0 41.0 46-47 37.27856142775226 40.0 37.0 41.0 32.0 41.0 48-49 37.06907529618201 40.0 37.0 41.0 31.0 41.0 50-51 36.908561265655045 40.0 36.5 41.0 31.0 41.0 52-53 36.78064640316414 40.0 36.0 41.0 31.0 41.0 54-55 36.646086466706244 39.5 36.0 41.0 31.0 41.0 56-57 36.50355702073426 39.0 35.5 41.0 31.0 41.0 58-59 36.319666140526124 39.0 35.0 41.0 30.0 41.0 60-61 36.13572926523359 39.0 35.0 41.0 30.0 41.0 62-63 35.9353957502163 39.0 35.0 41.0 30.0 41.0 64-65 35.68828503174066 38.0 35.0 40.0 29.0 41.0 66-67 35.38789397221248 38.0 35.0 40.0 29.0 41.0 68-69 35.051864624511936 37.0 34.5 40.0 29.0 41.0 70-71 34.68665575212094 37.0 34.0 39.5 28.0 41.0 72-73 34.38150217514199 36.0 34.0 39.0 28.0 41.0 74-75 34.0659380059206 36.0 34.0 39.0 28.0 40.5 76-77 33.72307565279587 35.0 34.0 38.5 27.5 40.0 78-79 33.3689974895194 35.0 34.0 37.0 27.0 39.0 80-81 33.03113208694084 35.0 33.0 37.0 26.0 39.0 82-83 32.71783738002273 35.0 33.0 36.5 26.0 39.0 84-85 32.43551408118233 35.0 33.0 36.0 26.0 37.0 86-87 32.188904344407945 35.0 33.0 36.0 26.0 37.0 88-89 31.938380669096773 35.0 33.0 35.0 25.5 37.0 90-91 31.724089064314096 35.0 33.0 35.0 25.0 36.0 92-93 31.527065574401608 35.0 33.0 35.0 25.0 36.0 94-95 31.35442879981197 35.0 33.0 35.0 24.5 36.0 96-97 31.138968575428898 35.0 33.0 35.0 23.5 36.0 98-99 30.962890274369798 35.0 32.5 35.0 23.0 35.0 100 30.828775700006688 35.0 32.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21459745049594403 1101 2 0.22537314141044007 1101 3 0.42540799232736504 1101 4 0.22479564829088616 1101 5 0.2464794606137488 1101 6 0.23613753625051004 1101 7 0.11155606297141674 1101 8 0.14514173608002068 1101 9 0.24179678414900252 1101 10-11 0.26470136366744157 1101 12-13 0.1759573126145142 1101 14-15 0.33241646807356773 1101 16-17 0.28068616791502166 1101 18-19 0.3935024189192333 1101 20-21 0.35523194962844684 1101 22-23 0.31093068197596807 1101 24-25 0.3395930616023861 1101 26-27 0.3839783214687742 1101 28-29 0.36326511117086113 1101 30-31 0.33559033755334156 1101 32-33 0.37488986618660647 1101 34-35 0.37555869311383105 1101 36-37 0.3929804989711698 1101 38-39 0.37694648582812107 1101 40-41 0.3340393545846254 1101 42-43 0.4129497079840476 1101 44-45 0.41633860573156056 1101 46-47 0.4874884788612732 1101 48-49 0.5295711907821996 1101 50-51 0.4742719855652098 1101 52-53 0.4837207993460524 1101 54-55 0.43350474162059527 1101 56-57 0.44537703616179414 1101 58-59 0.45377143723120383 1101 60-61 0.4819198969993508 1101 62-63 0.45320106481705835 1101 64-65 0.42814812987626993 1101 66-67 0.4239589411530744 1101 68-69 0.4553246987705748 1101 70-71 0.45566210440093613 1101 72-73 0.3281800056871731 1101 74-75 0.43339960422228785 1101 76-77 0.40172863101204825 1101 78-79 0.41984648008502745 1101 80-81 0.4640489992158905 1101 82-83 0.41959258948592293 1101 84-85 0.4308881854891027 1101 86-87 0.42571281626783275 1101 88-89 0.47871543427122987 1101 90-91 0.4903695437278017 1101 92-93 0.502727151817826 1101 94-95 0.5274782722771469 1101 96-97 0.5170027168668945 1101 98-99 0.5922193536520766 1101 100 0.5847752196438414 1102 1 0.30014831329763325 1102 2 0.2511546959283457 1102 3 0.4756151023158637 1102 4 0.24219164313188912 1102 5 0.2613182118470121 1102 6 0.23218880631154093 1102 7 0.19443637939143343 1102 8 0.2571803931052372 1102 9 0.21759372967296997 1102 10-11 0.2597574933272 1102 12-13 0.2641440468734757 1102 14-15 0.36238930597819063 1102 16-17 0.3185948710623734 1102 18-19 0.30919619777817076 1102 20-21 0.2934407640327166 1102 22-23 0.3206040131901844 1102 24-25 0.38464075875664605 1102 26-27 0.42979593234981195 1102 28-29 0.41032440287588656 1102 30-31 0.3380709735213827 1102 32-33 0.3989281720159781 1102 34-35 0.4112149584932183 1102 36-37 0.39218620377011604 1102 38-39 0.3792583327298189 1102 40-41 0.356142684191866 1102 42-43 0.4003328241476751 1102 44-45 0.45652563526183343 1102 46-47 0.4266217263225016 1102 48-49 0.41150946518394704 1102 50-51 0.4863338140765947 1102 52-53 0.551537747127945 1102 54-55 0.4241075698844341 1102 56-57 0.5241073819518078 1102 58-59 0.5184087243745736 1102 60-61 0.45378616620558887 1102 62-63 0.5334523827688216 1102 64-65 0.44989613770887615 1102 66-67 0.43602017108375435 1102 68-69 0.5262421983391192 1102 70-71 0.4889871331397657 1102 72-73 0.49882599760475443 1102 74-75 0.3683374102987713 1102 76-77 0.41070661455147217 1102 78-79 0.4785890514581297 1102 80-81 0.4318508841422144 1102 82-83 0.43857107134376605 1102 84-85 0.3929674572831985 1102 86-87 0.4188557537670974 1102 88-89 0.4173136147270142 1102 90-91 0.5154942735140509 1102 92-93 0.481247682248398 1102 94-95 0.5020519647112955 1102 96-97 0.5008590704129396 1102 98-99 0.5574794618681977 1102 100 0.5858196146849508 1103 1 0.17471712222050684 1103 2 0.1526759108278597 1103 3 0.3158943683163997 1103 4 0.17376219977949603 1103 5 0.18663721760319163 1103 6 0.12053628063102195 1103 7 0.12230809096781314 1103 8 0.1596230406569532 1103 9 0.20274755108462728 1103 10-11 0.19619687509445072 1103 12-13 0.17801550316308834 1103 14-15 0.27786129335495247 1103 16-17 0.2573279176598575 1103 18-19 0.26154793559319245 1103 20-21 0.20717364821729944 1103 22-23 0.25914901499231036 1103 24-25 0.2752148785510684 1103 26-27 0.27833221971309996 1103 28-29 0.36617446401295695 1103 30-31 0.2972247154772134 1103 32-33 0.3337243197397939 1103 34-35 0.2941020199609241 1103 36-37 0.3357864665369874 1103 38-39 0.3196298269967244 1103 40-41 0.3975697631564472 1103 42-43 0.43729686738824114 1103 44-45 0.3987073199881763 1103 46-47 0.3467395524813597 1103 48-49 0.37154183564386756 1103 50-51 0.42974961423922764 1103 52-53 0.3707122710542379 1103 54-55 0.34934679599434304 1103 56-57 0.39042613151048755 1103 58-59 0.46011096216531655 1103 60-61 0.42408688750708734 1103 62-63 0.3942662051855166 1103 64-65 0.4086707037228834 1103 66-67 0.4357168485448284 1103 68-69 0.4288094838795544 1103 70-71 0.46628177902488943 1103 72-73 0.48754764089385105 1103 74-75 0.4881215300569721 1103 76-77 0.45726948294668546 1103 78-79 0.38928720249501225 1103 80-81 0.41246912540010783 1103 82-83 0.39630367510552134 1103 84-85 0.4383427584882966 1103 86-87 0.47218759728670534 1103 88-89 0.5425038882726412 1103 90-91 0.593456463749888 1103 92-93 0.6824980632609687 1103 94-95 0.5677608047433687 1103 96-97 0.5836421410748578 1103 98-99 0.6567921385605331 1103 100 0.6807573532687456 1104 1 0.18332702352297403 1104 2 0.1937963079517182 1104 3 0.30442963797165845 1104 4 0.24715271079496404 1104 5 0.17959015215053853 1104 6 0.19974459963714963 1104 7 0.21761034971192572 1104 8 0.21612356522123832 1104 9 0.21739219041194957 1104 10-11 0.24187465540690312 1104 12-13 0.19491650894957502 1104 14-15 0.29764142992404885 1104 16-17 0.23151326268871486 1104 18-19 0.2914074409778209 1104 20-21 0.3271933455178768 1104 22-23 0.3036897030285317 1104 24-25 0.2875650325400727 1104 26-27 0.3357672106479299 1104 28-29 0.3584940541471582 1104 30-31 0.36573433891864937 1104 32-33 0.3366993206493163 1104 34-35 0.26955820755163984 1104 36-37 0.3020977612315434 1104 38-39 0.3273230991195888 1104 40-41 0.29017540721415713 1104 42-43 0.2772606449042385 1104 44-45 0.22565991708030708 1104 46-47 0.2960500276009128 1104 48-49 0.23187121637154462 1104 50-51 0.18669347129877423 1104 52-53 0.1448428918104483 1104 54-55 0.12068780613127927 1104 56-57 0.1723800028923037 1104 58-59 0.23852866773535908 1104 60-61 0.24841205109504472 1104 62-63 0.2056358102110991 1104 64-65 0.15381208876547703 1104 66-67 0.18503019785842412 1104 68-69 0.09158349555070089 1104 70-71 0.140538234097086 1104 72-73 0.13324918821287213 1104 74-75 0.16038076402600865 1104 76-77 0.12956018026912375 1104 78-79 0.07626766685591235 1104 80-81 0.10793889904826415 1104 82-83 0.17943426635589077 1104 84-85 0.2190031279645126 1104 86-87 0.16428755507138249 1104 88-89 0.167367127722315 1104 90-91 0.13875346587001403 1104 92-93 0.1820140814499389 1104 94-95 0.2088912663207907 1104 96-97 0.23798626574956216 1104 98-99 0.2520954858698339 1104 100 0.2379101670999404 1105 1 0.21361023491503772 1105 2 0.19879551196360623 1105 3 0.24433935833202014 1105 4 0.1526706526025734 1105 5 0.1548971059153601 1105 6 0.12189040236964388 1105 7 0.15547906051109806 1105 8 0.15179870131618145 1105 9 0.16306874270429006 1105 10-11 0.18584522894510513 1105 12-13 0.16120325476443043 1105 14-15 0.2265733280444593 1105 16-17 0.2453081851634593 1105 18-19 0.19212884088035054 1105 20-21 0.20069916438578161 1105 22-23 0.20059009723518528 1105 24-25 0.22803576822064997 1105 26-27 0.23912995008655002 1105 28-29 0.24020611629819655 1105 30-31 0.24082249335610584 1105 32-33 0.25603116723210206 1105 34-35 0.269072976460933 1105 36-37 0.3053784826350707 1105 38-39 0.2516456416569284 1105 40-41 0.29127275633364036 1105 42-43 0.30077858254389156 1105 44-45 0.2727104436148977 1105 46-47 0.3006337955866414 1105 48-49 0.2641562495208092 1105 50-51 0.2827605742291439 1105 52-53 0.2568700501818242 1105 54-55 0.16281079929665054 1105 56-57 0.2540065767356765 1105 58-59 0.2834416150867938 1105 60-61 0.2439945028235968 1105 62-63 0.26548433054757226 1105 64-65 0.18574622571004085 1105 66-67 0.24279650027936128 1105 68-69 0.22291750185717518 1105 70-71 0.2344261598771098 1105 72-73 0.3113322784987602 1105 74-75 0.27357349000848785 1105 76-77 0.2641538870068132 1105 78-79 0.2652643113832056 1105 80-81 0.2849731968485685 1105 82-83 0.22944485385853852 1105 84-85 0.26355988762512084 1105 86-87 0.20665654797743827 1105 88-89 0.27677822608896463 1105 90-91 0.30415501129203193 1105 92-93 0.3028246447059857 1105 94-95 0.33499614266990463 1105 96-97 0.3958272651083341 1105 98-99 0.4172599110998192 1105 100 0.4864068006184077 1106 1 0.16988096297492206 1106 2 0.1978706683496405 1106 3 0.34650889287959785 1106 4 0.23840955643659356 1106 5 0.17090646778472518 1106 6 0.14091968513747588 1106 7 0.20280420386377074 1106 8 0.19937133534068607 1106 9 0.2348584153539548 1106 10-11 0.16143436082001728 1106 12-13 0.18276903047104298 1106 14-15 0.28468007023652575 1106 16-17 0.2726283099552873 1106 18-19 0.2852257225933599 1106 20-21 0.28449337589213997 1106 22-23 0.22991564123248054 1106 24-25 0.25848915165300923 1106 26-27 0.32162302139803955 1106 28-29 0.23913470702787976 1106 30-31 0.2935946275869483 1106 32-33 0.22671631727617836 1106 34-35 0.28535809182577054 1106 36-37 0.2877386613859656 1106 38-39 0.29200953696913956 1106 40-41 0.3734611429484431 1106 42-43 0.3434303343329006 1106 44-45 0.28881786602437387 1106 46-47 0.2966812015081999 1106 48-49 0.3495543762685003 1106 50-51 0.35987941233682363 1106 52-53 0.4049531146204899 1106 54-55 0.3858644407991605 1106 56-57 0.34427459805730365 1106 58-59 0.3554737178351459 1106 60-61 0.33474690693003595 1106 62-63 0.33993175892661753 1106 64-65 0.26142900683299786 1106 66-67 0.28512322959656444 1106 68-69 0.3515818103604076 1106 70-71 0.40502390522944864 1106 72-73 0.4340304577045373 1106 74-75 0.38850225718984177 1106 76-77 0.3412266070610599 1106 78-79 0.39876920132910243 1106 80-81 0.3711486467287557 1106 82-83 0.3795699073048766 1106 84-85 0.3714134961288664 1106 86-87 0.47156571259903046 1106 88-89 0.42593336817553507 1106 90-91 0.4633069814495485 1106 92-93 0.5236018876331663 1106 94-95 0.4782306051348719 1106 96-97 0.5151727668603421 1106 98-99 0.46352376632847125 1106 100 0.48748594397222433 1107 1 0.1333551408462057 1107 2 0.08286397204342677 1107 3 0.23946063397322348 1107 4 0.17859547125369346 1107 5 0.14893960960599628 1107 6 0.13824681660705096 1107 7 0.14340624965653603 1107 8 0.14190716824128202 1107 9 0.12887318408802173 1107 10-11 0.18614772394411006 1107 12-13 0.1290219411600475 1107 14-15 0.21107389972435442 1107 16-17 0.1594373850265498 1107 18-19 0.1128378710389768 1107 20-21 0.13657015570269238 1107 22-23 0.11133494591568649 1107 24-25 0.11220340077694146 1107 26-27 0.1897211475664733 1107 28-29 0.2133627466000192 1107 30-31 0.19482543587478318 1107 32-33 0.21703673205156093 1107 34-35 0.22715462389661667 1107 36-37 0.18101996045722046 1107 38-39 0.253697588744366 1107 40-41 0.22408348964090408 1107 42-43 0.2798812728014113 1107 44-45 0.2741288442670111 1107 46-47 0.32246579472464276 1107 48-49 0.366529974952968 1107 50-51 0.31694691831347654 1107 52-53 0.27836539165894436 1107 54-55 0.3209494769345014 1107 56-57 0.34318495464184906 1107 58-59 0.3954029921092044 1107 60-61 0.2869477851122184 1107 62-63 0.31439990586918753 1107 64-65 0.28364825074380917 1107 66-67 0.2876982340994658 1107 68-69 0.3007281528364629 1107 70-71 0.2919330805934095 1107 72-73 0.3568723554778188 1107 74-75 0.37397803551814235 1107 76-77 0.35171544180251857 1107 78-79 0.3904320503113681 1107 80-81 0.38630319138667346 1107 82-83 0.36467741073234805 1107 84-85 0.33452163536450996 1107 86-87 0.281412194091466 1107 88-89 0.280107393420451 1107 90-91 0.29350803299713135 1107 92-93 0.33553233198867716 1107 94-95 0.3451610717686471 1107 96-97 0.36933120575889333 1107 98-99 0.3863469132291897 1107 100 0.3695017238694511 1108 1 0.14535875196041204 1108 2 0.05268892402526859 1108 3 0.15610729860187078 1108 4 0.08239903624711786 1108 5 0.09708221969216879 1108 6 0.08511526701764893 1108 7 0.06591686168307831 1108 8 0.06105830032345949 1108 9 0.15733253490478916 1108 10-11 0.14188905188306222 1108 12-13 0.10311887070457715 1108 14-15 0.18008447353804513 1108 16-17 0.09304987449811364 1108 18-19 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-0.5169555179238614 2215 28-29 -0.46286313371902565 2215 30-31 -0.48331484594079654 2215 32-33 -0.40879502675016965 2215 34-35 -0.5348823383850103 2215 36-37 -0.494087871516264 2215 38-39 -0.4941242635380192 2215 40-41 -0.4839754575101196 2215 42-43 -0.456531215085171 2215 44-45 -0.49295222073008915 2215 46-47 -0.569854996666848 2215 48-49 -0.536820970484456 2215 50-51 -0.5545558223213476 2215 52-53 -0.5474152968509713 2215 54-55 -0.4464181820402189 2215 56-57 -0.4772795053912233 2215 58-59 -0.469670784073017 2215 60-61 -0.4585331615332109 2215 62-63 -0.47891245380204595 2215 64-65 -0.45591108559538185 2215 66-67 -0.46415256356578993 2215 68-69 -0.5028973203449922 2215 70-71 -0.5650655434267051 2215 72-73 -0.47398845787674304 2215 74-75 -0.4856076669190088 2215 76-77 -0.39155770527334965 2215 78-79 -0.500081089294909 2215 80-81 -0.5966673984209265 2215 82-83 -0.46908512859135953 2215 84-85 -0.4454280137207398 2215 86-87 -0.4305795125838934 2215 88-89 -0.5286613137320089 2215 90-91 -0.5218963643786054 2215 92-93 -0.4879847390147809 2215 94-95 -0.5774347247190654 2215 96-97 -0.583432853724549 2215 98-99 -0.5450336629933616 2215 100 -0.5006470474479165 2216 1 -0.6464848332709145 2216 2 -0.6711060842850181 2216 3 -0.7626533863941383 2216 4 -0.521426586672284 2216 5 -0.7287201555519047 2216 6 -0.5994000782176769 2216 7 -0.8064045617788622 2216 8 -0.5610366390071135 2216 9 -0.9171197395989665 2216 10-11 -0.671313410256964 2216 12-13 -0.6092497307151916 2216 14-15 -0.9835747792888228 2216 16-17 -0.7830277593025059 2216 18-19 -0.8530820037300728 2216 20-21 -0.8080123050907915 2216 22-23 -0.829538961261477 2216 24-25 -0.7883394256714809 2216 26-27 -0.9458922849845663 2216 28-29 -0.9709572604277099 2216 30-31 -1.0677701361261924 2216 32-33 -1.0824922001255786 2216 34-35 -1.024920287723539 2216 36-37 -1.114813676561461 2216 38-39 -1.0382035081967373 2216 40-41 -1.0007009974597239 2216 42-43 -1.0906868098981803 2216 44-45 -1.2964644443164275 2216 46-47 -1.1912038975369228 2216 48-49 -1.375079569240505 2216 50-51 -1.1596196092208615 2216 52-53 -1.0893303461394268 2216 54-55 -1.0681983345755626 2216 56-57 -1.2005347731138656 2216 58-59 -1.227660273103801 2216 60-61 -1.0750940722095947 2216 62-63 -0.9831147776877174 2216 64-65 -1.1318501161697156 2216 66-67 -1.0260632822414664 2216 68-69 -0.9867581368811287 2216 70-71 -1.1133874200341083 2216 72-73 -1.0558687706881855 2216 74-75 -0.9845925501412722 2216 76-77 -1.0196231128448474 2216 78-79 -1.2495022321794096 2216 80-81 -1.4257259111505292 2216 82-83 -1.2954137274625523 2216 84-85 -1.4032034028850333 2216 86-87 -1.148532818121133 2216 88-89 -1.561723722565425 2216 90-91 -1.3420395146474 2216 92-93 -1.4635666632127986 2216 94-95 -1.45179027546844 2216 96-97 -1.5139833657455242 2216 98-99 -1.6458897308373608 2216 100 -1.6280070933753734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 212.0 3 24.0 4 32.0 5 67.0 6 192.0 7 613.0 8 1393.0 9 2549.0 10 4107.0 11 6074.0 12 8799.0 13 11546.0 14 14284.0 15 18006.0 16 22366.0 17 30273.0 18 29783.0 19 17239.0 20 13961.0 21 14583.0 22 15993.0 23 18501.0 24 22733.0 25 27887.0 26 33266.0 27 37696.0 28 48451.0 29 62903.0 30 81905.0 31 105870.0 32 134993.0 33 176531.0 34 237768.0 35 338915.0 36 541875.0 37 965853.0 38 1341207.0 39 544553.0 40 2307.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.17459663369712 21.59750102512535 15.257148868470388 22.970753472707138 2 15.405597622033874 20.701698576178597 40.50523675311176 23.387467048675767 3 17.539283246645095 25.79967621081553 31.419809495249538 25.241231047289837 4 12.594084787801535 18.063711917531407 38.29948641513632 31.042716879530747 5 12.441102990490972 37.88274698043284 36.0708445872701 13.605305441806086 6 29.9083137634718 38.83903949295991 17.317189801653797 13.93545694191449 7 25.661245190271735 33.44572073486772 22.70943466570489 18.183599409155658 8 24.112270651682334 37.117469132500474 20.42243532697585 18.34782488884134 9 24.06252089534396 17.02547560335622 22.06009348956803 36.85191001173179 10-11 23.37707256484395 27.385392204339748 28.953875237827003 20.283659992989296 12-13 24.15062632585567 24.819024818903195 29.22707365600171 21.80327519923943 14-15 21.454090182047477 26.932342189116543 26.82441575051274 24.78915187832325 16-17 20.41798999637206 30.340569319918032 27.668406611581297 21.573034072128607 18-19 20.238759185724497 28.51931718540334 30.591680457600667 20.650243171271494 20-21 21.233428717460868 27.69750415754236 30.463269006034576 20.60579811896219 22-23 20.96993907170978 27.75225872336287 30.462199942860735 20.81560226206662 24-25 20.944288622632552 27.88911115377426 30.433899441827528 20.732700781765658 26-27 20.69637592057315 27.839364043808 30.590701675487963 20.873558360130883 28-29 20.62517032923057 27.655773363091768 30.83346588197869 20.88559042569897 30-31 20.982647692373465 28.161225568652338 30.242564346021698 20.6135623929525 32-33 20.956257687600242 27.682290534833587 30.578405392254147 20.78304638531202 34-35 20.883030918938502 27.47034962454728 30.608755258156606 21.03786419835761 36-37 21.017952885200454 27.910127981906697 30.141367092259912 20.930552040632932 38-39 20.881333245395336 27.635356383422227 30.452908037624088 21.030402333558346 40-41 20.737538867904036 27.441337983906166 30.619084206734094 21.202038941455704 42-43 20.844664578100815 27.423596349916608 30.47470718253426 21.25703188944832 44-45 20.759978655790995 27.65296877300077 30.28631613115848 21.300736440049754 46-47 20.989829502921246 27.675688501084384 29.822931898123002 21.511550097871368 48-49 20.89153222191097 27.65954429015246 29.617128149588652 21.83179533834792 50-51 20.721267071195655 27.696170455740464 29.553924624849575 22.0286378482143 52-53 20.652076015491307 27.747635976737712 29.723277295012533 21.877010712758445 54-55 20.76905768287211 27.992376936982282 29.35836653049423 21.880198849651382 56-57 20.803860609020738 27.74623788076207 29.176834522272127 22.27306698794507 58-59 20.541918040096217 27.618737125285204 29.500343798512084 22.33900103610649 60-61 20.816603088951965 28.065273052886262 28.863968410421375 22.254155447740395 62-63 20.435571165653247 28.193256818729363 28.90956051617876 22.461611499438632 64-65 20.432355764481805 27.99367817984261 28.97207102929667 22.60189502637891 66-67 20.45703819860177 28.112309996306152 28.708857312309267 22.721794492782813 68-69 20.858873764862654 28.253695546742954 28.4402825764673 22.447148111927092 70-71 20.87267414883421 28.008394643475864 28.388504019014082 22.730427188675844 72-73 20.687870687232422 28.09041024999904 28.439480075919015 22.782238986849528 74-75 20.904297201888937 27.98473757602946 28.302573036708516 22.808392185373087 76-77 20.992850348475987 28.05262847028499 28.081593418645017 22.872927762594003 78-79 21.052562791974704 28.060411598848667 28.1528404940483 22.73418511512833 80-81 20.905429627519307 27.99549236482059 28.142173885380515 22.956904122279585 82-83 20.84776817396881 27.878796474952395 28.26950596752648 23.00392938355231 84-85 21.00271864836349 27.685230082106543 28.111304304833894 23.20074696469607 86-87 21.045986572095206 27.84905308652357 28.044960280593973 23.060000060787257 88-89 20.888993308594777 27.61786399630492 28.163737258271503 23.329405436828797 90-91 20.761557537265293 27.44757368436889 28.229134510262373 23.561734268103436 92-93 20.861759330910324 27.515078974781687 28.14532916003317 23.477832534274818 94-95 20.757602912911235 27.248402600822285 28.35315533132197 23.640839154944505 96-97 20.82704723119891 27.205311196122434 28.32815231199721 23.639489260681444 98-99 20.773140383585492 27.291891202959327 28.2652110737399 23.669757339715282 100 20.838673322733392 27.27478482839866 28.18426311793513 23.702278730932818 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 351.0 1 280.0 2 354.0 3 498.5 4 328.5 5 229.5 6 587.5 7 1701.5 8 2985.5 9 3472.5 10 2838.5 11 2041.0 12 2025.5 13 2290.5 14 2620.0 15 3119.0 16 3668.5 17 4221.0 18 4869.5 19 5846.5 20 7316.5 21 9160.5 22 11797.0 23 15889.0 24 21288.5 25 28002.0 26 36977.5 27 48350.0 28 61343.0 29 74961.0 30 88986.5 31 101635.5 32 112291.0 33 123116.0 34 134198.5 35 144446.0 36 152727.0 37 160963.5 38 169064.5 39 173504.5 40 177926.5 41 182658.0 42 184348.5 43 184384.0 44 182964.5 45 180935.0 46 178320.5 47 172297.0 48 165139.0 49 160297.5 50 155284.0 51 147500.0 52 136592.5 53 126942.5 54 119443.5 55 112238.5 56 102605.5 57 90913.5 58 80749.5 59 71473.0 60 61464.5 61 50823.0 62 41692.0 63 34071.0 64 27371.0 65 21658.5 66 17028.0 67 12989.0 68 9584.5 69 7175.5 70 5392.0 71 3977.5 72 2856.5 73 1948.5 74 1388.5 75 999.0 76 693.0 77 429.5 78 249.5 79 162.0 80 83.0 81 33.0 82 19.0 83 10.0 84 9.0 85 6.5 86 3.5 87 2.5 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035803222087366346 2 0.0 3 0.0 4 2.026215171893964E-5 5 0.0 6 0.0 7 0.0 8 4.457673378166721E-4 9 0.0 10-11 0.0 12-13 0.04407017998869372 14-15 8.104860687575856E-5 16-17 1.013107585946982E-5 18-19 5.06553792973491E-5 20-21 5.06553792973491E-5 22-23 0.0 24-25 1.5196613789204732E-4 26-27 3.0393227578409465E-5 28-29 3.0393227578409465E-4 30-31 2.026215171893964E-5 32-33 1.7222828961098696E-4 34-35 3.0393227578409465E-5 36-37 6.078645515681893E-5 38-39 2.9380119992462476E-4 40-41 8.104860687575856E-5 42-43 5.166848688329609E-4 44-45 2.7353904820568516E-4 46-47 1.1144183445416803E-4 48-49 8.611414480549347E-4 50-51 4.660294895356117E-4 52-53 5.369470205519005E-4 54-55 4.761605653950816E-4 56-57 5.369470205519005E-4 58-59 1.3170398617310766E-4 60-61 2.3301474476780586E-4 62-63 8.712725239144046E-4 64-65 4.558984136761419E-4 66-67 0.0015601856823583525 68-69 0.0016108410616557014 70-71 4.052430343787928E-4 72-73 0.0010333697376659217 74-75 0.0010536318893848613 76-77 6.990442343034176E-4 78-79 0.003343255033625041 80-81 0.002593555420024274 82-83 0.0011650737238390295 84-85 0.002775914785494731 86-87 0.0013170398617310766 88-89 0.0019958219443155546 90-91 0.0024618514338511665 92-93 0.0025732932683053345 94-95 0.0036167940818307262 96-97 0.0030494538337004157 98-99 0.0028164390889326104 100 0.0012765155582931975 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4935310.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.71763085296968 #Duplication Level Percentage of deduplicated Percentage of total 1 78.51054353217853 51.59526917913715 2 13.555093455423032 17.816172557619918 3 3.846118339110856 7.5827335567957235 4 1.5886705884214405 4.176146691074014 5 0.7658424005377851 2.5164674085047154 6 0.44848584523794893 1.768405633207777 7 0.2816647382062275 1.2957237502814645 8 0.18815441802245453 0.989205006956401 9 0.13520793369371392 0.7996990567388663 >10 0.6349058114661938 7.229118192234023 >50 0.032216572499101315 1.425288467395828 >100 0.012233846335631614 1.3393495512925058 >500 5.236724372018788E-4 0.25100318982052017 >1k 1.8482532538397048E-4 0.24482856770904077 >5k 6.160844179419974E-5 0.2544848623001345 >10k+ 9.241266269129962E-5 0.7161043289320627 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 13368 0.27086444417878514 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11263 0.22821261481041719 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10743 0.21767629591656856 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 6666 0.13506750335845163 RNA PCR Primer, Index 46 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5905 0.11964800590033857 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.052430343787928E-5 0.0 5 0.0 0.0 0.0 4.052430343787928E-5 0.0 6 0.0 0.0 0.0 4.052430343787928E-5 0.0 7 0.0 0.0 0.0 8.104860687575856E-5 0.0 8 0.0 0.0 0.0 8.104860687575856E-5 0.0 9 0.0 0.0 0.0 1.2157291031363785E-4 0.0 10-11 0.0 0.0 0.0 1.8235936547045676E-4 0.0 12-13 0.0 0.0 0.0 2.330147447678059E-4 0.0 14-15 0.0 0.0 0.0 2.634079723462153E-4 0.0 16-17 0.0 0.0 0.0 2.938011999246248E-4 0.0 18-19 0.0 0.0 0.0 3.7484980680038335E-4 0.0 20-21 0.0 0.0 0.0 5.6734024813031E-4 0.0 22-23 0.0 0.0 0.0 0.001408219544466305 0.0 24-25 0.0 0.0 0.0 0.003343255033625041 0.0 26-27 0.0 0.0 0.0 0.00788197701866752 0.0 28-29 0.0 0.0 0.0 0.027252594061973816 0.0 30-31 0.0 0.0 0.0 0.08740079143964614 0.0 32-33 0.0 0.0 0.0 0.2186387481232182 0.0 34-35 0.0 0.0 0.0 0.41939614735447217 0.0 36-37 0.0 0.0 0.0 0.7249595263519414 0.0 38-39 0.0 2.026215171893964E-5 0.0 1.1137800867625338 0.0 40-41 0.0 2.026215171893964E-5 0.0 1.4990243773947332 0.0 42-43 0.0 2.026215171893964E-5 0.0 1.8690011367067114 0.0 44-45 0.0 2.026215171893964E-5 0.0 2.3086189114766853 0.0 46-47 0.0 2.026215171893964E-5 0.0 2.818323469042472 0.0 48-49 0.0 2.026215171893964E-5 0.0 3.3213212543892885 0.0 50-51 0.0 3.039322757840946E-5 0.0 3.7994573795769666 0.0 52-53 0.0 4.052430343787928E-5 0.0 4.244708032524805 0.0 54-55 0.0 5.06553792973491E-5 0.0 4.724110542194918 0.0 56-57 0.0 6.078645515681892E-5 0.0 5.261857917739716 0.0 58-59 0.0 6.078645515681892E-5 0.0 5.825723206850228 0.0 60-61 0.0 7.091753101628875E-5 0.0 6.370531942269078 0.0 62-63 0.0 8.104860687575856E-5 0.0 6.882992152468639 0.0 64-65 0.0 8.104860687575856E-5 0.0 7.40368892734195 0.0 66-67 0.0 8.104860687575856E-5 0.0 7.976692446877704 0.0 68-69 0.0 8.104860687575856E-5 0.0 8.575145228972445 0.0 70-71 0.0 8.104860687575856E-5 0.0 9.179311937851928 0.0 72-73 0.0 8.104860687575856E-5 0.0 9.770237330583084 0.0 74-75 0.0 8.104860687575856E-5 0.0 10.345246803139013 0.0 76-77 0.0 8.104860687575856E-5 0.0 10.945594096419477 0.0 78-79 0.0 8.104860687575856E-5 0.0 11.582413262794029 0.0 80-81 0.0 8.104860687575856E-5 0.0 12.234814023840448 0.0 82-83 0.0 8.104860687575856E-5 0.0 12.880041983178362 0.0 84-85 0.0 8.104860687575856E-5 0.0 13.495494710565293 0.0 86-87 0.0 8.104860687575856E-5 0.0 14.123064204680151 0.0 88 0.0 8.104860687575856E-5 0.0 14.610652623644715 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8250 0.0 58.294136 1 GTATCAA 15040 0.0 40.572342 1 GTGGTAT 3325 0.0 35.488304 1 ATCAACG 18930 0.0 31.901777 3 TCAACGC 18985 0.0 31.858868 4 TGGTATC 3545 0.0 31.021442 2 CAACGCA 19650 0.0 30.804607 5 TATCAAC 19770 0.0 30.712717 2 AACGCAG 20470 0.0 29.619984 6 ACGCAGA 23055 0.0 25.972456 7 CGCAGAG 23175 0.0 25.71619 8 GCAGAGT 25345 0.0 23.143225 9 GAGTACT 17600 0.0 21.59849 12-13 GTACATG 12580 0.0 20.628214 1 CAGAGTA 24910 0.0 20.507174 10-11 AGAGTAC 23705 0.0 20.497932 10-11 AGTACTT 18605 0.0 19.522024 12-13 GTACTTT 19650 0.0 19.324629 14-15 TACATGG 12675 0.0 19.095087 2 ACATGGG 12920 0.0 17.859997 3 >>END_MODULE