FastQCFastQC Report
Wed 25 May 2016
SRR2049346_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049346_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5246857
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55300.046.8426441
GTATCAA91250.035.806911
TCAACGC99850.031.4978264
ATCAACG100800.031.2942473
CAACGCA107000.029.4375615
AACGCAG109850.028.6691726
TATCAAC114500.027.8372762
ACGCAGA128850.024.0764067
CGCAGAG130600.023.5378468
GTACATG148250.023.0861571
TACATGG146500.021.9172272
ACATGGG152700.020.5039583
CGTATCA13900.019.94855194
GTGGTAT32150.019.7409361
GCCGTAT21150.019.3322894
GCAGAGT150750.019.2693659
CGCCGTA25100.018.9113294
CATGGGG106600.017.8166774
GAGTACA115100.017.6859131
AGAGTAC129200.017.55379710-11
Produced by FastQC (version 0.11.5)