##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049346_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5246857
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.737582709038954	31.0	31.0	34.0	28.0	34.0
2	31.02045262525737	31.0	31.0	34.0	28.0	34.0
3	31.091802006420224	31.0	31.0	34.0	28.0	34.0
4	34.703953433455496	37.0	35.0	37.0	32.0	37.0
5	34.658149059522685	37.0	35.0	37.0	32.0	37.0
6	34.73649577261206	37.0	35.0	37.0	32.0	37.0
7	34.67765730989047	37.0	35.0	37.0	32.0	37.0
8	34.65367285595929	37.0	35.0	37.0	32.0	37.0
9	36.22858808616282	39.0	37.0	39.0	32.0	39.0
10-11	36.187236854368244	39.0	37.0	39.0	32.0	39.0
12-13	36.11893939552765	39.0	37.0	39.0	32.0	39.0
14-15	37.33013668945047	40.0	38.0	41.0	32.0	41.0
16-17	37.30169775924901	40.0	38.0	41.0	32.0	41.0
18-19	37.34052042965913	40.0	38.0	41.0	32.0	41.0
20-21	37.41258643031438	40.0	38.0	41.0	32.0	41.0
22-23	37.36631863609014	40.0	38.0	41.0	32.0	41.0
24-25	37.374210598840406	40.0	38.0	41.0	32.0	41.0
26-27	37.356631503393366	40.0	38.0	41.0	32.0	41.0
28-29	37.29177067337646	40.0	38.0	41.0	32.0	41.0
30-31	37.222391519341954	40.0	37.0	41.0	31.0	41.0
32-33	37.15648015564365	40.0	37.0	41.0	31.0	41.0
34-35	37.04671510963611	40.0	37.0	41.0	31.0	41.0
36-37	36.95898878128373	40.0	37.0	41.0	30.5	41.0
38-39	36.85187694271066	40.0	37.0	41.0	30.0	41.0
40-41	36.74707639259084	40.0	37.0	41.0	30.0	41.0
42-43	36.617855508545404	40.0	37.0	41.0	30.0	41.0
44-45	36.50581100266312	40.0	36.5	41.0	30.0	41.0
46-47	36.39189385188123	40.0	36.0	41.0	30.0	41.0
48-49	36.267258189045364	40.0	36.0	41.0	30.0	41.0
50-51	36.13213462840706	40.0	36.0	41.0	29.0	41.0
52-53	35.99044809111436	39.0	35.0	41.0	29.0	41.0
54-55	35.85532919993817	39.0	35.0	41.0	29.0	41.0
56-57	35.72400315083868	39.0	35.0	41.0	28.0	41.0
58-59	35.52097246027479	39.0	35.0	41.0	28.0	41.0
60-61	35.30050618875262	39.0	35.0	40.5	28.0	41.0
62-63	35.085341376751835	38.0	34.5	40.0	27.5	41.0
64-65	34.836320200836425	38.0	34.0	40.0	27.0	41.0
66-67	34.56530338067151	37.5	34.0	40.0	26.5	41.0
68-69	34.26010619309808	37.0	34.0	40.0	26.0	41.0
70-71	33.95269720520304	36.5	34.0	39.0	26.0	41.0
72-73	33.58605132558406	36.0	33.5	39.0	26.0	41.0
74-75	33.21018707008786	35.5	33.0	39.0	25.0	40.5
76-77	32.84859478731744	35.0	33.0	38.0	25.0	40.0
78-79	32.49280159531697	35.0	33.0	37.0	24.0	39.0
80-81	32.10139984756589	35.0	33.0	37.0	22.5	39.0
82-83	31.74899782860482	35.0	32.0	36.0	21.5	39.0
84-85	31.421498527594707	35.0	32.0	36.0	20.0	37.0
86-87	31.060577694417823	35.0	32.0	35.5	18.5	37.0
88-89	30.75177234675921	35.0	32.0	35.0	16.5	36.5
90-91	30.47817197991102	35.0	31.5	35.0	9.0	36.0
92-93	30.166757451175055	35.0	31.0	35.0	2.0	36.0
94-95	29.882609436468346	35.0	31.0	35.0	2.0	36.0
96-97	29.549684411067425	34.0	31.0	35.0	2.0	35.5
98-99	29.263611434426362	34.0	31.0	35.0	2.0	35.0
100	29.089488049702897	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22383387066059157
1101	2	0.29224616638542855
1101	3	0.22117809989886084
1101	4	0.2751653861576031
1101	5	0.2343122947272036
1101	6	0.3629897395722992
1101	7	0.3621422272811401
1101	8	0.3902445548767588
1101	9	0.5470556854988615
1101	10-11	0.5127450272008858
1101	12-13	0.5402800222773649
1101	14-15	0.6263609715141385
1101	16-17	0.5868143809160955
1101	18-19	0.5438981870882031
1101	20-21	0.4771844516294337
1101	22-23	0.5118447092464962
1101	24-25	0.5515654638898226
1101	26-27	0.5046206540318181
1101	28-29	0.5793613845365329
1101	30-31	0.605464251957649
1101	32-33	0.521881515070973
1101	34-35	0.5796385878542694
1101	36-37	0.5621446319073158
1101	38-39	0.641588055219863
1101	40-41	0.6494616137332798
1101	42-43	0.4899393512421355
1101	44-45	0.5670284286633844
1101	46-47	0.5618280724815321
1101	48-49	0.5250572512737222
1101	50-51	0.5125532025014081
1101	52-53	0.5593774138434
1101	54-55	0.48728092420456903
1101	56-57	0.5345100820511348
1101	58-59	0.5076136662718156
1101	60-61	0.5306750697563132
1101	62-63	0.52300516906962
1101	64-65	0.5240318429886486
1101	66-67	0.5285995131414296
1101	68-69	0.5357398521443244
1101	70-71	0.5356558108578398
1101	72-73	0.6079293291927144
1101	74-75	0.5668339569436753
1101	76-77	0.6104943590805973
1101	78-79	0.5885002890386062
1101	80-81	0.5210857157124238
1101	82-83	0.5595549394202308
1101	84-85	0.5309302265683691
1101	86-87	0.5578271129087398
1101	88-89	0.6242186124821956
1101	90-91	0.6185392141204673
1101	92-93	0.5322284418657866
1101	94-95	0.5412001061573228
1101	96-97	0.5963886926179747
1101	98-99	0.5963108655242593
1101	100	0.6421159859249101
1102	1	0.15513747821545465
1102	2	0.22888292452790893
1102	3	0.26371412139593176
1102	4	0.28428888236626904
1102	5	0.1913295564756936
1102	6	0.32505545467429897
1102	7	0.43163703833236866
1102	8	0.3989594406735577
1102	9	0.4697599617486077
1102	10-11	0.4260725178190867
1102	12-13	0.4441684038967537
1102	14-15	0.5461164172077773
1102	16-17	0.5597406159940093
1102	18-19	0.5171708346875477
1102	20-21	0.4175950310620422
1102	22-23	0.5131114941831001
1102	24-25	0.5466032131060743
1102	26-27	0.47090537250046083
1102	28-29	0.5521494696445544
1102	30-31	0.521787279462167
1102	32-33	0.5455976845920745
1102	34-35	0.5519851972777658
1102	36-37	0.5825229155389593
1102	38-39	0.5420363356084152
1102	40-41	0.5739400838427713
1102	42-43	0.5099687996846782
1102	44-45	0.42990266808086375
1102	46-47	0.40763953102398887
1102	48-49	0.4008075640005444
1102	50-51	0.46587661029718674
1102	52-53	0.48973925036062127
1102	54-55	0.4375156141031056
1102	56-57	0.4228627935085143
1102	58-59	0.5455336854215034
1102	60-61	0.4957397192706168
1102	62-63	0.44411676814611667
1102	64-65	0.40581888374352104
1102	66-67	0.4677375163999642
1102	68-69	0.5441830420351437
1102	70-71	0.5024158161857741
1102	72-73	0.4724364755797552
1102	74-75	0.5130187669138664
1102	76-77	0.49511831472744205
1102	78-79	0.4436998261256946
1102	80-81	0.38850590280800645
1102	82-83	0.4355453858088154
1102	84-85	0.40093678997083515
1102	86-87	0.4188654528312128
1102	88-89	0.410378211265261
1102	90-91	0.38813032379878365
1102	92-93	0.3248269411631206
1102	94-95	0.30960471675451373
1102	96-97	0.31127404542015924
1102	98-99	0.3791943612696258
1102	100	0.3314138562938531
1103	1	0.1711818654761892
1103	2	0.288212446052281
1103	3	0.3005990211800693
1103	4	0.30478949063849825
1103	5	0.27033536703428496
1103	6	0.34831642189313783
1103	7	0.3545602585842573
1103	8	0.3972416416678186
1103	9	0.44866805734451987
1103	10-11	0.4491251569195427
1103	12-13	0.41894183216272296
1103	14-15	0.50830074485647
1103	16-17	0.4914231930111086
1103	18-19	0.45100746455446483
1103	20-21	0.43785256710666687
1103	22-23	0.47855817262531986
1103	24-25	0.422470357166695
1103	26-27	0.42070600575895156
1103	28-29	0.41334570650344205
1103	30-31	0.43176560194157076
1103	32-33	0.44976167096204733
1103	34-35	0.45917426048391974
1103	36-37	0.42949594994126983
1103	38-39	0.45188904406965236
1103	40-41	0.4532755856867112
1103	42-43	0.42280292045709444
1103	44-45	0.35702017680898734
1103	46-47	0.3609754342866296
1103	48-49	0.4405811310791137
1103	50-51	0.38913575702301273
1103	52-53	0.46056212685792275
1103	54-55	0.4474989702456966
1103	56-57	0.387154414643895
1103	58-59	0.5316175455220815
1103	60-61	0.46381455245656866
1103	62-63	0.5048337511158536
1103	64-65	0.43664151447426036
1103	66-67	0.5487889603467622
1103	68-69	0.6156466319472429
1103	70-71	0.5124773716059039
1103	72-73	0.5386591391703917
1103	74-75	0.5327786620898891
1103	76-77	0.5705934884366073
1103	78-79	0.4569160683823412
1103	80-81	0.48146287044919234
1103	82-83	0.47096553511655515
1103	84-85	0.5200377485969447
1103	86-87	0.49446224457636845
1103	88-89	0.4379214877976949
1103	90-91	0.4947874480478873
1103	92-93	0.3786534308839329
1103	94-95	0.4250453466086874
1103	96-97	0.3706692867424408
1103	98-99	0.38934910772729125
1103	100	0.34041022304491264
1104	1	0.10555608970527075
1104	2	0.16304439191540965
1104	3	0.18633120367477218
1104	4	0.23956624499898993
1104	5	0.2255920350758558
1104	6	0.22536378847352978
1104	7	0.25317036089516165
1104	8	0.28141695464260863
1104	9	0.34045444575105677
1104	10-11	0.3524622319845605
1104	12-13	0.28623658492455206
1104	14-15	0.3894724757895247
1104	16-17	0.4543422696971433
1104	18-19	0.3576781798570252
1104	20-21	0.35589701611329616
1104	22-23	0.3811563641860758
1104	24-25	0.4149253466939129
1104	26-27	0.4024517209578917
1104	28-29	0.39831308174846924
1104	30-31	0.43187395294626185
1104	32-33	0.4254287133384054
1104	34-35	0.46122434680438573
1104	36-37	0.4269071701861762
1104	38-39	0.4754879599713746
1104	40-41	0.45672228111178015
1104	42-43	0.5127225239944124
1104	44-45	0.44915394546814724
1104	46-47	0.373308898689217
1104	48-49	0.4168518740529379
1104	50-51	0.44566693697464643
1104	52-53	0.4284370042632233
1104	54-55	0.43740093868735386
1104	56-57	0.41957180717228226
1104	58-59	0.5022246415591525
1104	60-61	0.40219037648621025
1104	62-63	0.423914375942104
1104	64-65	0.4632007889753993
1104	66-67	0.5073791568262891
1104	68-69	0.4699911735566218
1104	70-71	0.42359596488083184
1104	72-73	0.4060516477755698
1104	74-75	0.4494770354075399
1104	76-77	0.48798500202541106
1104	78-79	0.43668769518353656
1104	80-81	0.48233764205767926
1104	82-83	0.37839622104643666
1104	84-85	0.4014505710504075
1104	86-87	0.43372091558503456
1104	88-89	0.41135753491362337
1104	90-91	0.43282101052624
1104	92-93	0.4513452590750653
1104	94-95	0.47659088394994953
1104	96-97	0.43995860614728954
1104	98-99	0.44098163375118204
1104	100	0.3669792413105064
1105	1	0.14191394279480463
1105	2	0.21228277865972345
1105	3	0.19763340910902727
1105	4	0.24923255863412663
1105	5	0.1482039951320644
1105	6	0.27414330238271845
1105	7	0.2238269872784997
1105	8	0.3441968011086516
1105	9	0.3813408136274745
1105	10-11	0.3462870782342691
1105	12-13	0.2706514872991548
1105	14-15	0.36341417659452446
1105	16-17	0.3362528946758303
1105	18-19	0.3162706072283967
1105	20-21	0.351752209443255
1105	22-23	0.3425063143475384
1105	24-25	0.3032198935876522
1105	26-27	0.30992662726984577
1105	28-29	0.3711852679482419
1105	30-31	0.3544860356869961
1105	32-33	0.35469717578479276
1105	34-35	0.43279266642144876
1105	36-37	0.38750286407389467
1105	38-39	0.3618913331087512
1105	40-41	0.4186522755230868
1105	42-43	0.43065927128907333
1105	44-45	0.38797841998374594
1105	46-47	0.3398423354156179
1105	48-49	0.3601700291296055
1105	50-51	0.40308853686099866
1105	52-53	0.33143300940975706
1105	54-55	0.3802123576066663
1105	56-57	0.36050068114672484
1105	58-59	0.40495977851311693
1105	60-61	0.33114608336540385
1105	62-63	0.3535223228239559
1105	64-65	0.3532797168369797
1105	66-67	0.37365058848352106
1105	68-69	0.3538668283750397
1105	70-71	0.3701450986831034
1105	72-73	0.31172327970301694
1105	74-75	0.41860080045489667
1105	76-77	0.45308044526864677
1105	78-79	0.36366485238845314
1105	80-81	0.3688054736438886
1105	82-83	0.2950048511175112
1105	84-85	0.3210319289243522
1105	86-87	0.33333800082812104
1105	88-89	0.3999775065964002
1105	90-91	0.349880915170921
1105	92-93	0.36112976698048627
1105	94-95	0.36335948073589464
1105	96-97	0.37090257931503956
1105	98-99	0.3998222437353647
1105	100	0.4136810028394393
1106	1	0.14706709301074739
1106	2	0.14244087586128984
1106	3	0.14457678863504597
1106	4	0.20149823852308657
1106	5	0.20165972646888264
1106	6	0.24336189685062237
1106	7	0.23103922193487847
1106	8	0.22472591630301508
1106	9	0.32867122412174155
1106	10-11	0.32602102329936855
1106	12-13	0.374327206323656
1106	14-15	0.4022043229739083
1106	16-17	0.3828612468635768
1106	18-19	0.3536455322923402
1106	20-21	0.3275994009903016
1106	22-23	0.374230876623308
1106	24-25	0.40665942221246354
1106	26-27	0.3642129146051616
1106	28-29	0.40509078878420013
1106	30-31	0.33524068441069943
1106	32-33	0.3513953326600898
1106	34-35	0.37296052952209635
1106	36-37	0.319376567194837
1106	38-39	0.3295867461561528
1106	40-41	0.3755917124814232
1106	42-43	0.4027966623812773
1106	44-45	0.3634292597538291
1106	46-47	0.32177290021314775
1106	48-49	0.3521911717911905
1106	50-51	0.3390945837653021
1106	52-53	0.3497072098934737
1106	54-55	0.3986128277738743
1106	56-57	0.3227993933809472
1106	58-59	0.4075298788608279
1106	60-61	0.4757762105118246
1106	62-63	0.3909147510859441
1106	64-65	0.4235293477005442
1106	66-67	0.4377187048587672
1106	68-69	0.4725737468105322
1106	70-71	0.46193537938017215
1106	72-73	0.45858720704276834
1106	74-75	0.45999767424458327
1106	76-77	0.532950668620991
1106	78-79	0.4120247734801836
1106	80-81	0.42800686295825585
1106	82-83	0.40836209178497995
1106	84-85	0.4902089948017583
1106	86-87	0.565181543741037
1106	88-89	0.4886338056882664
1106	90-91	0.5405161982503621
1106	92-93	0.4786829922372391
1106	94-95	0.448085845429965
1106	96-97	0.40136501396561286
1106	98-99	0.41193364249940245
1106	100	0.30384310664478775
1107	1	-0.0036374164152164212
1107	2	0.05928282949930619
1107	3	0.004752605859394521
1107	4	0.010103105687733205
1107	5	0.011263757652102413
1107	6	0.13748587485769548
1107	7	0.11965550103258238
1107	8	0.16218006188063327
1107	9	0.14104354789055407
1107	10-11	0.14869573152526527
1107	12-13	0.12690607616261218
1107	14-15	0.13944031838651227
1107	16-17	0.18717902239826145
1107	18-19	0.17235356323875806
1107	20-21	0.10522646431823546
1107	22-23	0.12288692547084423
1107	24-25	0.093043819167967
1107	26-27	0.14281593531161008
1107	28-29	0.22968432583954979
1107	30-31	0.1849847798704971
1107	32-33	0.22112366155187857
1107	34-35	0.2066509532536429
1107	36-37	0.18780396051421633
1107	38-39	0.24411411058152765
1107	40-41	0.2910225784284748
1107	42-43	0.23432819447126008
1107	44-45	0.20052054491275584
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2216	98-99	-2.8957638388443883
2216	100	-2.5074541393462084
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91522.0
3	13302.0
4	8428.0
5	6995.0
6	6638.0
7	7404.0
8	8775.0
9	9900.0
10	11262.0
11	12405.0
12	13751.0
13	15422.0
14	16760.0
15	17185.0
16	17306.0
17	18130.0
18	17290.0
19	16454.0
20	16414.0
21	17302.0
22	19169.0
23	22682.0
24	27583.0
25	34147.0
26	41700.0
27	49089.0
28	59461.0
29	74554.0
30	89894.0
31	108146.0
32	139448.0
33	185057.0
34	254737.0
35	367485.0
36	594073.0
37	1024154.0
38	1330859.0
39	480802.0
40	1172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.522443925450986	20.761078998073216	14.766799871598678	22.949677204877116
2	15.324819385900623	20.410289341003537	40.74909950343992	23.515791769655916
3	18.29091625988451	25.46475361794928	30.537776115185604	25.706554006980596
4	12.664238545201611	17.22098943343875	38.98807129082583	31.126700730533813
5	13.07668473312645	37.803862122880524	35.65949251838823	13.459960625604797
6	30.499209855150276	38.02865724187585	17.931597556957545	13.540535346016327
7	26.50710791856334	32.853135557058025	22.894689777173742	17.7450667472049
8	24.292596280236474	36.78018861556965	20.822988051652494	18.104227052541383
9	24.500916922511372	17.38909508576057	21.98747387371447	36.12251411801359
10-11	23.50784373991838	27.358260504144	29.15410475963126	19.97979099630636
12-13	23.89733491043389	24.987799461374465	29.097638725057774	22.01722690313387
14-15	21.480958063304158	27.26423389284455	26.277309217172718	24.97749882667857
16-17	20.95023910317648	30.493679445306178	27.086138631700457	21.469942819816882
18-19	20.83566105433273	29.49194224023301	29.008354627611038	20.66404207782323
20-21	22.000009151937608	28.720677334902383	28.503452282464643	20.77586123069537
22-23	21.698786839591964	28.569434733640296	28.46881946852946	21.26295895823828
24-25	21.493689906395566	28.69735793340204	28.438353479448235	21.370598680754156
26-27	21.262288997975073	28.66857220592751	28.512000597915932	21.557138198181487
28-29	21.295887783468004	28.656274905335895	28.664654537966694	21.383182773229407
30-31	21.569774979694404	28.951058750853214	28.253490083624726	21.22567618582765
32-33	21.25676459325117	28.719645664821275	28.581567741168527	21.442022000759028
34-35	21.467522436698587	28.389881001748947	28.624462736614674	21.51813382493779
36-37	21.650268789938128	28.599274202343267	28.2305698316183	21.51988717610031
38-39	21.424386475375222	28.649779665550746	28.445002946626495	21.480830912447537
40-41	21.330430971885296	28.365174751323874	28.739632000422503	21.564762276368324
42-43	21.125601294964852	28.352399364719833	28.758362838016904	21.76363650229841
44-45	21.167023230035138	28.439323947464402	28.69889610574079	21.694756716759674
46-47	21.142496039348462	28.538159003178915	28.58688128493364	21.732463672538984
48-49	21.006340581696154	28.44851886745598	28.570014986586813	21.975125564261056
50-51	21.01606879493047	28.355773363842506	28.54801434400268	22.080143497224345
52-53	20.916140811337083	28.335920267640454	28.659812959025132	22.088125961997328
54-55	21.04281568146319	28.555843608629772	28.478874810354466	21.922465899552574
56-57	21.023969565313546	28.339241741003168	28.425684852894186	22.211103840789097
58-59	20.987389754969524	27.941318061452947	28.580038097082927	22.491254086494603
60-61	21.11980861218477	28.197786983058528	28.268358189434355	22.41404621532235
62-63	21.0791147445968	28.16399874929457	28.248726415813795	22.508160090294833
64-65	21.200562876749363	27.942987090287154	28.258103888547197	22.598346144416283
66-67	21.177112190395626	27.997185585937007	28.06139739032779	22.764304833339576
68-69	21.557329698194504	28.088158471716458	27.897599664448723	22.45691216564031
70-71	21.539430955729934	27.974589241514213	27.93593354461301	22.55004625814284
72-73	21.50655725328225	27.901118435501974	28.069329884328177	22.5229944268876
74-75	21.660698308402733	27.867466794632236	27.99722448939127	22.47461040757376
76-77	21.76141482551043	27.942048846537315	27.84367209159181	22.452864236360448
78-79	21.956502715196194	27.95186906506614	27.893891242298302	22.197736977439362
80-81	21.948610341610134	27.75948875732064	27.974374950191628	22.317525950877602
82-83	21.86028022883878	27.757592569555555	28.098343669017872	22.28378353258779
84-85	21.98205870728662	27.559008805730752	28.014042420869583	22.444890066113047
86-87	22.23655411717603	27.555289698957118	27.91069760347753	22.297458580389314
88-89	22.239768864769722	27.397385505774185	27.94896081423539	22.4138848152207
90-91	22.46270665722536	27.243758767810643	27.860036476380152	22.433498098583843
92-93	22.58439149895927	27.243062483288043	27.760667172215708	22.41187884553697
94-95	22.493988149223163	26.96753343195317	27.915387764136785	22.623090654686877
96-97	22.599319935725333	26.911825498579432	27.81156414211403	22.677290423581205
98-99	22.674726984173574	26.95624256578748	27.755835541163027	22.613194908875922
100	22.72720602067104	26.98272508665664	27.72980090747661	22.560267985195708
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1774.0
1	1424.0
2	1352.5
3	1639.5
4	1559.5
5	1359.0
6	1145.5
7	1004.0
8	964.5
9	976.5
10	943.0
11	916.0
12	908.5
13	937.0
14	1016.0
15	1164.5
16	1408.5
17	1727.0
18	2248.5
19	3065.5
20	4365.0
21	6194.0
22	8816.5
23	12716.0
24	18187.5
25	25751.5
26	35757.0
27	47355.0
28	60858.0
29	76497.5
30	92199.5
31	105738.5
32	116946.5
33	127580.0
34	138451.5
35	148286.5
36	157299.5
37	167270.0
38	177102.5
39	183290.5
40	187896.5
41	191840.0
42	192926.0
43	193950.0
44	193971.5
45	191750.0
46	188942.0
47	184291.5
48	178045.0
49	172751.0
50	167528.5
51	160657.0
52	151524.5
53	142404.0
54	134614.5
55	127029.0
56	116079.0
57	103077.0
58	91504.0
59	80555.0
60	69160.0
61	57790.5
62	48353.5
63	39851.5
64	31787.5
65	25267.5
66	20302.0
67	15611.0
68	11915.5
69	9358.5
70	7163.5
71	5449.5
72	4225.0
73	3103.0
74	2254.0
75	1699.5
76	1224.0
77	898.0
78	643.0
79	448.5
80	343.0
81	243.5
82	170.0
83	127.5
84	109.5
85	93.0
86	71.5
87	56.0
88	42.5
89	35.5
90	31.0
91	24.0
92	18.0
93	19.0
94	24.5
95	25.5
96	18.0
97	20.5
98	36.5
99	49.0
100	87.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015342518387674755
2	0.03760346432159291
3	0.038785124122879655
4	0.04191080488757365
5	0.044445655751624256
6	0.04286375634022425
7	0.04084349926060497
8	0.04030984644712063
9	0.04076726314439292
10-11	0.041262797899771234
12-13	0.0421490427507363
14-15	0.04060526139744232
16-17	0.037079341022635076
18-19	0.04000490198227243
20-21	0.039375954023523035
22-23	0.040519495766703766
24-25	0.037479580632748324
26-27	0.0375939348070664
28-29	0.0380608810188652
30-31	0.0380608810188652
32-33	0.037975115388126646
34-35	0.037927467815494116
36-37	0.03562132530007965
38-39	0.035754738503450734
40-41	0.036831573644945916
42-43	0.03672674898515435
44-45	0.03583097461966278
46-47	0.0347541394781676
48-49	0.03494472976869772
50-51	0.03578332704703025
52-53	0.03640274549125314
54-55	0.035726149959871216
56-57	0.03409660297583868
58-59	0.03499237734133025
60-61	0.034306252295421814
62-63	0.03463025578932302
64-65	0.031618929198947106
66-67	0.03269576434044229
68-69	0.03348671404614229
70-71	0.03496378879775073
72-73	0.03188575560568927
74-75	0.03232411327390855
76-77	0.03269576434044229
78-79	0.03331518278466518
80-81	0.03344859598803627
82-83	0.03200963929453385
84-85	0.0333533008427712
86-87	0.03348671404614229
88-89	0.03409660297583868
90-91	0.034668373847429046
92-93	0.03305788589244952
94-95	0.034306252295421814
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5246857.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.2648003353379
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.68599655305991	51.35425854222541
2	13.100388792019094	17.099885176528492
3	3.792603237876191	7.4257047921343675
4	1.601677471298239	4.181326414635538
5	0.8064206413237182	2.63154410711438
6	0.4799078694846547	1.879265476876403
7	0.2986292834866589	1.3642986392729268
8	0.21995644098177247	1.148433056251753
9	0.16190481854694294	0.9510017090215808
>10	0.8033568502615326	9.09712612099359
>50	0.03844952888242047	1.7004812227122783
>100	0.010562495937783287	1.0550434890924623
>500	5.840726258900469E-5	0.02121343343254054
>1k	8.76095785540794E-5	0.09041781970833802
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9059029053012117E-5	0.0	0.0	0.0
3	0.0	1.9059029053012117E-5	0.0	1.9059029053012117E-5	0.0
4	0.0	1.9059029053012117E-5	0.0	1.9059029053012117E-5	0.0
5	0.0	1.9059029053012117E-5	0.0	1.9059029053012117E-5	0.0
6	0.0	1.9059029053012117E-5	0.0	3.8118058106024234E-5	0.0
7	0.0	1.9059029053012117E-5	0.0	3.8118058106024234E-5	0.0
8	0.0	1.9059029053012117E-5	0.0	3.8118058106024234E-5	0.0
9	0.0	1.9059029053012117E-5	0.0	5.7177087159036354E-5	0.0
10-11	0.0	1.9059029053012117E-5	0.0	1.429427178975909E-4	0.0
12-13	0.0	1.9059029053012117E-5	0.0	1.9059029053012118E-4	0.0
14-15	0.0	1.9059029053012117E-5	0.0	2.3823786316265146E-4	0.0
16-17	0.0	1.9059029053012117E-5	0.0	3.049444648481939E-4	0.0
18-19	0.0	1.9059029053012117E-5	0.0	3.430625229542181E-4	0.0
20-21	0.0	1.9059029053012117E-5	0.0	6.48006987802412E-4	0.0
22-23	0.0	1.9059029053012117E-5	0.0	0.002067904652251815	0.0
24-25	0.0	1.9059029053012117E-5	0.0	0.00495534755378315	0.0
26-27	0.0	1.9059029053012117E-5	0.0	0.010463406950103653	1.9059029053012117E-5
28-29	0.0	1.9059029053012117E-5	0.0	0.030456328426713364	1.9059029053012117E-5
30-31	0.0	1.9059029053012117E-5	0.0	0.09226475964563166	1.9059029053012117E-5
32-33	0.0	1.9059029053012117E-5	0.0	0.22881317329593698	1.9059029053012117E-5
34-35	0.0	1.9059029053012117E-5	0.0	0.4350032791059486	1.9059029053012117E-5
36-37	0.0	1.9059029053012117E-5	0.0	0.7504492689623521	1.9059029053012117E-5
38-39	0.0	1.9059029053012117E-5	0.0	1.1499836950006452	1.9059029053012117E-5
40-41	0.0	3.8118058106024234E-5	0.0	1.5414656812640404	1.9059029053012117E-5
42-43	0.0	3.8118058106024234E-5	0.0	1.9194538749579033	1.9059029053012117E-5
44-45	0.0	3.8118058106024234E-5	0.0	2.373859245639818	1.9059029053012117E-5
46-47	0.0	3.8118058106024234E-5	0.0	2.892245776852695	1.9059029053012117E-5
48-49	0.0	3.8118058106024234E-5	0.0	3.4046573024574522	1.9059029053012117E-5
50-51	0.0	3.8118058106024234E-5	0.0	3.8937501060158493	1.9059029053012117E-5
52-53	0.0	3.8118058106024234E-5	0.0	4.347917238834601	1.9059029053012117E-5
54-55	0.0	3.8118058106024234E-5	0.0	4.827185112916171	1.9059029053012117E-5
56-57	0.0	3.8118058106024234E-5	0.0	5.369528843648683	1.9059029053012117E-5
58-59	0.0	3.8118058106024234E-5	0.0	5.933714221675948	1.9059029053012117E-5
60-61	0.0	5.7177087159036354E-5	0.0	6.492477305937631	1.9059029053012117E-5
62-63	0.0	5.7177087159036354E-5	0.0	7.014580347815845	1.9059029053012117E-5
64-65	0.0	5.7177087159036354E-5	0.0	7.551539902840881	1.9059029053012117E-5
66-67	0.0	7.623611621204847E-5	0.0	8.13660444719572	1.9059029053012117E-5
68-69	0.0	7.623611621204847E-5	0.0	8.753621453757937	1.9059029053012117E-5
70-71	0.0	8.576563073855453E-5	0.0	9.372172712158918	1.9059029053012117E-5
72-73	0.0	9.529514526506059E-5	0.0	9.974895446931374	1.9059029053012117E-5
74-75	0.0	1.1435417431807271E-4	0.0	10.554937937130743	1.9059029053012117E-5
76-77	0.0	1.1435417431807271E-4	0.0	11.158156206658576	1.9059029053012117E-5
78-79	1.9059029053012117E-5	1.1435417431807271E-4	0.0	11.801388907683208	1.9059029053012117E-5
80-81	1.9059029053012117E-5	1.1435417431807271E-4	0.0	12.464433469408448	1.9059029053012117E-5
82-83	1.9059029053012117E-5	1.1435417431807271E-4	0.0	13.109638779940067	1.9059029053012117E-5
84-85	1.9059029053012117E-5	1.3341320337108482E-4	0.0	13.73268796919756	1.9059029053012117E-5
86-87	1.9059029053012117E-5	1.3341320337108482E-4	0.0	14.362960530466143	1.9059029053012117E-5
88	1.9059029053012117E-5	1.3341320337108482E-4	0.0	14.858857407396465	1.9059029053012117E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5530	0.0	46.842644	1
GTATCAA	9125	0.0	35.80691	1
TCAACGC	9985	0.0	31.497826	4
ATCAACG	10080	0.0	31.294247	3
CAACGCA	10700	0.0	29.437561	5
AACGCAG	10985	0.0	28.669172	6
TATCAAC	11450	0.0	27.837276	2
ACGCAGA	12885	0.0	24.076406	7
CGCAGAG	13060	0.0	23.537846	8
GTACATG	14825	0.0	23.086157	1
TACATGG	14650	0.0	21.917227	2
ACATGGG	15270	0.0	20.503958	3
CGTATCA	1390	0.0	19.948551	94
GTGGTAT	3215	0.0	19.740936	1
GCCGTAT	2115	0.0	19.33228	94
GCAGAGT	15075	0.0	19.269365	9
CGCCGTA	2510	0.0	18.91132	94
CATGGGG	10660	0.0	17.816677	4
GAGTACA	11510	0.0	17.685913	1
AGAGTAC	12920	0.0	17.553797	10-11
>>END_MODULE