FastQCFastQC Report
Wed 25 May 2016
SRR2049346_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049346_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5246857
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148080.28222610221700345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145410.2771373414598492No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT140220.2672457053813359No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80480.15338706581864153No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT67160.1280004391200294RNA PCR Primer, Index 46 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93800.058.740871
GTATCAA171700.040.7425541
ATCAACG212750.032.4938663
TCAACGC212950.032.4633524
CAACGCA220850.031.3446665
TATCAAC222250.031.2740942
AACGCAG227350.030.5355536
GTGGTAT40500.029.4845371
TGGTATC40800.028.5660272
ACGCAGA261150.026.3315167
CGCAGAG264800.025.8442258
GCAGAGT284600.023.5667159
GTACATG140450.022.995911
GAGTACT200650.022.1660512-13
TACATGG143150.021.2079892
CAGAGTA277450.021.06328210-11
AGAGTAC264700.020.30984710-11
GTACTTT217900.020.25213414-15
ACATGGG145150.019.912073
AGTACTT210700.019.31251512-13
Produced by FastQC (version 0.11.5)