##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049346_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5246857
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76786426616925	31.0	31.0	34.0	30.0	34.0
2	31.949874372409997	33.0	31.0	34.0	30.0	34.0
3	31.92364781430102	33.0	31.0	34.0	30.0	34.0
4	35.62997733690855	37.0	35.0	37.0	33.0	37.0
5	35.73896486982588	37.0	35.0	37.0	33.0	37.0
6	35.79040480805938	37.0	35.0	37.0	35.0	37.0
7	35.73003800179803	37.0	35.0	37.0	33.0	37.0
8	35.73541588802592	37.0	35.0	37.0	33.0	37.0
9	37.468915771861134	39.0	37.0	39.0	34.0	39.0
10-11	37.43146801980691	39.0	37.0	39.0	34.0	39.0
12-13	37.375073496380786	39.0	37.0	39.0	34.0	39.0
14-15	38.63534464537532	40.0	38.0	41.0	34.0	41.0
16-17	38.66358212163968	40.0	38.0	41.0	34.0	41.0
18-19	38.74002722010529	40.0	38.0	41.0	34.0	41.0
20-21	38.776572527133865	40.0	38.0	41.0	34.0	41.0
22-23	38.739605920268076	40.0	38.0	41.0	34.0	41.0
24-25	38.7137235682238	40.0	38.0	41.0	34.0	41.0
26-27	38.59255493336296	40.0	38.0	41.0	34.0	41.0
28-29	38.50636342862022	40.0	38.0	41.0	34.0	41.0
30-31	38.394997424172224	40.0	38.0	41.0	34.0	41.0
32-33	38.2394000827543	40.0	38.0	41.0	34.0	41.0
34-35	38.126610368836054	40.0	38.0	41.0	33.5	41.0
36-37	37.99447097567172	40.0	37.5	41.0	33.0	41.0
38-39	37.88603786990954	40.0	37.0	41.0	33.0	41.0
40-41	37.78531976381289	40.0	37.0	41.0	33.0	41.0
42-43	37.648507096724764	40.0	37.0	41.0	33.0	41.0
44-45	37.46548629017333	40.0	37.0	41.0	32.5	41.0
46-47	37.225277437521164	40.0	37.0	41.0	32.0	41.0
48-49	37.007952665757806	40.0	37.0	41.0	31.0	41.0
50-51	36.84471903846436	40.0	36.0	41.0	31.0	41.0
52-53	36.71504350128086	40.0	36.0	41.0	31.0	41.0
54-55	36.57581653168745	39.5	36.0	41.0	31.0	41.0
56-57	36.43000609698339	39.0	35.0	41.0	30.5	41.0
58-59	36.24069466730273	39.0	35.0	41.0	30.0	41.0
60-61	36.05696505546082	39.0	35.0	41.0	30.0	41.0
62-63	35.849025997849765	39.0	35.0	41.0	29.5	41.0
64-65	35.60197285727436	38.0	35.0	40.0	29.0	41.0
66-67	35.2972682503068	37.5	35.0	40.0	29.0	41.0
68-69	34.96145578200435	37.0	34.5	40.0	28.5	41.0
70-71	34.59761329496878	36.5	34.0	39.5	28.0	41.0
72-73	34.29182260923063	36.0	34.0	39.0	28.0	41.0
74-75	33.97476737025614	36.0	34.0	39.0	28.0	40.5
76-77	33.63004480587141	35.0	34.0	38.5	27.0	40.0
78-79	33.27830804231943	35.0	34.0	37.0	26.5	39.0
80-81	32.94399885493354	35.0	33.0	37.0	26.0	39.0
82-83	32.631445453916506	35.0	33.0	36.0	26.0	39.0
84-85	32.35404919554698	35.0	33.0	36.0	26.0	37.0
86-87	32.10590416319713	35.0	33.0	35.5	26.0	37.0
88-89	31.859951395664112	35.0	33.0	35.0	25.0	37.0
90-91	31.65009509502546	35.0	33.0	35.0	25.0	36.0
92-93	31.454136543077123	35.0	33.0	35.0	25.0	36.0
94-95	31.283117111825234	35.0	33.0	35.0	24.0	36.0
96-97	31.06825791135531	35.0	33.0	35.0	23.5	35.5
98-99	30.894973219205326	35.0	32.5	35.0	21.5	35.0
100	30.763971268894885	35.0	32.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.14719241627355828
1101	2	0.21745375211099827
1101	3	0.3768272108901449
1101	4	0.20104126014462054
1101	5	0.16944499968899862
1101	6	0.1829552157872243
1101	7	0.10088026297432862
1101	8	0.17130436709525299
1101	9	0.2700747114721267
1101	10-11	0.25517192520290166
1101	12-13	0.17377432381426416
1101	14-15	0.3174935723839667
1101	16-17	0.23631140246990867
1101	18-19	0.3164492560141383
1101	20-21	0.33722025884005546
1101	22-23	0.3074945275742422
1101	24-25	0.24934546408681513
1101	26-27	0.3436509605338003
1101	28-29	0.41063782065933196
1101	30-31	0.33711009654688695
1101	32-33	0.35250068973708437
1101	34-35	0.3587829590746239
1101	36-37	0.3650868957588216
1101	38-39	0.3887069287421028
1101	40-41	0.36542595020831925
1101	42-43	0.4376276935360721
1101	44-45	0.3947600664217319
1101	46-47	0.37689349320193344
1101	48-49	0.3789005923027844
1101	50-51	0.30304947247125114
1101	52-53	0.36230925358739086
1101	54-55	0.3740331873628051
1101	56-57	0.36313724182239326
1101	58-59	0.3773374586907323
1101	60-61	0.3594979028396139
1101	62-63	0.3112650966880892
1101	64-65	0.24542024097236492
1101	66-67	0.2379624792244286
1101	68-69	0.2964292963343311
1101	70-71	0.3146283824340017
1101	72-73	0.35056828616211533
1101	74-75	0.37448155458942267
1101	76-77	0.35271108539085816
1101	78-79	0.3491065288005899
1101	80-81	0.39906702853424036
1101	82-83	0.4427320279053859
1101	84-85	0.4338226181955207
1101	86-87	0.39701302783943504
1101	88-89	0.46905846206223956
1101	90-91	0.488453719944669
1101	92-93	0.4977174421639141
1101	94-95	0.520970923037595
1101	96-97	0.5675794876228508
1101	98-99	0.5783595458573174
1101	100	0.6076380646775341
1102	1	0.25637764707367694
1102	2	0.2636303964363549
1102	3	0.4033419283549726
1102	4	0.2221327925832739
1102	5	0.22870854732386192
1102	6	0.1773491048734428
1102	7	0.1716890478954909
1102	8	0.21993433404659157
1102	9	0.16200062610156607
1102	10-11	0.26063562958725583
1102	12-13	0.20262991737749303
1102	14-15	0.2885947279288814
1102	16-17	0.26247682144663287
1102	18-19	0.28548707086441993
1102	20-21	0.27297622767547125
1102	22-23	0.2785441893659808
1102	24-25	0.2610730481369714
1102	26-27	0.31065794283577475
1102	28-29	0.29000079418951685
1102	30-31	0.2759916058978007
1102	32-33	0.28944718256366997
1102	34-35	0.37422411624108776
1102	36-37	0.3158744075343378
1102	38-39	0.30200071435821485
1102	40-41	0.29766662824639667
1102	42-43	0.2915298389965102
1102	44-45	0.33376079804806835
1102	46-47	0.33108251726259397
1102	48-49	0.29266838105388615
1102	50-51	0.41143820542570353
1102	52-53	0.3881942318895568
1102	54-55	0.3405667746097336
1102	56-57	0.41958980000502777
1102	58-59	0.384139238144968
1102	60-61	0.36356658498561956
1102	62-63	0.3695495777159934
1102	64-65	0.3380209795481761
1102	66-67	0.3590212622151725
1102	68-69	0.39970789925619954
1102	70-71	0.42911202604897625
1102	72-73	0.3496921105409072
1102	74-75	0.3707215550527181
1102	76-77	0.3739287027213223
1102	78-79	0.4005082013099681
1102	80-81	0.3947660695053159
1102	82-83	0.38550218913343315
1102	84-85	0.40815459895316764
1102	86-87	0.391244936013571
1102	88-89	0.4130346774499465
1102	90-91	0.45789424779665566
1102	92-93	0.473653615005329
1102	94-95	0.471096525326292
1102	96-97	0.5295532282217437
1102	98-99	0.5535484753277657
1102	100	0.6151731981848627
1103	1	0.17515600523474362
1103	2	0.14661434833529086
1103	3	0.3319764721189422
1103	4	0.13565365793642314
1103	5	0.15956807944805007
1103	6	0.10289676606331
1103	7	0.11140970234452396
1103	8	0.09900202316281792
1103	9	0.17098565118113385
1103	10-11	0.17460188765943485
1103	12-13	0.1773694298585724
1103	14-15	0.25853204380307915
1103	16-17	0.25645534964174743
1103	18-19	0.277905645900816
1103	20-21	0.24012411132959244
1103	22-23	0.21562633956848032
1103	24-25	0.181823546383157
1103	26-27	0.2338351400684502
1103	28-29	0.3433753738262979
1103	30-31	0.25452865770948563
1103	32-33	0.3079154652516465
1103	34-35	0.34395909341893116
1103	36-37	0.38189435922755877
1103	38-39	0.3174881946001733
1103	40-41	0.3378325934922728
1103	42-43	0.36600063760583623
1103	44-45	0.42611840477332663
1103	46-47	0.35730857607271105
1103	48-49	0.33106946934461234
1103	50-51	0.3672406764719014
1103	52-53	0.39881261240628163
1103	54-55	0.33951796127534806
1103	56-57	0.3516824339716891
1103	58-59	0.40010383319678056
1103	60-61	0.39111683400630426
1103	62-63	0.3820166870962254
1103	64-65	0.4082442038506713
1103	66-67	0.36488250332718764
1103	68-69	0.37006137073157674
1103	70-71	0.40937096387321503
1103	72-73	0.43423004438145796
1103	74-75	0.365267976941972
1103	76-77	0.3950903502128895
1103	78-79	0.37857995331148686
1103	80-81	0.45602907294559003
1103	82-83	0.44541404086156433
1103	84-85	0.4606363177898558
1103	86-87	0.41277940227004706
1103	88-89	0.4121764151196281
1103	90-91	0.3946158311522474
1103	92-93	0.47566299327510464
1103	94-95	0.3715449946785192
1103	96-97	0.47750261123390203
1103	98-99	0.5993457716225592
1103	100	0.6108791831011686
1104	1	0.1975671106700645
1104	2	0.19253931598263563
1104	3	0.29757599562563897
1104	4	0.21204473683120995
1104	5	0.16030246516871927
1104	6	0.20534557788906227
1104	7	0.22222573324332018
1104	8	0.16744819304381053
1104	9	0.23390018364375464
1104	10-11	0.22945638332829787
1104	12-13	0.2128259308990721
1104	14-15	0.2745027789354495
1104	16-17	0.2781412455229244
1104	18-19	0.291438694126839
1104	20-21	0.2979022609317923
1104	22-23	0.2755017119191763
1104	24-25	0.3171366395068631
1104	26-27	0.3616917163965141
1104	28-29	0.3861315884345302
1104	30-31	0.3650717858039485
1104	32-33	0.40321425456354376
1104	34-35	0.3511008685628738
1104	36-37	0.3738814588618169
1104	38-39	0.417537142181601
1104	40-41	0.4345061737883853
1104	42-43	0.43964038873856737
1104	44-45	0.2936979884959712
1104	46-47	0.29182402038656363
1104	48-49	0.29774098754023726
1104	50-51	0.32370638830239784
1104	52-53	0.2668318351249397
1104	54-55	0.24944576849473066
1104	56-57	0.2767599717188034
1104	58-59	0.34050356524239334
1104	60-61	0.33963406783603034
1104	62-63	0.38321732011583265
1104	64-65	0.2648290630864665
1104	66-67	0.33386086850652674
1104	68-69	0.2970648972519143
1104	70-71	0.3439278256359657
1104	72-73	0.30965761547150805
1104	74-75	0.3621014942945635
1104	76-77	0.32488193496127593
1104	78-79	0.30827227722201656
1104	80-81	0.3216531184028284
1104	82-83	0.3725442105198482
1104	84-85	0.384198618465156
1104	86-87	0.40919899453280806
1104	88-89	0.3987290139811286
1104	90-91	0.313916881774297
1104	92-93	0.39658092399812617
1104	94-95	0.4781892151977445
1104	96-97	0.5267992309801137
1104	98-99	0.5231106617587358
1104	100	0.48576541196624845
1105	1	0.18503061713607138
1105	2	0.18764597013766604
1105	3	0.3102982826025773
1105	4	0.1712272195095892
1105	5	0.11080298939968714
1105	6	0.14017089680135086
1105	7	0.12085376857090324
1105	8	0.16219485808995415
1105	9	0.15689714409378297
1105	10-11	0.19192409582873182
1105	12-13	0.1864403666471759
1105	14-15	0.23911810038403303
1105	16-17	0.24317361256296266
1105	18-19	0.19606969956495846
1105	20-21	0.23464687289331465
1105	22-23	0.19019607082751833
1105	24-25	0.24201348673707201
1105	26-27	0.25504255871982906
1105	28-29	0.24916765743918035
1105	30-31	0.19676262963709235
1105	32-33	0.23425905805197544
1105	34-35	0.18487496104296497
1105	36-37	0.17293592105744438
1105	38-39	0.16354908134871948
1105	40-41	0.19830771243225342
1105	42-43	0.16467078791883694
1105	44-45	0.19539625310702746
1105	46-47	0.21935558077585426
1105	48-49	0.24849828230068738
1105	50-51	0.24109303455000486
1105	52-53	0.2537313362978537
1105	54-55	0.17276494757823713
1105	56-57	0.1751770060232758
1105	58-59	0.210618555810818
1105	60-61	0.1866189525301607
1105	62-63	0.2505755894330619
1105	64-65	0.21916823066869995
1105	66-67	0.25948695830292223
1105	68-69	0.20559655897861973
1105	70-71	0.2427459401858485
1105	72-73	0.30454162175262667
1105	74-75	0.23010393097278126
1105	76-77	0.20398655222604134
1105	78-79	0.23613574832215534
1105	80-81	0.24312743304822249
1105	82-83	0.25669456416616754
1105	84-85	0.2655470211658155
1105	86-87	0.2627816811619397
1105	88-89	0.2665822826362749
1105	90-91	0.35149630466792914
1105	92-93	0.43075334859029013
1105	94-95	0.3494661345337349
1105	96-97	0.4475778180486252
1105	98-99	0.4562161317627371
1105	100	0.4756324881920264
1106	1	0.11732710866192164
1106	2	0.13833263071641966
1106	3	0.23208170827568964
1106	4	0.1741302838104417
1106	5	0.07873504347113425
1106	6	0.12083840110224031
1106	7	0.133771176165439
1106	8	0.1244991517414391
1106	9	0.14997155395180073
1106	10-11	0.1755255282449184
1106	12-13	0.13528001292446845
1106	14-15	0.18901110082035188
1106	16-17	0.18366868427428784
1106	18-19	0.17360421047192176
1106	20-21	0.21027430997054353
1106	22-23	0.20775702784926153
1106	24-25	0.20569740238114065
1106	26-27	0.2845543657495142
1106	28-29	0.19712258583457043
1106	30-31	0.2442870062398299
1106	32-33	0.22879073168579822
1106	34-35	0.2986936805630691
1106	36-37	0.29771388777243146
1106	38-39	0.32812743887359375
1106	40-41	0.34639101342472145
1106	42-43	0.3122661655250383
1106	44-45	0.36622043790980996
1106	46-47	0.44474376399844573
1106	48-49	0.4332841452190266
1106	50-51	0.40205669707284386
1106	52-53	0.4298983762580306
1106	54-55	0.32529932606488643
1106	56-57	0.3422366829951926
1106	58-59	0.39391033815419974
1106	60-61	0.38836365138937623
1106	62-63	0.4253984011755705
1106	64-65	0.3743525885966079
1106	66-67	0.4013867762902166
1106	68-69	0.40098239500180455
1106	70-71	0.4589520832707805
1106	72-73	0.5262037504213666
1106	74-75	0.4327979134855511
1106	76-77	0.36760340390035395
1106	78-79	0.49329467095684265
1106	80-81	0.4863562766109837
1106	82-83	0.46817466194691093
1106	84-85	0.4240804982456865
1106	86-87	0.43362716389405165
1106	88-89	0.4290869855337185
1106	90-91	0.42808507367054105
1106	92-93	0.4693241436900557
1106	94-95	0.4207103249384865
1106	96-97	0.4959152395461075
1106	98-99	0.4973504575816854
1106	100	0.5361552108453154
1107	1	0.14187632452156507
1107	2	0.12062702589055618
1107	3	0.23006940201529247
1107	4	0.16100318247629986
1107	5	0.15842233728943
1107	6	0.17302784879893096
1107	7	0.10256846550590382
1107	8	0.13520589737319
1107	9	0.15294143049325015
1107	10-11	0.17883479511733213
1107	12-13	0.1269504098292913
1107	14-15	0.173321552509897
1107	16-17	0.16021539521153016
1107	18-19	0.1844482045896001
1107	20-21	0.10650117374461843
1107	22-23	0.14777753337588706
1107	24-25	0.1746473751414257
1107	26-27	0.27488158247373917
1107	28-29	0.28031911880129456
1107	30-31	0.20226374830495786
1107	32-33	0.22946449134511937
1107	34-35	0.24846432224952508
1107	36-37	0.25354412798479586
1107	38-39	0.22241765235841626
1107	40-41	0.24242370077146802
1107	42-43	0.2991734053691104
1107	44-45	0.3123093209684171
1107	46-47	0.38504323735881485
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2216	90-91	-1.2508940513167808
2216	92-93	-1.3776763052270304
2216	94-95	-1.3557795391278233
2216	96-97	-1.4071441837448688
2216	98-99	-1.573443675119428
2216	100	-1.5456650988149718
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	261.0
3	29.0
4	44.0
5	92.0
6	299.0
7	787.0
8	1838.0
9	3305.0
10	4758.0
11	6786.0
12	9532.0
13	12842.0
14	15126.0
15	19641.0
16	24396.0
17	32952.0
18	33432.0
19	19541.0
20	15594.0
21	16223.0
22	17660.0
23	20134.0
24	24603.0
25	30075.0
26	35859.0
27	39885.0
28	51175.0
29	66192.0
30	85858.0
31	111220.0
32	143773.0
33	185626.0
34	251976.0
35	362543.0
36	577677.0
37	1033971.0
38	1427107.0
39	561962.0
40	2083.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.69714522568734	20.981793285061983	15.081393794734844	23.23966769451583
2	15.28320668926178	20.666753448778955	40.42776465987161	23.62227520208765
3	17.50970152226371	25.78071024234127	31.408669990434273	25.300918244960748
4	12.480500230900136	18.040343009157674	38.16907912679915	31.31007763314304
5	12.456047496625121	37.87545572520845	35.92102853193826	13.74746824622817
6	29.89233363897663	38.71275698956537	17.32890757266684	14.066001798791161
7	25.77514500585779	33.4662255899103	22.550643175523938	18.207986228707966
8	24.315067253001764	36.76252837666331	20.430663930537854	18.491740439797066
9	24.337484326330983	16.796398300925677	21.826971842381067	37.03914553036227
10-11	23.424756954496758	27.332782273273317	28.847917905900616	20.39454286632931
12-13	24.27839387430741	24.775787970464865	29.17371011168105	21.772108043546677
14-15	21.546724509565777	26.803682701000632	26.833729268892576	24.81586352054102
16-17	20.487667234657554	30.208411455506738	27.606910918947374	21.697010390888334
18-19	20.295783127822766	28.34672644601495	30.672807365789502	20.684683060372784
20-21	21.34742686210447	27.448942008566092	30.55888268252252	20.64474844680692
22-23	21.043140684032362	27.52266737972847	30.54984155276197	20.884350383477194
24-25	21.02466393182045	27.66788542920593	30.51212422229558	20.79532641667804
26-27	20.79491428674892	27.638185160443733	30.643290123321492	20.923610429485855
28-29	20.682477453095576	27.49001303641811	30.91029076548932	20.917218744996987
30-31	21.02847476117607	27.968105477240947	30.359689619900067	20.643730141682916
32-33	21.00329216797175	27.513316808486778	30.6802571792409	20.80313384430057
34-35	20.95596759313657	27.23241738402545	30.688575805890444	21.123039216947532
36-37	21.07517391137374	27.657318810216445	30.266501628070998	21.001005650338815
38-39	20.938496224537413	27.419758614732046	30.528448501531543	21.113296659198998
40-41	20.78814747444561	27.226670183698857	30.731355282257084	21.25382705959845
42-43	20.867222850220582	27.182103704923488	30.61563470050196	21.33503874435397
44-45	20.774061585789628	27.4741661343625	30.35037160913986	21.401400670708018
46-47	20.99711407907065	27.47040511148399	29.93573098688509	21.59674982256027
48-49	20.918645455564256	27.507189127416805	29.655922491600613	21.91824292541833
50-51	20.804450836447273	27.509389471822438	29.61359173932318	22.07256795240711
52-53	20.711508795228806	27.578808657888526	29.757203503377855	21.95247904350481
54-55	20.77559533997073	27.80955143616131	29.403407845569348	22.011445378298607
56-57	20.803323532172538	27.580809546340145	29.250094461843844	22.36577245964347
58-59	20.538616195269814	27.46415803911411	29.564502760035396	22.43272300558068
60-61	20.788457986879365	27.9343754699249	28.93863536000369	22.338531183192046
62-63	20.47878684208144	28.044852078956662	28.94046421606302	22.53589686289888
64-65	20.46200082125771	27.824998349003273	29.000193545417563	22.71280728432146
66-67	20.535952697745984	27.928368868536047	28.74385169234628	22.79182674137169
68-69	20.873364001792726	28.0476602839639	28.488427664433193	22.590548049810184
70-71	20.86476977527383	27.83311065919803	28.451760396277372	22.850359169250765
72-73	20.68389862699195	27.943083795249713	28.473597531143263	22.89942004661507
74-75	20.94904068734421	27.848736854734508	28.27338588151815	22.92883657640314
76-77	21.038704334086074	27.850464724745894	28.106248447272446	23.004582493895583
78-79	21.067995204197558	27.929821792730873	28.13600012884359	22.866182874227974
80-81	20.918442856079604	27.8431919672106	28.205505732131776	23.032859444578023
82-83	20.87543300408346	27.74184141687036	28.25290773856321	23.129817840482964
84-85	21.008558160881684	27.54115548704249	28.09366594697455	23.356620405101275
86-87	21.09363378256566	27.66405321526186	28.059601672687677	23.182711329484803
88-89	20.95321248881687	27.435339873773096	28.15856964188443	23.452877995525604
90-91	20.847448178066468	27.31049132381972	28.198555566197182	23.64350493191663
92-93	20.87468230060581	27.340969236882955	28.17775156026497	23.606596902246267
94-95	20.784982912385868	27.051159347517213	28.396811938467625	23.76704580162929
96-97	20.873448838287505	27.06201883193586	28.313764581265556	23.75076774851108
98-99	20.8737306629109	27.142783763196782	28.22884085581268	23.754644718079636
100	20.827831533622224	27.109489117872183	28.21514120692968	23.847538141575917
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	553.0
1	443.5
2	594.0
3	879.5
4	601.5
5	407.5
6	1042.0
7	2868.5
8	5053.0
9	5920.0
10	4544.5
11	2800.0
12	2491.5
13	2783.0
14	3207.0
15	3655.5
16	4267.5
17	4879.5
18	5544.5
19	6511.5
20	7814.5
21	9526.0
22	11992.0
23	15704.5
24	20788.0
25	27771.0
26	37106.0
27	48542.0
28	61520.5
29	76344.5
30	91996.5
31	105171.0
32	115722.0
33	126033.5
34	136539.0
35	146253.5
36	155495.5
37	165451.0
38	175475.5
39	182152.0
40	187589.5
41	192193.0
42	194647.5
43	197323.0
44	198072.5
45	196864.5
46	193590.5
47	187260.5
48	180194.5
49	174258.0
50	167822.5
51	159532.5
52	149146.5
53	139510.5
54	131509.0
55	122621.5
56	111285.0
57	98270.0
58	86847.5
59	76978.5
60	66127.0
61	54791.0
62	45716.5
63	37353.0
64	29170.5
65	23040.5
66	18079.0
67	13687.5
68	10277.0
69	7747.0
70	5732.0
71	4149.0
72	3029.5
73	2083.5
74	1472.0
75	1015.5
76	657.0
77	426.5
78	259.0
79	156.0
80	88.5
81	39.0
82	24.5
83	20.5
84	13.5
85	6.0
86	2.0
87	1.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036669571897995315
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	5.336528134843393E-4
9	0.0
10-11	0.0
12-13	0.044283654004673656
14-15	2.8588543579518177E-5
16-17	9.529514526506059E-6
18-19	1.1435417431807271E-4
20-21	8.576563073855453E-5
22-23	0.0
24-25	9.529514526506059E-5
26-27	3.8118058106024234E-5
28-29	3.24003493901206E-4
30-31	0.0
32-33	2.0011980505662722E-4
34-35	1.0482465979156665E-4
36-37	6.670660168554241E-5
38-39	3.1447397937469995E-4
40-41	1.7153126147710906E-4
42-43	5.336528134843393E-4
44-45	2.5729689221566356E-4
46-47	9.529514526506059E-5
48-49	8.576563073855452E-4
50-51	4.383576682192787E-4
52-53	6.670660168554242E-4
54-55	4.383576682192787E-4
56-57	5.622413570638574E-4
58-59	7.623611621204847E-5
60-61	1.5247223242409694E-4
62-63	0.0010387170833891604
64-65	5.050642699048211E-4
66-67	0.0016771945566650664
68-69	0.0018773143617216935
70-71	5.145937844313271E-4
72-73	0.0010673056269686785
74-75	0.0010196580543361483
76-77	7.909497057000028E-4
78-79	0.0035354498893337477
80-81	0.003078033192061457
82-83	0.0015628403823469937
84-85	0.0032209759099590483
86-87	0.0017439011583506086
88-89	0.0021917883410963934
90-91	0.0031352102792204933
92-93	0.002896972416057842
94-95	0.004202515906189172
96-97	0.0035163908602807354
98-99	0.0032686234825915783
100	0.0016962535857180786
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5246857.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.473802434952304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.45939654579517	48.39183036532635
2	13.659417287918302	17.0671147404396
3	4.153415562153976	7.784389897807915
4	1.6622825620802653	4.153964494978753
5	0.9117215809379636	2.847935696160036
6	0.5055765020644489	1.8951171903437185
7	0.34488403208158835	1.5082351818264434
8	0.23505465760078553	1.1747806592293468
9	0.1612579894092501	0.9066959794270012
>10	0.8490995797422136	9.294026360105988
>50	0.04305153777805357	1.8419867527594138
>100	0.01395721187867038	1.4663588319417604
>500	3.967027527098908E-4	0.19310160452202557
>1k	3.3567036710283667E-4	0.36616581345446286
>5k	6.1030975827590855E-5	0.2814636366609016
>10k+	9.154646374138628E-5	0.8268327950162531
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14808	0.28222610221700345	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14541	0.2771373414598492	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	14022	0.2672457053813359	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8048	0.15338706581864153	No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	6716	0.1280004391200294	RNA PCR Primer, Index 46 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	7.623611621204847E-5	0.0
12-13	0.0	0.0	0.0	1.1435417431807271E-4	0.0
14-15	0.0	0.0	0.0	2.1917883410963937E-4	1.9059029053012117E-5
16-17	0.0	0.0	0.0	2.858854357951818E-4	1.9059029053012117E-5
18-19	0.0	0.0	0.0	3.335330084277121E-4	1.9059029053012117E-5
20-21	0.0	0.0	0.0	6.289479587493999E-4	1.9059029053012117E-5
22-23	0.0	0.0	0.0	0.0020202570796192844	1.9059029053012117E-5
24-25	0.0	0.0	0.0	0.004974406582836163	1.9059029053012117E-5
26-27	0.0	0.0	0.0	0.010520584037262689	1.9059029053012117E-5
28-29	0.0	0.0	0.0	0.030770802406088066	1.9059029053012117E-5
30-31	0.0	0.0	0.0	0.09266499925574492	1.9059029053012117E-5
32-33	0.0	0.0	0.0	0.22977565426311408	1.9059029053012117E-5
34-35	0.0	0.0	0.0	0.43625164550892087	1.9059029053012117E-5
36-37	0.0	0.0	0.0	0.752631527788922	1.9059029053012117E-5
38-39	0.0	0.0	0.0	1.1528234903295438	1.9059029053012117E-5
40-41	0.0	0.0	0.0	1.545468077365173	1.9059029053012117E-5
42-43	0.0	0.0	0.0	1.924228161735683	1.9059029053012117E-5
44-45	0.0	0.0	0.0	2.379729426588146	1.9059029053012117E-5
46-47	0.0	0.0	0.0	2.8997931523576876	1.9059029053012117E-5
48-49	0.0	0.0	0.0	3.41426305310017	1.9059029053012117E-5
50-51	0.0	0.0	0.0	3.90514740538955	1.9059029053012117E-5
52-53	0.0	0.0	0.0	4.360048310826844	1.9059029053012117E-5
54-55	0.0	0.0	0.0	4.8410314975231845	1.9059029053012117E-5
56-57	0.0	0.0	0.0	5.385509839509634	1.9059029053012117E-5
58-59	0.0	0.0	0.0	5.951934653450627	1.9059029053012117E-5
60-61	0.0	0.0	0.0	6.511650689164961	1.9059029053012117E-5
62-63	0.0	0.0	0.0	7.036564937828494	1.9059029053012117E-5
64-65	0.0	0.0	0.0	7.576268993037165	1.9059029053012117E-5
66-67	0.0	0.0	0.0	8.164068508061112	1.9059029053012117E-5
68-69	0.0	0.0	0.0	8.784268372475179	1.9059029053012117E-5
70-71	0.0	0.0	0.0	9.40705073532593	1.9059029053012117E-5
72-73	0.0	0.0	0.0	10.01483364231196	1.9059029053012117E-5
74-75	0.0	0.0	0.0	10.59926923870805	1.9059029053012117E-5
76-77	1.9059029053012117E-5	0.0	0.0	11.206680494627545	1.9059029053012117E-5
78-79	1.9059029053012117E-5	0.0	0.0	11.855230664757968	1.9059029053012117E-5
80-81	1.9059029053012117E-5	0.0	0.0	12.523764226850474	1.9059029053012117E-5
82-83	1.9059029053012117E-5	0.0	0.0	13.17426794745883	1.9059029053012117E-5
84-85	1.9059029053012117E-5	0.0	0.0	13.800738232431339	1.9059029053012117E-5
86-87	1.9059029053012117E-5	0.0	0.0	14.43503224883011	1.9059029053012117E-5
88	1.9059029053012117E-5	0.0	0.0	14.936275183409801	1.9059029053012117E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9380	0.0	58.74087	1
GTATCAA	17170	0.0	40.742554	1
ATCAACG	21275	0.0	32.493866	3
TCAACGC	21295	0.0	32.463352	4
CAACGCA	22085	0.0	31.344666	5
TATCAAC	22225	0.0	31.274094	2
AACGCAG	22735	0.0	30.535553	6
GTGGTAT	4050	0.0	29.484537	1
TGGTATC	4080	0.0	28.566027	2
ACGCAGA	26115	0.0	26.331516	7
CGCAGAG	26480	0.0	25.844225	8
GCAGAGT	28460	0.0	23.566715	9
GTACATG	14045	0.0	22.99591	1
GAGTACT	20065	0.0	22.16605	12-13
TACATGG	14315	0.0	21.207989	2
CAGAGTA	27745	0.0	21.063282	10-11
AGAGTAC	26470	0.0	20.309847	10-11
GTACTTT	21790	0.0	20.252134	14-15
ACATGGG	14515	0.0	19.91207	3
AGTACTT	21070	0.0	19.312515	12-13
>>END_MODULE