FastQCFastQC Report
Wed 25 May 2016
SRR2049345_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049345_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5625619
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53150.045.0265121
GTATCAA90750.035.955611
TCAACGC96950.032.585844
ATCAACG98100.032.2996863
CAACGCA103800.030.5260075
AACGCAG108650.029.243666
TATCAAC111100.028.8587672
ACGCAGA125900.024.9755557
GTACATG145100.024.4643331
CGCAGAG130100.023.916388
TACATGG144400.023.2129212
ACATGGG147750.021.8275033
GTGGTAT33500.021.613731
GCAGAGT146650.020.3519679
CATGGGG108050.018.5784954
AGAGTAC123300.018.1831910-11
CAGAGTA138000.017.69380410-11
GAGTACA111450.017.6761511
TGGTATC34600.016.9840552
GTATAGG20500.016.9719451
Produced by FastQC (version 0.11.5)