##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049345_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5625619
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.87887697336062	31.0	31.0	34.0	28.0	34.0
2	31.074478381845623	31.0	31.0	34.0	28.0	34.0
3	31.129478018330072	31.0	31.0	34.0	28.0	34.0
4	34.74095259561659	37.0	35.0	37.0	32.0	37.0
5	34.76499812731719	37.0	35.0	37.0	32.0	37.0
6	34.74674538037503	37.0	35.0	37.0	32.0	37.0
7	34.70203972220657	37.0	35.0	37.0	32.0	37.0
8	34.66929185926029	37.0	35.0	37.0	32.0	37.0
9	36.261253028333414	39.0	37.0	39.0	32.0	39.0
10-11	36.22491018321717	39.0	37.0	39.0	32.0	39.0
12-13	36.17539021039285	39.0	37.0	39.0	32.0	39.0
14-15	37.39349980864328	40.0	38.0	41.0	32.0	41.0
16-17	37.360757545080816	40.0	38.0	41.0	32.0	41.0
18-19	37.3865693712994	40.0	38.0	41.0	32.0	41.0
20-21	37.45810612129972	40.0	38.0	41.0	32.0	41.0
22-23	37.40470853074124	40.0	38.0	41.0	32.0	41.0
24-25	37.40653588520659	40.0	38.0	41.0	32.0	41.0
26-27	37.38907496223971	40.0	38.0	41.0	32.0	41.0
28-29	37.31850761667293	40.0	38.0	41.0	32.0	41.0
30-31	37.243075473116825	40.0	37.5	41.0	31.5	41.0
32-33	37.181432301049895	40.0	37.0	41.0	31.0	41.0
34-35	37.063738941439155	40.0	37.0	41.0	31.0	41.0
36-37	36.97794029421473	40.0	37.0	41.0	31.0	41.0
38-39	36.871857034754754	40.0	37.0	41.0	30.0	41.0
40-41	36.76554811123896	40.0	37.0	41.0	30.0	41.0
42-43	36.62129029712108	40.0	37.0	41.0	30.0	41.0
44-45	36.50310303630587	40.0	36.5	41.0	30.0	41.0
46-47	36.38763858697149	40.0	36.0	41.0	30.0	41.0
48-49	36.25741309534115	40.0	36.0	41.0	30.0	41.0
50-51	36.11690402425049	39.5	36.0	41.0	29.5	41.0
52-53	35.97216306330023	39.0	35.0	41.0	29.0	41.0
54-55	35.83115786902739	39.0	35.0	41.0	29.0	41.0
56-57	35.699854807088784	39.0	35.0	41.0	28.0	41.0
58-59	35.48836181405104	39.0	35.0	41.0	28.0	41.0
60-61	35.26824372571268	39.0	35.0	40.0	28.0	41.0
62-63	35.05750887146819	38.0	34.5	40.0	28.0	41.0
64-65	34.80543074459895	38.0	34.0	40.0	27.0	41.0
66-67	34.52891219615121	37.0	34.0	40.0	26.5	41.0
68-69	34.221525755654625	37.0	34.0	40.0	26.0	41.0
70-71	33.90804585237642	36.0	34.0	39.0	26.0	41.0
72-73	33.522235064265814	36.0	33.5	39.0	26.0	41.0
74-75	33.116761017765334	35.0	33.0	39.0	25.0	40.0
76-77	32.69802540840395	35.0	33.0	37.5	24.0	40.0
78-79	32.27081704253345	35.0	33.0	37.0	22.5	39.0
80-81	31.79270885568326	35.0	32.5	36.5	19.5	39.0
82-83	31.361536303827187	35.0	32.0	36.0	16.5	38.5
84-85	30.955392819883464	35.0	32.0	36.0	8.5	37.0
86-87	30.503849887452386	35.0	32.0	35.0	2.0	37.0
88-89	30.107643354446864	35.0	31.0	35.0	2.0	36.5
90-91	29.75959943252467	35.0	31.0	35.0	2.0	36.0
92-93	29.393399286371864	35.0	31.0	35.0	2.0	36.0
94-95	29.074578015325958	34.5	31.0	35.0	2.0	36.0
96-97	28.700957085789135	34.0	30.5	35.0	2.0	35.0
98-99	28.383501175603964	34.0	30.0	35.0	2.0	35.0
100	28.210041063925587	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4249454046569028
1101	2	0.4241192913513956
1101	3	0.3877686331069867
1101	4	0.37583600815679574
1101	5	0.41227390354887916
1101	6	0.46356519746912994
1101	7	0.4397696851798827
1101	8	0.4461144911272825
1101	9	0.5919256903948948
1101	10-11	0.5710857414516184
1101	12-13	0.5682368770367177
1101	14-15	0.7148099329083948
1101	16-17	0.663194720629555
1101	18-19	0.6271650496592969
1101	20-21	0.6269651749210681
1101	22-23	0.65542440815112
1101	24-25	0.6775754224416346
1101	26-27	0.6405851007494476
1101	28-29	0.6502839072444786
1101	30-31	0.6222781491149192
1101	32-33	0.6605273403064729
1101	34-35	0.6391250660355325
1101	36-37	0.6293057677094538
1101	38-39	0.682895740832933
1101	40-41	0.7510192230058621
1101	42-43	0.7310588831571039
1101	44-45	0.7130449758195212
1101	46-47	0.7013336496651021
1101	48-49	0.5656293181837668
1101	50-51	0.6440506183273627
1101	52-53	0.6150984662572583
1101	54-55	0.6539075832946608
1101	56-57	0.6374728060095336
1101	58-59	0.7181311562015722
1101	60-61	0.6649799219033738
1101	62-63	0.662531467079269
1101	64-65	0.6298376360891424
1101	66-67	0.6116427583952415
1101	68-69	0.6220462427649807
1101	70-71	0.6073520817216504
1101	72-73	0.6647380473483508
1101	74-75	0.6209277603805248
1101	76-77	0.632631557545217
1101	78-79	0.6476810135565785
1101	80-81	0.6310057410419923
1101	82-83	0.6311216239464237
1101	84-85	0.5324829064373198
1101	86-87	0.5792473299423833
1101	88-89	0.6850036303335187
1101	90-91	0.7311748368607347
1101	92-93	0.7401190420322834
1101	94-95	0.7704418311692294
1101	96-97	0.7930904439881346
1101	98-99	0.8443509669474452
1101	100	0.8266787609984547
1102	1	0.2860834984251177
1102	2	0.31517209158824144
1102	3	0.3023818768601032
1102	4	0.3540668717821873
1102	5	0.26915169434464303
1102	6	0.3974530130514111
1102	7	0.4407486473423887
1102	8	0.41334560959874267
1102	9	0.4639599423796241
1102	10-11	0.4382211118153023
1102	12-13	0.5245270836302467
1102	14-15	0.5974046107467998
1102	16-17	0.5800450502893497
1102	18-19	0.596961946592991
1102	20-21	0.5434828926104913
1102	22-23	0.5240618563933879
1102	24-25	0.5795661823791605
1102	26-27	0.5652919394554345
1102	28-29	0.5926473480196819
1102	30-31	0.6016475270466017
1102	32-33	0.6080165850453341
1102	34-35	0.6404370859387569
1102	36-37	0.5676902034272686
1102	38-39	0.6204677558747349
1102	40-41	0.6965726300791459
1102	42-43	0.6348141787827473
1102	44-45	0.5742892302181346
1102	46-47	0.5238969721849998
1102	48-49	0.5517923540649079
1102	50-51	0.6008573550108096
1102	52-53	0.5899298515180718
1102	54-55	0.5511392400548445
1102	56-57	0.4975768134850185
1102	58-59	0.6086051867141293
1102	60-61	0.5829995744884613
1102	62-63	0.6039394453593232
1102	64-65	0.5592515658239634
1102	66-67	0.6359102357471613
1102	68-69	0.6701332991919386
1102	70-71	0.6857599245884458
1102	72-73	0.6169648889476349
1102	74-75	0.5533231452275302
1102	76-77	0.5915783031272497
1102	78-79	0.5706105238063515
1102	80-81	0.5438119579403953
1102	82-83	0.5170941308495607
1102	84-85	0.527193799130302
1102	86-87	0.5399940289288025
1102	88-89	0.48591407843365886
1102	90-91	0.4981688896113461
1102	92-93	0.49232164381721333
1102	94-95	0.48646322141762255
1102	96-97	0.4955468825521514
1102	98-99	0.5331553267497213
1102	100	0.48255074248132246
1103	1	0.30359400654434054
1103	2	0.326183189371708
1103	3	0.3581477851950332
1103	4	0.34441815039601664
1103	5	0.3470341445255727
1103	6	0.3596568453650093
1103	7	0.41630892917165596
1103	8	0.40073626972030496
1103	9	0.48765295410235154
1103	10-11	0.44848711590766754
1103	12-13	0.45802807648372124
1103	14-15	0.5079397436480448
1103	16-17	0.427599203642373
1103	18-19	0.5079341366160648
1103	20-21	0.4801380347850994
1103	22-23	0.43690323167184886
1103	24-25	0.51047990996436
1103	26-27	0.5097814514956696
1103	28-29	0.5223726753589375
1103	30-31	0.5147987532458345
1103	32-33	0.5323849387229203
1103	34-35	0.5634448775705749
1103	36-37	0.5355675129455619
1103	38-39	0.5014797608339592
1103	40-41	0.5135051664417674
1103	42-43	0.5460067117182632
1103	44-45	0.4619548653923644
1103	46-47	0.49124146714009953
1103	48-49	0.5638358178635627
1103	50-51	0.4542323512309139
1103	52-53	0.4777247088394816
1103	54-55	0.526100382693123
1103	56-57	0.4531866875891879
1103	58-59	0.516278280889054
1103	60-61	0.49815707028017187
1103	62-63	0.5141774278004334
1103	64-65	0.5159308993521776
1103	66-67	0.5469999554298539
1103	68-69	0.564916635381195
1103	70-71	0.4585000993709727
1103	72-73	0.4684842067514694
1103	74-75	0.46993272701950417
1103	76-77	0.5715091561748622
1103	78-79	0.43531836281290026
1103	80-81	0.42606538252889337
1103	82-83	0.4242702943024703
1103	84-85	0.41181958912919114
1103	86-87	0.35359501271051386
1103	88-89	0.28582295766840105
1103	90-91	0.25813905360856637
1103	92-93	0.2627896457122034
1103	94-95	0.3283634522434582
1103	96-97	0.22801208228201375
1103	98-99	0.16693008508818252
1103	100	0.12435622383885914
1104	1	0.10661108954278475
1104	2	0.16890969118488286
1104	3	0.1652585747102968
1104	4	0.20364477853224372
1104	5	0.2531682545739429
1104	6	0.261106331791197
1104	7	0.27668481467335937
1104	8	0.30736581038526367
1104	9	0.3131745919818627
1104	10-11	0.3365553807326904
1104	12-13	0.3072406297130499
1104	14-15	0.4098311165086628
1104	16-17	0.37890265909307885
1104	18-19	0.3952237018739524
1104	20-21	0.3582318390019239
1104	22-23	0.3707093338004057
1104	24-25	0.36020943360814783
1104	26-27	0.2719701071512475
1104	28-29	0.32623367267753167
1104	30-31	0.27795917283682314
1104	32-33	0.2963750069068354
1104	34-35	0.2944560497187396
1104	36-37	0.2938389479830761
1104	38-39	0.37375303385280745
1104	40-41	0.3711506268644911
1104	42-43	0.3954139703706332
1104	44-45	0.32987790832006425
1104	46-47	0.2810440749669354
1104	48-49	0.2730061158409427
1104	50-51	0.3036971383937441
1104	52-53	0.3169526116554593
1104	54-55	0.28171163742832306
1104	56-57	0.28363875619596257
1104	58-59	0.35056567766480384
1104	60-61	0.28946948665316796
1104	62-63	0.28468386493315023
1104	64-65	0.3559342196257589
1104	66-67	0.3857375950435369
1104	68-69	0.36353653122383633
1104	70-71	0.2905524558874575
1104	72-73	0.34102072021994445
1104	74-75	0.3677662065212033
1104	76-77	0.40051756219165213
1104	78-79	0.3324669652830252
1104	80-81	0.3259426468982305
1104	82-83	0.2527925893671714
1104	84-85	0.24336010448145018
1104	86-87	0.2134076424206306
1104	88-89	0.17684844897348384
1104	90-91	0.19121341323465302
1104	92-93	0.15737992013472635
1104	94-95	0.18873236898702572
1104	96-97	0.21833022555448878
1104	98-99	0.22568592896051953
1104	100	0.1872031867044619
1105	1	0.13107884073207998
1105	2	0.11018845568020197
1105	3	0.1312348601746578
1105	4	0.19042166748447897
1105	5	0.172114752239672
1105	6	0.21509287295718593
1105	7	0.17495968004953255
1105	8	0.20489083512864426
1105	9	0.2824249121285263
1105	10-11	0.290641677586315
1105	12-13	0.25813646099137344
1105	14-15	0.23251236891402272
1105	16-17	0.3170313411780583
1105	18-19	0.26865262221671316
1105	20-21	0.24455047028494192
1105	22-23	0.2653963476145478
1105	24-25	0.26147474622207767
1105	26-27	0.2595015949391737
1105	28-29	0.3563906412286286
1105	30-31	0.3361002624609384
1105	32-33	0.3581074244056097
1105	34-35	0.3815744429322834
1105	36-37	0.3547351602073263
1105	38-39	0.3819576820593866
1105	40-41	0.38384704758059485
1105	42-43	0.38269882102027
1105	44-45	0.35739991881070665
1105	46-47	0.3382102254736026
1105	48-49	0.37454331955589737
1105	50-51	0.3845154899060148
1105	52-53	0.3941971399756099
1105	54-55	0.43717048389799373
1105	56-57	0.39446432685078037
1105	58-59	0.409129602780979
1105	60-61	0.40589966266901456
1105	62-63	0.3485754853829732
1105	64-65	0.40733969281926363
1105	66-67	0.45148877478092686
1105	68-69	0.4132000314042372
1105	70-71	0.377862734315471
1105	72-73	0.3619922134425053
1105	74-75	0.3615656742687676
1105	76-77	0.4590545603696299
1105	78-79	0.39184095814818676
1105	80-81	0.4235691075470207
1105	82-83	0.3669512630496179
1105	84-85	0.37094818663888773
1105	86-87	0.35078507162081607
1105	88-89	0.31319675927552026
1105	90-91	0.3440999404519367
1105	92-93	0.3455779546565374
1105	94-95	0.30722302791773615
1105	96-97	0.2594393749630761
1105	98-99	0.258855998719973
1105	100	0.21129029614617068
1106	1	0.1100279552701302
1106	2	0.07848914666084639
1106	3	0.15444334413567162
1106	4	0.14368151739067514
1106	5	0.1526506054406056
1106	6	0.20981956952058312
1106	7	0.17932464138207393
1106	8	0.22937088363456581
1106	9	0.24131728388526597
1106	10-11	0.19653674920120778
1106	12-13	0.22682382657046674
1106	14-15	0.32472568058746987
1106	16-17	0.3272870840966533
1106	18-19	0.2850737085664079
1106	20-21	0.22012075811727527
1106	22-23	0.2817753916817267
1106	24-25	0.29312114488587326
1106	26-27	0.257840781778512
1106	28-29	0.2972974961226953
1106	30-31	0.32569150851411877
1106	32-33	0.3637056260255207
1106	34-35	0.3580416327917959
1106	36-37	0.23255542515950367
1106	38-39	0.3171347744494426
1106	40-41	0.35244232054082403
1106	42-43	0.36122665472731796
1106	44-45	0.31564592622387266
1106	46-47	0.2962122732473631
1106	48-49	0.2611624336363434
1106	50-51	0.2908738846344292
1106	52-53	0.31484218871032965
1106	54-55	0.36392024365637354
1106	56-57	0.2684046897300192
1106	58-59	0.37265377566341584
1106	60-61	0.3189384902899306
1106	62-63	0.32543649988898693
1106	64-65	0.30238642553330664
1106	66-67	0.34419629777548266
1106	68-69	0.34394809803124105
1106	70-71	0.30647608259717174
1106	72-73	0.30485303346722503
1106	74-75	0.31483753625568767
1106	76-77	0.35902510217167105
1106	78-79	0.19736937529914655
1106	80-81	0.24151201012333345
1106	82-83	0.2383913971273195
1106	84-85	0.27918704400400784
1106	86-87	0.281564026455392
1106	88-89	0.2460423639979865
1106	90-91	0.25341445104544746
1106	92-93	0.23130216872325704
1106	94-95	0.26739440026936023
1106	96-97	0.27508968613316753
1106	98-99	0.2633290687790044
1106	100	0.29789797014974084
1107	1	0.1729353789742234
1107	2	0.11803592275311914
1107	3	0.11268317024055108
1107	4	0.13131632576331498
1107	5	0.13027472511998184
1107	6	0.2084791252439615
1107	7	0.21140127478631143
1107	8	0.3651167570244027
1107	9	0.3056297127062919
1107	10-11	0.2830431277740999
1107	12-13	0.24462374456008718
1107	14-15	0.3376094560411431
1107	16-17	0.35832184498215014
1107	18-19	0.3285231404745872
1107	20-21	0.28777478576592586
1107	22-23	0.3707769493668991
1107	24-25	0.32574485685557875
1107	26-27	0.3300370478769139
1107	28-29	0.4124869310550707
1107	30-31	0.3685901238372864
1107	32-33	0.3665058724706398
1107	34-35	0.3373138990780191
1107	36-37	0.2853727085308506
1107	38-39	0.31721792325579656
1107	40-41	0.34650464330831454
1107	42-43	0.34535519395775793
1107	44-45	0.27786695421657015
1107	46-47	0.286896903850959
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2216	1	-1.269153055732776
2216	2	-1.1893528968949951
2216	3	-1.2022915183082468
2216	4	-1.4156810359368777
2216	5	-1.3584862629237833
2216	6	-2.6395493544578557
2216	7	-2.602241352502176
2216	8	-3.4832633988418102
2216	9	-2.9722438810205247
2216	10-11	-2.1674924265081614
2216	12-13	-1.9652003799733109
2216	14-15	-2.182410029713573
2216	16-17	-2.5923461671408603
2216	18-19	-2.2519724030595896
2216	20-21	-1.97462024930811
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2216	24-25	-2.2962406508233784
2216	26-27	-2.402895905654759
2216	28-29	-2.8261174993455853
2216	30-31	-2.3938928183339456
2216	32-33	-2.542738488035276
2216	34-35	-3.4609868853984693
2216	36-37	-2.5211412443977395
2216	38-39	-2.3838262888423856
2216	40-41	-2.919956202506306
2216	42-43	-2.7183816677084707
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2216	50-51	-2.8250643878781503
2216	52-53	-3.151399240868365
2216	54-55	-2.5959934148724884
2216	56-57	-2.5398354681305264
2216	58-59	-3.558456512383014
2216	60-61	-4.320024057943407
2216	62-63	-3.4136106273459283
2216	64-65	-3.3569139951341924
2216	66-67	-3.038010302056758
2216	68-69	-3.35091187066255
2216	70-71	-3.1253290205421536
2216	72-73	-3.6720970991847217
2216	74-75	-4.034451802827153
2216	76-77	-4.636676463225477
2216	78-79	-3.5944909411127455
2216	80-81	-3.4105500563506475
2216	82-83	-3.6509908984160795
2216	84-85	-3.6928005259906627
2216	86-87	-3.2568416373552402
2216	88-89	-3.1143963136389736
2216	90-91	-3.2790847930246763
2216	92-93	-3.2883471056226554
2216	94-95	-3.170720437973227
2216	96-97	-3.364310868469474
2216	98-99	-3.0644601195037815
2216	100	-2.6223177679919054
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	102037.0
3	12943.0
4	8415.0
5	7128.0
6	7067.0
7	7866.0
8	9089.0
9	10464.0
10	11610.0
11	13019.0
12	14597.0
13	16039.0
14	17787.0
15	18127.0
16	17805.0
17	18682.0
18	17969.0
19	16838.0
20	17135.0
21	18078.0
22	20131.0
23	23927.0
24	29405.0
25	37033.0
26	46631.0
27	57499.0
28	73650.0
29	93843.0
30	115848.0
31	138216.0
32	168038.0
33	213955.0
34	284242.0
35	397619.0
36	636534.0
37	1076669.0
38	1364776.0
39	483645.0
40	1263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.218701585566585	20.290583496040796	13.993209133995684	23.49750578439694
2	15.771905112566662	20.25387227145814	40.105548638994456	23.868673976980745
3	18.57711361744066	25.005859575756045	30.18161661693882	26.23541018986448
4	12.99876937084974	16.720231045287864	38.348929601610486	31.93206998225191
5	13.386734892045645	37.42990311815971	35.57693744117837	13.606424548616273
6	31.260614730498325	37.58623829423082	17.55451903422358	13.598627941047276
7	27.157894811719707	32.37318813517968	22.432944018472003	18.035973034628608
8	24.981398624280665	36.22431196249849	20.539190929072916	18.25509848414793
9	24.848449928368492	16.9489424572096	21.577692796872398	36.6249148175495
10-11	23.986920258927302	26.85389991463935	28.89568217385119	20.26349765258216
12-13	24.335425437178866	24.458951001911302	28.831259828271723	22.37436373263811
14-15	21.802248709592806	26.712313663422684	25.93405030031699	25.551387326667523
16-17	21.45162798985247	29.97328324772141	26.676305491409348	21.898783271016768
18-19	21.344129337224437	29.031636222695635	28.54077270391598	21.08346173616395
20-21	22.386325442934652	28.264041157400737	28.11877921999399	21.23085417967062
22-23	22.120004360483986	28.118337346745083	28.103203693109425	21.65845459966151
24-25	21.954453372180367	28.210899622720966	28.043821700088774	21.7908253050099
26-27	21.687287540624688	28.1737491793262	28.151200655543203	21.98776262450591
28-29	21.688806803091627	28.221740839524667	28.317501675370053	21.771950682013653
30-31	21.974355479338108	28.490431340769955	27.858187538127616	21.677025641764313
32-33	21.70028070231595	28.253386077271823	28.203105063755967	21.843228156656256
34-35	21.921813249189814	27.90320389343971	28.240979491440065	21.93400336593041
36-37	22.022001436105572	28.11041510848501	27.88593025534441	21.98165320006501
38-39	21.83222329588476	28.107645477025656	28.153790836865216	21.90634039022437
40-41	21.68269639855843	27.776893090575168	28.445298723303008	22.095111787563397
42-43	21.46878936627718	27.809280678755528	28.527645218217657	22.194284736749637
44-45	21.516762737707698	27.838397575757362	28.462827482277813	22.18201220425713
46-47	21.439733565829595	28.005355285570477	28.37369565383616	22.181215494763766
48-49	21.33779821469947	27.89720127228314	28.365656392863563	22.39934412015383
50-51	21.279225281421063	27.804959284347703	28.35696298737106	22.558852446860175
52-53	21.218334057824052	27.789288521070677	28.447749667221807	22.544627753883464
54-55	21.314558765984955	27.984231255477543	28.24909160697032	22.45211837156718
56-57	21.32141778785746	27.774087004950893	28.190108033346412	22.71438717384524
58-59	21.308842759107925	27.386947652637904	28.30681566906197	22.997393919192202
60-61	21.443293176111514	27.553386800227504	28.05171487433016	22.951605149330824
62-63	21.40997949800657	27.531041284145502	28.01710897086466	23.04187024698327
64-65	21.462759270395868	27.364449277903297	28.05290302114492	23.119888430555914
66-67	21.405775085446276	27.466979532546937	27.846186564774243	23.28105881723254
68-69	21.69585363262254	27.544920721078764	27.774780025277146	22.98444562102155
70-71	21.657063187358137	27.38052761684549	27.86788711333611	23.09452208246026
72-73	21.61908532474429	27.372829419771183	27.96221315098069	23.045872104503832
74-75	21.822144733121966	27.325805115110434	27.874462323048938	22.97758782871866
76-77	21.960153404546922	27.328885781005987	27.736109326745726	22.974851487701365
78-79	22.07171559154627	27.37032606361768	27.879263950736817	22.67869439409923
80-81	22.0991905098154	27.188746907487165	27.990670849231186	22.72139173346625
82-83	22.047534482780083	27.230251563503277	27.993505755680065	22.728708198036575
84-85	22.240173168394456	27.016378482407983	27.919776074616188	22.823672274581373
86-87	22.51183481510802	27.01655967232739	27.824165108073707	22.647440404490883
88-89	22.588993587139534	26.81598503739983	27.882708678028916	22.712312697431717
90-91	22.802352834692442	26.633262201549023	27.83080480749029	22.73358015626824
92-93	23.060464548911995	26.68278241411514	27.592265010245935	22.664488026726925
94-95	23.126194739677892	26.376251198073835	27.620167472495428	22.877386589752845
96-97	23.25814279281978	26.25741273982472	27.610837136322242	22.87360733103326
98-99	23.363153459201556	26.25228441527946	27.52807291073213	22.85648921478685
100	23.50386686336206	26.146136096312244	27.554745531114	22.7952515092117
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1486.0
1	1125.5
2	1012.0
3	1255.5
4	1190.0
5	1042.0
6	892.5
7	792.5
8	796.0
9	806.0
10	712.5
11	647.0
12	685.5
13	775.0
14	892.0
15	1020.0
16	1211.5
17	1530.5
18	2062.5
19	2885.5
20	4174.0
21	5941.0
22	8371.5
23	11898.0
24	16922.0
25	23536.5
26	32105.5
27	42976.0
28	56547.5
29	72132.5
30	87212.0
31	100480.5
32	112868.5
33	125145.0
34	137915.0
35	149844.0
36	161103.5
37	172693.5
38	183164.0
39	190700.0
40	196794.0
41	202401.5
42	205519.0
43	207571.0
44	207149.0
45	204613.0
46	202021.5
47	196786.5
48	190882.5
49	187291.0
50	183557.5
51	177863.0
52	169594.5
53	161659.5
54	154645.0
55	146096.5
56	134103.5
57	119862.5
58	106622.5
59	95181.5
60	83277.5
61	70365.5
62	59786.5
63	50351.5
64	41193.0
65	33476.0
66	26942.5
67	21166.0
68	16511.5
69	13040.0
70	10071.0
71	7686.0
72	5930.5
73	4504.5
74	3428.0
75	2582.0
76	1872.5
77	1353.0
78	998.0
79	681.0
80	442.0
81	302.5
82	259.5
83	211.0
84	163.0
85	127.0
86	93.0
87	69.0
88	57.5
89	53.0
90	44.5
91	35.5
92	37.5
93	37.5
94	34.0
95	38.0
96	34.5
97	33.5
98	47.0
99	59.0
100	116.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01786470075559685
2	0.03981784049008651
3	0.04168430176305932
4	0.044066261863805564
5	0.047763632766456454
6	0.045097259519352444
7	0.04278640270519564
8	0.04228867969906956
9	0.04257309284542732
10-11	0.04299971256496396
12-13	0.044288459634397565
14-15	0.04255531702377996
16-17	0.038769067012892275
18-19	0.042048706106830196
20-21	0.04139988861670156
22-23	0.042484213737190524
24-25	0.039017928515955314
26-27	0.03894682522936587
28-29	0.03972007347102603
30-31	0.03972007347102603
32-33	0.03953342734372875
34-35	0.03930234166231308
36-37	0.03692038156156682
38-39	0.03706258813474571
40-41	0.03844021431241611
42-43	0.038235792363471466
44-45	0.03714257933215882
46-47	0.03583605644107787
48-49	0.03604936630084618
50-51	0.037435880389340266
52-53	0.03783583637640587
54-55	0.03714257933215882
56-57	0.0352850059700097
58-59	0.03627156407143818
60-61	0.03580939270860682
62-63	0.03589827181684362
64-65	0.03300970079914761
66-67	0.0339518193464577
68-69	0.03486727416129674
70-71	0.036431546466264425
72-73	0.03312524363985545
74-75	0.03357852709186313
76-77	0.03424512040363914
78-79	0.03460063683658634
80-81	0.034769507142236256
82-83	0.03324078648056329
84-85	0.0348406104288257
86-87	0.03478728296388362
88-89	0.0355072037406017
90-91	0.03589827181684362
92-93	0.03425400831446282
94-95	0.03571162568954635
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5625619.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.45066395487508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.36765368166171	50.50847312368808
2	13.018139769628323	16.7805550321982
3	3.943277147999632	7.624404910399875
4	1.67540732728914	4.319244585545912
5	0.8590327580208479	2.7682615806715596
6	0.5153797564648953	1.9929940495838596
7	0.31641644736489294	1.4275275083237096
8	0.2370758003421995	1.2223754191750529
9	0.16488773609548513	0.9564411662433214
>10	0.8524698555459947	9.536476740487123
>50	0.03941644838894333	1.6973136573292178
>100	0.010705233101123932	1.0674827673153136
>500	1.1043091965164409E-4	0.055354791382718
>1k	2.7607177101948492E-5	0.0430946676560806
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.5551643294720104E-5	0.0
5	0.0	0.0	0.0	5.332746494208015E-5	0.0
6	0.0	0.0	0.0	5.332746494208015E-5	0.0
7	0.0	0.0	0.0	5.332746494208015E-5	0.0
8	0.0	0.0	0.0	5.332746494208015E-5	0.0
9	0.0	0.0	0.0	1.2443075153152036E-4	0.0
10-11	0.0	0.0	0.0	2.133098597683206E-4	0.0
12-13	0.0	8.887910823680026E-6	0.0	2.666373247104008E-4	0.0
14-15	0.0	1.7775821647360052E-5	0.0	3.110768788288009E-4	0.0
16-17	0.0	1.7775821647360052E-5	0.0	3.4662852212352093E-4	0.0
18-19	0.0	1.7775821647360052E-5	0.0	4.6217136283136135E-4	0.0
20-21	0.0	1.7775821647360052E-5	0.0	7.999119741312023E-4	0.0
22-23	0.0	2.6663732471040078E-5	0.0	0.002399735922393607	0.0
24-25	0.0	5.332746494208015E-5	0.0	0.005350522315855375	0.0
26-27	0.0	7.110328658944021E-5	0.0	0.011225431370307872	0.0
28-29	0.0	8.887910823680025E-5	0.0	0.03684039036415371	0.0
30-31	0.0	8.887910823680025E-5	0.0	0.11208544339742879	0.0
32-33	0.0	8.887910823680025E-5	0.0	0.2771694990364616	0.0
34-35	0.0	8.887910823680025E-5	0.0	0.5381718882846492	0.0
36-37	0.0	9.776701906048027E-5	0.0	0.9350437702944334	0.0
38-39	0.0	1.2443075153152036E-4	0.0	1.4326601214906307	0.0
40-41	0.0	1.2443075153152036E-4	0.0	1.9164024438910634	0.0
42-43	0.0	1.2443075153152036E-4	0.0	2.379951432900095	0.0
44-45	0.0	1.333186623552004E-4	0.0	2.937214553634009	0.0
46-47	0.0	1.4220657317888042E-4	0.0	3.56883393631883	0.0
48-49	0.0	1.4220657317888042E-4	0.0	4.187636951595905	0.0
50-51	0.0	1.5998239482624044E-4	0.0	4.769350715005762	0.0
52-53	0.0	1.5998239482624044E-4	0.0	5.31090000940341	0.0
54-55	0.0	1.5998239482624044E-4	0.0	5.890871742291826	0.0
56-57	0.0	1.777582164736005E-4	0.0	6.544239842762192	0.0
58-59	0.0	1.777582164736005E-4	0.0	7.218556749043972	0.0
60-61	0.0	1.777582164736005E-4	0.0	7.871889297871043	0.0
62-63	0.0	1.777582164736005E-4	0.0	8.479813510299934	0.0
64-65	0.0	1.777582164736005E-4	0.0	9.103682634746505	0.0
66-67	0.0	1.9553403812096055E-4	0.0	9.776577475296497	0.0
68-69	0.0	2.133098597683206E-4	0.0	10.484321814186137	0.0
70-71	0.0	2.133098597683206E-4	0.0	11.189239797433848	0.0
72-73	0.0	2.133098597683206E-4	0.0	11.869760465470556	0.0
74-75	0.0	2.133098597683206E-4	0.0	12.527510305976996	0.0
76-77	8.887910823680026E-6	2.2219777059200062E-4	0.0	13.210724010993278	0.0
78-79	1.7775821647360052E-5	2.3108568141568065E-4	0.0	13.936466369300872	0.0
80-81	1.7775821647360052E-5	2.3108568141568065E-4	0.0	14.673149745832415	0.0
82-83	1.7775821647360052E-5	2.3108568141568065E-4	0.0	15.392350601773778	0.0
84-85	2.6663732471040078E-5	2.3108568141568065E-4	0.0	16.0768175022162	0.0
86-87	3.5551643294720104E-5	2.3108568141568065E-4	0.0	16.769221307024168	0.0
88	3.5551643294720104E-5	2.3108568141568065E-4	0.0	17.303855806800993	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5315	0.0	45.026512	1
GTATCAA	9075	0.0	35.95561	1
TCAACGC	9695	0.0	32.58584	4
ATCAACG	9810	0.0	32.299686	3
CAACGCA	10380	0.0	30.526007	5
AACGCAG	10865	0.0	29.24366	6
TATCAAC	11110	0.0	28.858767	2
ACGCAGA	12590	0.0	24.975555	7
GTACATG	14510	0.0	24.464333	1
CGCAGAG	13010	0.0	23.91638	8
TACATGG	14440	0.0	23.212921	2
ACATGGG	14775	0.0	21.827503	3
GTGGTAT	3350	0.0	21.61373	1
GCAGAGT	14665	0.0	20.351967	9
CATGGGG	10805	0.0	18.578495	4
AGAGTAC	12330	0.0	18.18319	10-11
CAGAGTA	13800	0.0	17.693804	10-11
GAGTACA	11145	0.0	17.676151	1
TGGTATC	3460	0.0	16.984055	2
GTATAGG	2050	0.0	16.971945	1
>>END_MODULE