FastQCFastQC Report
Wed 25 May 2016
SRR2049345_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049345_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5625619
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT165240.29372767690097745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109290.19427195478399797No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106760.18977467190721592No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT79130.14066007669556008TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59640.10601500030485533No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87200.056.930261
GTATCAA148550.037.1527671
GTGGTAT35900.031.4276921
ATCAACG182150.029.7487343
TCAACGC182750.029.5739124
CAACGCA189350.028.5430835
TATCAAC192850.028.2443852
AACGCAG197900.027.3778516
TGGTATC42300.023.9983672
GTACATG144450.023.790061
ACGCAGA227750.023.6243767
CGCAGAG231900.023.0190728
TACATGG144350.022.6600462
ACATGGG149100.021.339263
GCAGAGT248950.021.064529
GAGTACT171900.020.70363412-13
CAGAGTA244000.019.94983710-11
AGAGTAC231800.019.1636110-11
GTACTTT187750.018.71111114-15
CATGGGG109600.018.6100484
Produced by FastQC (version 0.11.5)