##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049345_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5625619
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.724859611004586	31.0	31.0	34.0	30.0	34.0
2	31.84317352454903	31.0	31.0	34.0	30.0	34.0
3	31.88160200681916	33.0	31.0	34.0	30.0	34.0
4	35.647709345407144	37.0	35.0	37.0	33.0	37.0
5	35.71172825603725	37.0	35.0	37.0	33.0	37.0
6	35.79275116213878	37.0	35.0	37.0	35.0	37.0
7	35.739272069438044	37.0	35.0	37.0	33.0	37.0
8	35.74929354440818	37.0	35.0	37.0	33.0	37.0
9	37.48361646958317	39.0	37.0	39.0	34.0	39.0
10-11	37.4414335560229	39.0	37.0	39.0	34.0	39.0
12-13	37.38592233139144	39.0	37.0	39.0	34.0	39.0
14-15	38.664181754932216	40.0	38.0	41.0	34.0	41.0
16-17	38.68519526473442	40.0	38.0	41.0	34.0	41.0
18-19	38.74708498744761	40.0	38.0	41.0	34.0	41.0
20-21	38.77701929334354	40.0	38.0	41.0	34.0	41.0
22-23	38.74357612913352	40.0	38.0	41.0	34.0	41.0
24-25	38.717086066439975	40.0	38.0	41.0	34.0	41.0
26-27	38.59790984778742	40.0	38.0	41.0	34.0	41.0
28-29	38.50722320512641	40.0	38.0	41.0	34.0	41.0
30-31	38.405783790192686	40.0	38.0	41.0	34.0	41.0
32-33	38.27427763949176	40.0	38.0	41.0	34.0	41.0
34-35	38.16670085549697	40.0	38.0	41.0	34.0	41.0
36-37	38.044861196607876	40.0	38.0	41.0	33.0	41.0
38-39	37.9362314618178	40.0	37.0	41.0	33.0	41.0
40-41	37.83999058592485	40.0	37.0	41.0	33.0	41.0
42-43	37.710480304478494	40.0	37.0	41.0	33.0	41.0
44-45	37.54932772731321	40.0	37.0	41.0	33.0	41.0
46-47	37.33732741232565	40.0	37.0	41.0	32.0	41.0
48-49	37.14133111396275	40.0	37.0	41.0	31.5	41.0
50-51	36.9879221291026	40.0	36.0	41.0	31.0	41.0
52-53	36.85043743630701	40.0	36.0	41.0	31.0	41.0
54-55	36.692974230924634	39.0	36.0	41.0	31.0	41.0
56-57	36.5415682256477	39.0	35.0	41.0	31.0	41.0
58-59	36.33717054069961	39.0	35.0	41.0	30.5	41.0
60-61	36.12736838381696	39.0	35.0	41.0	30.0	41.0
62-63	35.904404741949286	38.5	35.0	40.5	30.0	41.0
64-65	35.64513176238917	38.0	35.0	40.0	29.5	41.0
66-67	35.32559848080718	37.5	35.0	40.0	29.0	41.0
68-69	34.976469522731634	37.0	34.0	40.0	29.0	41.0
70-71	34.60341262001568	36.5	34.0	39.0	28.0	41.0
72-73	34.28979984247067	36.0	34.0	39.0	28.0	41.0
74-75	33.972995416148876	35.5	34.0	39.0	28.0	40.5
76-77	33.6390333223775	35.0	34.0	37.5	28.0	40.0
78-79	33.29644737761302	35.0	33.5	37.0	27.0	39.0
80-81	32.97025127368207	35.0	33.0	37.0	26.5	39.0
82-83	32.66925337460642	35.0	33.0	36.0	26.0	38.5
84-85	32.40515345955707	35.0	33.0	36.0	26.0	37.0
86-87	32.17543278348569	35.0	33.0	35.5	26.0	37.0
88-89	31.947544794626154	35.0	33.0	35.0	26.0	36.5
90-91	31.748177844962484	35.0	33.0	35.0	25.0	36.0
92-93	31.559015372352803	35.0	33.0	35.0	25.0	36.0
94-95	31.402273154296445	35.0	33.0	35.0	25.0	36.0
96-97	31.196290310452948	35.0	33.0	35.0	24.0	35.5
98-99	31.03457530629074	35.0	33.0	35.0	23.5	35.0
100	30.90797492684805	35.0	32.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1422991719638489
1101	2	0.21181342480749876
1101	3	0.35201391654500114
1101	4	0.19234522702823398
1101	5	0.1897719785871459
1101	6	0.15465438160332212
1101	7	0.10282756640274471
1101	8	0.14864834080893985
1101	9	0.26936509927506336
1101	10-11	0.2822393526587845
1101	12-13	0.2107942598804442
1101	14-15	0.3424392078666614
1101	16-17	0.2144669048379555
1101	18-19	0.28406880424979875
1101	20-21	0.29666487810256825
1101	22-23	0.28330553248162005
1101	24-25	0.26663822581472374
1101	26-27	0.4040827097404218
1101	28-29	0.3689721673710906
1101	30-31	0.3544268474415162
1101	32-33	0.386320844540073
1101	34-35	0.38853083096633867
1101	36-37	0.38507211234855987
1101	38-39	0.4024169356330134
1101	40-41	0.37956684025742504
1101	42-43	0.3893567019756574
1101	44-45	0.3547490873171526
1101	46-47	0.41504693406604076
1101	48-49	0.43111941189553704
1101	50-51	0.3859678323098237
1101	52-53	0.41870832125981394
1101	54-55	0.39889566713463864
1101	56-57	0.3824497745133044
1101	58-59	0.3841242459834433
1101	60-61	0.42752222338799584
1101	62-63	0.415600127284101
1101	64-65	0.3372563029929836
1101	66-67	0.3537206303899296
1101	68-69	0.4255175023770761
1101	70-71	0.35952633725069205
1101	72-73	0.3778108930231241
1101	74-75	0.35166791169077527
1101	76-77	0.2883023609923754
1101	78-79	0.3320799145392854
1101	80-81	0.36834485299572606
1101	82-83	0.3388051651885746
1101	84-85	0.37208096653638023
1101	86-87	0.3254659630892647
1101	88-89	0.3951250944054365
1101	90-91	0.42020368638545946
1101	92-93	0.45558869110588773
1101	94-95	0.46208544542378505
1101	96-97	0.5182347924545674
1101	98-99	0.5500169641977131
1101	100	0.5712723821152252
1102	1	0.21424662489091872
1102	2	0.1918814510457061
1102	3	0.3537450194565501
1102	4	0.23626258961532187
1102	5	0.1959912151759795
1102	6	0.1396422659015215
1102	7	0.11625827392440158
1102	8	0.1999753823320276
1102	9	0.17220443775185856
1102	10-11	0.23665327818822135
1102	12-13	0.2279200503868779
1102	14-15	0.29033792211356513
1102	16-17	0.2621972945169446
1102	18-19	0.29480567105613886
1102	20-21	0.2468403097638756
1102	22-23	0.2940464448641933
1102	24-25	0.32525519385298907
1102	26-27	0.37099479962117243
1102	28-29	0.36735137159531206
1102	30-31	0.30467341732275344
1102	32-33	0.33568932252028105
1102	34-35	0.3817498816773437
1102	36-37	0.3697034392386982
1102	38-39	0.3726555825770035
1102	40-41	0.3082456526242652
1102	42-43	0.40288751437951476
1102	44-45	0.40354431303255467
1102	46-47	0.3342473061581259
1102	48-49	0.274853714260729
1102	50-51	0.3734004558029298
1102	52-53	0.4392973352914922
1102	54-55	0.35020142102061413
1102	56-57	0.4411008902428648
1102	58-59	0.38289922699615175
1102	60-61	0.4248903699842117
1102	62-63	0.4277109340190961
1102	64-65	0.41769265329447336
1102	66-67	0.4088202684748623
1102	68-69	0.43014213430112846
1102	70-71	0.3762435413790257
1102	72-73	0.40520237107146073
1102	74-75	0.3915715075435955
1102	76-77	0.3380604338514388
1102	78-79	0.36272757681020096
1102	80-81	0.39260249816756954
1102	82-83	0.431926491262864
1102	84-85	0.416075085732011
1102	86-87	0.43482779656795856
1102	88-89	0.41797317166331993
1102	90-91	0.41781432364362203
1102	92-93	0.47527551640512655
1102	94-95	0.4705312292352595
1102	96-97	0.472151406483885
1102	98-99	0.5271707625396154
1102	100	0.5752441286855259
1103	1	0.11505199537142019
1103	2	0.13113252705695544
1103	3	0.3125729483742923
1103	4	0.1450817491673746
1103	5	0.16794859807470885
1103	6	0.13289489419129552
1103	7	0.1465877803199902
1103	8	0.15381787646058598
1103	9	0.20007731218865388
1103	10-11	0.17168598691424108
1103	12-13	0.21513713754631425
1103	14-15	0.25637346324390364
1103	16-17	0.2373564030655615
1103	18-19	0.23803360400577134
1103	20-21	0.22791077716098584
1103	22-23	0.22928981996121678
1103	24-25	0.24348519314740003
1103	26-27	0.2973071301557013
1103	28-29	0.30400611058068705
1103	30-31	0.3151587563544851
1103	32-33	0.32097694763493934
1103	34-35	0.3008386740248099
1103	36-37	0.3031346803559316
1103	38-39	0.2859154682728615
1103	40-41	0.33490613705612304
1103	42-43	0.3645835298619815
1103	44-45	0.3735772737518417
1103	46-47	0.3042516404423097
1103	48-49	0.27913598703840137
1103	50-51	0.32234601517757966
1103	52-53	0.3721205670547363
1103	54-55	0.29998904109541513
1103	56-57	0.2789524964479426
1103	58-59	0.34570421444688293
1103	60-61	0.3216402616271026
1103	62-63	0.209947424660065
1103	64-65	0.2677830684785718
1103	66-67	0.21603211417434665
1103	68-69	0.25330656494755033
1103	70-71	0.2569890382593485
1103	72-73	0.2866429661787535
1103	74-75	0.24221288080610748
1103	76-77	0.2321573403820949
1103	78-79	0.2463395474983443
1103	80-81	0.25896180024701465
1103	82-83	0.23790800503681453
1103	84-85	0.2652343353968618
1103	86-87	0.2636519959853487
1103	88-89	0.25938191482170225
1103	90-91	0.28443791626122206
1103	92-93	0.3659854318361866
1103	94-95	0.27103259503876487
1103	96-97	0.270692667861379
1103	98-99	0.34228834259787533
1103	100	0.39276068464315017
1104	1	0.16389242607578325
1104	2	0.20561623214643632
1104	3	0.27778906002097514
1104	4	0.1782558925558746
1104	5	0.11562969610063334
1104	6	0.1823207921726464
1104	7	0.17881023407086616
1104	8	0.19232163142286396
1104	9	0.21363408848540644
1104	10-11	0.19715327153343765
1104	12-13	0.22097532344042037
1104	14-15	0.28898235867666244
1104	16-17	0.2831532343796326
1104	18-19	0.26012042267227287
1104	20-21	0.2783016764055688
1104	22-23	0.24643288100921268
1104	24-25	0.24744972957233102
1104	26-27	0.26442530060099756
1104	28-29	0.3208582851309032
1104	30-31	0.2860706866699587
1104	32-33	0.25722670243082746
1104	34-35	0.2863017682297553
1104	36-37	0.25008973759057795
1104	38-39	0.284267276829695
1104	40-41	0.24272764830728022
1104	42-43	0.281521123220827
1104	44-45	0.2612029728220904
1104	46-47	0.3222461035998734
1104	48-49	0.3003561150712031
1104	50-51	0.2609751009268919
1104	52-53	0.24405869089407162
1104	54-55	0.20183229670381309
1104	56-57	0.2145260908845401
1104	58-59	0.3078756886407277
1104	60-61	0.3283784935470706
1104	62-63	0.3230475602951586
1104	64-65	0.25006682898227695
1104	66-67	0.28445811367210894
1104	68-69	0.25503271794116955
1104	70-71	0.3158813568254786
1104	72-73	0.2562484871449513
1104	74-75	0.24334817775687867
1104	76-77	0.23766240742490652
1104	78-79	0.2370830063685503
1104	80-81	0.2373745797129203
1104	82-83	0.2723294187406111
1104	84-85	0.3055258783165513
1104	86-87	0.256456566208378
1104	88-89	0.2826079689704102
1104	90-91	0.28109735465412555
1104	92-93	0.39006288046163107
1104	94-95	0.3836428643778298
1104	96-97	0.4162242818272901
1104	98-99	0.38091316923761553
1104	100	0.36757971446684223
1105	1	0.14630574603110702
1105	2	0.09359008795797408
1105	3	0.2376362876688951
1105	4	0.1740940365989161
1105	5	0.12847597486005924
1105	6	0.10802389450645222
1105	7	0.13773538428016252
1105	8	0.15151800643993596
1105	9	0.16716594244493876
1105	10-11	0.21074196754973684
1105	12-13	0.19528498207086642
1105	14-15	0.24949858096194077
1105	16-17	0.22480476098059654
1105	18-19	0.178611704442325
1105	20-21	0.22802311512969453
1105	22-23	0.21290220175365704
1105	24-25	0.2524812937667136
1105	26-27	0.20417429199958548
1105	28-29	0.23873898155799367
1105	30-31	0.23089647778093791
1105	32-33	0.2741077344669236
1105	34-35	0.24414242477774195
1105	36-37	0.28452611216152235
1105	38-39	0.29594290007099033
1105	40-41	0.3203866037883074
1105	42-43	0.2940690764186513
1105	44-45	0.29856400541440564
1105	46-47	0.3473908377032373
1105	48-49	0.3463142788075544
1105	50-51	0.3576209951771716
1105	52-53	0.339358086146035
1105	54-55	0.33116540089019963
1105	56-57	0.30045302140043617
1105	58-59	0.39971240855436463
1105	60-61	0.37152965795971227
1105	62-63	0.42144483532956656
1105	64-65	0.3609834138469594
1105	66-67	0.34931052175007693
1105	68-69	0.3814531935963359
1105	70-71	0.403153453753184
1105	72-73	0.4697400926466244
1105	74-75	0.39524486840864625
1105	76-77	0.325538948209946
1105	78-79	0.3408254017536265
1105	80-81	0.4095719945820093
1105	82-83	0.4154348913692161
1105	84-85	0.3850390120429026
1105	86-87	0.3827129743923692
1105	88-89	0.4245037355024124
1105	90-91	0.3992649989612893
1105	92-93	0.42643511938222645
1105	94-95	0.4401231311616485
1105	96-97	0.4890883086111195
1105	98-99	0.5623429824133339
1105	100	0.5641302418741603
1106	1	0.1269846001930084
1106	2	0.09124887622503053
1106	3	0.20422725916022344
1106	4	0.12083933472731445
1106	5	0.09463050741851475
1106	6	0.0729360347160366
1106	7	0.08464827953481802
1106	8	0.07497016403844015
1106	9	0.15330228528115697
1106	10-11	0.07733722906989726
1106	12-13	0.11176612882416492
1106	14-15	0.1538709783444503
1106	16-17	0.1403581174991544
1106	18-19	0.1491503604620945
1106	20-21	0.19573063812423896
1106	22-23	0.11785632704538074
1106	24-25	0.11927303860723981
1106	26-27	0.1564442892539688
1106	28-29	0.19791077719246175
1106	30-31	0.20592593597890385
1106	32-33	0.1034658317533399
1106	34-35	0.11897082362458633
1106	36-37	0.11709172100241716
1106	38-39	0.12900844027142
1106	40-41	0.10776305095804162
1106	42-43	0.15114933999801394
1106	44-45	0.14828029150784516
1106	46-47	0.12837885994274245
1106	48-49	0.1710174088125882
1106	50-51	0.16979037695541166
1106	52-53	0.15082304519055612
1106	54-55	0.17438286531156422
1106	56-57	0.17601530117895692
1106	58-59	0.21631939615332385
1106	60-61	0.1326562767305859
1106	62-63	0.12430657056042804
1106	64-65	0.14346493812281125
1106	66-67	0.14781616170797918
1106	68-69	0.1594710858765751
1106	70-71	0.2171827060297602
1106	72-73	0.21590595088118647
1106	74-75	0.18939298308397667
1106	76-77	0.13698274311224878
1106	78-79	0.19127413876914545
1106	80-81	0.21652441530227406
1106	82-83	0.2614454505601742
1106	84-85	0.17300748319103576
1106	86-87	0.25963833933081304
1106	88-89	0.23978939422204348
1106	90-91	0.20516178658770556
1106	92-93	0.3503774842749152
1106	94-95	0.270004897967123
1106	96-97	0.3895118774575046
1106	98-99	0.3346934606085483
1106	100	0.30156388942827306
1107	1	0.09325224881993321
1107	2	0.06867209607798941
1107	3	0.20059073127135818
1107	4	0.16041786696378324
1107	5	0.11335102676819275
1107	6	0.08387324840688848
1107	7	0.07876776880887348
1107	8	0.09659789574771338
1107	9	0.11087546385657987
1107	10-11	0.13671283709384596
1107	12-13	0.0970369019724373
1107	14-15	0.13002527333298985
1107	16-17	0.1144579451989216
1107	18-19	0.14369253191346587
1107	20-21	0.11841440869738307
1107	22-23	0.07626750287681006
1107	24-25	0.10830665610813384
1107	26-27	0.1473023939230913
1107	28-29	0.16179383489190258
1107	30-31	0.148899874098106
1107	32-33	0.15943286299288673
1107	34-35	0.15038121802609083
1107	36-37	0.20550034299710518
1107	38-39	0.15972734190973625
1107	40-41	0.23590801225475388
1107	42-43	0.2742227279570102
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2216	80-81	-0.9994453956949947
2216	82-83	-0.8792456721987847
2216	84-85	-1.022047051818987
2216	86-87	-0.8237947555203888
2216	88-89	-1.1414913724133875
2216	90-91	-0.9611409893295964
2216	92-93	-1.0403442772819353
2216	94-95	-1.025620746406414
2216	96-97	-1.104317760502738
2216	98-99	-1.278810857598664
2216	100	-1.2358424949036504
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	313.0
3	20.0
4	42.0
5	76.0
6	248.0
7	769.0
8	1735.0
9	3070.0
10	4653.0
11	6778.0
12	9300.0
13	12433.0
14	14970.0
15	18897.0
16	23181.0
17	30775.0
18	31442.0
19	19553.0
20	16569.0
21	17396.0
22	19765.0
23	22387.0
24	26114.0
25	31920.0
26	38497.0
27	43251.0
28	54680.0
29	71782.0
30	93351.0
31	119768.0
32	154639.0
33	201887.0
34	273277.0
35	393406.0
36	640508.0
37	1139351.0
38	1517590.0
39	569184.0
40	2042.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.94056676061167	20.783113301442178	14.394497522273927	23.881822415672225
2	15.616272627065571	20.515537934581065	39.38617243720202	24.48201700115134
3	17.780496688453308	25.012394191643622	31.24289078232991	25.964218337573165
4	12.759351644220231	17.42900998379733	37.506797034635326	32.30484133734712
5	12.765546333656793	37.56918127587382	35.72589967432917	13.939372716140214
6	30.91119395039017	37.93356784382305	17.057447367125288	14.097790838661487
7	26.47342452448344	32.89211373895033	22.187656149483285	18.446805587082952
8	24.85454386117411	36.37259382545334	20.241614240951908	18.531248072420635
9	24.5577064497258	16.405359125813533	21.406906511087936	37.63002791337273
10-11	23.847642366111177	26.84674344281047	28.637986326482473	20.66762786459588
12-13	24.46186258623216	24.22292356369834	28.932979702631528	22.38223414743797
14-15	21.775555837203875	26.398945323825156	26.296369835607248	25.52912900336372
16-17	21.012038144075902	29.81914164808204	27.10347556481795	22.06534464302411
18-19	20.913756014786294	28.11003524858747	29.78140791883128	21.19480081779496
20-21	21.800450101859965	27.316370981984107	29.624429540198754	21.258749375957173
22-23	21.534145842439738	27.34675952992906	29.66525105948341	21.45384356814779
24-25	21.595192768924466	27.47286804618558	29.568739097290543	21.363200087599413
26-27	21.306593610331888	27.444164751467977	29.733790788666052	21.515450849534087
28-29	21.13271097612504	27.250404001279154	29.975615049840897	21.641269972754912
30-31	21.60698611245911	27.463516008374544	29.70211450546411	21.22738337370223
32-33	21.192870493163714	27.47410434768233	29.879729412286483	21.45329574686747
34-35	21.326032798191843	27.211389332544083	30.04027115257621	21.422306716687864
36-37	21.686444798795794	27.28154812571176	29.445755757747754	21.586251317744697
38-39	21.410001987342866	27.169216734493485	29.727929562027317	21.692851716136335
40-41	21.283252610249804	26.861482246106245	30.0009101233626	21.854355020281353
42-43	21.276071922741643	26.878460080290218	29.965370755646692	21.880097241321447
44-45	21.17016747532043	27.10330711096851	29.783143324140248	21.94338208957081
46-47	21.37227914047396	27.126346431286052	29.34819358320481	22.153180845035173
48-49	21.22203319464826	27.11811440447293	29.204532051876292	22.455320349002516
50-51	21.019787330921204	27.181429447145543	29.170836966653034	22.62794625528022
52-53	20.944044461173576	27.237650501081447	29.347947523656437	22.47035751408854
54-55	20.982804572921136	27.481193320990343	28.959330431654823	22.576671674433697
56-57	21.035184509206285	27.32332886535475	28.70562029399068	22.935866331448285
58-59	20.782558482753384	27.176858906805062	29.01784099381907	23.022741616622476
60-61	21.071695240898745	27.60941234733546	28.345261372076862	22.97363103968894
62-63	20.704642761787508	27.748412873068595	28.33466416049278	23.21228020465112
64-65	20.704738024109098	27.514356706154054	28.35936969500465	23.421535574732196
66-67	20.847001279254858	27.55719206003405	28.06198799919759	23.533818661513504
68-69	21.095405465339564	27.64389390001053	27.825823020624235	23.43487761402567
70-71	21.07237170959308	27.375863819584627	27.832873174682295	23.71889129614
72-73	20.880956298016816	27.385276705812046	27.890541195969792	23.84322580020135
74-75	20.992756024876762	27.322059634296714	27.84102024123238	23.84416409959415
76-77	21.042764997470485	27.32511053967394	27.693188011480412	23.938936451375163
78-79	21.122022038766314	27.348189505161454	27.685291365972258	23.844497090099974
80-81	20.998261481686896	27.153016275944452	27.780678271009762	24.06804397135889
82-83	20.84514292880575	27.1041626096527	27.92649175088887	24.124202710652682
84-85	20.93174726254179	26.937116765627216	27.84679122150418	24.284344750326817
86-87	21.072339150194033	27.038038816875098	27.77388778292158	24.11573425000929
88-89	20.950381813728086	26.824000918670954	27.831454810028163	24.394162457572797
90-91	20.889536815343508	26.537512352307818	27.866779771350537	24.706171060998138
92-93	20.87628410119205	26.523231833577267	27.92100551748326	24.679478547747426
94-95	20.74210090231469	26.26862060635106	28.22561191420162	24.763666577132625
96-97	20.77890726866941	26.27517467507015	28.19365401314918	24.752264043111253
98-99	20.82474404567159	26.327381026977942	28.112050796142864	24.735824131207607
100	20.886669647118637	26.308899848814647	28.073137802648457	24.731292701418262
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	583.0
1	425.0
2	459.5
3	663.5
4	449.5
5	301.0
6	783.0
7	1889.0
8	3072.0
9	3617.5
10	2938.5
11	2060.0
12	2040.0
13	2330.0
14	2742.0
15	3129.5
16	3559.5
17	4139.5
18	4752.0
19	5663.0
20	6915.0
21	8615.0
22	10878.0
23	14141.0
24	18798.0
25	24776.5
26	32966.0
27	43551.0
28	56527.0
29	71485.5
30	86104.5
31	98939.0
32	110667.5
33	122330.0
34	133932.0
35	145170.5
36	156803.0
37	168667.5
38	179685.0
39	187255.5
40	193604.0
41	201631.0
42	207352.5
43	210829.5
44	212227.0
45	210669.5
46	208386.5
47	203898.0
48	197359.5
49	191853.0
50	186881.0
51	180321.5
52	170264.0
53	160862.5
54	152949.5
55	143935.5
56	131715.5
57	117121.5
58	103995.0
59	92383.5
60	80629.0
61	68224.0
62	57800.0
63	48125.0
64	38893.0
65	31341.0
66	24784.5
67	19062.0
68	14512.5
69	11156.5
70	8379.0
71	6208.0
72	4551.0
73	3241.5
74	2335.5
75	1667.0
76	1134.5
77	731.5
78	479.5
79	293.5
80	153.5
81	70.0
82	41.5
83	23.5
84	11.0
85	4.5
86	1.5
87	2.0
88	2.0
89	1.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03761363860581387
2	0.0
3	0.0
4	7.110328658944021E-5
5	0.0
6	0.0
7	0.0
8	7.11032865894402E-4
9	0.0
10-11	0.0
12-13	0.043141919138142845
14-15	1.066549298841603E-4
16-17	1.7775821647360052E-5
18-19	6.221537576576018E-5
20-21	6.221537576576018E-5
22-23	0.0
24-25	1.8664612729728052E-4
26-27	1.066549298841603E-4
28-29	3.1996478965248094E-4
30-31	1.7775821647360052E-5
32-33	2.2219777059200065E-4
34-35	7.110328658944021E-5
36-37	6.221537576576018E-5
38-39	3.021889680051209E-4
40-41	1.4220657317888042E-4
42-43	4.7994718447872135E-4
44-45	2.4886150306304073E-4
46-47	1.2443075153152036E-4
48-49	7.732482416601622E-4
50-51	4.355076303603212E-4
52-53	6.488174901286419E-4
54-55	4.355076303603212E-4
56-57	5.777142035392017E-4
58-59	1.333186623552004E-4
60-61	1.5109448400256044E-4
62-63	7.999119741312022E-4
64-65	4.443955411840013E-4
66-67	0.0013954019993177638
68-69	0.0013598503560230438
70-71	4.088438978892812E-4
72-73	8.976789931916826E-4
74-75	8.443515282496024E-4
76-77	6.577054009523219E-4
78-79	0.0029863380367564883
80-81	0.002470839208983047
82-83	0.0013065228910809636
84-85	0.0025774941388672073
86-87	0.001350962445199364
88-89	0.0017953579863833652
90-91	0.002701924890398728
92-93	0.0024441754765120068
94-95	0.0035196126861772896
96-97	0.0030929929666406487
98-99	0.002710812801222408
100	0.0013154108019046437
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5625619.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.92322925393275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.25380465921036	47.83805056650821
2	13.642897747995608	16.8962456987419
3	4.157740601251491	7.723821732890408
4	1.7511209188099262	4.337402484272977
5	0.9228304717468222	2.8572321432246692
6	0.5296361744822977	1.9678069352185947
7	0.35591199284222136	1.5427453948895062
8	0.23168631227322295	1.147741170391443
9	0.18399477931706454	1.0254195811059619
>10	0.9109031198592016	9.865768065773917
>50	0.044540153428072465	1.8803063778124827
>100	0.014015245796914858	1.4132328281555393
>500	5.162775369188299E-4	0.23964686356618428
>1k	2.581363606727241E-4	0.34091678662305247
>5k	5.736363569147983E-5	0.24646534240166695
>10k+	8.604545353721974E-5	0.677198028423518
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	16524	0.29372767690097745	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10929	0.19427195478399797	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10676	0.18977467190721592	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	7913	0.14066007669556008	TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5964	0.10601500030485533	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.7775821647360052E-5	0.0
4	0.0	0.0	0.0	5.332746494208015E-5	0.0
5	0.0	0.0	0.0	7.110328658944021E-5	0.0
6	0.0	0.0	0.0	8.887910823680025E-5	0.0
7	0.0	0.0	0.0	1.066549298841603E-4	0.0
8	0.0	0.0	0.0	1.066549298841603E-4	0.0
9	0.0	0.0	0.0	1.9553403812096055E-4	0.0
10-11	0.0	0.0	0.0	2.666373247104008E-4	0.0
12-13	0.0	8.887910823680026E-6	0.0	3.021889680051209E-4	0.0
14-15	0.0	1.7775821647360052E-5	0.0	3.55516432947201E-4	0.0
16-17	0.0	1.7775821647360052E-5	0.0	4.1773180871296114E-4	0.0
18-19	0.0	1.7775821647360052E-5	0.0	4.977230061260815E-4	0.0
20-21	0.0	2.6663732471040078E-5	0.0	7.910240633075222E-4	0.0
22-23	0.0	3.5551643294720104E-5	0.0	0.0023819601007462467	0.0
24-25	0.0	3.5551643294720104E-5	0.0	0.005323858583384335	0.0
26-27	0.0	3.5551643294720104E-5	0.0	0.011145440172894752	0.0
28-29	0.0	3.5551643294720104E-5	0.0	0.03702703649145098	0.0
30-31	1.7775821647360052E-5	3.5551643294720104E-5	0.0	0.11251206311696543	0.0
32-33	1.7775821647360052E-5	3.5551643294720104E-5	0.0	0.27812939340541903	0.0
34-35	1.7775821647360052E-5	3.5551643294720104E-5	0.0	0.5400027979143274	0.0
36-37	1.7775821647360052E-5	3.5551643294720104E-5	0.0	0.9382078665476635	0.0
38-39	1.7775821647360052E-5	3.5551643294720104E-5	0.0	1.4377173427493046	0.0
40-41	1.7775821647360052E-5	3.5551643294720104E-5	0.0	1.923966056002015	0.0
42-43	1.7775821647360052E-5	3.5551643294720104E-5	0.0	2.389452609570609	0.0
44-45	1.7775821647360052E-5	3.5551643294720104E-5	0.0	2.9497287320737504	0.0
46-47	1.7775821647360052E-5	5.332746494208015E-5	0.0	3.584343340706152	0.0
48-49	1.7775821647360052E-5	5.332746494208015E-5	0.0	4.206292676414808	0.0
50-51	1.7775821647360052E-5	7.110328658944021E-5	0.0	4.791694922816493	0.0
52-53	1.7775821647360052E-5	7.999119741312023E-5	0.0	5.33611501241019	0.0
54-55	1.7775821647360052E-5	8.887910823680025E-5	0.0	5.919046419602891	0.0
56-57	1.7775821647360052E-5	8.887910823680025E-5	0.0	6.57546307348578	0.0
58-59	1.7775821647360052E-5	8.887910823680025E-5	0.0	7.2535484539568	0.0
60-61	1.7775821647360052E-5	8.887910823680025E-5	0.0	7.911671586717835	0.0
62-63	1.7775821647360052E-5	8.887910823680025E-5	0.0	8.523435376622555	0.0
64-65	1.7775821647360052E-5	8.887910823680025E-5	0.0	9.15102853570425	0.0
66-67	1.7775821647360052E-5	8.887910823680025E-5	0.0	9.828251788825373	0.0
68-69	1.7775821647360052E-5	8.887910823680025E-5	0.0	10.540475634770146	0.0
70-71	1.7775821647360052E-5	8.887910823680025E-5	0.0	11.250993001836775	0.0
72-73	1.7775821647360052E-5	1.1554284070784034E-4	0.0	11.937966293131476	0.0
74-75	1.7775821647360052E-5	1.2443075153152036E-4	0.0	12.60178657673049	0.0
76-77	1.7775821647360052E-5	1.2443075153152036E-4	0.0	13.291666214864534	0.0
78-79	1.7775821647360052E-5	1.2443075153152036E-4	0.0	14.024074506289885	0.0
80-81	1.7775821647360052E-5	1.2443075153152036E-4	0.0	14.767112739060359	0.0
82-83	1.7775821647360052E-5	1.2443075153152036E-4	0.0	15.492695114973126	0.0
84-85	1.7775821647360052E-5	1.2443075153152036E-4	0.0	16.182672520126232	0.0
86-87	1.7775821647360052E-5	1.333186623552004E-4	0.0	16.882142214039025	0.0
88	1.7775821647360052E-5	1.4220657317888042E-4	0.0	17.42170950432299	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8720	0.0	56.93026	1
GTATCAA	14855	0.0	37.152767	1
GTGGTAT	3590	0.0	31.427692	1
ATCAACG	18215	0.0	29.748734	3
TCAACGC	18275	0.0	29.573912	4
CAACGCA	18935	0.0	28.543083	5
TATCAAC	19285	0.0	28.244385	2
AACGCAG	19790	0.0	27.377851	6
TGGTATC	4230	0.0	23.998367	2
GTACATG	14445	0.0	23.79006	1
ACGCAGA	22775	0.0	23.624376	7
CGCAGAG	23190	0.0	23.019072	8
TACATGG	14435	0.0	22.660046	2
ACATGGG	14910	0.0	21.33926	3
GCAGAGT	24895	0.0	21.06452	9
GAGTACT	17190	0.0	20.703634	12-13
CAGAGTA	24400	0.0	19.949837	10-11
AGAGTAC	23180	0.0	19.16361	10-11
GTACTTT	18775	0.0	18.711111	14-15
CATGGGG	10960	0.0	18.610048	4
>>END_MODULE