FastQCFastQC Report
Wed 25 May 2016
SRR2049344_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049344_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5611676
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49100.041.1703641
GTATCAA86300.036.1705471
TCAACGC88350.033.3610274
ATCAACG90450.032.5345043
CAACGCA93700.031.6067165
AACGCAG99900.029.6414386
TATCAAC104950.028.7561682
ACGCAGA117250.025.0147367
GTACATG142300.024.6451631
CGCAGAG117950.024.3880838
TACATGG141850.023.3303742
ACATGGG147700.021.4833353
GCAGAGT141000.019.5011839
GAGTACA107250.018.716661
GTGGTAT30350.018.5874711
CATGGGG108700.018.0769484
GTCTACG6500.017.357841
AGAGTAC117100.017.21966210-11
CAGAGTA129250.016.928310-11
AGTACAT111400.016.0774882
Produced by FastQC (version 0.11.5)