##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049344_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5611676
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09942252546298	31.0	31.0	34.0	28.0	34.0
2	31.204078246855307	31.0	31.0	34.0	28.0	34.0
3	31.364039014369325	33.0	31.0	34.0	28.0	34.0
4	34.96488001089158	37.0	35.0	37.0	32.0	37.0
5	34.96567977196118	37.0	35.0	37.0	32.0	37.0
6	34.99478480225872	37.0	35.0	37.0	32.0	37.0
7	34.87834597008095	37.0	35.0	37.0	32.0	37.0
8	34.86003219002665	37.0	35.0	37.0	32.0	37.0
9	36.47509799211501	39.0	37.0	39.0	32.0	39.0
10-11	36.45220803909562	39.0	37.0	39.0	32.0	39.0
12-13	36.407116608300264	39.0	37.0	39.0	32.0	39.0
14-15	37.646615734764445	40.0	38.0	41.0	32.0	41.0
16-17	37.61549811500165	40.0	38.0	41.0	32.0	41.0
18-19	37.64324936079703	40.0	38.0	41.0	32.0	41.0
20-21	37.71995835112362	40.0	38.0	41.0	32.0	41.0
22-23	37.67169665533078	40.0	38.0	41.0	32.0	41.0
24-25	37.67880932541365	40.0	38.0	41.0	32.0	41.0
26-27	37.667105780875445	40.0	38.0	41.0	32.0	41.0
28-29	37.60826382349943	40.0	38.0	41.0	32.0	41.0
30-31	37.547782605410575	40.0	38.0	41.0	32.0	41.0
32-33	37.49633309193189	40.0	38.0	41.0	32.0	41.0
34-35	37.39684614721163	40.0	38.0	41.0	32.0	41.0
36-37	37.31888467901568	40.0	37.0	41.0	31.5	41.0
38-39	37.219356837422545	40.0	37.0	41.0	31.0	41.0
40-41	37.115860573561264	40.0	37.0	41.0	31.0	41.0
42-43	36.977585484265305	40.0	37.0	41.0	31.0	41.0
44-45	36.85907409836206	40.0	37.0	41.0	30.0	41.0
46-47	36.743082636987594	40.0	36.5	41.0	30.0	41.0
48-49	36.61293310590277	40.0	36.0	41.0	30.0	41.0
50-51	36.47268106711792	40.0	36.0	41.0	30.0	41.0
52-53	36.32819838493883	39.5	35.5	41.0	30.0	41.0
54-55	36.18725849461016	39.0	35.0	41.0	30.0	41.0
56-57	36.0581153473579	39.0	35.0	41.0	29.0	41.0
58-59	35.84705264523468	39.0	35.0	41.0	29.0	41.0
60-61	35.62991885133782	39.0	35.0	40.5	28.5	41.0
62-63	35.4172071766082	38.0	35.0	40.0	28.0	41.0
64-65	35.16718472342309	38.0	34.5	40.0	28.0	41.0
66-67	34.9028504140296	37.5	34.0	40.0	28.0	41.0
68-69	34.60906278266956	37.0	34.0	40.0	28.0	41.0
70-71	34.32049551684737	36.5	34.0	39.0	28.0	41.0
72-73	33.972355674133716	36.0	34.0	39.0	26.5	41.0
74-75	33.61781310966634	35.5	34.0	39.0	26.0	40.5
76-77	33.27572128184165	35.0	33.0	37.5	26.0	40.0
78-79	32.95237171568708	35.0	33.0	37.0	26.0	39.0
80-81	32.59116536663913	35.0	33.0	37.0	26.0	39.0
82-83	32.27052301308913	35.0	33.0	36.0	25.0	39.0
84-85	31.972412698095898	35.0	33.0	36.0	25.0	37.0
86-87	31.649029452163667	35.0	33.0	35.5	24.0	37.0
88-89	31.389665582973784	35.0	32.0	35.0	23.5	36.5
90-91	31.17840570268134	35.0	32.0	35.0	22.0	36.0
92-93	30.939263065080738	35.0	32.0	35.0	20.0	36.0
94-95	30.733668782730863	35.0	32.0	35.0	19.0	36.0
96-97	30.476225640967154	35.0	32.0	35.0	12.5	35.5
98-99	30.266936027668024	35.0	32.0	35.0	2.0	35.0
100	30.146332218752473	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4031703545283314
1101	2	0.35453358851468764
1101	3	0.39428771691464704
1101	4	0.40139072026743605
1101	5	0.367071895011442
1101	6	0.4643239756243318
1101	7	0.4092195269109169
1101	8	0.495620565186627
1101	9	0.6268923477716868
1101	10-11	0.6246928730242871
1101	12-13	0.6886966541775408
1101	14-15	0.7748927654985138
1101	16-17	0.769650125022217
1101	18-19	0.7613757301640334
1101	20-21	0.7085891901532406
1101	22-23	0.687923616723225
1101	24-25	0.6755366057151946
1101	26-27	0.6510018223538268
1101	28-29	0.6519707301725433
1101	30-31	0.6821558094895792
1101	32-33	0.638833177678336
1101	34-35	0.7009426268881143
1101	36-37	0.6930964140600722
1101	38-39	0.7346961686070657
1101	40-41	0.8028281416753842
1101	42-43	0.7291850762735876
1101	44-45	0.6498101378285881
1101	46-47	0.6584392752149739
1101	48-49	0.6275971612591533
1101	50-51	0.6631758288892939
1101	52-53	0.6762370103901887
1101	54-55	0.6634390367966958
1101	56-57	0.5848635455346667
1101	58-59	0.6798366203424493
1101	60-61	0.7140012722233422
1101	62-63	0.7308500086126131
1101	64-65	0.6833154220331537
1101	66-67	0.6518120220303203
1101	68-69	0.7447220329592952
1101	70-71	0.7616721808484854
1101	72-73	0.798819895596246
1101	74-75	0.7486825304050413
1101	76-77	0.7805533872246571
1101	78-79	0.7424182538595048
1101	80-81	0.7570732446030135
1101	82-83	0.754498961106691
1101	84-85	0.6905696222930047
1101	86-87	0.7581327440548407
1101	88-89	0.7769500846814523
1101	90-91	0.7524666618209643
1101	92-93	0.7641962606006878
1101	94-95	0.7592415741235676
1101	96-97	0.780877196422253
1101	98-99	0.8797278121878485
1101	100	0.9168966393773808
1102	1	0.24550939033976604
1102	2	0.30688135279813267
1102	3	0.3585257479949213
1102	4	0.36902627315299696
1102	5	0.2820493033605018
1102	6	0.432840289013356
1102	7	0.48018317644392283
1102	8	0.45971622304164583
1102	9	0.5379237497426885
1102	10-11	0.4507340484205429
1102	12-13	0.5347038368074237
1102	14-15	0.6267035360528723
1102	16-17	0.5685083447317751
1102	18-19	0.5469791102103514
1102	20-21	0.5255657685657269
1102	22-23	0.5113642292750882
1102	24-25	0.5889187303528161
1102	26-27	0.49666611728121524
1102	28-29	0.5692071877306901
1102	30-31	0.5539614840757707
1102	32-33	0.5210261544534944
1102	34-35	0.5798441002457082
1102	36-37	0.5637987872032042
1102	38-39	0.5458830073744778
1102	40-41	0.5712821783668787
1102	42-43	0.5521375307681211
1102	44-45	0.49021545552815127
1102	46-47	0.4145463333245729
1102	48-49	0.48409694432208994
1102	50-51	0.5214494664536815
1102	52-53	0.4980021549612559
1102	54-55	0.478227769167745
1102	56-57	0.4253487164030574
1102	58-59	0.5382491711316106
1102	60-61	0.451117306534357
1102	62-63	0.4393096816283304
1102	64-65	0.4114046462076715
1102	66-67	0.522305073422288
1102	68-69	0.5328284088031552
1102	70-71	0.5631449772321275
1102	72-73	0.545974144536423
1102	74-75	0.5217913851985188
1102	76-77	0.5772961229122799
1102	78-79	0.5168607318979426
1102	80-81	0.47277204980691323
1102	82-83	0.4813720447796328
1102	84-85	0.49441275904931814
1102	86-87	0.5492823310334245
1102	88-89	0.5383155571479961
1102	90-91	0.6129803848119373
1102	92-93	0.6046161715558398
1102	94-95	0.6354951869269314
1102	96-97	0.6148380066358285
1102	98-99	0.645881561100019
1102	100	0.652659267187147
1103	1	0.18308361167657594
1103	2	0.27419875033332985
1103	3	0.27560016676959265
1103	4	0.23707537863879224
1103	5	0.2092150681102325
1103	6	0.32874780408823057
1103	7	0.30183763097851113
1103	8	0.3471643983743249
1103	9	0.3881721039573023
1103	10-11	0.4200409658158719
1103	12-13	0.43900134446562333
1103	14-15	0.47820027375092167
1103	16-17	0.5087696516684375
1103	18-19	0.516185479372723
1103	20-21	0.492426030770055
1103	22-23	0.4546802876665197
1103	24-25	0.5189956899402333
1103	26-27	0.5380550231168897
1103	28-29	0.49381957219406303
1103	30-31	0.5257306255229466
1103	32-33	0.5335751789432024
1103	34-35	0.5500985324929104
1103	36-37	0.5245186780241582
1103	38-39	0.547679943752108
1103	40-41	0.598183356424407
1103	42-43	0.5986553942858137
1103	44-45	0.48532765680796075
1103	46-47	0.5060239151469332
1103	48-49	0.5182648525380955
1103	50-51	0.5289950106773844
1103	52-53	0.4848319440109492
1103	54-55	0.4981530047421927
1103	56-57	0.5160009506248642
1103	58-59	0.6094628431825342
1103	60-61	0.5258249128747394
1103	62-63	0.5494068449407052
1103	64-65	0.5225133420072154
1103	66-67	0.603277792090033
1103	68-69	0.6063258252591908
1103	70-71	0.4836247794306203
1103	72-73	0.5323562932013957
1103	74-75	0.4963889040130809
1103	76-77	0.5476029001086715
1103	78-79	0.47167027610694134
1103	80-81	0.47013071258398753
1103	82-83	0.4527238520630448
1103	84-85	0.5242638877964723
1103	86-87	0.48557057713583873
1103	88-89	0.5066224642802339
1103	90-91	0.5467710641229893
1103	92-93	0.485147155869587
1103	94-95	0.4986337422494138
1103	96-97	0.5552035045704429
1103	98-99	0.5362204605149152
1103	100	0.49440043473324025
1104	1	0.1396604417414764
1104	2	0.18458238286183004
1104	3	0.19274357188305302
1104	4	0.2117281173572323
1104	5	0.22999896540267883
1104	6	0.2586612778404387
1104	7	0.2811342394991385
1104	8	0.30570740489348935
1104	9	0.34221891513049485
1104	10-11	0.3135689200412628
1104	12-13	0.28500253503435147
1104	14-15	0.3969566013629944
1104	16-17	0.4230401941689834
1104	18-19	0.35389019587880455
1104	20-21	0.3028304958372914
1104	22-23	0.3488360846402685
1104	24-25	0.40849340487868346
1104	26-27	0.33699652254791346
1104	28-29	0.4381663683040955
1104	30-31	0.39325179239616403
1104	32-33	0.39344180603689693
1104	34-35	0.47161550340798897
1104	36-37	0.39837822929658273
1104	38-39	0.4915787376857068
1104	40-41	0.48666850255875005
1104	42-43	0.4344544645704147
1104	44-45	0.3807168704291044
1104	46-47	0.3349106501063446
1104	48-49	0.2981362810129653
1104	50-51	0.3144144604473169
1104	52-53	0.3148740942958739
1104	54-55	0.36434751547685806
1104	56-57	0.31428011774817577
1104	58-59	0.40484440452699744
1104	60-61	0.33382560954574103
1104	62-63	0.28437769507726784
1104	64-65	0.3634952606671362
1104	66-67	0.4350155432573004
1104	68-69	0.35935481121424573
1104	70-71	0.32944416807889354
1104	72-73	0.38591142145789803
1104	74-75	0.48757789279731156
1104	76-77	0.44861765355328487
1104	78-79	0.3665196662663561
1104	80-81	0.3981101156484712
1104	82-83	0.37455095744093114
1104	84-85	0.41840179123681054
1104	86-87	0.4695398420199979
1104	88-89	0.4781275403708989
1104	90-91	0.5084537973520717
1104	92-93	0.4990244811840405
1104	94-95	0.5279305559572727
1104	96-97	0.5205181121148641
1104	98-99	0.5323896752851098
1104	100	0.5184494643424813
1105	1	0.16752128918660247
1105	2	0.17918513513115286
1105	3	0.2534547232263229
1105	4	0.2629223027742995
1105	5	0.2632830367611021
1105	6	0.31063324118989755
1105	7	0.2959059900294463
1105	8	0.3685998018039882
1105	9	0.38512942347047385
1105	10-11	0.3715630380594064
1105	12-13	0.3199512854176163
1105	14-15	0.39262458005881484
1105	16-17	0.4471389018075129
1105	18-19	0.3772823571482178
1105	20-21	0.3882209340972338
1105	22-23	0.3868226568092936
1105	24-25	0.34432826199344646
1105	26-27	0.34702495255206856
1105	28-29	0.4518161854735183
1105	30-31	0.3943878445080955
1105	32-33	0.40115513775599254
1105	34-35	0.4315279335216218
1105	36-37	0.42138840152330914
1105	38-39	0.43226670528598987
1105	40-41	0.4677580155563561
1105	42-43	0.44609680987644396
1105	44-45	0.4045038638761511
1105	46-47	0.39480137735132814
1105	48-49	0.424473677531509
1105	50-51	0.4326430552610745
1105	52-53	0.42891933506493274
1105	54-55	0.43085657397476496
1105	56-57	0.431619637123859
1105	58-59	0.42256613327906223
1105	60-61	0.39819337245370434
1105	62-63	0.3351028306520618
1105	64-65	0.3949830616023675
1105	66-67	0.49726343512772786
1105	68-69	0.4207869755729732
1105	70-71	0.396259345452151
1105	72-73	0.401350232240425
1105	74-75	0.45896051802453997
1105	76-77	0.5567407015707175
1105	78-79	0.42225782820095503
1105	80-81	0.48022987419985697
1105	82-83	0.4070177647500728
1105	84-85	0.43861865409323997
1105	86-87	0.4855059171254936
1105	88-89	0.450454875293417
1105	90-91	0.45472936939631836
1105	92-93	0.46861005322364946
1105	94-95	0.42589611061139365
1105	96-97	0.42748412413534354
1105	98-99	0.45262116713902856
1105	100	0.5275551600379984
1106	1	0.08151688588267803
1106	2	0.0832874133082484
1106	3	0.18009074623158483
1106	4	0.19766983872884225
1106	5	0.1268682476824523
1106	6	0.22864649143112104
1106	7	0.27163286957512156
1106	8	0.19890019236111556
1106	9	0.3240365222268764
1106	10-11	0.31502207157083006
1106	12-13	0.36049192019645915
1106	14-15	0.3767431362106848
1106	16-17	0.4093840658862078
1106	18-19	0.3864364704329688
1106	20-21	0.3475733002429493
1106	22-23	0.38292477333250474
1106	24-25	0.32502032714029383
1106	26-27	0.2853092973648472
1106	28-29	0.3742366045435688
1106	30-31	0.3548065885616083
1106	32-33	0.36823517289639085
1106	34-35	0.38963130496291853
1106	36-37	0.36618599472887325
1106	38-39	0.3836243458517643
1106	40-41	0.39359421312092735
1106	42-43	0.35679475976239416
1106	44-45	0.34875810174530386
1106	46-47	0.34273626578830374
1106	48-49	0.37224203894254515
1106	50-51	0.3903877466449899
1106	52-53	0.41146416040984946
1106	54-55	0.4362852541968252
1106	56-57	0.32579561905357224
1106	58-59	0.3840914586621196
1106	60-61	0.39211559811512586
1106	62-63	0.40160802827814024
1106	64-65	0.4378873803152672
1106	66-67	0.43851207862983443
1106	68-69	0.41575520436732916
1106	70-71	0.3801709611071047
1106	72-73	0.4166358924654716
1106	74-75	0.4340361609516137
1106	76-77	0.4480440923029434
1106	78-79	0.3383204353050573
1106	80-81	0.3804390160126587
1106	82-83	0.33143558391483197
1106	84-85	0.3406592991224855
1106	86-87	0.32466992288857455
1106	88-89	0.33302500736900953
1106	90-91	0.3832924260057631
1106	92-93	0.389957288790999
1106	94-95	0.36670541515352895
1106	96-97	0.3337248714669627
1106	98-99	0.4016846500643787
1106	100	0.44617259587132096
1107	1	0.04439241251304793
1107	2	0.024899381324743786
1107	3	0.0676224007855204
1107	4	0.08314268373426614
1107	5	0.08670772067074495
1107	6	0.14837808786003848
1107	7	0.12064650076363392
1107	8	0.19183054266947153
1107	9	0.18967122098099054
1107	10-11	0.118010915307309
1107	12-13	0.1219883845485299
1107	14-15	0.19638350178603758
1107	16-17	0.22501941222727595
1107	18-19	0.18536155634912177
1107	20-21	0.17743468996837208
1107	22-23	0.16763167942023927
1107	24-25	0.17425428754732053
1107	26-27	0.15524294636689007
1107	28-29	0.2114928115217225
1107	30-31	0.2508889486710544
1107	32-33	0.2588860130275492
1107	34-35	0.26920997264308966
1107	36-37	0.25047167406977167
1107	38-39	0.29325081714961243
1107	40-41	0.37286228122562903
1107	42-43	0.2751974560532773
1107	44-45	0.21720519175480035
1107	46-47	0.2650331976872664
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2215	100	-0.2629682423058348
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2216	2	-1.2303992673867619
2216	3	-1.3104950874367454
2216	4	-1.5256810840781867
2216	5	-1.4015521485577978
2216	6	-2.834388595871502
2216	7	-2.656582332070471
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2216	9	-3.0233736720794155
2216	10-11	-2.2307777862339293
2216	12-13	-2.015535385535543
2216	14-15	-2.264254021722728
2216	16-17	-2.6417524274051587
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2216	32-33	-2.6548093159006285
2216	34-35	-3.6759981763999434
2216	36-37	-2.711184612905903
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2216	54-55	-2.7694460653730246
2216	56-57	-2.706771059998246
2216	58-59	-3.798932550872671
2216	60-61	-4.614762889071013
2216	62-63	-3.664699683268413
2216	64-65	-3.631933317742444
2216	66-67	-3.2668701929660244
2216	68-69	-3.643191095640809
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2216	76-77	-5.018590805072677
2216	78-79	-3.9704183894059355
2216	80-81	-3.801800939233118
2216	82-83	-4.0034468807380215
2216	84-85	-4.049858666183045
2216	86-87	-3.708933158356423
2216	88-89	-3.6687353922483013
2216	90-91	-3.8475608498710265
2216	92-93	-3.8967669540323193
2216	94-95	-3.7781365586352784
2216	96-97	-4.0216385527760785
2216	98-99	-3.758746084559821
2216	100	-3.406656442155331
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68256.0
3	12576.0
4	8021.0
5	6772.0
6	6344.0
7	6676.0
8	7530.0
9	8411.0
10	9780.0
11	11055.0
12	12631.0
13	14121.0
14	15624.0
15	16588.0
16	16818.0
17	17803.0
18	17071.0
19	16014.0
20	15657.0
21	16514.0
22	18358.0
23	21789.0
24	26652.0
25	33823.0
26	41437.0
27	49469.0
28	60426.0
29	76852.0
30	94240.0
31	112301.0
32	142760.0
33	191338.0
34	265817.0
35	386919.0
36	641650.0
37	1126333.0
38	1476142.0
39	539330.0
40	1778.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.710628259980346	20.00481227236392	13.804288447605492	23.480271020050246
2	15.639473991494658	20.049167337413973	40.385530398446186	23.925828272645187
3	18.481786641760486	24.855664245753985	30.252453195282065	26.41009591720347
4	12.649518982720032	16.489367202255455	38.59706829229506	32.264045522729454
5	13.135542302926574	37.70998136906194	35.612155572789916	13.542320755221565
6	31.46604439747356	37.52097466495803	17.48085448984409	13.532126447724323
7	27.276357628125453	32.38788020112428	22.42802447663403	17.90773769411624
8	24.864448085230038	36.523748994970845	20.416327200055623	18.1954757197435
9	24.803463975838596	16.897721399745457	21.55812594029498	36.74068868412096
10-11	23.929964124370155	26.893217877396747	28.94271139608141	20.234106602151684
12-13	24.247072459768468	24.432090310389817	28.981919577136694	22.33891765270502
14-15	21.679071872345634	26.705089956868733	26.040761264631247	25.575076906154393
16-17	21.331746259393356	29.980598038910706	26.771773769942676	21.915881931753265
18-19	21.256391494106094	28.999393062658953	28.655295512701606	21.08891993053335
20-21	22.300724004680752	28.259928590733054	28.254580383202637	21.18476702138356
22-23	21.96650839937411	28.115061682527564	28.27048250517401	21.647947412924317
24-25	21.85467611022075	28.22779157389201	28.205258250779487	21.712274065107756
26-27	21.58937566277541	28.137215691241607	28.363627839076504	21.909780806906475
28-29	21.619994535989157	28.202425111631168	28.430264558847977	21.747315793531698
30-31	21.878640912998698	28.57648128975729	28.01168211907543	21.533195678168582
32-33	21.595838586012025	28.30321104274322	28.35806500809929	21.74288536314546
34-35	21.740494205578553	28.046249478781064	28.41046541664446	21.802790898995923
36-37	21.956290303661472	28.40054428103812	27.974622798551486	21.668542616748923
38-39	21.84503057363923	28.470492663342707	28.083359672245884	21.60111709077218
40-41	21.689653091586578	28.199450343804756	28.285901799026348	21.82499476558232
42-43	21.654738864609204	28.193086619722013	28.242921600955235	21.909252914713548
44-45	21.7371540054004	28.283239645542658	28.193696543543567	21.78590980551338
46-47	21.704749435592678	28.40068949098477	28.04088957136418	21.853671502058376
48-49	21.68125020611858	28.27764194996644	28.09155141109894	21.94955643281604
50-51	21.684908582442926	28.20522990874957	28.06636881218455	22.043492696622955
52-53	21.688053500420533	28.171328557885282	28.12910573905888	22.011512202635302
54-55	21.740823188870838	28.476853244556754	27.935146746529938	21.84717682004247
56-57	21.690323853790854	28.281613030626634	27.94003361869441	22.0880294968881
58-59	21.57259437445161	27.90089064824689	28.29244109496148	22.23407388234002
60-61	21.789715166236515	28.28877446717786	27.910453721064638	22.011056645520995
62-63	21.68149871172763	28.266470361891916	27.95170888279997	22.10032204358048
64-65	21.697939153236373	28.044165331213243	28.083257424913054	22.174638090637327
66-67	21.559530568571052	28.123334934707607	27.955190636327902	22.36194386039344
68-69	21.90505841486767	28.189518801533488	27.875883603086226	22.029539180512618
70-71	21.85573819295488	28.036291179761935	27.97015488105048	22.137815746232707
72-73	21.700544261439436	28.130207401185498	28.063074930960152	22.10617340641491
74-75	21.817435395016993	28.113765164576748	27.979874091408867	22.088925348997396
76-77	21.8466614792651	28.11138474208721	27.851524445407055	22.190429333240637
78-79	21.86845639306693	28.158973667409338	27.940950366252586	22.03161957327115
80-81	21.827346423162137	28.073348343577297	27.977996067364806	22.12130916589576
82-83	21.727856696635918	28.097979511054348	28.086009399078097	22.088154393231637
84-85	21.781406396926766	27.938000525872482	28.002727406423666	22.27786567077709
86-87	21.937873966557447	28.089823546121238	27.906159846601682	22.06614264071963
88-89	21.793710565805927	27.954571951037334	27.98960079947776	22.262116683678983
90-91	21.8005196945295	27.863340286540595	28.01378662076712	22.32235339816279
92-93	21.926400299479255	28.016605055049364	27.82786182062886	22.22913282484252
94-95	21.815669110513234	27.787698647023195	27.995099531300205	22.401532711163362
96-97	21.915172935857306	27.745605769114256	27.964987643620194	22.374233651408243
98-99	21.937964700741812	27.792534707990978	27.906021302726675	22.363479288540535
100	22.020711815863926	27.679876742705744	27.89065156291988	22.40875987851045
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1673.0
1	1309.0
2	1223.5
3	1538.0
4	1422.0
5	1181.0
6	1011.0
7	888.5
8	853.5
9	846.5
10	760.0
11	720.5
12	755.5
13	807.0
14	905.0
15	1073.5
16	1332.0
17	1732.5
18	2398.0
19	3433.0
20	4993.5
21	7287.5
22	10459.5
23	14687.5
24	20890.5
25	29001.0
26	39135.0
27	51408.5
28	66040.5
29	83487.5
30	100510.5
31	114537.0
32	126629.0
33	139087.5
34	151715.5
35	161760.5
36	170931.0
37	180794.0
38	189581.0
39	195383.0
40	198547.0
41	200607.5
42	199730.5
43	197877.0
44	195214.5
45	191194.0
46	187852.0
47	182933.0
48	177921.0
49	174286.0
50	170460.0
51	164578.0
52	155904.0
53	147871.0
54	141325.0
55	134705.0
56	125183.0
57	113799.5
58	103737.5
59	94816.0
60	84344.0
61	72218.5
62	61819.0
63	52145.5
64	42990.0
65	35153.0
66	28448.5
67	22333.0
68	17167.0
69	13303.0
70	10116.5
71	7706.0
72	5967.5
73	4537.0
74	3335.0
75	2434.0
76	1667.0
77	1155.5
78	867.5
79	603.5
80	385.0
81	257.5
82	185.0
83	117.5
84	82.0
85	77.0
86	60.0
87	42.0
88	40.5
89	29.0
90	20.5
91	21.0
92	19.0
93	18.0
94	16.0
95	14.5
96	13.5
97	11.0
98	13.0
99	14.5
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.018194207933601297
2	0.04029099327901326
3	0.04203735212082807
4	0.044942010194458835
5	0.04857728778354274
6	0.04504893012354954
7	0.042910531541735485
8	0.04251849180173624
9	0.04275015164809943
10-11	0.04341840120491632
12-13	0.0443272206021873
14-15	0.04284816158309924
16-17	0.03905250410037928
18-19	0.04215318204400967
20-21	0.04134237258173851
22-23	0.04257195176628159
24-25	0.03931089392901514
26-27	0.03948018381674209
28-29	0.039979143485832036
30-31	0.04006824342674096
32-33	0.03985440356855955
34-35	0.03972075365719617
36-37	0.03695865548901968
38-39	0.037511075122654974
40-41	0.03866046436038004
42-43	0.03853572444310755
44-45	0.03719031533538287
46-47	0.03618348600311208
48-49	0.03637059587902081
50-51	0.037662545022200136
52-53	0.03831297459083525
54-55	0.037217045317655545
56-57	0.035631066369476785
58-59	0.036602255725384
60-61	0.035960736150839785
62-63	0.03620130599129386
64-65	0.03289569818357296
66-67	0.03405399741538891
68-69	0.03517665667084129
70-71	0.03650424579038419
72-73	0.03335901787629934
74-75	0.03371541763993502
76-77	0.03441039717902459
78-79	0.03482916690129651
80-81	0.03477570693675116
82-83	0.03333228789402667
84-85	0.034766796942660265
86-87	0.03504300675947791
88-89	0.03588945619811265
90-91	0.036254765955839215
92-93	0.03454404709038797
94-95	0.035791446263112835
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5611676.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.84571613155855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.70107333387078	48.20333881726299
2	14.042885943792962	17.650704473829325
3	4.283646904821089	8.076265721646468
4	1.7935438233263719	4.508661839611175
5	0.9091960120279291	2.8569537239926164
6	0.5481497599233355	2.066931852583435
7	0.34700045128675533	1.5265244301363097
8	0.23439634086886044	1.1784644720416353
9	0.17686506689005024	1.000369060862509
>10	0.9096998114934217	9.93934896269617
>50	0.04115437799426816	1.748439432030317
>100	0.012246155201082992	1.150871351726063
>500	1.136149718656323E-4	0.05394422783216099
>1k	2.84035312474216E-5	0.03918163374890938
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.7819988181783837E-5	0.0
3	0.0	0.0	0.0	3.5639976363567675E-5	0.0
4	0.0	0.0	0.0	5.345996454535151E-5	0.0
5	0.0	0.0	0.0	5.345996454535151E-5	0.0
6	0.0	0.0	0.0	5.345996454535151E-5	0.0
7	0.0	0.0	0.0	5.345996454535151E-5	0.0
8	0.0	0.0	0.0	8.909994090891919E-5	0.0
9	0.0	0.0	0.0	1.2473991727248688E-4	0.0
10-11	0.0	0.0	0.0	2.0492986409051414E-4	0.0
12-13	0.0	0.0	0.0	2.4947983454497375E-4	0.0
14-15	0.0	0.0	0.0	2.7620981681764946E-4	0.0
16-17	0.0	0.0	0.0	2.9402980499943334E-4	0.0
18-19	0.0	0.0	0.0	3.2075978727210905E-4	0.0
20-21	0.0	0.0	0.0	4.009497340901364E-4	0.0
22-23	0.0	0.0	0.0	5.880596099988667E-4	0.0
24-25	0.0	0.0	0.0	0.001087019279088814	0.0
26-27	0.0	0.0	0.0	0.0018354587827237353	0.0
28-29	0.0	3.5639976363567675E-5	0.0	0.005648936253625477	0.0
30-31	0.0	3.5639976363567675E-5	0.0	0.01793581810496543	0.0
32-33	0.0	3.5639976363567675E-5	0.0	0.04525385998764006	0.0
34-35	0.0	3.5639976363567675E-5	0.0	0.08972364049528161	0.0
36-37	0.0	4.4549970454459593E-5	0.0	0.1586691747706033	0.0
38-39	0.0	5.345996454535151E-5	0.0	0.247724565709068	0.0
40-41	0.0	5.345996454535151E-5	0.0	0.3412705936693423	0.0
42-43	0.0	5.345996454535151E-5	0.0	0.4324376532073484	0.0
44-45	0.0	5.345996454535151E-5	0.0	0.5445699288412231	0.0
46-47	0.0	5.345996454535151E-5	0.0	0.6770348109905133	0.0
48-49	0.0	5.345996454535151E-5	0.0	0.8125201811366158	0.0
50-51	0.0	7.127995272713535E-5	0.0	0.9441297038531804	0.0
52-53	0.0	7.127995272713535E-5	0.0	1.0727811085315688	0.0
54-55	0.0	7.127995272713535E-5	0.0	1.2150915341512945	8.909994090891919E-6
56-57	0.0	7.127995272713535E-5	0.0	1.379106705376433	1.7819988181783837E-5
58-59	0.0	7.127995272713535E-5	0.0	1.5542415492270045	1.7819988181783837E-5
60-61	0.0	7.127995272713535E-5	0.0	1.7300624626225747	1.7819988181783837E-5
62-63	0.0	7.127995272713535E-5	0.0	1.90003841989452	1.7819988181783837E-5
64-65	0.0	7.127995272713535E-5	0.0	2.084279277705983	1.7819988181783837E-5
66-67	0.0	7.127995272713535E-5	0.0	2.294439308327851	1.7819988181783837E-5
68-69	0.0	7.127995272713535E-5	0.0	2.521314487864232	1.7819988181783837E-5
70-71	8.909994090891919E-6	7.127995272713535E-5	0.0	2.7602983493701347	1.7819988181783837E-5
72-73	1.7819988181783837E-5	7.127995272713535E-5	0.0	3.0041559776437556	1.7819988181783837E-5
74-75	1.7819988181783837E-5	7.127995272713535E-5	0.0	3.2486016655273753	1.7819988181783837E-5
76-77	1.7819988181783837E-5	7.127995272713535E-5	0.0	3.5089338728750556	1.7819988181783837E-5
78-79	1.7819988181783837E-5	7.127995272713535E-5	0.0	3.7966554020581373	1.7819988181783837E-5
80-81	1.7819988181783837E-5	7.127995272713535E-5	0.0	4.1054490672661785	1.7819988181783837E-5
82-83	1.7819988181783837E-5	7.127995272713535E-5	0.0	4.418376969732394	1.7819988181783837E-5
84-85	1.7819988181783837E-5	8.909994090891919E-5	0.0	4.731206862263609	1.7819988181783837E-5
86-87	1.7819988181783837E-5	8.909994090891919E-5	0.0	5.061108303472974	1.7819988181783837E-5
88	1.7819988181783837E-5	8.909994090891919E-5	0.0	5.324772848610647	1.7819988181783837E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4910	0.0	41.170364	1
GTATCAA	8630	0.0	36.170547	1
TCAACGC	8835	0.0	33.361027	4
ATCAACG	9045	0.0	32.534504	3
CAACGCA	9370	0.0	31.606716	5
AACGCAG	9990	0.0	29.641438	6
TATCAAC	10495	0.0	28.756168	2
ACGCAGA	11725	0.0	25.014736	7
GTACATG	14230	0.0	24.645163	1
CGCAGAG	11795	0.0	24.388083	8
TACATGG	14185	0.0	23.330374	2
ACATGGG	14770	0.0	21.483335	3
GCAGAGT	14100	0.0	19.501183	9
GAGTACA	10725	0.0	18.71666	1
GTGGTAT	3035	0.0	18.587471	1
CATGGGG	10870	0.0	18.076948	4
GTCTACG	650	0.0	17.35784	1
AGAGTAC	11710	0.0	17.219662	10-11
CAGAGTA	12925	0.0	16.9283	10-11
AGTACAT	11140	0.0	16.077488	2
>>END_MODULE