FastQCFastQC Report
Wed 25 May 2016
SRR2049344_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049344_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5611676
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122450.21820575528594308No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121130.21585351684594764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68710.12244113879703675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90650.058.3968961
GTATCAA156400.037.8149071
TCAACGC187550.031.1218224
ATCAACG188900.030.8745293
CAACGCA194100.030.095815
TATCAAC197500.029.6728922
AACGCAG200900.029.1454776
GTGGTAT38850.027.3486941
TGGTATC37500.026.4430582
ACGCAGA235000.024.6563327
GTACATG143500.024.0143721
CGCAGAG240600.023.9065278
TACATGG144700.022.8646532
GCAGAGT263950.021.4709389
ACATGGG145250.021.4515113
GAGTACT190000.020.83451712-13
CAGAGTA258200.020.3643810-11
AGAGTAC242300.018.97356610-11
GTACTTT210950.018.62461314-15
GAGTACA108150.017.7358821
Produced by FastQC (version 0.11.5)