##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049344_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5611676
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.803592367057544	31.0	31.0	34.0	30.0	34.0
2	31.919344773290547	33.0	31.0	34.0	30.0	34.0
3	31.969351758725914	33.0	31.0	34.0	30.0	34.0
4	35.71996173692138	37.0	35.0	37.0	33.0	37.0
5	35.764656049280106	37.0	35.0	37.0	35.0	37.0
6	35.84798890741376	37.0	35.0	37.0	35.0	37.0
7	35.79658287470624	37.0	35.0	37.0	35.0	37.0
8	35.809937352049545	37.0	35.0	37.0	35.0	37.0
9	37.54677818177671	39.0	37.0	39.0	35.0	39.0
10-11	37.505599218486594	39.0	37.0	39.0	35.0	39.0
12-13	37.45105339296139	39.0	37.0	39.0	34.5	39.0
14-15	38.75297460152724	40.0	38.0	41.0	35.0	41.0
16-17	38.77743057510804	40.0	38.0	41.0	35.0	41.0
18-19	38.84158841315856	40.0	38.0	41.0	35.0	41.0
20-21	38.87256115641744	40.0	38.0	41.0	35.0	41.0
22-23	38.84085966474187	40.0	38.0	41.0	35.0	41.0
24-25	38.818950256572194	40.0	38.0	41.0	35.0	41.0
26-27	38.708262380080384	40.0	38.0	41.0	34.5	41.0
28-29	38.62662455922259	40.0	38.0	41.0	34.0	41.0
30-31	38.53148373141999	40.0	38.0	41.0	34.0	41.0
32-33	38.39991724397488	40.0	38.0	41.0	34.0	41.0
34-35	38.29817589254975	40.0	38.0	41.0	34.0	41.0
36-37	38.18481020643387	40.0	38.0	41.0	34.0	41.0
38-39	38.08907089432818	40.0	38.0	41.0	33.5	41.0
40-41	37.978257208719825	40.0	38.0	41.0	33.0	41.0
42-43	37.83861033673362	40.0	37.0	41.0	33.0	41.0
44-45	37.66604834277674	40.0	37.0	41.0	33.0	41.0
46-47	37.44424847407441	40.0	37.0	41.0	33.0	41.0
48-49	37.24006268359042	40.0	37.0	41.0	32.0	41.0
50-51	37.094199932426605	40.0	37.0	41.0	31.5	41.0
52-53	36.95299701907238	40.0	36.0	41.0	31.0	41.0
54-55	36.80790961559434	40.0	36.0	41.0	31.0	41.0
56-57	36.66365547476369	39.0	35.5	41.0	31.0	41.0
58-59	36.46948647783657	39.0	35.0	41.0	31.0	41.0
60-61	36.28923302058066	39.0	35.0	41.0	31.0	41.0
62-63	36.081375332431875	39.0	35.0	41.0	30.0	41.0
64-65	35.85092181016866	38.0	35.0	40.0	30.0	41.0
66-67	35.581835088126965	37.5	35.0	40.0	30.0	41.0
68-69	35.29100789140357	37.0	35.0	40.0	29.0	41.0
70-71	34.96032459821272	36.5	34.0	39.5	29.0	41.0
72-73	34.66337115685225	36.0	34.0	39.0	29.0	41.0
74-75	34.359644159784	36.0	34.0	39.0	29.0	40.5
76-77	34.03567659643928	35.0	34.0	38.5	29.0	40.0
78-79	33.70405686286949	35.0	34.0	37.0	29.0	39.0
80-81	33.39129308249443	35.0	34.0	37.0	28.0	39.0
82-83	33.0962463798694	35.0	34.0	36.0	28.0	39.0
84-85	32.82997673065943	35.0	33.5	36.0	28.0	37.0
86-87	32.60316276278246	35.0	33.0	36.0	27.0	37.0
88-89	32.37829170821694	35.0	33.0	35.0	27.0	37.0
90-91	32.187246733417965	35.0	33.0	35.0	27.0	36.0
92-93	32.0096400790067	35.0	33.0	35.0	26.0	36.0
94-95	31.855584944676064	35.0	33.0	35.0	26.0	36.0
96-97	31.657050674343992	35.0	33.0	35.0	25.5	36.0
98-99	31.510321782654593	35.0	33.0	35.0	25.0	35.0
100	31.397287013719254	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19644065237498864
1101	2	0.20815402629623492
1101	3	0.4037305544249712
1101	4	0.2405585104732637
1101	5	0.20908691217576347
1101	6	0.22021362354686858
1101	7	0.13273342762817464
1101	8	0.1196219086287229
1101	9	0.2541228220842626
1101	10-11	0.28276254807823165
1101	12-13	0.23135658584616436
1101	14-15	0.3565736680549989
1101	16-17	0.2586291868776911
1101	18-19	0.3611283873936344
1101	20-21	0.31730542671472506
1101	22-23	0.31367779131468865
1101	24-25	0.33717721515836985
1101	26-27	0.40913866492008566
1101	28-29	0.37132969338318844
1101	30-31	0.32244066130680693
1101	32-33	0.4027652769494594
1101	34-35	0.43783572245428104
1101	36-37	0.44501262727369806
1101	38-39	0.41365259541589694
1101	40-41	0.45605430320214424
1101	42-43	0.4490089409622655
1101	44-45	0.4266304930946134
1101	46-47	0.44864321470520707
1101	48-49	0.5321460460838239
1101	50-51	0.4470755690866923
1101	52-53	0.5306607037098416
1101	54-55	0.4900668033410298
1101	56-57	0.49357486640305837
1101	58-59	0.5360807343761422
1101	60-61	0.503787439820897
1101	62-63	0.4336081491162105
1101	64-65	0.349682141245232
1101	66-67	0.3563447670586868
1101	68-69	0.3939367275610479
1101	70-71	0.4276358558285125
1101	72-73	0.3567118545724739
1101	74-75	0.4561363846373325
1101	76-77	0.4404821363706617
1101	78-79	0.44138878644896096
1101	80-81	0.48640225275694604
1101	82-83	0.42947364827470835
1101	84-85	0.39150389376852957
1101	86-87	0.34402287117843144
1101	88-89	0.48404140733020995
1101	90-91	0.4621275341607358
1101	92-93	0.4403823503446347
1101	94-95	0.4281503022803257
1101	96-97	0.5202704024852913
1101	98-99	0.5830633485962231
1101	100	0.6797945190100947
1102	1	0.21304368814298158
1102	2	0.1720297009632148
1102	3	0.3598693865963405
1102	4	0.20005299678867772
1102	5	0.18921109672483993
1102	6	0.2082116765433497
1102	7	0.20559934900080634
1102	8	0.23413474652177513
1102	9	0.20278980806427427
1102	10-11	0.26501551861280603
1102	12-13	0.214068718309683
1102	14-15	0.29909938496378885
1102	16-17	0.2730558078498433
1102	18-19	0.2904919065657481
1102	20-21	0.24053184040469944
1102	22-23	0.25835370813535263
1102	24-25	0.27126481038452965
1102	26-27	0.3460649173773547
1102	28-29	0.2714390078766371
1102	30-31	0.2856356512393674
1102	32-33	0.23568187060845958
1102	34-35	0.2552459928879216
1102	36-37	0.2526714161318182
1102	38-39	0.24703268014319235
1102	40-41	0.24820174438445264
1102	42-43	0.3093938989595131
1102	44-45	0.2873092779742237
1102	46-47	0.31500194629618505
1102	48-49	0.25982304182865334
1102	50-51	0.3024656687439773
1102	52-53	0.3621296513809966
1102	54-55	0.27653291546729264
1102	56-57	0.29228513384354926
1102	58-59	0.27448357431804027
1102	60-61	0.3063307752507569
1102	62-63	0.36873549931739547
1102	64-65	0.30119571431388437
1102	66-67	0.23011887564668143
1102	68-69	0.28277652251976804
1102	70-71	0.30057661750804243
1102	72-73	0.319129884015787
1102	74-75	0.22349715223164424
1102	76-77	0.28451845140990173
1102	78-79	0.30148204488470753
1102	80-81	0.2394128918776346
1102	82-83	0.3292044983143825
1102	84-85	0.2827498624541036
1102	86-87	0.251360482595409
1102	88-89	0.2660358330542323
1102	90-91	0.2908852403598772
1102	92-93	0.33456866550969266
1102	94-95	0.3196910911498314
1102	96-97	0.3534224334492855
1102	98-99	0.34817884904076024
1102	100	0.44698284117407994
1103	1	0.09063542832856797
1103	2	0.1878618861920529
1103	3	0.323825494002282
1103	4	0.18067515000742418
1103	5	0.1203411717537648
1103	6	0.1309421531760222
1103	7	0.1185020733012152
1103	8	0.14613876902126322
1103	9	0.21321034912827486
1103	10-11	0.19747616899016407
1103	12-13	0.20588827182326241
1103	14-15	0.25224709186222327
1103	16-17	0.23811998893441455
1103	18-19	0.22571275125262957
1103	20-21	0.2595062747197261
1103	22-23	0.22544606198429307
1103	24-25	0.2548033929358908
1103	26-27	0.2832668402124412
1103	28-29	0.3516904749131271
1103	30-31	0.25019911843703113
1103	32-33	0.24419631904981287
1103	34-35	0.2732233628280625
1103	36-37	0.2958710691758739
1103	38-39	0.28535370473198185
1103	40-41	0.3264765679431747
1103	42-43	0.37278392543639427
1103	44-45	0.38351380668500923
1103	46-47	0.3382934372469535
1103	48-49	0.294924812446844
1103	50-51	0.32032560702729995
1103	52-53	0.3169554613276233
1103	54-55	0.2808021014195532
1103	56-57	0.27824714499953274
1103	58-59	0.31339946243079453
1103	60-61	0.27712059100477404
1103	62-63	0.3285501155223187
1103	64-65	0.2626821220806974
1103	66-67	0.3065581732759739
1103	68-69	0.27549763661287585
1103	70-71	0.25975121574116145
1103	72-73	0.3019004608390716
1103	74-75	0.2873190918513302
1103	76-77	0.25060689882101883
1103	78-79	0.2359798799102819
1103	80-81	0.23924802508531684
1103	82-83	0.2663213546677383
1103	84-85	0.3156756036443298
1103	86-87	0.24008133390262287
1103	88-89	0.2661372207069519
1103	90-91	0.23949009462615578
1103	92-93	0.31202829686313294
1103	94-95	0.2950723191772333
1103	96-97	0.317937760715612
1103	98-99	0.31182301697716497
1103	100	0.3812032162927146
1104	1	0.12048896327002012
1104	2	0.13371665756761075
1104	3	0.25733994328700405
1104	4	0.1928782984002808
1104	5	0.14499821940403024
1104	6	0.16358551025152934
1104	7	0.21200329887246738
1104	8	0.15981908442059023
1104	9	0.15797388653680855
1104	10-11	0.15132059917073803
1104	12-13	0.17005663711832852
1104	14-15	0.25689144445789935
1104	16-17	0.23248189648042228
1104	18-19	0.25465045713387724
1104	20-21	0.27596017832227915
1104	22-23	0.24373575762645316
1104	24-25	0.2794448704963699
1104	26-27	0.30293759678362164
1104	28-29	0.34195802216292037
1104	30-31	0.337410329481024
1104	32-33	0.39569157270708644
1104	34-35	0.33676212317855203
1104	36-37	0.3268750104604692
1104	38-39	0.30802770833072657
1104	40-41	0.29709116163142113
1104	42-43	0.30776579533417703
1104	44-45	0.2914754755379292
1104	46-47	0.33625384600888
1104	48-49	0.31743167897713676
1104	50-51	0.2545304208162733
1104	52-53	0.19243946179302895
1104	54-55	0.19004748796574233
1104	56-57	0.20330136270752774
1104	58-59	0.2746452373843411
1104	60-61	0.2526352669610361
1104	62-63	0.2807344603160473
1104	64-65	0.210845973980895
1104	66-67	0.22861695917589486
1104	68-69	0.18839792068856553
1104	70-71	0.26927292913660494
1104	72-73	0.23438263731070919
1104	74-75	0.24418433067236833
1104	76-77	0.13891235525638734
1104	78-79	0.16991686401527062
1104	80-81	0.22898576683811456
1104	82-83	0.28102615783524953
1104	84-85	0.26430501933386097
1104	86-87	0.2408541655684715
1104	88-89	0.2933948052718591
1104	90-91	0.2600262585132995
1104	92-93	0.31925867239439043
1104	94-95	0.34790646175290973
1104	96-97	0.3819480292501787
1104	98-99	0.35702217290932126
1104	100	0.3447663697838692
1105	1	0.17991748261369622
1105	2	0.19137792675461895
1105	3	0.31783041304307247
1105	4	0.20528743394539362
1105	5	0.15771709773390796
1105	6	0.15335599676224376
1105	7	0.12102015370410868
1105	8	0.13658860749323765
1105	9	0.16979786218227844
1105	10-11	0.19288286825523215
1105	12-13	0.1561147038594939
1105	14-15	0.21880444651140607
1105	16-17	0.20361960341435292
1105	18-19	0.21718250988779175
1105	20-21	0.2617291150753758
1105	22-23	0.24071924442799997
1105	24-25	0.24673065164424912
1105	26-27	0.27950191961004833
1105	28-29	0.2627256206787507
1105	30-31	0.2607826201303922
1105	32-33	0.33430553346727976
1105	34-35	0.36598851767048757
1105	36-37	0.3647400801949203
1105	38-39	0.3833404604098689
1105	40-41	0.393178858615542
1105	42-43	0.3673707845702978
1105	44-45	0.4168161520133893
1105	46-47	0.5285597876895665
1105	48-49	0.4664397598892336
1105	50-51	0.4090794012279133
1105	52-53	0.4577178638496022
1105	54-55	0.45426538917367765
1105	56-57	0.3774661008745639
1105	58-59	0.3753949923500812
1105	60-61	0.3908429092003445
1105	62-63	0.43404841240204917
1105	64-65	0.42388928787384117
1105	66-67	0.48373270162390014
1105	68-69	0.3921979752849367
1105	70-71	0.41637118080035407
1105	72-73	0.5013643927533025
1105	74-75	0.4480797375730248
1105	76-77	0.3559380556740024
1105	78-79	0.4279993706334011
1105	80-81	0.42603859497179997
1105	82-83	0.36643457068585406
1105	84-85	0.3652698315794112
1105	86-87	0.34573335080694534
1105	88-89	0.3669348568345754
1105	90-91	0.4383586664451258
1105	92-93	0.5035085474999761
1105	94-95	0.49185713066280456
1105	96-97	0.5776953222768206
1105	98-99	0.5541357957472108
1105	100	0.5961257706803238
1106	1	0.11014114638974704
1106	2	0.11810376680868728
1106	3	0.2648189456996768
1106	4	0.1760095966256614
1106	5	0.12221205705661475
1106	6	0.14116818452406932
1106	7	0.17148355590333608
1106	8	0.1254727184624116
1106	9	0.13586558608551513
1106	10-11	0.1505671494819083
1106	12-13	0.14648251853760286
1106	14-15	0.2216491011619155
1106	16-17	0.18952563646500664
1106	18-19	0.21547796042321465
1106	20-21	0.22500310026035208
1106	22-23	0.22639266896639043
1106	24-25	0.2180365411220606
1106	26-27	0.21722782755202985
1106	28-29	0.2510702832271079
1106	30-31	0.2732225334489513
1106	32-33	0.19253133840111758
1106	34-35	0.19681411531094284
1106	36-37	0.21060171556088392
1106	38-39	0.2005130264350612
1106	40-41	0.20578684492235055
1106	42-43	0.2319975035711508
1106	44-45	0.1697986829754896
1106	46-47	0.2130244293472714
1106	48-49	0.18777707273117272
1106	50-51	0.2140782283130349
1106	52-53	0.2596064080337115
1106	54-55	0.21912880844570992
1106	56-57	0.2384717392679363
1106	58-59	0.2664088092200956
1106	60-61	0.22764953250612763
1106	62-63	0.27218968848638525
1106	64-65	0.2219124642012602
1106	66-67	0.1782503832316138
1106	68-69	0.21170070138780517
1106	70-71	0.26430390152931693
1106	72-73	0.31904397559890185
1106	74-75	0.2897105614398612
1106	76-77	0.21173472533512694
1106	78-79	0.26592942121661167
1106	80-81	0.24009781137728936
1106	82-83	0.2646452621578206
1106	84-85	0.2399344669860426
1106	86-87	0.2592746110283599
1106	88-89	0.3073878338706493
1106	90-91	0.2579099139190717
1106	92-93	0.28996784180851876
1106	94-95	0.2531253053820812
1106	96-97	0.32547413573731276
1106	98-99	0.31636813842117917
1106	100	0.3264863361395598
1107	1	0.07983053946771435
1107	2	0.021272850593678783
1107	3	0.13371146941979006
1107	4	0.09952891013124088
1107	5	0.07462923116072773
1107	6	0.12909070134303136
1107	7	0.07642758575154573
1107	8	0.10480729347571582
1107	9	0.09761539978862288
1107	10-11	0.1158007157315879
1107	12-13	0.10894193653487605
1107	14-15	0.15885847780749174
1107	16-17	0.09181072323662676
1107	18-19	0.11692883683012667
1107	20-21	0.07242685784776626
1107	22-23	0.07920458133393993
1107	24-25	0.10092986088437073
1107	26-27	0.16872432618811928
1107	28-29	0.12609117674963954
1107	30-31	0.15867645422202514
1107	32-33	0.15508505784810467
1107	34-35	0.08124693702065144
1107	36-37	0.1663602955234964
1107	38-39	0.15136640047603578
1107	40-41	0.13547308414297987
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2216	70-71	-0.7345343740654755
2216	72-73	-0.7975204614280145
2216	74-75	-0.8102896701844031
2216	76-77	-0.7227487377090256
2216	78-79	-0.9308924094470257
2216	80-81	-1.0596362582221985
2216	82-83	-0.8967191143673574
2216	84-85	-1.0063385207731912
2216	86-87	-0.7821369778766467
2216	88-89	-1.145958196884692
2216	90-91	-0.966058171148461
2216	92-93	-1.0763442656971094
2216	94-95	-1.044109912105629
2216	96-97	-1.1045873503350911
2216	98-99	-1.2439299465755163
2216	100	-1.1864074150125852
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	394.0
3	18.0
4	39.0
5	89.0
6	255.0
7	670.0
8	1638.0
9	2732.0
10	4230.0
11	6052.0
12	8566.0
13	11814.0
14	14654.0
15	18405.0
16	22928.0
17	30918.0
18	30752.0
19	17927.0
20	13565.0
21	13343.0
22	14476.0
23	15925.0
24	18820.0
25	23486.0
26	30221.0
27	38778.0
28	51838.0
29	67108.0
30	87871.0
31	113335.0
32	146656.0
33	191201.0
34	262134.0
35	382129.0
36	625991.0
37	1133703.0
38	1574562.0
39	631789.0
40	2664.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.58421930928727	20.449987066673394	14.245199115292717	23.720594508746622
2	15.60280743221811	20.414061681394294	39.48611787280662	24.497013013580972
3	17.9193167959091	24.9148917364438	30.945799436745812	26.219992030901285
4	12.721284237109995	16.95501199820232	37.87561429976154	32.44808946492615
5	12.716521766402764	37.67441313432921	35.712806655266625	13.8962584440014
6	31.196918710203512	37.93319500270507	16.94509804201098	13.924788245080435
7	26.65697378109499	32.82776838862401	22.290096577207947	18.225161253073058
8	24.960840282883737	36.40572425072626	20.114480024891147	18.518955441498857
9	24.686867167669693	16.372809121552994	21.5289692419876	37.41135446878972
10-11	23.91548442925073	26.85336787084643	28.63573199878254	20.595415701120306
12-13	24.48838095727092	24.35460855594266	29.066994773186828	22.09001571359959
14-15	21.741695741498653	26.410868139229166	26.612448096992182	25.234988022280003
16-17	20.823363790668562	29.737850107142698	27.39656163266761	22.04222446952113
18-19	20.69660834807715	28.123329654396446	30.230587994619434	20.949474002906975
20-21	21.716118221837867	27.350144667260846	30.06413152732328	20.86960558357801
22-23	21.37773991228289	27.216093730286637	30.126864059863756	21.279302297566716
24-25	21.31248048709903	27.326873345412917	30.074619471701126	21.286026695786926
26-27	21.075729109661236	27.268436703279043	30.188198777797638	21.467635409262087
28-29	21.063272493603268	27.282416519970532	30.340969729704586	21.31334125672161
30-31	21.36881400216388	27.620173333258492	29.88372189375526	21.12729077082237
32-33	21.12430742517198	27.411626988624416	30.209983912091737	21.25408167411187
34-35	21.249005087518885	27.155513029132393	30.208095640614935	21.387386242733786
36-37	21.513744653332438	27.49119558463225	29.66560952500262	21.3294502370327
38-39	21.404705934974483	27.63429029047295	29.737429674311077	21.22357410024149
40-41	21.29509765320285	27.395973693746576	29.85933152335504	21.449597129695537
42-43	21.312419581369156	27.388724582901236	29.724392137393448	21.574463698336167
44-45	21.404427191483308	27.50014411952681	29.56883625286379	21.52659243612609
46-47	21.494303701544844	27.722627335300604	29.15370743880989	21.629361524344667
48-49	21.485417616330643	27.729677309674866	28.95768861618559	21.827216457808902
50-51	21.587518115207597	27.725540738668336	28.75349106167925	21.93345008444482
52-53	21.519278215211404	27.761146686934048	28.823892102939514	21.895682994915038
54-55	21.600361818329564	28.079670243507444	28.58135815880375	21.738609779359248
56-57	21.484683022762116	27.848525451253277	28.614476778572573	22.052314747412037
58-59	21.37364825209325	27.472385891578256	28.99292269439603	22.16104316193247
60-61	21.5493313393919	27.824927570528967	28.63514150699632	21.990599583082815
62-63	21.412535856331253	27.827756315409907	28.667476532223873	22.092231296034967
64-65	21.378258408460464	27.623315013424293	28.814835268279616	22.18359130983563
66-67	21.325733484710163	27.696773987753588	28.63650546911462	22.34098705842163
68-69	21.659569152338136	27.759802081014843	28.48939043825198	22.09123832839504
70-71	21.634024623936597	27.62113697861067	28.511035116476812	22.23380328097592
72-73	21.44283456089021	27.719880558565922	28.586056380683615	22.251228499860247
74-75	21.51544981387731	27.72529541820447	28.457035854702234	22.302218913215984
76-77	21.58077430538973	27.7062339752614	28.340407325065275	22.372584394283592
78-79	21.618297838405446	27.72652324942651	28.35497571274614	22.3002031994219
80-81	21.51690121694322	27.6321259894157	28.41268112965491	22.438291663986167
82-83	21.392484882008432	27.64288527968407	28.504117847850573	22.460511990456926
84-85	21.44333571237001	27.545983571281553	28.36421180376422	22.646468912584215
86-87	21.509724196928342	27.669711344966213	28.30239248399007	22.51817197411538
88-89	21.36113353751284	27.528628151983614	28.36401000754602	22.746228302957526
90-91	21.25692704595672	27.410038173264674	28.423275409029173	22.909759371749434
92-93	21.297729852642682	27.54670294910657	28.292577960021596	22.862989238229144
94-95	21.194967483099113	27.327588964489053	28.453952277893848	23.02349127451799
96-97	21.246077472731432	27.312569177173224	28.40171704627125	23.039636303824096
98-99	21.188424265855502	27.390920935872128	28.342820819584446	23.077833978687927
100	21.25717776442053	27.27966582085932	28.31605454486162	23.14710186985853
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	714.0
1	533.0
2	621.5
3	902.0
4	590.0
5	392.0
6	977.0
7	2413.5
8	3989.5
9	4610.0
10	3637.0
11	2422.5
12	2348.5
13	2718.0
14	3138.5
15	3674.5
16	4292.0
17	4937.5
18	5806.0
19	6987.5
20	8644.0
21	10712.5
22	13545.0
23	17866.5
24	23632.0
25	31116.5
26	41127.0
27	52908.0
28	66989.5
29	83678.5
30	100415.5
31	113752.0
32	124861.5
33	137186.5
34	149071.5
35	158692.0
36	167763.0
37	177912.5
38	187222.0
39	192914.5
40	196241.0
41	198465.5
42	197919.5
43	196911.5
44	195065.5
45	191318.0
46	187918.5
47	183554.0
48	177950.5
49	174299.5
50	170861.0
51	164556.0
52	155105.0
53	146947.5
54	140510.5
55	132928.5
56	124009.5
57	113262.0
58	103195.5
59	94192.0
60	83437.0
61	71160.0
62	60814.0
63	51117.0
64	41563.0
65	33786.5
66	27137.5
67	20807.5
68	15917.0
69	12340.0
70	9141.0
71	6737.5
72	5093.0
73	3794.0
74	2764.5
75	1945.0
76	1301.5
77	871.0
78	577.5
79	353.5
80	197.0
81	103.0
82	57.0
83	35.0
84	14.0
85	9.5
86	9.5
87	7.5
88	5.0
89	2.5
90	1.0
91	0.0
92	0.0
93	0.5
94	0.5
95	1.0
96	1.0
97	0.0
98	0.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038081314744472065
2	0.0
3	0.0
4	3.5639976363567675E-5
5	0.0
6	0.0
7	0.0
8	7.484395036349213E-4
9	0.0
10-11	0.0
12-13	0.044942010194458835
14-15	1.2473991727248688E-4
16-17	1.7819988181783837E-5
18-19	8.909994090891919E-6
20-21	9.80099349998111E-5
22-23	0.0
24-25	7.127995272713535E-5
26-27	5.345996454535151E-5
28-29	3.4748976954478486E-4
30-31	8.909994090891919E-6
32-33	1.425599054542707E-4
34-35	2.6729982272675756E-5
36-37	5.345996454535151E-5
38-39	3.1184979318121716E-4
40-41	1.1582992318159495E-4
42-43	4.544096986354879E-4
44-45	2.5838982863586564E-4
46-47	9.80099349998111E-5
48-49	7.484395036349213E-4
50-51	4.187697222719202E-4
52-53	5.52419633635299E-4
54-55	3.5639976363567675E-4
56-57	4.811396809081636E-4
58-59	1.1582992318159495E-4
60-61	1.7819988181783837E-4
62-63	8.018994681802728E-4
64-65	3.831297459083525E-4
66-67	0.00130085913727022
68-69	0.001550338971815194
70-71	4.009497340901364E-4
72-73	9.89009344089003E-4
74-75	9.800993499981111E-4
76-77	7.127995272713535E-4
78-79	0.003448167713175173
80-81	0.0027977381445400628
82-83	0.0012028492022704092
84-85	0.002869018097267198
86-87	0.0014523290368153828
88-89	0.001969108694087114
90-91	0.0027977381445400628
92-93	0.00260171827454044
94-95	0.0037332875240837144
96-97	0.003189777884539307
98-99	0.0028779280913580896
100	0.0013364991136337878
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5611676.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.9169433171201
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.79227749944889	46.17033872309365
2	14.581748529751662	17.765510973027627
3	4.394555772131619	8.031087146245943
4	1.8697424158946996	4.5559597106669045
5	0.9544966087213391	2.907250790493059
6	0.5772957344607281	2.1100254920015638
7	0.3608170371062668	1.5385909698077571
8	0.25132819514878874	1.224811633429829
9	0.20113315534157275	1.102717532082249
>10	0.9543297995817199	10.208000571643
>50	0.046382073401214224	1.9258995132950745
>100	0.015131053252790697	1.4694303028366493
>500	4.396901022616772E-4	0.19936586052444258
>1k	2.3449865924162914E-4	0.23338023224855858
>5k	2.9312332388785278E-5	0.1226897915225151
>10k+	5.8624664777570556E-5	0.43494075708127233
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12245	0.21820575528594308	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12113	0.21585351684594764	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6871	0.12244113879703675	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.7819988181783837E-5	0.0
2	0.0	0.0	0.0	1.7819988181783837E-5	0.0
3	0.0	0.0	0.0	1.7819988181783837E-5	0.0
4	0.0	0.0	0.0	3.5639976363567675E-5	0.0
5	0.0	0.0	0.0	3.5639976363567675E-5	0.0
6	0.0	0.0	0.0	3.5639976363567675E-5	0.0
7	0.0	1.7819988181783837E-5	0.0	3.5639976363567675E-5	0.0
8	0.0	1.7819988181783837E-5	0.0	7.127995272713535E-5	0.0
9	0.0	1.7819988181783837E-5	0.0	1.7819988181783837E-4	0.0
10-11	0.0	1.7819988181783837E-5	0.0	2.22749852272298E-4	0.0
12-13	0.0	2.6729982272675756E-5	0.0	2.672998227267576E-4	0.0
14-15	0.0	4.4549970454459593E-5	0.0	2.851198109085414E-4	0.0
16-17	0.0	5.345996454535151E-5	0.0	3.1184979318121716E-4	0.0
18-19	0.0	8.018994681802726E-5	0.0	3.207597872721091E-4	0.0
20-21	0.0	8.909994090891919E-5	0.0	4.098597281810283E-4	0.0
22-23	0.0	8.909994090891919E-5	0.0	5.791496159079747E-4	0.0
24-25	0.0	8.909994090891919E-5	0.0	0.001087019279088814	0.0
26-27	0.0	8.909994090891919E-5	0.0	0.0018621887649964111	0.0
28-29	0.0	8.909994090891919E-5	0.0	0.005640026259534584	0.0
30-31	0.0	8.909994090891919E-5	0.0	0.018087288004510597	0.0
32-33	0.0	8.909994090891919E-5	0.0	0.04556570978082128	0.0
34-35	0.0	8.909994090891919E-5	0.0	0.09022260016437157	0.0
36-37	0.0	8.909994090891919E-5	0.0	0.15943543426242002	0.0
38-39	0.0	8.909994090891919E-5	0.0	0.24902542484633822	0.0
40-41	0.0	1.0691992909070302E-4	0.0	0.3428209326411575	0.0
42-43	0.0	1.0691992909070302E-4	0.0	0.43443349188370817	0.0
44-45	0.0	1.0691992909070302E-4	0.0	0.5469043472930368	0.0
46-47	0.0	1.0691992909070302E-4	0.0	0.6798860090995988	0.0
48-49	0.0	1.0691992909070302E-4	0.0	0.8161019987611544	0.0
50-51	0.0	1.0691992909070302E-4	0.0	0.9482639411113543	0.0
52-53	0.0	1.0691992909070302E-4	0.0	1.0771202756538332	0.0
54-55	0.0	1.0691992909070302E-4	0.0	1.220090040836285	0.0
56-57	0.0	1.0691992909070302E-4	0.0	1.3849962114705126	0.0
58-59	0.0	1.2473991727248688E-4	0.0	1.561209164606082	0.0
60-61	0.0	1.2473991727248688E-4	0.0	1.7385447769971039	0.0
62-63	0.0	1.2473991727248688E-4	0.0	1.909589933559956	0.0
64-65	0.0	1.2473991727248688E-4	0.0	2.095024730579599	0.0
66-67	0.0	1.2473991727248688E-4	0.0	2.306539080303282	0.0
68-69	0.0	1.2473991727248688E-4	0.0	2.534412179177843	0.0
70-71	0.0	1.2473991727248688E-4	0.0	2.7746345298623796	0.0
72-73	0.0	1.2473991727248688E-4	0.0	3.0200068571314524	0.0
74-75	0.0	1.6037989363605455E-4	0.0	3.266279093803705	0.0
76-77	0.0	1.6037989363605455E-4	0.0	3.5288387996741077	0.0
78-79	0.0	1.6037989363605455E-4	0.0	3.819010577232185	0.0
80-81	0.0	1.6037989363605455E-4	0.0	4.130503970649767	0.0
82-83	0.0	1.6037989363605455E-4	0.0	4.446167241301886	0.0
84-85	0.0	1.6037989363605455E-4	0.0	4.76077913265128	0.0
86-87	0.0	1.6037989363605455E-4	0.0	5.093496131993366	0.0
88	0.0	1.6037989363605455E-4	0.0	5.359735665423306	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9065	0.0	58.396896	1
GTATCAA	15640	0.0	37.814907	1
TCAACGC	18755	0.0	31.121822	4
ATCAACG	18890	0.0	30.874529	3
CAACGCA	19410	0.0	30.09581	5
TATCAAC	19750	0.0	29.672892	2
AACGCAG	20090	0.0	29.145477	6
GTGGTAT	3885	0.0	27.348694	1
TGGTATC	3750	0.0	26.443058	2
ACGCAGA	23500	0.0	24.656332	7
GTACATG	14350	0.0	24.014372	1
CGCAGAG	24060	0.0	23.906527	8
TACATGG	14470	0.0	22.864653	2
GCAGAGT	26395	0.0	21.470938	9
ACATGGG	14525	0.0	21.451511	3
GAGTACT	19000	0.0	20.834517	12-13
CAGAGTA	25820	0.0	20.36438	10-11
AGAGTAC	24230	0.0	18.973566	10-11
GTACTTT	21095	0.0	18.624613	14-15
GAGTACA	10815	0.0	17.735882	1
>>END_MODULE