FastQCFastQC Report
Wed 25 May 2016
SRR2049343_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049343_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4239956
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41250.045.7017441
GTATCAA69600.037.3532371
ATCAACG76300.032.900963
TCAACGC75950.032.866894
CAACGCA80100.031.1053565
AACGCAG84750.029.4485996
TATCAAC87400.029.0989742
GTACATG122050.026.6166041
TACATGG124900.025.142352
ACGCAGA99800.024.5838457
CGCAGAG100700.024.0837978
ACATGGG130550.023.2260153
CATGGGG89750.020.270684
GCAGAGT116200.019.9004869
GAGTACA93200.018.7646031
CAGAGTA109600.017.71104610-11
AGAGTAC101050.017.55841810-11
AGTACAT96650.016.5861032
GTGGTAT27650.016.4925921
GTATAGG16850.016.1823081
Produced by FastQC (version 0.11.5)