##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049343_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4239956
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.83701316711777	31.0	31.0	34.0	28.0	34.0
2	31.051101237843035	31.0	31.0	34.0	28.0	34.0
3	31.10782776991082	31.0	31.0	34.0	28.0	34.0
4	34.71043024974787	37.0	35.0	37.0	32.0	37.0
5	34.74522612970512	37.0	35.0	37.0	32.0	37.0
6	34.71685484472009	37.0	35.0	37.0	32.0	37.0
7	34.666722484855974	37.0	35.0	37.0	32.0	37.0
8	34.62792326146781	37.0	35.0	37.0	32.0	37.0
9	36.18961847717288	39.0	37.0	39.0	32.0	39.0
10-11	36.153913507593	39.0	37.0	39.0	32.0	39.0
12-13	36.09633177797128	39.0	37.0	39.0	32.0	39.0
14-15	37.29518407738193	40.0	38.0	41.0	31.5	41.0
16-17	37.251652847340864	40.0	37.5	41.0	31.0	41.0
18-19	37.274565113411555	40.0	37.5	41.0	31.0	41.0
20-21	37.35401959359956	40.0	38.0	41.0	31.5	41.0
22-23	37.29922680801405	40.0	38.0	41.0	31.0	41.0
24-25	37.304180397155065	40.0	38.0	41.0	31.0	41.0
26-27	37.294867328811904	40.0	38.0	41.0	31.0	41.0
28-29	37.23547355208403	40.0	37.0	41.0	31.0	41.0
30-31	37.166870717526315	40.0	37.0	41.0	31.0	41.0
32-33	37.11483409261794	40.0	37.0	41.0	31.0	41.0
34-35	37.01016861967436	40.0	37.0	41.0	30.0	41.0
36-37	36.929769436286605	40.0	37.0	41.0	30.0	41.0
38-39	36.82433414403357	40.0	37.0	41.0	30.0	41.0
40-41	36.718256392283315	40.0	37.0	41.0	30.0	41.0
42-43	36.569700133680634	40.0	36.5	41.0	30.0	41.0
44-45	36.4383087937705	40.0	36.0	41.0	30.0	41.0
46-47	36.31276055694917	40.0	36.0	41.0	30.0	41.0
48-49	36.176709263020655	40.0	36.0	41.0	29.0	41.0
50-51	36.031610233691104	39.0	35.0	41.0	29.0	41.0
52-53	35.89059744959617	39.0	35.0	41.0	28.0	41.0
54-55	35.74824125533378	39.0	35.0	41.0	28.0	41.0
56-57	35.621383806813085	39.0	35.0	41.0	28.0	41.0
58-59	35.40837452086767	39.0	35.0	41.0	28.0	41.0
60-61	35.18947448982961	38.5	35.0	40.0	27.5	41.0
62-63	34.982477766278706	38.0	34.0	40.0	27.0	41.0
64-65	34.732778123169204	38.0	34.0	40.0	26.5	41.0
66-67	34.46951914123637	37.0	34.0	40.0	26.0	41.0
68-69	34.18455085382962	37.0	34.0	40.0	26.0	41.0
70-71	33.900595194855796	36.0	34.0	39.0	26.0	41.0
72-73	33.560656289829424	36.0	33.5	39.0	26.0	41.0
74-75	33.21237225103279	35.5	33.0	39.0	25.0	40.0
76-77	32.88351553648198	35.0	33.0	37.5	25.0	40.0
78-79	32.57712709754535	35.0	33.0	37.0	25.0	39.0
80-81	32.23469300153115	35.0	33.0	37.0	24.0	39.0
82-83	31.93564874258129	35.0	32.5	36.0	23.5	39.0
84-85	31.651646856712663	35.0	32.0	36.0	23.0	37.0
86-87	31.34694829380305	35.0	32.0	35.5	21.5	37.0
88-89	31.111657762486217	35.0	32.0	35.0	20.0	36.5
90-91	30.923894847021998	35.0	32.0	35.0	20.0	36.0
92-93	30.69719214067316	35.0	32.0	35.0	18.5	36.0
94-95	30.508586999487733	35.0	32.0	35.0	16.5	36.0
96-97	30.2664721048992	35.0	32.0	35.0	5.0	35.5
98-99	30.06800530477203	34.5	31.5	35.0	2.0	35.0
100	29.955089392437092	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.344057948765343
1101	2	0.3471023343186488
1101	3	0.36922838995022644
1101	4	0.3838485661388731
1101	5	0.3854202161565041
1101	6	0.5058265011178307
1101	7	0.4555593899454351
1101	8	0.48566024119575246
1101	9	0.6439254677065946
1101	10-11	0.5831525343604582
1101	12-13	0.6002718223231369
1101	14-15	0.7278492028373194
1101	16-17	0.800296270128193
1101	18-19	0.7419374194399353
1101	20-21	0.6776725744889305
1101	22-23	0.7205996001536263
1101	24-25	0.6817009874968178
1101	26-27	0.5990674518947401
1101	28-29	0.6806675415841141
1101	30-31	0.6834442752111372
1101	32-33	0.6375530827738274
1101	34-35	0.7084009667864564
1101	36-37	0.6602910978578365
1101	38-39	0.6557836882267623
1101	40-41	0.7011358348637771
1101	42-43	0.6530296280024999
1101	44-45	0.6043800446662502
1101	46-47	0.6586296637110323
1101	48-49	0.5877897760308457
1101	50-51	0.6056425063922788
1101	52-53	0.6111338731609592
1101	54-55	0.5774522231772323
1101	56-57	0.563128194755528
1101	58-59	0.6563635191020083
1101	60-61	0.7224637206163109
1101	62-63	0.6747541198128957
1101	64-65	0.6487387967270166
1101	66-67	0.5993921970229366
1101	68-69	0.5801933843661615
1101	70-71	0.5462706893153921
1101	72-73	0.6584831423383548
1101	74-75	0.6474306335345119
1101	76-77	0.6515358753881202
1101	78-79	0.6011595386097355
1101	80-81	0.5756178571417152
1101	82-83	0.5536011631415221
1101	84-85	0.5734067581028413
1101	86-87	0.6351211312446132
1101	88-89	0.6070028949192832
1101	90-91	0.6365371920561458
1101	92-93	0.6901992944105899
1101	94-95	0.709295711061813
1101	96-97	0.6393764975592546
1101	98-99	0.691046064411708
1101	100	0.7053815702717614
1102	1	0.17800411238173552
1102	2	0.22233652846026786
1102	3	0.20933280543127708
1102	4	0.2627221668980866
1102	5	0.1894554467392311
1102	6	0.33824050137086914
1102	7	0.3687050290300604
1102	8	0.34289299135565443
1102	9	0.4958969760948051
1102	10-11	0.3695886022305217
1102	12-13	0.5035872680480367
1102	14-15	0.5121455364035228
1102	16-17	0.43823395334688797
1102	18-19	0.46786973286987177
1102	20-21	0.38907759032392875
1102	22-23	0.42261550615865673
1102	24-25	0.5370992935794519
1102	26-27	0.4969107562111148
1102	28-29	0.5603363695483807
1102	30-31	0.5149263345710011
1102	32-33	0.5201032123435709
1102	34-35	0.5633336749592672
1102	36-37	0.4731528347089693
1102	38-39	0.47748475829844494
1102	40-41	0.510869796863858
1102	42-43	0.44042762962763504
1102	44-45	0.3060001559247212
1102	46-47	0.26485546890907585
1102	48-49	0.3230350118699974
1102	50-51	0.43709062838331647
1102	52-53	0.4000173176563422
1102	54-55	0.3966600288597135
1102	56-57	0.34424424770073614
1102	58-59	0.46751643711674973
1102	60-61	0.41380652135062945
1102	62-63	0.418760470761228
1102	64-65	0.45509683056270944
1102	66-67	0.4616072830964697
1102	68-69	0.5591537204498422
1102	70-71	0.5267240943160374
1102	72-73	0.455073262195846
1102	74-75	0.4888001546509244
1102	76-77	0.5074539067811301
1102	78-79	0.4571875048716407
1102	80-81	0.3929516860219948
1102	82-83	0.39666027727538733
1102	84-85	0.40048154683066883
1102	86-87	0.4273545139865469
1102	88-89	0.4522214220637757
1102	90-91	0.5234295225029761
1102	92-93	0.5072096737231426
1102	94-95	0.5018909804000486
1102	96-97	0.4337621055436358
1102	98-99	0.5471156525262977
1102	100	0.5754639754041797
1103	1	0.18256892213414844
1103	2	0.26379611156931304
1103	3	0.2920057276311816
1103	4	0.21821391270958657
1103	5	0.21981959888321256
1103	6	0.3351522543539218
1103	7	0.38843209871779294
1103	8	0.37874721331948535
1103	9	0.44171425018873123
1103	10-11	0.4062277516492614
1103	12-13	0.4381022881726153
1103	14-15	0.5132372970876844
1103	16-17	0.524520989593654
1103	18-19	0.4816558628135823
1103	20-21	0.4833666135362833
1103	22-23	0.491624335958754
1103	24-25	0.5253326631649102
1103	26-27	0.4521371178980047
1103	28-29	0.5001611002559585
1103	30-31	0.48887188934818226
1103	32-33	0.5262224550657564
1103	34-35	0.5153928067617883
1103	36-37	0.5035080153681903
1103	38-39	0.5572036254858261
1103	40-41	0.5061189379533388
1103	42-43	0.482607015944275
1103	44-45	0.447847217038138
1103	46-47	0.5321317458527517
1103	48-49	0.4738570928206798
1103	50-51	0.3948106262312834
1103	52-53	0.4670163658620865
1103	54-55	0.4777847713696559
1103	56-57	0.4192540430973679
1103	58-59	0.48396083067114404
1103	60-61	0.4644469050879323
1103	62-63	0.46268745160732294
1103	64-65	0.3989642503270687
1103	66-67	0.4497135871630391
1103	68-69	0.43537369449591523
1103	70-71	0.4358842056018801
1103	72-73	0.43019567296478556
1103	74-75	0.4108196710808514
1103	76-77	0.5156834765132317
1103	78-79	0.3777284163913919
1103	80-81	0.36006915219301305
1103	82-83	0.3746832099115096
1103	84-85	0.38270850032570536
1103	86-87	0.36190199764703834
1103	88-89	0.3690804193362709
1103	90-91	0.43792055482088443
1103	92-93	0.3789612427470992
1103	94-95	0.3973905766128141
1103	96-97	0.3198646559522551
1103	98-99	0.33591653542179856
1103	100	0.3657251681412461
1104	1	0.20084937997351204
1104	2	0.2027786398266045
1104	3	0.19796441515388352
1104	4	0.27161897993054396
1104	5	0.24842517302504064
1104	6	0.37401299997542736
1104	7	0.41300880258862804
1104	8	0.37286508804078977
1104	9	0.522089820220863
1104	10-11	0.4586506287289467
1104	12-13	0.44148591274026217
1104	14-15	0.518644958380861
1104	16-17	0.5040081957375335
1104	18-19	0.5766448778135285
1104	20-21	0.5034233789984128
1104	22-23	0.5154411541038542
1104	24-25	0.5514034175377347
1104	26-27	0.45365236461698544
1104	28-29	0.45433036785824754
1104	30-31	0.5230007074581735
1104	32-33	0.5472557547535715
1104	34-35	0.5683368764629222
1104	36-37	0.5421097604551619
1104	38-39	0.5881207857949704
1104	40-41	0.588794149397728
1104	42-43	0.6245514017572233
1104	44-45	0.5042163476218349
1104	46-47	0.4045973940434351
1104	48-49	0.420120548546862
1104	50-51	0.4298377922671861
1104	52-53	0.46445497675629355
1104	54-55	0.44800914989023966
1104	56-57	0.48911157623961543
1104	58-59	0.5245348339230773
1104	60-61	0.4434030880904132
1104	62-63	0.407207107758893
1104	64-65	0.47591877248341063
1104	66-67	0.5109074965391613
1104	68-69	0.46614732921377566
1104	70-71	0.44802852022031914
1104	72-73	0.539379420114976
1104	74-75	0.5759933735404204
1104	76-77	0.5495623166206869
1104	78-79	0.43936982988872586
1104	80-81	0.5146826003927885
1104	82-83	0.46365073517938526
1104	84-85	0.42648209542522864
1104	86-87	0.4533037481852311
1104	88-89	0.4501107154635875
1104	90-91	0.4930874666804712
1104	92-93	0.49825600286180816
1104	94-95	0.5256845582850076
1104	96-97	0.5097251641061078
1104	98-99	0.535953395831676
1104	100	0.5550169201168522
1105	1	0.1017616626749529
1105	2	0.11923954434336537
1105	3	0.1511825600925505
1105	4	0.1643573784202914
1105	5	0.17170572318296706
1105	6	0.1523947463903994
1105	7	0.12909809212437295
1105	8	0.2811215366346147
1105	9	0.2847864621130185
1105	10-11	0.2762719450907838
1105	12-13	0.26182390714362214
1105	14-15	0.33218522379003446
1105	16-17	0.2655414646367049
1105	18-19	0.23640213012154732
1105	20-21	0.22088075261228823
1105	22-23	0.22043636410322165
1105	24-25	0.16336560131710343
1105	26-27	0.1399863088135902
1105	28-29	0.21048619472519903
1105	30-31	0.2145823066514012
1105	32-33	0.17440368347860868
1105	34-35	0.2979585990288456
1105	36-37	0.27924664806914024
1105	38-39	0.20895944517197051
1105	40-41	0.16906621115652598
1105	42-43	0.2514703151462143
1105	44-45	0.20770629012573494
1105	46-47	0.16187557642492578
1105	48-49	0.18402957419320387
1105	50-51	0.1549138802736394
1105	52-53	0.17052276591861215
1105	54-55	0.15640868084025783
1105	56-57	0.17225040030419336
1105	58-59	0.13875513305143983
1105	60-61	0.09890347615390738
1105	62-63	0.04253577208082504
1105	64-65	0.04996311973506806
1105	66-67	0.1780354508811186
1105	68-69	0.12326489073104341
1105	70-71	0.12103684460143427
1105	72-73	0.16042625519803977
1105	74-75	0.2030948607737244
1105	76-77	0.246038205428313
1105	78-79	0.10290787897859133
1105	80-81	0.23438290730889122
1105	82-83	0.17300109046782097
1105	84-85	0.18941980745807996
1105	86-87	0.1903037248801418
1105	88-89	0.1216710099631122
1105	90-91	0.1212016872650743
1105	92-93	0.1477098618188819
1105	94-95	0.054911179113830144
1105	96-97	0.12022466797758113
1105	98-99	0.1586794690946789
1105	100	0.1886528251539552
1106	1	0.013418423413725122
1106	2	0.07109475814567645
1106	3	0.0405258191200204
1106	4	0.09122715081962696
1106	5	0.0513370675762701
1106	6	0.16477677614011554
1106	7	0.1419843130775007
1106	8	0.13851692380206515
1106	9	0.1371572707195483
1106	10-11	0.16791660280790666
1106	12-13	0.21008280537331814
1106	14-15	0.25243551002041187
1106	16-17	0.18143930053737023
1106	18-19	0.1551705564398418
1106	20-21	0.19415413341500454
1106	22-23	0.23666110322684375
1106	24-25	0.26045395793659054
1106	26-27	0.19782734870034346
1106	28-29	0.22919828725554225
1106	30-31	0.24089284646745313
1106	32-33	0.2141545832227152
1106	34-35	0.23841887876677959
1106	36-37	0.1789928039153139
1106	38-39	0.22191142688971865
1106	40-41	0.2854229279534195
1106	42-43	0.26593464240219333
1106	44-45	0.15356473038683305
1106	46-47	0.11582859663057832
1106	48-49	0.0918279646129605
1106	50-51	0.1332895361707429
1106	52-53	0.14608591373816893
1106	54-55	0.16644856303345534
1106	56-57	0.055991146282146076
1106	58-59	0.16272778265528132
1106	60-61	0.21637105571682724
1106	62-63	0.09449643117854123
1106	64-65	0.13478424285290203
1106	66-67	0.14094706385203182
1106	68-69	0.13836158566343926
1106	70-71	0.17525898978600196
1106	72-73	0.12225756705306878
1106	74-75	0.1667971234001655
1106	76-77	0.1905807461809772
1106	78-79	0.10934787247093425
1106	80-81	0.09589111714122822
1106	82-83	0.08979585758348918
1106	84-85	0.05909976160039676
1106	86-87	0.07693551173315782
1106	88-89	0.13184347802162222
1106	90-91	0.15120676566862912
1106	92-93	0.19018454962630216
1106	94-95	0.16679342705243982
1106	96-97	0.1414168156101141
1106	98-99	0.12554645901541406
1106	100	0.12116449103757176
1107	1	0.14141933065743473
1107	2	0.09352036157009991
1107	3	0.16856046418066128
1107	4	0.15635945020116537
1107	5	0.1415250392917855
1107	6	0.195718327778593
1107	7	0.2056212151906962
1107	8	0.2849087667037864
1107	9	0.26613980775895385
1107	10-11	0.264853618721439
1107	12-13	0.20470142613089592
1107	14-15	0.1859764284146337
1107	16-17	0.23455633423544953
1107	18-19	0.2004031832534423
1107	20-21	0.24133413811905058
1107	22-23	0.26617683104682754
1107	24-25	0.26368126271445647
1107	26-27	0.26769314771032526
1107	28-29	0.323283902333948
1107	30-31	0.2549863690780896
1107	32-33	0.32618428681612244
1107	34-35	0.2669559013933238
1107	36-37	0.27976277641911196
1107	38-39	0.28186499605212134
1107	40-41	0.34960253505963834
1107	42-43	0.31342740678808667
1107	44-45	0.23323683187769007
1107	46-47	0.22751681280335845
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2216	100	-2.9173520528407373
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	67477.0
3	11662.0
4	7854.0
5	6160.0
6	5645.0
7	5904.0
8	6669.0
9	7347.0
10	8410.0
11	9529.0
12	10552.0
13	11806.0
14	13567.0
15	14482.0
16	14973.0
17	15731.0
18	14672.0
19	13370.0
20	13369.0
21	13902.0
22	15435.0
23	18410.0
24	22100.0
25	27532.0
26	33534.0
27	39617.0
28	48249.0
29	59989.0
30	72325.0
31	86706.0
32	110460.0
33	146612.0
34	202042.0
35	293097.0
36	477835.0
37	831772.0
38	1086498.0
39	393494.0
40	1168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.394385267673066	20.149926544060826	14.145953224088265	23.309734964177846
2	15.28560575316868	20.05483252956332	40.92849592767014	23.731065789597864
3	18.2755758446319	25.246844486557805	30.26253947341101	26.21504019539928
4	12.489444209150358	16.751777584561353	39.01012105389204	31.74865715239624
5	13.023448531021828	37.85247359161576	35.674371786256316	13.449706091106094
6	30.899095522395807	37.95471925518218	17.695982928348	13.450202294074023
7	26.96755034593475	32.5466993627288	22.555705487223783	17.93004480411266
8	24.75728178247238	36.554892493313275	20.523102942536635	18.16472278167771
9	24.76221823471327	17.0522399064705	21.686079228735547	36.49946263008068
10-11	23.78019776189483	27.118349933038054	29.010579421399697	20.09087288366742
12-13	24.14480004426433	24.633005405274037	29.059697976169225	22.162496574292405
14-15	21.608837143182413	26.828978251034187	26.261184605193538	25.30100000058987
16-17	21.17611945580719	30.187199731029786	26.907237717174283	21.729443095988746
18-19	21.0431267234303	29.13610884800592	28.92502071006685	20.895743718496927
20-21	22.19865672747555	28.32422906699044	28.503133091381322	20.973981114152682
22-23	21.801229940442457	28.20820790825656	28.54069152061203	21.44987063068896
24-25	21.64233255886518	28.33238073160992	28.44198596519789	21.583300744327005
26-27	21.350447735980225	28.31041911183272	28.59721504409881	21.741918108088246
28-29	21.45447767532725	28.361134967298675	28.660838154380468	21.52354920299361
30-31	21.706423522736053	28.64899044266454	28.26856094946934	21.376025085130067
32-33	21.357385032453514	28.420878781229575	28.660050915800177	21.56168527051673
34-35	21.58393506755951	28.13468937206941	28.65276534671488	21.628610213656202
36-37	21.83505901113831	28.462199711004853	28.161620222534996	21.541121055321845
38-39	21.663124108904572	28.61609267321668	28.26872618682763	21.452057031051115
40-41	21.584744964924404	28.344580063736057	28.46194699577696	21.608727975562577
42-43	21.49608970682298	28.356273043620643	28.404628224278383	21.743009025278
44-45	21.633801241792	28.42207385374657	28.30739807970869	21.636726824752746
46-47	21.678409204183314	28.549861006498162	28.142653988461742	21.629075800856786
48-49	21.60438645229863	28.42289911183307	28.162264760805993	21.810449675062316
50-51	21.645643656137583	28.32223375945599	28.163248790654887	21.86887379375154
52-53	21.603446268373062	28.34540872884938	28.221282856579645	21.82986214619791
54-55	21.733933723435115	28.592510994247565	28.019072464062177	21.65448281825514
56-57	21.635602095723122	28.44414240102186	28.049536903889244	21.87071859936577
58-59	21.57310878214198	28.111850445169384	28.336387480568003	21.978653292120633
60-61	21.78391383307961	28.47679642705262	27.99806772061222	21.741222019255556
62-63	21.632671839471985	28.48417929639231	28.034068555890773	21.849080308244933
64-65	21.67672159512692	28.239118046153777	28.15611961824026	21.928040740479037
66-67	21.55033450404539	28.329525265309428	28.028115963604392	22.092024267040784
68-69	21.881834228274773	28.389774275161137	27.98829221014614	21.740099286417948
70-71	21.853229346958265	28.254874833195863	28.04263013553839	21.849265684307483
72-73	21.705146354775955	28.372148822441446	28.14540057421971	21.77730424856289
74-75	21.78503923994742	28.350117367721406	28.024396726137148	21.840446666194026
76-77	21.801324290819274	28.402346970517538	27.846900269796148	21.949428468867037
78-79	21.851856832576708	28.45859043703973	27.95906960898531	21.730483121398247
80-81	21.823736238535897	28.275684389758116	28.03767886098972	21.862900510716266
82-83	21.698325986200683	28.372451850427556	28.148900053437266	21.780322109934495
84-85	21.795917596965477	28.225888170680708	28.03352319745574	21.944671034898075
86-87	21.93473262492275	28.401284448842702	27.905430855532064	21.75855207070249
88-89	21.732327418309513	28.249895187693657	28.012300965735992	22.00547642826084
90-91	21.719088543660416	28.143156793059642	28.037966612587173	22.099788050692773
92-93	21.840301591784222	28.34650638645942	27.91683388080481	21.89635814095155
94-95	21.739161721346537	28.146977753314307	28.032657161617063	22.081203363722093
96-97	21.772053766595693	28.174808889526208	27.986976751645535	22.06616059223256
98-99	21.786983166806447	28.255080948953243	27.917707164885673	22.040228719354634
100	21.833080343286582	28.135268384860595	27.96878080810273	22.062870463750095
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1572.0
1	1229.0
2	1026.5
3	1198.0
4	1143.0
5	927.0
6	718.0
7	654.0
8	693.0
9	715.5
10	637.5
11	557.0
12	568.0
13	654.5
14	733.5
15	817.5
16	1022.0
17	1317.0
18	1762.5
19	2485.5
20	3555.0
21	5088.5
22	7365.0
23	10757.0
24	15612.0
25	21996.5
26	30190.0
27	40185.0
28	52105.0
29	66250.0
30	79734.0
31	91190.0
32	101214.5
33	111027.5
34	120882.0
35	128627.5
36	135308.5
37	142570.5
38	148270.0
39	150589.0
40	151860.5
41	152755.5
42	152356.5
43	150857.0
44	147372.0
45	143975.5
46	141222.5
47	136455.5
48	131033.5
49	127781.5
50	125292.5
51	120059.5
52	112205.0
53	106215.0
54	102278.5
55	97830.5
56	91336.5
57	83212.0
58	76491.5
59	69998.0
60	61499.5
61	52198.5
62	44211.0
63	37329.0
64	30616.5
65	24833.0
66	20201.5
67	15838.0
68	12240.0
69	9562.5
70	7187.0
71	5338.0
72	4145.0
73	3142.5
74	2356.0
75	1778.5
76	1307.0
77	956.5
78	691.0
79	478.0
80	320.5
81	204.0
82	145.0
83	109.0
84	88.0
85	72.5
86	55.0
87	36.5
88	31.5
89	25.5
90	21.0
91	22.0
92	20.5
93	24.5
94	19.5
95	9.0
96	8.0
97	11.0
98	13.5
99	14.5
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016037902280118
2	0.03764190005745342
3	0.03915134968381747
4	0.041533449875423234
5	0.045000466985978156
6	0.04290138859931565
7	0.04077872506224121
8	0.040283436903590514
9	0.040590043858945705
10-11	0.040755139911829275
12-13	0.04199336030845603
14-15	0.040295229478796483
16-17	0.03699330842112512
18-19	0.0395994675416443
20-21	0.03891549817969809
22-23	0.040141926001118884
24-25	0.03687538266906543
26-27	0.03718198962442063
28-29	0.03764190005745342
30-31	0.037465011429363884
32-33	0.03740604855333404
34-35	0.03729991537648032
36-37	0.03482347458322681
38-39	0.03521262956502379
40-41	0.036297546483972945
42-43	0.036014524679029686
44-45	0.035012155786522316
46-47	0.03416309037169253
48-49	0.03422205324772239
50-51	0.035495651369967046
52-53	0.035766880599704336
54-55	0.035130081538582006
56-57	0.03346732843454036
58-59	0.03436356415019401
60-61	0.03392723886757315
62-63	0.03404516461963285
64-65	0.031167776269376377
66-67	0.03225269318832554
68-69	0.03326685465603888
70-71	0.03424563839813432
72-73	0.03147438322473158
74-75	0.03194608623297034
76-77	0.032677225895740424
78-79	0.0328776996742419
80-81	0.03299562542630159
82-83	0.03162768670240918
84-85	0.03298383285109562
86-87	0.03317251405439113
88-89	0.03365600963783587
90-91	0.03402157946922091
92-93	0.03252392241806283
94-95	0.03389186114195525
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4239956.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.3119445224589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.95493651489942	52.35655369950275
2	13.074814048141242	17.34032687603617
3	3.77821158159375	7.516216703782693
4	1.5671314382058625	4.156781319588335
5	0.7779793513108251	2.5794661791870994
6	0.44169383265330703	1.7573746156091006
7	0.2932381232966698	1.3611633114743114
8	0.20572561994400104	1.0913652717260052
9	0.15064665579572167	0.8990705413457872
>10	0.7111028416862222	8.33027272233902
>50	0.03518223464396832	1.5823441636489208
>100	0.009231018638449518	0.9421221394611413
>500	7.115946036161013E-5	0.0432942760720432
>1k	3.5579730172533425E-5	0.043648180226514226
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.3585150411938237E-5	0.0	0.0	0.0
3	0.0	2.3585150411938237E-5	0.0	0.0	0.0
4	0.0	2.3585150411938237E-5	0.0	2.3585150411938237E-5	0.0
5	0.0	2.3585150411938237E-5	0.0	4.7170300823876474E-5	0.0
6	0.0	2.3585150411938237E-5	0.0	7.075545123581471E-5	0.0
7	0.0	2.3585150411938237E-5	0.0	1.4151090247162943E-4	0.0
8	0.0	2.3585150411938237E-5	0.0	1.4151090247162943E-4	0.0
9	7.075545123581471E-5	2.3585150411938237E-5	0.0	3.5377725617907356E-4	0.0
10-11	7.075545123581471E-5	2.3585150411938237E-5	0.0	3.891549817969809E-4	0.0
12-13	7.075545123581471E-5	3.537772561790736E-5	0.0	4.363252826208574E-4	0.0
14-15	7.075545123581471E-5	4.7170300823876474E-5	0.0	4.717030082387647E-4	0.0
16-17	7.075545123581471E-5	4.7170300823876474E-5	0.0	5.424584594745795E-4	0.0
18-19	7.075545123581471E-5	4.7170300823876474E-5	0.0	6.01421335504425E-4	0.0
20-21	7.075545123581471E-5	4.7170300823876474E-5	0.0	7.075545123581471E-4	0.0
22-23	7.075545123581471E-5	5.89628760298456E-5	0.0	8.018951140059E-4	0.0
24-25	9.434060164775295E-5	7.075545123581471E-5	0.0	9.90576317301406E-4	0.0
26-27	1.1792575205969118E-4	7.075545123581471E-5	0.0	0.001285390697450634	0.0
28-29	1.1792575205969118E-4	7.075545123581471E-5	0.0	0.0027122922973728974	0.0
30-31	1.1792575205969118E-4	7.075545123581471E-5	0.0	0.007665173883879927	0.0
32-33	1.1792575205969118E-4	7.075545123581471E-5	0.0	0.018632268825431206	0.0
34-35	1.1792575205969118E-4	7.075545123581471E-5	0.0	0.0341395052212806	0.0
36-37	1.1792575205969118E-4	7.075545123581471E-5	0.0	0.060625629133887235	0.0
38-39	1.1792575205969118E-4	7.075545123581471E-5	0.0	0.09360946198498286	0.0
40-41	1.1792575205969118E-4	7.075545123581471E-5	0.0	0.12851548459465145	0.0
42-43	1.1792575205969118E-4	7.075545123581471E-5	0.0	0.1643884983712095	0.0
44-45	1.1792575205969118E-4	7.075545123581471E-5	0.0	0.20774979740355798	0.0
46-47	1.886812032955059E-4	9.434060164775295E-5	0.0	0.25780927915289686	0.0
48-49	1.886812032955059E-4	9.434060164775295E-5	0.0	0.30903622584762674	0.0
50-51	1.886812032955059E-4	9.434060164775295E-5	0.0	0.3615485632398072	0.0
52-53	2.1226635370744413E-4	9.434060164775295E-5	0.0	0.4136363679245728	0.0
54-55	2.1226635370744413E-4	9.434060164775295E-5	0.0	0.4712784755313498	0.0
56-57	2.1226635370744413E-4	9.434060164775295E-5	0.0	0.5381305843739888	0.0
58-59	2.1226635370744413E-4	9.434060164775295E-5	0.0	0.6110912471733196	0.0
60-61	2.1226635370744413E-4	1.1792575205969118E-4	0.0	0.6834269034867343	0.0
62-63	2.1226635370744413E-4	1.297183272656603E-4	0.0	0.758356926345462	0.0
64-65	2.1226635370744413E-4	1.4151090247162943E-4	0.0	0.8363058484569179	0.0
66-67	2.1226635370744413E-4	1.4151090247162943E-4	0.0	0.9269317889147906	0.0
68-69	2.1226635370744413E-4	2.2405892891341325E-4	0.0	1.026484708803582	0.0
70-71	2.2405892891341325E-4	2.594366545313206E-4	0.0	1.131143813756558	0.0
72-73	2.3585150411938236E-4	2.7122922973728974E-4	0.0	1.2388925734134977	0.0
74-75	2.3585150411938236E-4	2.8302180494325886E-4	0.0	1.3484455970769509	0.0
76-77	2.3585150411938236E-4	3.066069553551971E-4	0.0	1.4686944864522178	0.0
78-79	2.3585150411938236E-4	3.066069553551971E-4	0.0	1.5998397153178003	0.0
80-81	2.3585150411938236E-4	3.066069553551971E-4	0.0	1.74439310219257	0.0
82-83	2.3585150411938236E-4	3.066069553551971E-4	0.0	1.8892766811731065	0.0
84-85	2.3585150411938236E-4	3.066069553551971E-4	0.0	2.0336413868445806	0.0
86-87	2.3585150411938236E-4	3.066069553551971E-4	0.0	2.186142969408173	0.0
88	2.594366545313206E-4	3.066069553551971E-4	0.0	2.3088682995766936	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4125	0.0	45.701744	1
GTATCAA	6960	0.0	37.353237	1
ATCAACG	7630	0.0	32.90096	3
TCAACGC	7595	0.0	32.86689	4
CAACGCA	8010	0.0	31.105356	5
AACGCAG	8475	0.0	29.448599	6
TATCAAC	8740	0.0	29.098974	2
GTACATG	12205	0.0	26.616604	1
TACATGG	12490	0.0	25.14235	2
ACGCAGA	9980	0.0	24.583845	7
CGCAGAG	10070	0.0	24.083797	8
ACATGGG	13055	0.0	23.226015	3
CATGGGG	8975	0.0	20.27068	4
GCAGAGT	11620	0.0	19.900486	9
GAGTACA	9320	0.0	18.764603	1
CAGAGTA	10960	0.0	17.711046	10-11
AGAGTAC	10105	0.0	17.558418	10-11
AGTACAT	9665	0.0	16.586103	2
GTGGTAT	2765	0.0	16.492592	1
GTATAGG	1685	0.0	16.182308	1
>>END_MODULE