FastQCFastQC Report
Wed 25 May 2016
SRR2049343_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049343_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4239956
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100030.23592225957061816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92430.21799754525754514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54210.12785510038311718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67150.058.87831
GTATCAA121300.039.764161
ATCAACG152500.031.2793713
TCAACGC152900.031.2590184
CAACGCA158500.030.1545985
TATCAAC160450.029.817412
AACGCAG165200.029.0141776
GTGGTAT31950.028.6925411
TGGTATC30400.027.3628642
ACGCAGA189100.025.2230557
CGCAGAG191150.024.903338
GTACATG113450.023.4954551
TACATGG112900.022.3533272
GCAGAGT212300.022.0011489
GAGTACT149500.021.30711412-13
ACATGGG117550.020.6694893
AGAGTAC195700.020.2860610-11
CAGAGTA208200.020.14126610-11
GTACTTT169300.018.7512514-15
AGTACTT159550.018.52102312-13
Produced by FastQC (version 0.11.5)