##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049343_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4239956 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.855704870522242 31.0 31.0 34.0 30.0 34.0 2 32.040840989859326 33.0 31.0 34.0 30.0 34.0 3 32.021462015171856 34.0 31.0 34.0 30.0 34.0 4 35.70285823720812 37.0 35.0 37.0 33.0 37.0 5 35.790082255570574 37.0 35.0 37.0 35.0 37.0 6 35.835612916737816 37.0 35.0 37.0 35.0 37.0 7 35.77119880489326 37.0 35.0 37.0 33.0 37.0 8 35.77852671112625 37.0 35.0 37.0 33.0 37.0 9 37.520649742591665 39.0 37.0 39.0 35.0 39.0 10-11 37.478288689788286 39.0 37.0 39.0 34.5 39.0 12-13 37.4252465119921 39.0 37.0 39.0 34.0 39.0 14-15 38.715986557407675 40.0 38.0 41.0 34.0 41.0 16-17 38.74415878372323 40.0 38.0 41.0 34.5 41.0 18-19 38.81472178013168 40.0 38.0 41.0 35.0 41.0 20-21 38.843071956407094 40.0 38.0 41.0 35.0 41.0 22-23 38.81379500164624 40.0 38.0 41.0 35.0 41.0 24-25 38.79381448769752 40.0 38.0 41.0 35.0 41.0 26-27 38.67508990659337 40.0 38.0 41.0 34.0 41.0 28-29 38.59615630445222 40.0 38.0 41.0 34.0 41.0 30-31 38.49269615062043 40.0 38.0 41.0 34.0 41.0 32-33 38.35644520839367 40.0 38.0 41.0 34.0 41.0 34-35 38.2550930953057 40.0 38.0 41.0 34.0 41.0 36-37 38.144739591637276 40.0 38.0 41.0 34.0 41.0 38-39 38.05155018118113 40.0 38.0 41.0 33.0 41.0 40-41 37.93542999031122 40.0 37.5 41.0 33.0 41.0 42-43 37.79697878940253 40.0 37.0 41.0 33.0 41.0 44-45 37.62030903150882 40.0 37.0 41.0 33.0 41.0 46-47 37.386458255698884 40.0 37.0 41.0 32.5 41.0 48-49 37.180050217502256 40.0 37.0 41.0 31.5 41.0 50-51 37.035182676424 40.0 37.0 41.0 31.0 41.0 52-53 36.897351411193895 40.0 36.0 41.0 31.0 41.0 54-55 36.75358364567934 40.0 36.0 41.0 31.0 41.0 56-57 36.611763188108554 39.0 35.5 41.0 31.0 41.0 58-59 36.41658899290464 39.0 35.0 41.0 30.5 41.0 60-61 36.238435493198516 39.0 35.0 41.0 30.0 41.0 62-63 36.032516611021435 39.0 35.0 41.0 30.0 41.0 64-65 35.80978033734312 38.0 35.0 40.0 30.0 41.0 66-67 35.541100426513864 37.5 35.0 40.0 29.5 41.0 68-69 35.25754512546828 37.0 35.0 40.0 29.0 41.0 70-71 34.932145993967865 37.0 34.0 39.5 29.0 41.0 72-73 34.63695389763479 36.0 34.0 39.0 29.0 41.0 74-75 34.331492001332094 36.0 34.0 39.0 29.0 40.5 76-77 34.0099968018536 35.0 34.0 38.5 29.0 40.0 78-79 33.67821706168649 35.0 34.0 37.0 28.5 39.0 80-81 33.3628306520162 35.0 34.0 37.0 28.0 39.0 82-83 33.06443698944045 35.0 34.0 36.5 28.0 39.0 84-85 32.79254890852641 35.0 33.0 36.0 27.0 37.0 86-87 32.56224651859595 35.0 33.0 36.0 27.0 37.0 88-89 32.33550808074423 35.0 33.0 35.0 27.0 37.0 90-91 32.14360927330378 35.0 33.0 35.0 26.5 36.0 92-93 31.961287805816852 35.0 33.0 35.0 26.0 36.0 94-95 31.803191589724044 35.0 33.0 35.0 26.0 36.0 96-97 31.600934302148417 35.0 33.0 35.0 25.5 36.0 98-99 31.45219915017986 35.0 33.0 35.0 25.0 35.0 100 31.33840728535862 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2181167774346271 1101 2 0.17984139263019472 1101 3 0.3488120977051068 1101 4 0.1832602587754053 1101 5 0.15634806522818678 1101 6 0.12460349995654951 1101 7 0.021948020223646836 1101 8 0.06425970014632298 1101 9 0.23388726604533616 1101 10-11 0.2679278560547047 1101 12-13 0.15490744041881044 1101 14-15 0.2834348375291995 1101 16-17 0.2160646089095124 1101 18-19 0.3220868450587986 1101 20-21 0.32058571363759114 1101 22-23 0.30341782411161944 1101 24-25 0.2893769090656022 1101 26-27 0.35994193501615257 1101 28-29 0.343054637419705 1101 30-31 0.3111339808605038 1101 32-33 0.3913824378063566 1101 34-35 0.3906438274048156 1101 36-37 0.41666712929762184 1101 38-39 0.3596958392457097 1101 40-41 0.40792375295238514 1101 42-43 0.410621066520072 1101 44-45 0.4404670848729282 1101 46-47 0.4821343642354563 1101 48-49 0.6062835914844769 1101 50-51 0.5062015211907536 1101 52-53 0.5718625858834088 1101 54-55 0.5897607833113412 1101 56-57 0.535752256234602 1101 58-59 0.5666529569324936 1101 60-61 0.5805582530583209 1101 62-63 0.5385374151741189 1101 64-65 0.4720021882038381 1101 66-67 0.48831262025090894 1101 68-69 0.5609139797447611 1101 70-71 0.5143948216597067 1101 72-73 0.46396509776133144 1101 74-75 0.512527975474697 1101 76-77 0.4487241602998324 1101 78-79 0.5001734811824363 1101 80-81 0.5381560011445785 1101 82-83 0.5112541243535489 1101 84-85 0.5037556661241851 1101 86-87 0.434649952982717 1101 88-89 0.523100896079093 1101 90-91 0.4737852047216933 1101 92-93 0.4598499197156869 1101 94-95 0.5129060572061093 1101 96-97 0.530339936620237 1101 98-99 0.5742799766086222 1101 100 0.5951466938250682 1102 1 0.17089579943650435 1102 2 0.1391821457271405 1102 3 0.33155622396906637 1102 4 0.22392049910983047 1102 5 0.18919236735872147 1102 6 0.15135873979571812 1102 7 0.12734308304503372 1102 8 0.17607522227778816 1102 9 0.15429523909179466 1102 10-11 0.22818008243667975 1102 12-13 0.24800702227186378 1102 14-15 0.25246338728594253 1102 16-17 0.18381779731289782 1102 18-19 0.19147682852401005 1102 20-21 0.17813133624294863 1102 22-23 0.2353440495603465 1102 24-25 0.179701983185808 1102 26-27 0.25224304640288864 1102 28-29 0.24647470023379014 1102 30-31 0.23748897012121262 1102 32-33 0.24144064119741415 1102 34-35 0.15629621524860937 1102 36-37 0.20242542861323187 1102 38-39 0.254386240741006 1102 40-41 0.2792344191036378 1102 42-43 0.22314250929797907 1102 44-45 0.27628912449856813 1102 46-47 0.17734922444943635 1102 48-49 0.09213606225056736 1102 50-51 0.19230807663986127 1102 52-53 0.2910499239577433 1102 54-55 0.22625756736945846 1102 56-57 0.32620240044285964 1102 58-59 0.3347537732701156 1102 60-61 0.3127467151710377 1102 62-63 0.28856566953111695 1102 64-65 0.27386653748730083 1102 66-67 0.2656136045368669 1102 68-69 0.31984372982957154 1102 70-71 0.33095545413655714 1102 72-73 0.33323920037869925 1102 74-75 0.3036575975237241 1102 76-77 0.2737821080283922 1102 78-79 0.2370077105032209 1102 80-81 0.28333895468033177 1102 82-83 0.308148628661975 1102 84-85 0.2208078718194102 1102 86-87 0.2854327769048979 1102 88-89 0.23100786473721513 1102 90-91 0.28249211938388896 1102 92-93 0.32410314712586086 1102 94-95 0.30209219147537425 1102 96-97 0.282114643331532 1102 98-99 0.3165654414512318 1102 100 0.30736423425845416 1103 1 0.16965593179195437 1103 2 0.17661830664149392 1103 3 0.3487964882180208 1103 4 0.21098035060288112 1103 5 0.1334514198966943 1103 6 0.14069630620512896 1103 7 0.16906367016603951 1103 8 0.18654171976788092 1103 9 0.17200987940003643 1103 10-11 0.2036675072881664 1103 12-13 0.2208343103455377 1103 14-15 0.27502460112079063 1103 16-17 0.2572644558502972 1103 18-19 0.25652744488051127 1103 20-21 0.29024427723648927 1103 22-23 0.2625295919774402 1103 24-25 0.32291883650724174 1103 26-27 0.24830314211187243 1103 28-29 0.36455394618964476 1103 30-31 0.26932662878058267 1103 32-33 0.27057631962334483 1103 34-35 0.30424212550502716 1103 36-37 0.2930160311730745 1103 38-39 0.22517348078272192 1103 40-41 0.18646901314887998 1103 42-43 0.31147955093891966 1103 44-45 0.35148823653283756 1103 46-47 0.31214358590646896 1103 48-49 0.3553583125468194 1103 50-51 0.31283143954106407 1103 52-53 0.3276625955250694 1103 54-55 0.2741057067511221 1103 56-57 0.28558898186210513 1103 58-59 0.32944689356378376 1103 60-61 0.3187747360614992 1103 62-63 0.3232858366683331 1103 64-65 0.35900789963755386 1103 66-67 0.3367889000254678 1103 68-69 0.36769243256977546 1103 70-71 0.3719085920013754 1103 72-73 0.33038291624093574 1103 74-75 0.31897497823325693 1103 76-77 0.3469541996969667 1103 78-79 0.3316454526523245 1103 80-81 0.354731692481721 1103 82-83 0.3644536106950653 1103 84-85 0.41642430859354107 1103 86-87 0.38744986807630966 1103 88-89 0.39576726697999476 1103 90-91 0.36212401809653727 1103 92-93 0.422076901378162 1103 94-95 0.37713581655238215 1103 96-97 0.4743821389239571 1103 98-99 0.483006116839249 1103 100 0.48258113433864835 1104 1 0.19282969926762306 1104 2 0.174221706676299 1104 3 0.27240817707792075 1104 4 0.1897382129652101 1104 5 0.17211722496092818 1104 6 0.2137859257766621 1104 7 0.1919404751403988 1104 8 0.2110249932997874 1104 9 0.19121017764454962 1104 10-11 0.17588155611385758 1104 12-13 0.192688885336203 1104 14-15 0.24347897432454602 1104 16-17 0.23229124536381107 1104 18-19 0.21224083130422144 1104 20-21 0.25687584311146594 1104 22-23 0.25415837904722594 1104 24-25 0.3090254434596531 1104 26-27 0.2859116410892497 1104 28-29 0.2758819963771231 1104 30-31 0.3350383757967563 1104 32-33 0.3551969371001178 1104 34-35 0.34996371219834543 1104 36-37 0.3296096164975566 1104 38-39 0.3028013800892211 1104 40-41 0.32441690207434704 1104 42-43 0.3267875423409521 1104 44-45 0.31767035911553876 1104 46-47 0.4418125475910628 1104 48-49 0.41214685620430913 1104 50-51 0.3362370456899839 1104 52-53 0.2894239871517925 1104 54-55 0.33064187537518563 1104 56-57 0.3188712171402841 1104 58-59 0.38714319820669374 1104 60-61 0.3622607682527672 1104 62-63 0.3745714303214456 1104 64-65 0.3185067768979053 1104 66-67 0.31304120524873724 1104 68-69 0.3183839180763428 1104 70-71 0.346114964025908 1104 72-73 0.3466462052522701 1104 74-75 0.3409077974295016 1104 76-77 0.26781541680637133 1104 78-79 0.3283426403452552 1104 80-81 0.33562926850981256 1104 82-83 0.3543013903455474 1104 84-85 0.39937456451968245 1104 86-87 0.34871002438372756 1104 88-89 0.2811854735931263 1104 90-91 0.2707226446193829 1104 92-93 0.42660662990741116 1104 94-95 0.37678699469171306 1104 96-97 0.41614139853982834 1104 98-99 0.5075619207553359 1104 100 0.48958221518411804 1105 1 0.104508752148746 1105 2 0.0873906763167227 1105 3 0.23770801315853163 1105 4 0.11076649999900923 1105 5 0.09848787960611105 1105 6 0.0794683857685996 1105 7 0.10591824561349483 1105 8 0.08329272446409419 1105 9 0.10909819455626035 1105 10-11 0.10279676612153565 1105 12-13 0.12074038719876512 1105 14-15 0.14109898477083505 1105 16-17 0.12818404495762792 1105 18-19 0.07804158149975393 1105 20-21 0.15359070512067063 1105 22-23 0.09845481107937815 1105 24-25 0.13021757721821103 1105 26-27 0.21542448632055056 1105 28-29 0.16157919515194408 1105 30-31 0.16268239931009276 1105 32-33 0.18943672945746926 1105 34-35 0.2173174041113839 1105 36-37 0.19665162228832855 1105 38-39 0.18236291686945805 1105 40-41 0.14032319214943811 1105 42-43 0.1876000688738486 1105 44-45 0.18388452812270373 1105 46-47 0.19752864009867466 1105 48-49 0.20897462073694584 1105 50-51 0.21004794756220946 1105 52-53 0.1725255879519949 1105 54-55 0.12745502880983395 1105 56-57 0.14097589571765923 1105 58-59 0.2009777228246321 1105 60-61 0.1724628778876962 1105 62-63 0.15349452700014865 1105 64-65 0.10384101835120418 1105 66-67 0.1405093376135511 1105 68-69 0.18042024733875905 1105 70-71 0.14140014177548466 1105 72-73 0.17282260496041602 1105 74-75 0.1811703855911304 1105 76-77 0.15643096829849412 1105 78-79 0.15824510361027677 1105 80-81 0.13857892738609223 1105 82-83 0.13014255748525017 1105 84-85 0.10708087283398271 1105 86-87 0.06026434572106609 1105 88-89 0.04650328936944703 1105 90-91 0.07377473689487601 1105 92-93 0.1318314391676978 1105 94-95 0.16184450765861413 1105 96-97 0.1461212616958676 1105 98-99 0.17337108725474337 1105 100 0.17453932940558659 1106 1 0.04842204220921431 1106 2 0.08502861047080756 1106 3 0.21748015400972065 1106 4 0.09640267647809253 1106 5 0.08598710036005741 1106 6 0.08797592811016841 1106 7 0.09163607187431921 1106 8 0.07867183337152284 1106 9 0.1758355635478921 1106 10-11 0.15919707508309244 1106 12-13 0.11421258598025474 1106 14-15 0.14073733857743065 1106 16-17 0.19161003634866347 1106 18-19 0.19426229135110162 1106 20-21 0.1699982819771435 1106 22-23 0.14340172351644753 1106 24-25 0.15068377162375413 1106 26-27 0.17590203871250054 1106 28-29 0.1583912045120215 1106 30-31 0.19667774968192475 1106 32-33 0.12500372409169103 1106 34-35 0.15535837192737745 1106 36-37 0.1407450892414559 1106 38-39 0.19219989099240564 1106 40-41 0.176001485927479 1106 42-43 0.17063531208224703 1106 44-45 0.13088652147212798 1106 46-47 0.0737329743792614 1106 48-49 -0.004566851118205761 1106 50-51 0.1578464444155756 1106 52-53 0.061552967237268774 1106 54-55 0.05295743344912296 1106 56-57 0.03675724763734678 1106 58-59 0.057654150973583285 1106 60-61 0.027877307548003216 1106 62-63 0.08104608267936442 1106 64-65 0.05714213271107127 1106 66-67 0.07232766456846207 1106 68-69 0.09131228260781654 1106 70-71 0.1401382193108489 1106 72-73 0.22278459295775122 1106 74-75 0.17452832029259469 1106 76-77 0.11956441931699402 1106 78-79 0.1370766754674122 1106 80-81 0.21589883792104558 1106 82-83 0.25039808937199126 1106 84-85 0.13623357008967218 1106 86-87 0.19759707390331727 1106 88-89 0.19792873946956746 1106 90-91 0.11506162564286626 1106 92-93 0.1970464274524062 1106 94-95 0.2044025177758897 1106 96-97 0.24287237582766252 1106 98-99 0.2156083520934864 1106 100 0.18069484106994693 1107 1 0.08973433222708849 1107 2 0.07360057283944599 1107 3 0.2022788611627604 1107 4 0.15122381545396024 1107 5 0.1327684953484507 1107 6 0.1157003678884081 1107 7 0.09212185201382539 1107 8 0.10101490624742837 1107 9 0.08866151170518322 1107 10-11 0.10303082884342274 1107 12-13 0.10298908540656271 1107 14-15 0.16406447224755283 1107 16-17 0.09027940401510648 1107 18-19 0.08745022629313581 1107 20-21 0.09752868611769117 1107 22-23 0.03750051781522501 1107 24-25 0.09714253485511648 1107 26-27 0.14303089125898794 1107 28-29 0.1793448815268448 1107 30-31 0.1546397510773403 1107 32-33 0.15869076153869344 1107 34-35 0.12885952560276337 1107 36-37 0.11081647421709562 1107 38-39 0.12340445939391742 1107 40-41 0.17992048999305155 1107 42-43 0.22694513531534 1107 44-45 0.27879202498127853 1107 46-47 0.2614678094526752 1107 48-49 0.3341986656244629 1107 50-51 0.2881082913377 1107 52-53 0.29202877904886293 1107 54-55 0.2357858348170936 1107 56-57 0.2608691495839537 1107 58-59 0.27793789662648294 1107 60-61 0.21033724604991022 1107 62-63 0.22273179628108863 1107 64-65 0.1642655630202725 1107 66-67 0.21048940519301595 1107 68-69 0.26006043930627953 1107 70-71 0.15905608932177984 1107 72-73 0.26514186681380636 1107 74-75 0.2028834746141044 1107 76-77 0.18329282322702056 1107 78-79 0.2167331050389194 1107 80-81 0.22369038559835275 1107 82-83 0.22295736069438732 1107 84-85 0.24877765816042796 1107 86-87 0.21572231499790462 1107 88-89 0.251542634711555 1107 90-91 0.19664336901679036 1107 92-93 0.2521136155620809 1107 94-95 0.34795800241703034 1107 96-97 0.3329167335294727 1107 98-99 0.375729499732099 1107 100 0.3757172914029212 1108 1 0.06965958835200325 1108 2 0.031545484841814186 1108 3 0.12942374661264466 1108 4 0.05389229126118522 1108 5 0.045077155662205826 1108 6 0.05182108803558805 1108 7 -9.669449298641553E-4 1108 8 -0.029138729595217683 1108 9 0.10957521046039886 1108 10-11 0.0649975721499203 1108 12-13 0.04712017979321814 1108 14-15 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2215 4 -0.21606584125969874 2215 5 -0.28599230477135507 2215 6 -0.2927490316295831 2215 7 -0.1840319398706427 2215 8 -0.2630680426842389 2215 9 -0.4222226103122253 2215 10-11 -0.35799706758627536 2215 12-13 -0.3268331332289094 2215 14-15 -0.40444355986201685 2215 16-17 -0.4194993293410505 2215 18-19 -0.4123731833245259 2215 20-21 -0.4415095365103454 2215 22-23 -0.373387540030933 2215 24-25 -0.396035802007745 2215 26-27 -0.4077901649868707 2215 28-29 -0.3656829029067339 2215 30-31 -0.3789179374577145 2215 32-33 -0.29513947014121555 2215 34-35 -0.3943308154354668 2215 36-37 -0.44890032253898227 2215 38-39 -0.4049819226549616 2215 40-41 -0.3414395269357229 2215 42-43 -0.24555652062884548 2215 44-45 -0.3451722232028729 2215 46-47 -0.36626448820576485 2215 48-49 -0.31175541395824524 2215 50-51 -0.2761533949780599 2215 52-53 -0.2244573699346759 2215 54-55 -0.15745522970035353 2215 56-57 -0.17102661427925625 2215 58-59 -0.13877750056690275 2215 60-61 -0.12325636434188425 2215 62-63 -0.13856996669798605 2215 64-65 -0.07003860056874345 2215 66-67 -0.014164988414783863 2215 68-69 -0.0775375647230021 2215 70-71 -0.17013047761893318 2215 72-73 -0.11690432327837641 2215 74-75 -0.11269607473779786 2215 76-77 0.004656475117769787 2215 78-79 -0.15389545663794735 2215 80-81 -0.17380833280124364 2215 82-83 -0.09289351114285438 2215 84-85 -0.17308806671270105 2215 86-87 -0.12881893714375536 2215 88-89 -0.13842793621878258 2215 90-91 -0.14979321083357178 2215 92-93 -0.18814989830449136 2215 94-95 -0.21337767254679463 2215 96-97 -0.20812941671320573 2215 98-99 -0.1807351522173235 2215 100 -0.15506679425053704 2216 1 -0.3912265664994301 2216 2 -0.3815266376888644 2216 3 -0.4986332300709293 2216 4 -0.2886719835214606 2216 5 -0.5141415379161884 2216 6 -0.3798489630362951 2216 7 -0.6586857338134848 2216 8 -0.4405982884088431 2216 9 -0.6140179124155338 2216 10-11 -0.39283857763916075 2216 12-13 -0.40261163857513793 2216 14-15 -0.7015473048083436 2216 16-17 -0.5197202718252001 2216 18-19 -0.4632520679564891 2216 20-21 -0.47464633978492543 2216 22-23 -0.5302353497467678 2216 24-25 -0.5034571328881228 2216 26-27 -0.6127590132294074 2216 28-29 -0.6233179805849005 2216 30-31 -0.743176866266225 2216 32-33 -0.7434238889782918 2216 34-35 -0.7238969660510861 2216 36-37 -0.7297869956286078 2216 38-39 -0.7202977615390509 2216 40-41 -0.6799479743497585 2216 42-43 -0.7635833693874261 2216 44-45 -0.9624985071684904 2216 46-47 -0.9620672858972483 2216 48-49 -1.0181060296932216 2216 50-51 -0.9788810734356801 2216 52-53 -0.9174896135921387 2216 54-55 -0.8210994841881813 2216 56-57 -0.915127897101712 2216 58-59 -0.9944849403625966 2216 60-61 -0.7446638804000756 2216 62-63 -0.6807180796808581 2216 64-65 -0.7568284100836493 2216 66-67 -0.6552814454694555 2216 68-69 -0.7298537837668917 2216 70-71 -0.7624793956131057 2216 72-73 -0.6777104604085338 2216 74-75 -0.6801196974239758 2216 76-77 -0.6943777438580199 2216 78-79 -0.9706709907138631 2216 80-81 -0.991922387274542 2216 82-83 -0.8952138094285615 2216 84-85 -0.9542943868357057 2216 86-87 -0.7752014805960528 2216 88-89 -1.1310950616114965 2216 90-91 -0.9357734131676132 2216 92-93 -1.0061071602895204 2216 94-95 -0.9685352348582299 2216 96-97 -1.012539341655323 2216 98-99 -1.0717515272841283 2216 100 -1.071167263977845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 257.0 3 27.0 4 33.0 5 76.0 6 248.0 7 572.0 8 1458.0 9 2529.0 10 3512.0 11 4862.0 12 6435.0 13 9249.0 14 11367.0 15 14569.0 16 18056.0 17 24555.0 18 24082.0 19 13710.0 20 10483.0 21 10507.0 22 10798.0 23 12171.0 24 14380.0 25 17851.0 26 23191.0 27 29846.0 28 39186.0 29 51398.0 30 67153.0 31 86619.0 32 111789.0 33 145308.0 34 198995.0 35 287277.0 36 466598.0 37 844465.0 38 1187218.0 39 487053.0 40 2073.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.643965029490495 20.336718799031882 14.56381004333327 23.45550612814435 2 15.361008463295372 20.45754720096152 40.109071886595046 24.07237244914806 3 17.870869414682605 25.333706293178516 30.75442292325675 26.041001368882128 4 12.449026569627877 17.29332440870821 38.272962395346745 31.98468662631717 5 12.627560286002968 37.801477185140605 35.678412700509156 13.892549828347276 6 30.664445574435206 38.25322715613086 17.223905153732726 13.858422115701199 7 26.453694330790224 33.020271908482066 22.326434519603506 18.1995992411242 8 24.844437180168434 36.37871595430771 20.157328180414428 18.619518685109437 9 24.697897808373483 16.490737168027213 21.684894843248372 37.12647018035093 10-11 23.775895315894786 27.047261811207473 28.688068932790813 20.488773940106924 12-13 24.405898221060003 24.54492417811698 29.117125003613094 21.932052597209925 14-15 21.62863279723412 26.505696177005905 26.8480823708021 25.01758865495788 16-17 20.671995181082067 29.889166302669178 27.51041520242191 21.92842331382684 18-19 20.474427205005714 28.227528358201916 30.508826154994566 20.7892182817978 20-21 21.568406166670677 27.39510853183785 30.37555360270418 20.660931698787298 22-23 21.169995632030144 27.284056721343337 30.461967058148716 21.083980588477804 24-25 21.094611964763736 27.401490583615374 30.339473342846023 21.164424108774867 26-27 20.862571085710403 27.402515405834784 30.449755091567077 21.28515841688774 28-29 20.87453432380414 27.39966742426449 30.568299784678377 21.15749846725299 30-31 21.172653955053768 27.68845424067001 30.16449467034438 20.974397133931845 32-33 20.89594076150271 27.469987526962313 30.549590790730687 21.08448092080429 34-35 21.054089514671507 27.261398888148054 30.47034955340307 21.21416204377737 36-37 21.36149434728204 27.573947485012507 29.93957595977218 21.124982207933275 38-39 21.223417823099684 27.72400279579329 29.986180233854643 21.066399147252383 40-41 21.15868361167981 27.488393434874638 30.104132136479457 21.2487908169661 42-43 21.106674723392054 27.534731656326887 29.938116990445142 21.42047662983592 44-45 21.301789033906594 27.67708850734279 29.68282349145342 21.338298967297195 46-47 21.404344390853666 27.86620125237326 29.30367103385653 21.425783322916544 48-49 21.4263881020565 27.86069851219101 29.02444523795934 21.688468147793145 50-51 21.479537530427958 27.89649034548423 28.87773226605232 21.746239858035494 52-53 21.454229711656694 27.952240232352978 28.855168194463655 21.738361861526677 54-55 21.55018377635889 28.25767888949267 28.64156440097209 21.550572933176348 56-57 21.461517570402425 28.00844401031763 28.746439945862296 21.783598473417655 58-59 21.37394137852634 27.68232063223417 29.05929506682629 21.8844429224132 60-61 21.50055596194152 28.01580019191271 28.780573631967464 21.703070214178307 62-63 21.338759223791683 28.019831041563652 28.85779526186647 21.783614472778194 64-65 21.3644556841412 27.814947525697637 28.93208795187188 21.88850883828929 66-67 21.328750826672 27.856075107573496 28.8010520079285 22.014122057826 68-69 21.644643919963613 27.958323982672688 28.65463814977559 21.742393947588106 70-71 21.640857314827393 27.837719720297667 28.700904919319537 21.820518045555403 72-73 21.493181053533036 27.96056382740737 28.751381661116042 21.79487345794355 74-75 21.549155159153106 27.98727378947341 28.584680197296596 21.87889085407689 76-77 21.575302564528307 27.998533935294095 28.434508936721002 21.991654563456592 78-79 21.649237248469245 28.07000164747774 28.51926658583766 21.76149451821536 80-81 21.594330637792638 27.91430671237277 28.565286159248387 21.926076490586205 82-83 21.473120170211622 27.982108533966084 28.674047267876812 21.870724027945478 84-85 21.539689271000103 27.90734122734936 28.521857920919587 22.031111580730954 86-87 21.600287271264975 28.02338265452328 28.45182486830452 21.92450520590722 88-89 21.427133547388664 27.905410767949345 28.508495367605065 22.158960317056927 90-91 21.359235895333736 27.781556748158774 28.595644456226864 22.263562900280622 92-93 21.44339085620846 28.04349541180648 28.395831031266557 22.117282700718505 94-95 21.31827900864675 27.776318720656786 28.568997527124214 22.33640474357225 96-97 21.31123839102755 27.8805440980569 28.505912037858177 22.30230547305737 98-99 21.328345085371307 27.913488081603234 28.448980997360273 22.309185835665186 100 21.391783153325115 27.767571821875947 28.475395286538664 22.365249738260275 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 692.0 1 490.0 2 532.5 3 765.5 4 482.0 5 282.5 6 739.0 7 2008.0 8 3420.0 9 3846.0 10 2915.0 11 1857.5 12 1767.5 13 2084.5 14 2469.0 15 2880.0 16 3284.5 17 3807.0 18 4465.5 19 5268.0 20 6455.0 21 8160.5 22 10302.5 23 13278.0 24 17927.0 25 23979.0 26 31773.5 27 41332.0 28 52657.5 29 66162.5 30 79342.5 31 90411.0 32 100130.5 33 109838.5 34 119570.5 35 127351.5 36 134044.5 37 140831.5 38 146901.0 39 149917.5 40 151179.5 41 152186.5 42 151600.5 43 150379.0 44 147452.5 45 144140.5 46 141218.0 47 136542.5 48 130789.5 49 126505.0 50 123835.5 51 119569.5 52 112128.0 53 105479.0 54 100569.5 55 95682.5 56 89373.0 57 81701.5 58 74950.5 59 68579.0 60 60486.5 61 51035.0 62 43202.0 63 36512.5 64 29692.5 65 23744.5 66 19000.5 67 14879.0 68 11301.0 69 8645.0 70 6422.0 71 4647.5 72 3610.5 73 2687.5 74 1975.5 75 1436.5 76 963.0 77 676.5 78 470.5 79 275.0 80 165.0 81 83.0 82 42.5 83 21.0 84 12.0 85 9.0 86 4.5 87 4.5 88 3.5 89 2.5 90 2.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03872681697640258 2 0.0 3 0.0 4 4.7170300823876474E-5 5 0.0 6 0.0 7 0.0 8 6.839693619462089E-4 9 0.0 10-11 0.0 12-13 0.044776408057064745 14-15 1.6509605288356766E-4 16-17 0.0 18-19 2.3585150411938237E-5 20-21 4.7170300823876474E-5 22-23 0.0 24-25 1.4151090247162943E-4 26-27 9.434060164775295E-5 28-29 4.599104330327956E-4 30-31 2.3585150411938237E-5 32-33 2.476440793253515E-4 34-35 7.075545123581471E-5 36-37 1.0613317685372206E-4 38-39 3.4198468097310444E-4 40-41 1.5330347767759855E-4 42-43 4.717030082387648E-4 44-45 3.301921057671353E-4 46-47 1.4151090247162943E-4 48-49 7.075545123581471E-4 50-51 5.424584594745794E-4 52-53 6.485916363283015E-4 54-55 4.717030082387648E-4 56-57 6.485916363283015E-4 58-59 1.886812032955059E-4 60-61 1.7688862808953678E-4 62-63 8.844431404476839E-4 64-65 5.542510346805485E-4 66-67 0.0015094496263640472 68-69 0.0014033164495103252 70-71 4.717030082387648E-4 72-73 9.787837420954367E-4 74-75 0.0010613317685372206 76-77 8.018951140059E-4 78-79 0.0033372987832892605 80-81 0.002688707146960959 82-83 0.0013561461486864487 84-85 0.0028302180494325885 86-87 0.0014386941751282326 88-89 0.0020401155106326576 90-91 0.002783047748608712 92-93 0.0025118185188714223 94-95 0.003667490889056396 96-97 0.0031132398543758473 98-99 0.0029245586510803414 100 0.0013443535734804794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4239956.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.9222478485101 #Duplication Level Percentage of deduplicated Percentage of total 1 77.81562954484772 49.74149958253602 2 13.729084211764848 17.55187847434999 3 3.948966787077537 7.572805011273148 4 1.646675909776454 4.210369025236055 5 0.8104566717989398 2.590310612260522 6 0.49540768118714934 1.9000543549740383 7 0.32288206237505157 1.4447543051883303 8 0.2203340826153349 1.126739987072923 9 0.16374217999325136 0.9420091391505567 >10 0.7919243472884802 8.854940132549068 >50 0.04085795126318218 1.7686187084798892 >100 0.013300853927917421 1.34329498391068 >500 5.164012800768787E-4 0.24823166148383383 >1k 1.106574020043319E-4 0.12288974999972097 >5k 7.377160133614942E-5 0.3457512075968632 >10k+ 3.688580066807471E-5 0.23585306393831304 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10003 0.23592225957061816 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9243 0.21799754525754514 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5421 0.12785510038311718 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.7170300823876474E-5 0.0 0.0 0.0 3 0.0 4.7170300823876474E-5 0.0 2.3585150411938237E-5 0.0 4 0.0 4.7170300823876474E-5 0.0 9.434060164775295E-5 0.0 5 0.0 9.434060164775295E-5 0.0 9.434060164775295E-5 0.0 6 0.0 9.434060164775295E-5 0.0 9.434060164775295E-5 0.0 7 0.0 9.434060164775295E-5 0.0 1.1792575205969118E-4 0.0 8 0.0 9.434060164775295E-5 0.0 1.4151090247162943E-4 0.0 9 7.075545123581471E-5 9.434060164775295E-5 0.0 2.3585150411938236E-4 0.0 10-11 1.1792575205969118E-4 1.0613317685372206E-4 0.0 2.3585150411938236E-4 0.0 12-13 1.1792575205969118E-4 1.1792575205969118E-4 0.0 2.594366545313206E-4 0.0 14-15 1.1792575205969118E-4 1.5330347767759855E-4 0.0 2.7122922973728974E-4 0.0 16-17 1.1792575205969118E-4 2.1226635370744413E-4 0.0 3.066069553551971E-4 0.0 18-19 1.1792575205969118E-4 2.1226635370744413E-4 0.0 3.301921057671353E-4 0.0 20-21 1.1792575205969118E-4 2.1226635370744413E-4 0.0 4.0094755700295E-4 0.0 22-23 1.1792575205969118E-4 2.1226635370744413E-4 0.0 5.188733090626412E-4 0.0 24-25 1.1792575205969118E-4 2.3585150411938236E-4 0.0 7.193470875641163E-4 0.0 26-27 1.1792575205969118E-4 2.3585150411938236E-4 0.0 0.0010495391933312516 0.0 28-29 1.1792575205969118E-4 2.594366545313206E-4 0.0 0.0024528556428415767 0.0 30-31 1.1792575205969118E-4 2.594366545313206E-4 0.0 0.0074293223797605445 0.0 32-33 1.1792575205969118E-4 2.594366545313206E-4 0.0 0.018372832170899887 0.0 34-35 1.297183272656603E-4 2.594366545313206E-4 0.0 0.033986201743603 0.0 36-37 1.4151090247162943E-4 2.594366545313206E-4 0.0 0.060236474152090255 0.0 38-39 1.4151090247162943E-4 2.594366545313206E-4 0.0 0.09305521095030231 0.0 40-41 1.4151090247162943E-4 2.594366545313206E-4 0.0 0.12809095188723657 0.0 42-43 1.6509605288356766E-4 2.594366545313206E-4 0.0 0.16396396566379462 0.0 44-45 1.6509605288356766E-4 2.594366545313206E-4 0.0 0.2076200790762923 0.0 46-47 1.6509605288356766E-4 2.594366545313206E-4 0.0 0.2580215455066043 0.0 48-49 1.6509605288356766E-4 2.594366545313206E-4 0.0 0.3094843437054535 0.0 50-51 1.6509605288356766E-4 2.8302180494325886E-4 0.0 0.36256272470752055 0.0 52-53 1.886812032955059E-4 2.8302180494325886E-4 0.0 0.4146269442418742 0.0 54-55 1.886812032955059E-4 2.8302180494325886E-4 0.0 0.47242235532632887 0.0 56-57 1.886812032955059E-4 3.183995305611662E-4 0.0 0.5395456933987051 0.0 58-59 1.886812032955059E-4 3.301921057671353E-4 0.0 0.6127068299765375 0.0 60-61 1.886812032955059E-4 3.5377725617907356E-4 0.0 0.685431641271749 0.0 62-63 1.886812032955059E-4 3.5377725617907356E-4 0.0 0.7607626116874797 0.0 64-65 2.1226635370744413E-4 3.5377725617907356E-4 0.0 0.8391478590815564 0.0 66-67 2.1226635370744413E-4 3.5377725617907356E-4 0.0 0.9303162579989037 0.0 68-69 2.1226635370744413E-4 3.773624065910118E-4 0.0 1.0303880511967578 0.0 70-71 2.1226635370744413E-4 3.773624065910118E-4 0.0 1.135695747786062 0.0 72-73 2.1226635370744413E-4 3.773624065910118E-4 0.0 1.2443879134594793 0.0 74-75 2.1226635370744413E-4 3.773624065910118E-4 0.0 1.3545895287592606 0.0 76-77 2.1226635370744413E-4 3.773624065910118E-4 0.0 1.475463424620444 0.0 78-79 2.1226635370744413E-4 3.773624065910118E-4 0.0 1.6080709328115668 0.0 80-81 2.1226635370744413E-4 4.0094755700295E-4 0.0 1.7534497999507543 0.0 82-83 2.1226635370744413E-4 4.2453270741488826E-4 0.0 1.8990291408684428 0.0 84-85 2.1226635370744413E-4 4.2453270741488826E-4 0.0 2.044596689210926 0.0 86-87 2.1226635370744413E-4 4.717030082387647E-4 0.0 2.1988317803297956 2.3585150411938237E-5 88 2.1226635370744413E-4 4.717030082387647E-4 0.0 2.3229722195230327 2.3585150411938237E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6715 0.0 58.8783 1 GTATCAA 12130 0.0 39.76416 1 ATCAACG 15250 0.0 31.279371 3 TCAACGC 15290 0.0 31.259018 4 CAACGCA 15850 0.0 30.154598 5 TATCAAC 16045 0.0 29.81741 2 AACGCAG 16520 0.0 29.014177 6 GTGGTAT 3195 0.0 28.692541 1 TGGTATC 3040 0.0 27.362864 2 ACGCAGA 18910 0.0 25.223055 7 CGCAGAG 19115 0.0 24.90333 8 GTACATG 11345 0.0 23.495455 1 TACATGG 11290 0.0 22.353327 2 GCAGAGT 21230 0.0 22.001148 9 GAGTACT 14950 0.0 21.307114 12-13 ACATGGG 11755 0.0 20.669489 3 AGAGTAC 19570 0.0 20.28606 10-11 CAGAGTA 20820 0.0 20.141266 10-11 GTACTTT 16930 0.0 18.75125 14-15 AGTACTT 15955 0.0 18.521023 12-13 >>END_MODULE