FastQCFastQC Report
Wed 25 May 2016
SRR2049342_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049342_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4390376
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43350.047.174711
GTATCAA77550.039.767761
TCAACGC83500.035.4657174
ATCAACG84400.035.0318343
CAACGCA89800.033.0299345
AACGCAG92700.031.9933536
TATCAAC96650.031.1266782
ACGCAGA107050.027.5287237
CGCAGAG111200.026.3322778
GTACATG116550.024.4841751
TACATGG117850.022.8948062
GCAGAGT127500.022.007439
ACATGGG124150.021.2407973
CATGGGG93250.018.9536864
CAGAGTA120700.018.63287410-11
AGAGTAC111100.018.42380310-11
GTGGTAT27150.017.8351381
GTCTCGC10550.017.8244691
GAGTACT61050.017.47613512-13
CCGGTCG3703.1650416E-916.5137929
Produced by FastQC (version 0.11.5)