##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049342_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4390376
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.534038086942896	31.0	31.0	34.0	27.0	34.0
2	30.778819399522956	31.0	31.0	34.0	27.0	34.0
3	30.959333779156957	31.0	31.0	34.0	27.0	34.0
4	34.56990881874354	37.0	35.0	37.0	32.0	37.0
5	34.60583945429731	37.0	35.0	37.0	32.0	37.0
6	34.60390157927248	37.0	35.0	37.0	32.0	37.0
7	34.508277423163754	37.0	35.0	37.0	31.0	37.0
8	34.4705874849899	37.0	35.0	37.0	31.0	37.0
9	36.060955371476155	39.0	37.0	39.0	31.0	39.0
10-11	36.01819992182902	39.0	37.0	39.0	31.0	39.0
12-13	35.94732307665676	39.0	37.0	39.0	30.0	39.0
14-15	37.12982054384408	40.0	37.0	41.0	31.0	41.0
16-17	37.09214131090367	40.0	37.0	41.0	31.0	41.0
18-19	37.12070594864768	40.0	37.0	41.0	31.0	41.0
20-21	37.19138748025226	40.0	37.5	41.0	31.0	41.0
22-23	37.143416190321744	40.0	37.0	41.0	31.0	41.0
24-25	37.141634224494666	40.0	37.0	41.0	31.0	41.0
26-27	37.12353213027768	40.0	37.0	41.0	31.0	41.0
28-29	37.0642906211222	40.0	37.0	41.0	31.0	41.0
30-31	36.99741104178777	40.0	37.0	41.0	30.5	41.0
32-33	36.93363381632917	40.0	37.0	41.0	30.0	41.0
34-35	36.83248575976181	40.0	37.0	41.0	30.0	41.0
36-37	36.74967964019483	40.0	37.0	41.0	30.0	41.0
38-39	36.637117185407355	40.0	37.0	41.0	30.0	41.0
40-41	36.52623146627988	40.0	36.5	41.0	30.0	41.0
42-43	36.3820079419166	40.0	36.0	41.0	30.0	41.0
44-45	36.25304757496852	40.0	36.0	41.0	29.0	41.0
46-47	36.124048486963304	40.0	36.0	41.0	29.0	41.0
48-49	35.98940705761875	40.0	35.0	41.0	28.5	41.0
50-51	35.850080494244686	39.0	35.0	41.0	28.0	41.0
52-53	35.70576267271869	39.0	35.0	41.0	28.0	41.0
54-55	35.56615834270231	39.0	35.0	41.0	28.0	41.0
56-57	35.437637687523804	39.0	35.0	41.0	28.0	41.0
58-59	35.23550055849431	39.0	35.0	41.0	27.0	41.0
60-61	35.0215453072812	38.5	34.0	40.0	26.5	41.0
62-63	34.8147591459137	38.0	34.0	40.0	26.0	41.0
64-65	34.57410697853669	38.0	34.0	40.0	26.0	41.0
66-67	34.31617087010315	37.0	34.0	40.0	26.0	41.0
68-69	34.03254618738805	37.0	34.0	40.0	26.0	41.0
70-71	33.75020601880112	36.0	34.0	39.0	25.5	41.0
72-73	33.41144346179006	36.0	33.0	39.0	25.0	41.0
74-75	33.06845438750577	35.5	33.0	39.0	24.0	40.0
76-77	32.738128009992764	35.0	33.0	38.0	24.0	40.0
78-79	32.426943387081195	35.0	33.0	37.0	23.0	39.0
80-81	32.082071216679395	35.0	32.5	37.0	22.5	39.0
82-83	31.77670158546785	35.0	32.0	36.0	22.0	39.0
84-85	31.491351538000387	35.0	32.0	36.0	20.5	37.0
86-87	31.18208577124146	35.0	32.0	35.5	20.0	37.0
88-89	30.937877302536275	35.0	32.0	35.0	18.5	37.0
90-91	30.743913049816236	35.0	32.0	35.0	18.0	36.0
92-93	30.51583634294648	35.0	32.0	35.0	13.5	36.0
94-95	30.3207311856661	35.0	32.0	35.0	4.5	36.0
96-97	30.076018090477902	34.5	31.5	35.0	2.0	35.5
98-99	29.879365685308045	34.0	31.0	35.0	2.0	35.0
100	29.765655834488893	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.09396888479585286
1101	2	0.1669250675782834
1101	3	0.20174961428200788
1101	4	0.2642184440281241
1101	5	0.28169961453277637
1101	6	0.3696138697353888
1101	7	0.35056346120568094
1101	8	0.316078179799959
1101	9	0.44165135882077067
1101	10-11	0.4656696534406066
1101	12-13	0.49165682810945555
1101	14-15	0.5253648275830329
1101	16-17	0.5874906995752838
1101	18-19	0.5477812221813281
1101	20-21	0.561103668420742
1101	22-23	0.5540876119513953
1101	24-25	0.5626510127520135
1101	26-27	0.5028513101717849
1101	28-29	0.4683675636735316
1101	30-31	0.4624748348669314
1101	32-33	0.46796085112711694
1101	34-35	0.5111884702479088
1101	36-37	0.4969893401420862
1101	38-39	0.5258264100519696
1101	40-41	0.5772845333578971
1101	42-43	0.5289538505613365
1101	44-45	0.458950067425306
1101	46-47	0.46820435439121155
1101	48-49	0.4237625587332161
1101	50-51	0.40989101722310295
1101	52-53	0.4529700372765859
1101	54-55	0.41899596761989244
1101	56-57	0.444065649204191
1101	58-59	0.5260648780968253
1101	60-61	0.4991893966521985
1101	62-63	0.41937500747818746
1101	64-65	0.41975561484962043
1101	66-67	0.4848566279862041
1101	68-69	0.463659111572035
1101	70-71	0.46019792804591475
1101	72-73	0.5770197968922943
1101	74-75	0.5236327759457993
1101	76-77	0.5460292217824403
1101	78-79	0.4545366535697397
1101	80-81	0.4740703865453284
1101	82-83	0.566372979725756
1101	84-85	0.46915266510422526
1101	86-87	0.5251602651125751
1101	88-89	0.5577936049814873
1101	90-91	0.5611605532689765
1101	92-93	0.5454738373036427
1101	94-95	0.5216683621100344
1101	96-97	0.5653212809268489
1101	98-99	0.6201943467447713
1101	100	0.624364520712664
1102	1	0.11271718269785325
1102	2	0.14133748819673286
1102	3	0.2551020410900833
1102	4	0.24978245356788875
1102	5	0.2154081637421541
1102	6	0.34365840258194424
1102	7	0.42298842764270717
1102	8	0.37566685908139164
1102	9	0.45634727052066637
1102	10-11	0.44286557247186664
1102	12-13	0.5436947904911591
1102	14-15	0.6081967364489174
1102	16-17	0.5749179749027462
1102	18-19	0.5495393073545642
1102	20-21	0.5431758721755244
1102	22-23	0.5118743351861994
1102	24-25	0.5830566629060385
1102	26-27	0.513480515074761
1102	28-29	0.5051240584298426
1102	30-31	0.5267294132050822
1102	32-33	0.5897416254011034
1102	34-35	0.6321946371945657
1102	36-37	0.5990780502152475
1102	38-39	0.548844297990037
1102	40-41	0.6600296144535207
1102	42-43	0.6236925520030354
1102	44-45	0.4908859713754694
1102	46-47	0.4602408084672547
1102	48-49	0.4821918807815919
1102	50-51	0.5442517253427894
1102	52-53	0.5493258662278819
1102	54-55	0.5602622816121752
1102	56-57	0.47475123815013376
1102	58-59	0.5554452645710697
1102	60-61	0.5601364063458192
1102	62-63	0.5420824904142023
1102	64-65	0.4957263947756019
1102	66-67	0.5867929190322982
1102	68-69	0.6080572858868081
1102	70-71	0.6563219442015011
1102	72-73	0.5702518740080649
1102	74-75	0.529027265811024
1102	76-77	0.5348265088263986
1102	78-79	0.5814572017367041
1102	80-81	0.5815706929962801
1102	82-83	0.5579361699124519
1102	84-85	0.5444365696745166
1102	86-87	0.5908261063004012
1102	88-89	0.5653679533064455
1102	90-91	0.6082319818543667
1102	92-93	0.5498626352944989
1102	94-95	0.4938162225936189
1102	96-97	0.4731221145907476
1102	98-99	0.5412571509107842
1102	100	0.547871674460719
1103	1	0.058186262704879255
1103	2	0.09991774604886672
1103	3	0.16749723921990523
1103	4	0.09655563283921964
1103	5	0.2627492992270817
1103	6	0.2915970140524138
1103	7	0.2498589491612222
1103	8	0.26691708321122576
1103	9	0.3040298515268418
1103	10-11	0.27166536400606844
1103	12-13	0.3106746711467281
1103	14-15	0.3440911926958634
1103	16-17	0.32353281912547516
1103	18-19	0.2913411055843653
1103	20-21	0.2894154649887568
1103	22-23	0.2838330114178973
1103	24-25	0.3493650963809145
1103	26-27	0.26723768973438666
1103	28-29	0.2925900143126938
1103	30-31	0.32598564807005204
1103	32-33	0.3548745283097361
1103	34-35	0.35802405356381684
1103	36-37	0.3497200825916664
1103	38-39	0.38087801227013784
1103	40-41	0.34861925641547487
1103	42-43	0.3634228490224203
1103	44-45	0.24542272184545055
1103	46-47	0.23760288643399718
1103	48-49	0.3078433578628079
1103	50-51	0.20625992675322635
1103	52-53	0.22802770861090949
1103	54-55	0.20908728304116408
1103	56-57	0.2433700577848512
1103	58-59	0.30296430594522405
1103	60-61	0.24378308487765565
1103	62-63	0.2496740362988632
1103	64-65	0.29399221194844927
1103	66-67	0.3124206424066003
1103	68-69	0.3509146414057298
1103	70-71	0.29164626615566647
1103	72-73	0.33103140973987166
1103	74-75	0.33754820977193845
1103	76-77	0.33922664864333996
1103	78-79	0.27207298217562936
1103	80-81	0.23881930234163207
1103	82-83	0.18747812105343087
1103	84-85	0.31262295828754816
1103	86-87	0.3183380709449999
1103	88-89	0.2474143996879583
1103	90-91	0.3117698194077363
1103	92-93	0.3236968333228809
1103	94-95	0.35244421210868637
1103	96-97	0.3170278394989161
1103	98-99	0.32431339106231505
1103	100	0.2863920759296157
1104	1	-0.0040360951743565465
1104	2	0.007679955642650782
1104	3	0.10797855647417975
1104	4	0.22089393491902598
1104	5	0.24100645498044315
1104	6	0.2315193242050455
1104	7	0.25978464919766253
1104	8	0.2716052740214536
1104	9	0.3899182230692091
1104	10-11	0.4158138603405561
1104	12-13	0.32194027709886797
1104	14-15	0.43056049228019333
1104	16-17	0.42705107223926575
1104	18-19	0.3702998354832303
1104	20-21	0.42959862970658946
1104	22-23	0.34833369031959904
1104	24-25	0.42139227695951575
1104	26-27	0.3367397421633527
1104	28-29	0.33142416251305207
1104	30-31	0.35411635375784556
1104	32-33	0.3732807071476074
1104	34-35	0.395924307273269
1104	36-37	0.34354886386137906
1104	38-39	0.3690519308833231
1104	40-41	0.32674844752352783
1104	42-43	0.363600505757141
1104	44-45	0.3430888007164441
1104	46-47	0.2654887736031313
1104	48-49	0.26001027056197046
1104	50-51	0.3110615376186132
1104	52-53	0.2213188256354357
1104	54-55	0.2813908917644099
1104	56-57	0.22584443942010068
1104	58-59	0.30749828710246163
1104	60-61	0.26807242325372727
1104	62-63	0.2669483616056212
1104	64-65	0.30590080902676675
1104	66-67	0.35108592074705314
1104	68-69	0.30469007247130975
1104	70-71	0.302962593186507
1104	72-73	0.33802834034575824
1104	74-75	0.3378159319734948
1104	76-77	0.34180967767331794
1104	78-79	0.33717625570272247
1104	80-81	0.32466680335443243
1104	82-83	0.2577878607029547
1104	84-85	0.3428931523757406
1104	86-87	0.32854756391457585
1104	88-89	0.336346429049069
1104	90-91	0.3693305089646728
1104	92-93	0.3901572974733547
1104	94-95	0.4239499957221824
1104	96-97	0.35869465646738874
1104	98-99	0.34625585923070545
1104	100	0.3231310400773175
1105	1	0.17476453045621199
1105	2	0.21372244638541815
1105	3	0.3070750754699709
1105	4	0.34741290401650815
1105	5	0.330658060961305
1105	6	0.3864988568682648
1105	7	0.2749345265092984
1105	8	0.4023922900518997
1105	9	0.4428759111542888
1105	10-11	0.4176011601823717
1105	12-13	0.41783889149441933
1105	14-15	0.42154318690450765
1105	16-17	0.46680035514849294
1105	18-19	0.3916063397457421
1105	20-21	0.47013823607179006
1105	22-23	0.4561629026051577
1105	24-25	0.40202274077312694
1105	26-27	0.4002922241337714
1105	28-29	0.469417028972245
1105	30-31	0.5069452924634916
1105	32-33	0.5020846579975284
1105	34-35	0.5334092158931227
1105	36-37	0.48162637327875046
1105	38-39	0.5394026180804943
1105	40-41	0.5469705855216915
1105	42-43	0.4799257758227853
1105	44-45	0.44531784316003353
1105	46-47	0.4402833439981393
1105	48-49	0.4797642310205461
1105	50-51	0.4974342326521821
1105	52-53	0.4887975019712627
1105	54-55	0.4823250003742956
1105	56-57	0.42074765663099356
1105	58-59	0.39513047291261216
1105	60-61	0.3432468137710458
1105	62-63	0.40357957854248383
1105	64-65	0.426412963982429
1105	66-67	0.4620237571613828
1105	68-69	0.4390228733247028
1105	70-71	0.4090729784582052
1105	72-73	0.35302606940999937
1105	74-75	0.45524519710669153
1105	76-77	0.40589998885157286
1105	78-79	0.34764971800563416
1105	80-81	0.40862181925320584
1105	82-83	0.40743135047951284
1105	84-85	0.44011430326196077
1105	86-87	0.5236318220814944
1105	88-89	0.47178981980304613
1105	90-91	0.5337672546180876
1105	92-93	0.4573088567454455
1105	94-95	0.4541057538899871
1105	96-97	0.4179314819260682
1105	98-99	0.4353090814238634
1105	100	0.4774480200041147
1106	1	0.19600356603081437
1106	2	0.12426351739514985
1106	3	0.23844320952677123
1106	4	0.28690318038663065
1106	5	0.29152667778828345
1106	6	0.3106422166526741
1106	7	0.3780757360028204
1106	8	0.37123278920287817
1106	9	0.4410587030579265
1106	10-11	0.42778229002124135
1106	12-13	0.44829514793553926
1106	14-15	0.5082126150360295
1106	16-17	0.5283664471257907
1106	18-19	0.5231010733535157
1106	20-21	0.5330639173384526
1106	22-23	0.4910418675787369
1106	24-25	0.5951482922423068
1106	26-27	0.5457617849798311
1106	28-29	0.5570446400339648
1106	30-31	0.5218904677705893
1106	32-33	0.5392887958300605
1106	34-35	0.5989917158982578
1106	36-37	0.5810438511257203
1106	38-39	0.565237569560928
1106	40-41	0.6359251690401777
1106	42-43	0.5994219554182578
1106	44-45	0.5488959172586902
1106	46-47	0.5462709366371215
1106	48-49	0.5144081473654509
1106	50-51	0.5467799783162945
1106	52-53	0.5706659030452954
1106	54-55	0.5461031056528824
1106	56-57	0.5012700812706044
1106	58-59	0.4956622817790475
1106	60-61	0.552337996886962
1106	62-63	0.5025864883628302
1106	64-65	0.5423779906133888
1106	66-67	0.5772732229918773
1106	68-69	0.5501349279723158
1106	70-71	0.5408259889788951
1106	72-73	0.5900703966470431
1106	74-75	0.5741043923255162
1106	76-77	0.6218493498954913
1106	78-79	0.48586490202171717
1106	80-81	0.5421230768757326
1106	82-83	0.5500593791101664
1106	84-85	0.5057225056336918
1106	86-87	0.5399086263060582
1106	88-89	0.5588121882086021
1106	90-91	0.6119107788371387
1106	92-93	0.5558343252626408
1106	94-95	0.5808138079572203
1106	96-97	0.5329532673557118
1106	98-99	0.5789652263260052
1106	100	0.5423659533955494
1107	1	0.05751313914610279
1107	2	0.026464791246752384
1107	3	0.07086421124278885
1107	4	0.10668804594256187
1107	5	0.09167712832804398
1107	6	0.09891495814266449
1107	7	0.17706768320284283
1107	8	0.24726011300354855
1107	9	0.3064848400042379
1107	10-11	0.18817919785885806
1107	12-13	0.23289888958619542
1107	14-15	0.202611077799844
1107	16-17	0.28003831325942485
1107	18-19	0.2179813324993347
1107	20-21	0.21457199278506778
1107	22-23	0.23084991174736302
1107	24-25	0.2505780035105829
1107	26-27	0.22862691703105043
1107	28-29	0.2941856054811893
1107	30-31	0.29967006392561757
1107	32-33	0.32459636698321503
1107	34-35	0.2970531215959724
1107	36-37	0.2523587529504496
1107	38-39	0.2748381599115888
1107	40-41	0.26593815025970713
1107	42-43	0.2992793777873217
1107	44-45	0.2185080595273874
1107	46-47	0.236062540633867
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2215	28-29	-0.5599085471258292
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2215	100	-0.5110650293276642
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2216	2	-1.0111105976792452
2216	3	-1.2268354321759745
2216	4	-1.4071567596996317
2216	5	-1.2895037117415882
2216	6	-2.691197061879528
2216	7	-2.654924553397752
2216	8	-3.4139757953319325
2216	9	-2.7911863867035933
2216	10-11	-2.15210536862935
2216	12-13	-1.907248622562804
2216	14-15	-2.137812408439167
2216	16-17	-2.507729821171182
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2216	24-25	-2.330423469425007
2216	26-27	-2.30082390578805
2216	28-29	-2.7961815897023357
2216	30-31	-2.394833200791247
2216	32-33	-2.545402932871106
2216	34-35	-3.5456601368623097
2216	36-37	-2.573506880217927
2216	38-39	-2.403777385820746
2216	40-41	-2.91023313101347
2216	42-43	-2.7484823501459275
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2216	52-53	-3.2366455162566155
2216	54-55	-2.633488236453587
2216	56-57	-2.5927286853807345
2216	58-59	-3.539034891193161
2216	60-61	-4.278045687342853
2216	62-63	-3.406474847268143
2216	64-65	-3.3824824652069196
2216	66-67	-3.0520546946125293
2216	68-69	-3.380104889594172
2216	70-71	-3.1789435485441793
2216	72-73	-3.6184294689442993
2216	74-75	-4.054309011243376
2216	76-77	-4.757870853681133
2216	78-79	-3.6934809538897326
2216	80-81	-3.556994323426185
2216	82-83	-3.7981882242785012
2216	84-85	-3.827442707100431
2216	86-87	-3.529602848652967
2216	88-89	-3.481024376627211
2216	90-91	-3.6173764765463083
2216	92-93	-3.7028131634361223
2216	94-95	-3.599658054938537
2216	96-97	-3.8494035071493755
2216	98-99	-3.6548042902021436
2216	100	-3.2656239517526906
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77305.0
3	13629.0
4	8835.0
5	7090.0
6	6412.0
7	6609.0
8	7100.0
9	8263.0
10	9254.0
11	10457.0
12	11877.0
13	13173.0
14	15003.0
15	15952.0
16	16578.0
17	17616.0
18	16547.0
19	14498.0
20	14322.0
21	14960.0
22	16716.0
23	19723.0
24	23718.0
25	29573.0
26	36016.0
27	42300.0
28	51124.0
29	63521.0
30	76608.0
31	91371.0
32	116010.0
33	152051.0
34	208209.0
35	299570.0
36	484742.0
37	843022.0
38	1118612.0
39	411076.0
40	934.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.346442938158916	20.261955173197777	14.389332436669713	23.002269451973596
2	15.130117318183704	20.104370937465394	40.81782299414526	23.947688750205646
3	18.116395451589394	25.42206692822777	30.37494172297644	26.086595897206394
4	12.485544259347666	16.948747776806783	39.232065481799154	31.333642482046397
5	12.970607940277013	37.99619894901396	35.6758259537949	13.35736715691413
6	30.670783966412607	38.17090986900889	17.747161608572586	13.411144556005919
7	26.655052780895193	32.726160296689585	22.708830616906976	17.90995630550824
8	24.564891139042054	36.650136882292166	20.67934360303725	18.105628375628527
9	24.493259101875932	17.249926853211488	21.930478193142157	36.32633585177042
10-11	23.54392258605825	27.296649799124218	29.097416523552898	20.062011091264633
12-13	23.976498780412218	24.759192939151866	29.216544738271722	22.047763542164194
14-15	21.427819451726844	27.019496480862305	26.401872832512197	25.15081123489865
16-17	20.925601116071554	30.398981909409763	27.027229024240608	21.64818795027808
18-19	20.842058849667424	29.292133704913105	29.093795787597955	20.77201165782152
20-21	21.93607904844903	28.502561867116967	28.686451641717547	20.874907442716456
22-23	21.62104516940106	28.358269834211264	28.701306943128962	21.31937805325871
24-25	21.532449617052226	28.45041503098491	28.626143534093423	21.390991817869438
26-27	21.218062591487367	28.399182971090447	28.74705144073806	21.635702996684124
28-29	21.2566801357435	28.460255057967615	28.844161345458463	21.438903460830417
30-31	21.55264630321685	28.771007291562583	28.439065111367256	21.23728129385331
32-33	21.2518711975767	28.546679194675527	28.81759909528544	21.383850512462327
34-35	21.38596649733689	28.290540224147854	28.845446809460878	21.478046469054373
36-37	21.62138945217436	28.663280004165255	28.355989884444927	21.359340659215462
38-39	21.528538970023863	28.80555393205853	28.40808768114382	21.25781941677379
40-41	21.44596218265373	28.507895760422237	28.58435485771043	21.461787199213603
42-43	21.374356632642368	28.51916362015646	28.539055941857	21.567423805344177
44-45	21.468154600945454	28.615247479338603	28.41266946014171	21.50392845957423
46-47	21.470511702262435	28.690753850094016	28.296766896255036	21.541967551388513
48-49	21.43939138463277	28.55373872449254	28.32236125554718	21.684508635327507
50-51	21.503319115658982	28.503391347125973	28.262874233623	21.730415303592046
52-53	21.470762314314733	28.476710053279575	28.334194457625568	21.718333174780128
54-55	21.584356201986544	28.78091890303653	28.110705762566113	21.524019132410814
56-57	21.482786332603514	28.589113394676524	28.17718526295978	21.75091500976018
58-59	21.397992987921516	28.27155706012218	28.467138315948205	21.863311636008095
60-61	21.61830139077582	28.566130920338708	28.14694430915497	21.668623379730505
62-63	21.482339910454602	28.65196451770088	28.155943294217558	21.70975227762697
64-65	21.531049948232617	28.409948451353245	28.26107165772282	21.797929942691322
66-67	21.420210886178108	28.492066078003326	28.155540005919594	21.932183029898972
68-69	21.749258561856085	28.578190963842243	28.101542391851197	21.571008082450476
70-71	21.713069475815995	28.4378063971917	28.153542717707058	21.695581409285243
72-73	21.556503589380316	28.567566511451346	28.255525736300598	21.62040416286774
74-75	21.68703276271808	28.60142680395458	28.099390953853305	21.612149479474027
76-77	21.69336582050326	28.556821616431517	27.997501372524823	21.752311190540397
78-79	21.73178315610333	28.623388891945922	28.08377528331566	21.561052668635092
80-81	21.647343533432174	28.53951928792984	28.104375679716075	21.70876149892191
82-83	21.549640389892524	28.586255102560454	28.23759888836496	21.626505619182062
84-85	21.624004848727893	28.489022006115594	28.07619657399095	21.81077657116556
86-87	21.7425596341762	28.628232518858603	27.991643234753532	21.637564612211662
88-89	21.583231406103604	28.48283521146786	28.0837923856258	21.850140996802736
90-91	21.550312015590865	28.43020623539011	28.154374290653568	21.86510745836545
92-93	21.70604871215425	28.632027601154025	27.951716664365357	21.710207022326365
94-95	21.574849689512888	28.440450798165944	28.103828676463383	21.88087083585778
96-97	21.612021384956552	28.465363786609622	28.072891706769532	21.849723121664294
98-99	21.592011709247682	28.543329774033023	28.009070293751602	21.855588222967693
100	21.654409553988085	28.482139115191956	27.984641862109306	21.878809468710653
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2061.0
1	1621.0
2	1368.0
3	1548.0
4	1427.5
5	1158.5
6	908.0
7	806.0
8	811.5
9	826.5
10	777.0
11	706.0
12	737.5
13	843.5
14	985.0
15	1178.5
16	1382.5
17	1643.0
18	2185.5
19	3047.5
20	4230.5
21	5986.5
22	8590.5
23	12494.5
24	18069.5
25	25149.5
26	34292.0
27	45108.0
28	57676.5
29	72634.5
30	86995.5
31	99169.0
32	108835.0
33	118126.0
34	127696.5
35	135115.0
36	141245.0
37	148066.5
38	154317.5
39	157236.5
40	158504.5
41	159210.5
42	157478.5
43	155192.5
44	152416.5
45	148182.0
46	144603.0
47	139448.5
48	133540.5
49	129666.5
50	126081.0
51	120666.0
52	113076.0
53	106750.5
54	101555.0
55	96308.0
56	89675.0
57	81855.0
58	75265.5
59	69072.0
60	61434.5
61	52648.5
62	45342.5
63	38654.5
64	31946.5
65	26089.5
66	20955.5
67	16458.0
68	12841.0
69	10009.0
70	7641.5
71	5745.0
72	4303.0
73	3125.5
74	2356.5
75	1755.0
76	1244.5
77	890.5
78	626.0
79	452.0
80	327.5
81	214.5
82	141.0
83	110.0
84	90.0
85	76.5
86	61.5
87	44.5
88	34.0
89	27.5
90	22.0
91	13.0
92	11.5
93	13.0
94	9.0
95	9.0
96	11.5
97	8.0
98	11.0
99	16.5
100	20.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017538361179088077
2	0.04095321220779268
3	0.04222872938445363
4	0.04512142012438115
5	0.04819632760383165
6	0.046989141704491824
7	0.044438107351169924
8	0.04405089677968356
9	0.04418755933432581
10-11	0.04481392937643609
12-13	0.04573640162027125
14-15	0.044255890611646934
16-17	0.04028128798080164
18-19	0.04341313819135309
20-21	0.042763991056802425
22-23	0.04394839986370188
24-25	0.04072544128338894
26-27	0.04081654965315044
28-29	0.041112651854875296
30-31	0.04123792586329736
32-33	0.04121514877085698
34-35	0.04098737784645324
36-37	0.038481897678012085
38-39	0.038755222787296574
40-41	0.039973797232856594
42-43	0.03981435758577397
44-45	0.038641337325094706
46-47	0.03762775671149806
48-49	0.03780997345102105
50-51	0.03888049679571864
52-53	0.03948408974538855
54-55	0.03866411441753508
56-57	0.03701277521560796
58-59	0.03787830472834217
60-61	0.037320265963553007
62-63	0.03763914525771824
64-65	0.03443896376984568
66-67	0.03560059548430476
68-69	0.0366255646441216
70-71	0.03801496728298442
72-73	0.034769231610231106
74-75	0.035213384912818405
76-77	0.03568031530784607
78-79	0.03615863424909393
80-81	0.03632946244239674
82-83	0.03474645451779073
84-85	0.03635223953483711
86-87	0.036363628081057295
88-89	0.03698999812316758
90-91	0.03765053380393843
92-93	0.035851143501148874
94-95	0.037058329400488706
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4390376.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.9166036646998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.95910612234871	52.83675210108181
2	13.072348705962655	17.495143546473088
3	3.7754687027337743	7.579246284879429
4	1.5694267633489176	4.200828348151685
5	0.7956363790956732	2.6620642120581004
6	0.441227685679094	1.7715274901088405
7	0.2918832309159062	1.3672284135700221
8	0.20534587620944936	1.0992838889990586
9	0.15396963748154077	0.9272812686974795
>10	0.7022334068698639	8.027780246053652
>50	0.02603432439518249	1.169326835938611
>100	0.007182870109016724	0.7473436321962201
>500	3.4073740350742853E-5	0.013042166156588418
>1k	1.0222110991546723E-4	0.10315156563549066
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.2777092440374127E-5	0.0
5	0.0	0.0	0.0	2.2777092440374127E-5	0.0
6	0.0	0.0	0.0	2.2777092440374127E-5	0.0
7	0.0	0.0	0.0	2.2777092440374127E-5	0.0
8	0.0	0.0	0.0	2.2777092440374127E-5	0.0
9	0.0	0.0	0.0	4.5554184880748255E-5	0.0
10-11	0.0	0.0	0.0	6.833127732112239E-5	0.0
12-13	0.0	0.0	0.0	6.833127732112239E-5	0.0
14-15	0.0	0.0	0.0	7.971982354130945E-5	0.0
16-17	0.0	0.0	0.0	9.110836976149651E-5	0.0
18-19	0.0	0.0	0.0	9.110836976149651E-5	0.0
20-21	0.0	2.2777092440374127E-5	0.0	2.0499383196336714E-4	0.0
22-23	0.0	2.2777092440374127E-5	0.0	3.3026784038542485E-4	0.0
24-25	0.0	2.2777092440374127E-5	0.0	5.808158572295403E-4	0.0
26-27	0.0	2.2777092440374127E-5	0.0	9.566378824957134E-4	0.0
28-29	0.0	2.2777092440374127E-5	0.0	0.002141046689395168	0.0
30-31	0.0	2.2777092440374127E-5	0.0	0.0055234449167907256	0.0
32-33	0.0	2.2777092440374127E-5	0.0	0.0137345867415456	0.0
34-35	0.0	2.2777092440374127E-5	0.0	0.02736667656710951	0.0
36-37	0.0	2.2777092440374127E-5	0.0	0.04779772848612511	0.0
38-39	0.0	2.2777092440374127E-5	0.0	0.07507329668347312	0.0
40-41	0.0	2.2777092440374127E-5	0.0	0.10486573359548249	0.0
42-43	0.0	2.2777092440374127E-5	0.0	0.1341001317427027	0.0
44-45	0.0	2.2777092440374127E-5	0.0	0.16952989903370463	0.0
46-47	0.0	2.2777092440374127E-5	0.0	0.21369468127559005	0.0
48-49	0.0	2.2777092440374127E-5	0.0	0.2597727392824669	0.0
50-51	0.0	2.2777092440374127E-5	0.0	0.3061924536759494	0.0
52-53	0.0	2.2777092440374127E-5	0.0	0.3498105856992658	0.0
54-55	0.0	2.2777092440374127E-5	0.0	0.39863328334520776	0.0
56-57	0.0	2.2777092440374127E-5	0.0	0.4588331386651166	0.0
58-59	0.0	2.2777092440374127E-5	0.0	0.5230303737083111	0.0
60-61	0.0	2.2777092440374127E-5	0.0	0.5880134184406984	0.0
62-63	0.0	2.2777092440374127E-5	0.0	0.6512198499627366	0.0
64-65	0.0	2.2777092440374127E-5	0.0	0.7187311519560056	0.0
66-67	0.0	2.2777092440374127E-5	0.0	0.7981548732955901	0.0
68-69	0.0	2.2777092440374127E-5	0.0	0.8832842562914884	0.0
70-71	0.0	2.2777092440374127E-5	0.0	0.9730601661452232	0.0
72-73	0.0	2.2777092440374127E-5	0.0	1.0676762081425373	0.0
74-75	0.0	2.2777092440374127E-5	0.0	1.1646269021149895	0.0
76-77	0.0	2.2777092440374127E-5	0.0	1.269845680643298	0.0
78-79	0.0	2.2777092440374127E-5	0.0	1.385382482047096	0.0
80-81	0.0	2.2777092440374127E-5	0.0	1.5094834702084743	0.0
82-83	0.0	2.2777092440374127E-5	0.0	1.6373996213536153	0.0
84-85	0.0	3.416563866056119E-5	0.0	1.764017478229655	0.0
86-87	0.0	4.5554184880748255E-5	0.0	1.9012950143677898	0.0
88	0.0	4.5554184880748255E-5	0.0	2.013016652787825	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4335	0.0	47.17471	1
GTATCAA	7755	0.0	39.76776	1
TCAACGC	8350	0.0	35.465717	4
ATCAACG	8440	0.0	35.031834	3
CAACGCA	8980	0.0	33.029934	5
AACGCAG	9270	0.0	31.993353	6
TATCAAC	9665	0.0	31.126678	2
ACGCAGA	10705	0.0	27.528723	7
CGCAGAG	11120	0.0	26.332277	8
GTACATG	11655	0.0	24.484175	1
TACATGG	11785	0.0	22.894806	2
GCAGAGT	12750	0.0	22.00743	9
ACATGGG	12415	0.0	21.240797	3
CATGGGG	9325	0.0	18.953686	4
CAGAGTA	12070	0.0	18.632874	10-11
AGAGTAC	11110	0.0	18.423803	10-11
GTGGTAT	2715	0.0	17.835138	1
GTCTCGC	1055	0.0	17.824469	1
GAGTACT	6105	0.0	17.476135	12-13
CCGGTCG	370	3.1650416E-9	16.513792	9
>>END_MODULE