FastQCFastQC Report
Wed 25 May 2016
SRR2049342_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049342_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4390376
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123400.28106932071421675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114820.2615265754003757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66510.15149044182092833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75700.058.258531
GTATCAA138600.040.3000681
ATCAACG168400.032.5119823
TCAACGC168350.032.493724
CAACGCA172900.031.6929875
TATCAAC175650.031.2503072
AACGCAG179050.030.68316
GTGGTAT30000.030.5608841
ACGCAGA206350.026.4869777
CGCAGAG207600.026.2821778
TGGTATC32350.026.1491642
GCAGAGT229550.023.523149
GTACATG117500.022.8081231
GAGTACT166150.021.7606112-13
TACATGG117100.021.4310762
CAGAGTA223600.021.09890210-11
AGAGTAC212450.020.4350510-11
ACATGGG121600.020.0582663
GTACTTT185100.019.37214514-15
AGTACTT176950.018.53270112-13
Produced by FastQC (version 0.11.5)