##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049342_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4390376 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.858167500915638 31.0 31.0 34.0 30.0 34.0 2 32.088282643673345 33.0 31.0 34.0 30.0 34.0 3 32.05630246703244 34.0 31.0 34.0 30.0 34.0 4 35.75114386558236 37.0 35.0 37.0 33.0 37.0 5 35.81927812105387 37.0 35.0 37.0 35.0 37.0 6 35.84889039116467 37.0 35.0 37.0 35.0 37.0 7 35.79713787611813 37.0 35.0 37.0 35.0 37.0 8 35.7972449284526 37.0 35.0 37.0 35.0 37.0 9 37.54173036660186 39.0 37.0 39.0 35.0 39.0 10-11 37.501008569653266 39.0 37.0 39.0 35.0 39.0 12-13 37.44550284531439 39.0 37.0 39.0 34.5 39.0 14-15 38.741634657259425 40.0 38.0 41.0 34.5 41.0 16-17 38.772124528741955 40.0 38.0 41.0 35.0 41.0 18-19 38.84860943572942 40.0 38.0 41.0 35.0 41.0 20-21 38.878635451724406 40.0 38.0 41.0 35.0 41.0 22-23 38.84850021957117 40.0 38.0 41.0 35.0 41.0 24-25 38.82576093710425 40.0 38.0 41.0 35.0 41.0 26-27 38.71088501303761 40.0 38.0 41.0 34.5 41.0 28-29 38.63134307858826 40.0 38.0 41.0 34.0 41.0 30-31 38.52835121638785 40.0 38.0 41.0 34.0 41.0 32-33 38.38359709965616 40.0 38.0 41.0 34.0 41.0 34-35 38.280827655763424 40.0 38.0 41.0 34.0 41.0 36-37 38.166555780188304 40.0 38.0 41.0 34.0 41.0 38-39 38.07219768876287 40.0 38.0 41.0 33.5 41.0 40-41 37.95492356463319 40.0 38.0 41.0 33.0 41.0 42-43 37.81453410824039 40.0 37.0 41.0 33.0 41.0 44-45 37.62883497905419 40.0 37.0 41.0 33.0 41.0 46-47 37.39612860037501 40.0 37.0 41.0 32.5 41.0 48-49 37.18334728050627 40.0 37.0 41.0 32.0 41.0 50-51 37.03487423400638 40.0 37.0 41.0 31.0 41.0 52-53 36.896966341834954 40.0 36.0 41.0 31.0 41.0 54-55 36.75711351374005 40.0 36.0 41.0 31.0 41.0 56-57 36.616768814333895 39.0 36.0 41.0 31.0 41.0 58-59 36.43028934196069 39.0 35.0 41.0 30.5 41.0 60-61 36.256206188262695 39.0 35.0 41.0 30.0 41.0 62-63 36.05953328370964 39.0 35.0 41.0 30.0 41.0 64-65 35.83937480525586 38.5 35.0 40.5 30.0 41.0 66-67 35.5776300253099 38.0 35.0 40.0 29.5 41.0 68-69 35.29836351146234 37.0 35.0 40.0 29.0 41.0 70-71 34.97863280958168 37.0 34.5 39.5 29.0 41.0 72-73 34.68606561260357 36.0 34.0 39.0 29.0 41.0 74-75 34.38330464178922 36.0 34.0 39.0 29.0 41.0 76-77 34.05693623962959 35.5 34.0 38.5 29.0 40.0 78-79 33.72272705116828 35.0 34.0 37.0 28.5 39.5 80-81 33.40707629597101 35.0 34.0 37.0 28.0 39.0 82-83 33.107283977499876 35.0 34.0 36.5 28.0 39.0 84-85 32.83280862504715 35.0 34.0 36.0 27.0 37.5 86-87 32.593967692061 35.0 33.0 36.0 27.0 37.0 88-89 32.36577265819602 35.0 33.0 35.5 27.0 37.0 90-91 32.170161963348924 35.0 33.0 35.0 26.5 36.0 92-93 31.98596418621093 35.0 33.0 35.0 26.0 36.0 94-95 31.829081153869282 35.0 33.0 35.0 26.0 36.0 96-97 31.633915865064857 35.0 33.0 35.0 25.5 36.0 98-99 31.48531503907638 35.0 33.0 35.0 25.0 35.5 100 31.37344113579338 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15686993702162866 1101 2 0.1480272522696282 1101 3 0.29091856465072397 1101 4 0.16077141583888732 1101 5 0.121774205260607 1101 6 0.10796526590037558 1101 7 0.016230879309638624 1101 8 0.12122882445702743 1101 9 0.1973926954384737 1101 10-11 0.22830846522732884 1101 12-13 0.16904991647048462 1101 14-15 0.2840115045418514 1101 16-17 0.20411546463574126 1101 18-19 0.2822840102531572 1101 20-21 0.29094475534797226 1101 22-23 0.2297494880480997 1101 24-25 0.23452676508132697 1101 26-27 0.3126094345894401 1101 28-29 0.3170980663208667 1101 30-31 0.2657463814372534 1101 32-33 0.3091436506579157 1101 34-35 0.3007863634287702 1101 36-37 0.3561749572799755 1101 38-39 0.31727148711092923 1101 40-41 0.2842796906326015 1101 42-43 0.3735333179242062 1101 44-45 0.3582303577978294 1101 46-47 0.3759944638983086 1101 48-49 0.3718813730560342 1101 50-51 0.34106889650131933 1101 52-53 0.39989057813362905 1101 54-55 0.35205239198007376 1101 56-57 0.36757050491230103 1101 58-59 0.41339580118354036 1101 60-61 0.35704700089939934 1101 62-63 0.36809058661825844 1101 64-65 0.31095573056086323 1101 66-67 0.2951303518204398 1101 68-69 0.26866969318470524 1101 70-71 0.2675145883689112 1101 72-73 0.211283681272171 1101 74-75 0.31833351823902234 1101 76-77 0.2862283124143872 1101 78-79 0.2719113968020963 1101 80-81 0.2953800907783801 1101 82-83 0.30488312271766205 1101 84-85 0.30964740792486367 1101 86-87 0.22517600346727562 1101 88-89 0.2712091603738003 1101 90-91 0.3255741571130457 1101 92-93 0.3687680737057555 1101 94-95 0.3620811390751051 1101 96-97 0.41141781424927615 1101 98-99 0.42201729038139746 1101 100 0.44526874880769185 1102 1 0.15464418423243487 1102 2 0.17513271694996035 1102 3 0.3101277120015311 1102 4 0.12533693681235292 1102 5 0.1209771884018025 1102 6 0.12499362615219667 1102 7 0.13220203753393633 1102 8 0.1346272316158732 1102 9 0.14900066852005267 1102 10-11 0.2020946897339826 1102 12-13 0.18244201865755372 1102 14-15 0.2678901071489648 1102 16-17 0.23105027963418934 1102 18-19 0.17739687022550044 1102 20-21 0.15421371996994537 1102 22-23 0.23534842517648258 1102 24-25 0.2251595320489912 1102 26-27 0.2631279427607822 1102 28-29 0.2564236366862147 1102 30-31 0.2751619770244389 1102 32-33 0.29313315234227133 1102 34-35 0.31386336365419254 1102 36-37 0.30582841354820545 1102 38-39 0.31121255585937035 1102 40-41 0.37425046488900193 1102 42-43 0.40895271878179784 1102 44-45 0.3491584564861725 1102 46-47 0.3518278003271007 1102 48-49 0.30414180710006633 1102 50-51 0.35508775687131333 1102 52-53 0.3743741862822745 1102 54-55 0.2979321515739386 1102 56-57 0.36910180660451175 1102 58-59 0.30816540676210025 1102 60-61 0.3706039854298311 1102 62-63 0.3748254450574606 1102 64-65 0.2961785873641034 1102 66-67 0.33059774866474356 1102 68-69 0.3513570031435691 1102 70-71 0.3349820630234177 1102 72-73 0.3917612745754937 1102 74-75 0.300398903826661 1102 76-77 0.26437430271626994 1102 78-79 0.2975884432444147 1102 80-81 0.3020320803830998 1102 82-83 0.3415135424762923 1102 84-85 0.3518131774608406 1102 86-87 0.2806820424572223 1102 88-89 0.24694452536128608 1102 90-91 0.3337113585775242 1102 92-93 0.35182512382744235 1102 94-95 0.29039263244238 1102 96-97 0.3627575572136621 1102 98-99 0.3589530320640648 1102 100 0.38133164290508503 1103 1 0.08752663847583264 1103 2 0.12409655243813944 1103 3 0.2745837022536577 1103 4 0.15598074552804775 1103 5 0.15765296435383647 1103 6 0.07666524586156953 1103 7 0.12625640620730394 1103 8 0.14462578401626303 1103 9 0.18938323464944773 1103 10-11 0.16049175514324787 1103 12-13 0.13834149760758407 1103 14-15 0.14974378040196967 1103 16-17 0.13781440353535857 1103 18-19 0.15822775757352758 1103 20-21 0.18003905929941055 1103 22-23 0.17741606357659379 1103 24-25 0.21249279686705336 1103 26-27 0.16046747271745687 1103 28-29 0.21429558939121307 1103 30-31 0.22636323421269822 1103 32-33 0.22655684614127125 1103 34-35 0.23981728360985244 1103 36-37 0.2567530809223513 1103 38-39 0.2545344982918536 1103 40-41 0.30789920698983053 1103 42-43 0.337927381685283 1103 44-45 0.3545002493549987 1103 46-47 0.34938460620666945 1103 48-49 0.22680242600663547 1103 50-51 0.30004627651033644 1103 52-53 0.2791072095983651 1103 54-55 0.246948458969662 1103 56-57 0.27010093899899346 1103 58-59 0.32615561318293373 1103 60-61 0.29242314531249036 1103 62-63 0.26239479886789496 1103 64-65 0.2492214427061512 1103 66-67 0.16667831259306354 1103 68-69 0.21975314102032684 1103 70-71 0.25733121422142347 1103 72-73 0.2838295904942143 1103 74-75 0.18864376038690267 1103 76-77 0.24170567525349185 1103 78-79 0.2504157450876363 1103 80-81 0.2802287205206113 1103 82-83 0.21742839537316883 1103 84-85 0.21758346554079822 1103 86-87 0.19689078765745904 1103 88-89 0.23402039274970576 1103 90-91 0.25935749662126284 1103 92-93 0.31399409515475796 1103 94-95 0.22419021392997607 1103 96-97 0.2903040275945976 1103 98-99 0.29698108505594334 1103 100 0.3221021075215802 1104 1 0.13807623437208605 1104 2 0.17618047541701998 1104 3 0.22691964667621534 1104 4 0.16952203644019903 1104 5 0.13436582685866938 1104 6 0.1620308677225779 1104 7 0.13400599044938843 1104 8 0.09284503134181676 1104 9 0.20587044095972118 1104 10-11 0.17351285150474638 1104 12-13 0.18902794907906184 1104 14-15 0.2575565260876118 1104 16-17 0.22598254130985396 1104 18-19 0.22884308391805774 1104 20-21 0.24202742039002345 1104 22-23 0.22781058269405463 1104 24-25 0.18774718845459404 1104 26-27 0.21289609554593625 1104 28-29 0.26407988317621545 1104 30-31 0.30368246044974967 1104 32-33 0.30775871487830386 1104 34-35 0.24530313766633327 1104 36-37 0.2403104217550478 1104 38-39 0.22955394503321713 1104 40-41 0.23045600488615747 1104 42-43 0.2743220737654397 1104 44-45 0.2548918780531153 1104 46-47 0.2328848439147535 1104 48-49 0.2513916049240592 1104 50-51 0.1930220140991068 1104 52-53 0.1854558653915035 1104 54-55 0.2456582798284117 1104 56-57 0.2657656683910048 1104 58-59 0.31503096720149415 1104 60-61 0.21879259131390683 1104 62-63 0.2742275282191926 1104 64-65 0.20781662490893638 1104 66-67 0.28153679072484294 1104 68-69 0.18732411584546327 1104 70-71 0.2957394649118896 1104 72-73 0.30845731604403426 1104 74-75 0.2706437895223317 1104 76-77 0.23521275284992527 1104 78-79 0.2759846258617671 1104 80-81 0.24125212089317927 1104 82-83 0.28086677579489816 1104 84-85 0.26754022486737483 1104 86-87 0.23746322249122187 1104 88-89 0.225809971608669 1104 90-91 0.24171107028950445 1104 92-93 0.31074436199827105 1104 94-95 0.3200144135381713 1104 96-97 0.3392058301063514 1104 98-99 0.2817377071222822 1104 100 0.27470036328068304 1105 1 0.16380902819691912 1105 2 0.17384090001305452 1105 3 0.29624713385131685 1105 4 0.19248991593335774 1105 5 0.13946555674854721 1105 6 0.1171267719420257 1105 7 0.1395797475958318 1105 8 0.1664213258864109 1105 9 0.20164670062843726 1105 10-11 0.19915037056637175 1105 12-13 0.20705914564200611 1105 14-15 0.3148854986015053 1105 16-17 0.294546506964231 1105 18-19 0.2544841728640037 1105 20-21 0.22640690776798067 1105 22-23 0.22292145373693728 1105 24-25 0.27462472740970867 1105 26-27 0.3203050178990665 1105 28-29 0.3051613001155715 1105 30-31 0.28281823260348915 1105 32-33 0.34941817504952155 1105 34-35 0.2939852164466714 1105 36-37 0.25689117765414693 1105 38-39 0.29905834722327995 1105 40-41 0.30591122630350753 1105 42-43 0.3563506421817735 1105 44-45 0.35432279845603887 1105 46-47 0.3555781425857276 1105 48-49 0.35661287769791983 1105 50-51 0.384953419383514 1105 52-53 0.3596202233105501 1105 54-55 0.3828828204365706 1105 56-57 0.3262873630562382 1105 58-59 0.36880699288153096 1105 60-61 0.32691545716810566 1105 62-63 0.35645955779963856 1105 64-65 0.3624022247295713 1105 66-67 0.33370124161658055 1105 68-69 0.30073326862850536 1105 70-71 0.3785341238311304 1105 72-73 0.3675292373135264 1105 74-75 0.3290571839062508 1105 76-77 0.31874548813310355 1105 78-79 0.29947869073446043 1105 80-81 0.2983460593408154 1105 82-83 0.3102816105882127 1105 84-85 0.3127355831725538 1105 86-87 0.2756378639013448 1105 88-89 0.31312519395129357 1105 90-91 0.33991649722542405 1105 92-93 0.3844675444934289 1105 94-95 0.37554651091305047 1105 96-97 0.4262865188337912 1105 98-99 0.4772290210710324 1105 100 0.44833769061394335 1106 1 0.12291199291466981 1106 2 0.12631025569394438 1106 3 0.2361449531691946 1106 4 0.1760550081220984 1106 5 0.07770461728004818 1106 6 0.1205449166028103 1106 7 0.11367091936769214 1106 8 0.09198405267954257 1106 9 0.10224485595694688 1106 10-11 0.09613984448030521 1106 12-13 0.1248118858502778 1106 14-15 0.16077751171263088 1106 16-17 0.19538518847496533 1106 18-19 0.20489091092469636 1106 20-21 0.2136128660497647 1106 22-23 0.19923925642336116 1106 24-25 0.1760285692814847 1106 26-27 0.23601780697101304 1106 28-29 0.22982047906378966 1106 30-31 0.27758142132617536 1106 32-33 0.2310814865795976 1106 34-35 0.24800659964886052 1106 36-37 0.26613598075770284 1106 38-39 0.26409918524791465 1106 40-41 0.2799257015220036 1106 42-43 0.25153412239888695 1106 44-45 0.2677937231805174 1106 46-47 0.3150450134884366 1106 48-49 0.3561927590651308 1106 50-51 0.2552652628806271 1106 52-53 0.25345235757824014 1106 54-55 0.27966737766819705 1106 56-57 0.2630942107976395 1106 58-59 0.30819469747005 1106 60-61 0.24104445539054353 1106 62-63 0.26104788940057233 1106 64-65 0.22414404137803245 1106 66-67 0.258028547804372 1106 68-69 0.31044792050080616 1106 70-71 0.3148759413222848 1106 72-73 0.3578626733915016 1106 74-75 0.3090097809202561 1106 76-77 0.29780485498194054 1106 78-79 0.32877897810615764 1106 80-81 0.2933494825416716 1106 82-83 0.3535173451535343 1106 84-85 0.3234155917335215 1106 86-87 0.27443413099722136 1106 88-89 0.33446905931281634 1106 90-91 0.2913863992571635 1106 92-93 0.3253314227504269 1106 94-95 0.3361789987260302 1106 96-97 0.33776293342819486 1106 98-99 0.37170121881352713 1106 100 0.4263481761855594 1107 1 0.0973893044092442 1107 2 0.15262316205140536 1107 3 0.2249227572493453 1107 4 0.17283634507661105 1107 5 0.11718835529015337 1107 6 0.15419496804911859 1107 7 0.13194365231179006 1107 8 0.09584194891866105 1107 9 0.1487784045899403 1107 10-11 0.1401209896648581 1107 12-13 0.12599573846088674 1107 14-15 0.17811481062764045 1107 16-17 0.15339077236491505 1107 18-19 0.13134104304428007 1107 20-21 0.16589895794209752 1107 22-23 0.10152008190586059 1107 24-25 0.12665345604833078 1107 26-27 0.21333505934506292 1107 28-29 0.1897067481125987 1107 30-31 0.15607819994980332 1107 32-33 0.1726592046880171 1107 34-35 0.16011671440318764 1107 36-37 0.15921894134744008 1107 38-39 0.09772827787861615 1107 40-41 0.1504813750584333 1107 42-43 0.21973591370463197 1107 44-45 0.21046962126897029 1107 46-47 0.2176252658830009 1107 48-49 0.20974493647455716 1107 50-51 0.21150353363519514 1107 52-53 0.20660653113860405 1107 54-55 0.16441056701453505 1107 56-57 0.17660921782236727 1107 58-59 0.2588479993487809 1107 60-61 0.20135649177556303 1107 62-63 0.18650531929002767 1107 64-65 0.17089651456100086 1107 66-67 0.19908584207041713 1107 68-69 0.18884104806030422 1107 70-71 0.12151112632389527 1107 72-73 0.22141457733218317 1107 74-75 0.2082090622956727 1107 76-77 0.220858900524739 1107 78-79 0.17675945414687533 1107 80-81 0.17951991168250458 1107 82-83 0.20378895516659412 1107 84-85 0.23844686544839533 1107 86-87 0.19366589533210998 1107 88-89 0.22875445984197995 1107 90-91 0.2318930599303073 1107 92-93 0.25510029038471416 1107 94-95 0.28285766739471185 1107 96-97 0.2680240234404323 1107 98-99 0.3015236265007317 1107 100 0.3467136500727115 1108 1 0.027620689565122092 1108 2 -0.005841774165254776 1108 3 0.09243081543885268 1108 4 0.003077792310392624 1108 5 0.05122252371910463 1108 6 0.0644139035153799 1108 7 0.05123380682437784 1108 8 -0.01726599671200546 1108 9 0.08376002850698683 1108 10-11 0.07124645013283981 1108 12-13 0.03166876782850636 1108 14-15 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-0.2460778459426365 2215 6 -0.27624413037993634 2215 7 -0.20587807333244257 2215 8 -0.18712163070489396 2215 9 -0.4234379594455149 2215 10-11 -0.3220763871260317 2215 12-13 -0.2652289924091633 2215 14-15 -0.37277010088040896 2215 16-17 -0.43352906716182815 2215 18-19 -0.4353515334998477 2215 20-21 -0.38543103710470916 2215 22-23 -0.36799715524207244 2215 24-25 -0.34860011117943657 2215 26-27 -0.39599860404123177 2215 28-29 -0.3669744285654133 2215 30-31 -0.437446558501982 2215 32-33 -0.3782867818167688 2215 34-35 -0.43143590560617895 2215 36-37 -0.39630005053674466 2215 38-39 -0.34035927398913657 2215 40-41 -0.34194846508906096 2215 42-43 -0.34235669966847837 2215 44-45 -0.4468881879792903 2215 46-47 -0.43830929995736767 2215 48-49 -0.431462675797853 2215 50-51 -0.4338895557629172 2215 52-53 -0.3629773085532477 2215 54-55 -0.37953689280954706 2215 56-57 -0.4214589284350936 2215 58-59 -0.36170638483607576 2215 60-61 -0.3462892850823991 2215 62-63 -0.4208920627164403 2215 64-65 -0.2933795319452699 2215 66-67 -0.26055662401876134 2215 68-69 -0.31484676983365745 2215 70-71 -0.37576489682429326 2215 72-73 -0.3808727032937824 2215 74-75 -0.3521137360606872 2215 76-77 -0.21246518156645777 2215 78-79 -0.3254977209453358 2215 80-81 -0.378724436940459 2215 82-83 -0.373515152080941 2215 84-85 -0.38968035836943216 2215 86-87 -0.31989313391220975 2215 88-89 -0.4000449889071831 2215 90-91 -0.4077123835917611 2215 92-93 -0.3853146068269062 2215 94-95 -0.43957233113980365 2215 96-97 -0.40821445910307474 2215 98-99 -0.3366512142163174 2215 100 -0.24105052028645346 2216 1 -0.3186140043543446 2216 2 -0.41322175929560956 2216 3 -0.43223455595310156 2216 4 -0.21306562992588596 2216 5 -0.4404330591750494 2216 6 -0.3128897206523078 2216 7 -0.5443531128179302 2216 8 -0.27186001845783636 2216 9 -0.5360645754141586 2216 10-11 -0.3735646396642025 2216 12-13 -0.3755753572243776 2216 14-15 -0.6173960557648357 2216 16-17 -0.45755980195176704 2216 18-19 -0.44677754717129403 2216 20-21 -0.4484779748544341 2216 22-23 -0.4654060796785586 2216 24-25 -0.4442635359285845 2216 26-27 -0.5668044455040189 2216 28-29 -0.4933330691395241 2216 30-31 -0.6080594424460202 2216 32-33 -0.5258953724897353 2216 34-35 -0.5255258451190628 2216 36-37 -0.6402407220435506 2216 38-39 -0.5893711927718073 2216 40-41 -0.5234127481224959 2216 42-43 -0.6175340818643988 2216 44-45 -0.7363796656342956 2216 46-47 -0.6174590753752369 2216 48-49 -0.7171567726040635 2216 50-51 -0.5534413718024069 2216 52-53 -0.5380226858421366 2216 54-55 -0.43394424676642984 2216 56-57 -0.5633314883846694 2216 58-59 -0.6465675371140236 2216 60-61 -0.45921534645857065 2216 62-63 -0.3578066297972242 2216 64-65 -0.4568379980850068 2216 66-67 -0.38729118013439745 2216 68-69 -0.34494615827295405 2216 70-71 -0.4371509741657462 2216 72-73 -0.461401226172093 2216 74-75 -0.421498061505865 2216 76-77 -0.41947761808796 2216 78-79 -0.5593926174954476 2216 80-81 -0.712011086692371 2216 82-83 -0.6227967104517163 2216 84-85 -0.6605718294274681 2216 86-87 -0.5198284512380269 2216 88-89 -0.875731436002642 2216 90-91 -0.6515114822730297 2216 92-93 -0.6953211775939749 2216 94-95 -0.7417661357834824 2216 96-97 -0.7823148898019809 2216 98-99 -0.8463065464253887 2216 100 -0.76021371622247 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 229.0 3 18.0 4 38.0 5 78.0 6 192.0 7 624.0 8 1536.0 9 2647.0 10 3760.0 11 4722.0 12 6901.0 13 9983.0 14 12230.0 15 15704.0 16 19242.0 17 25942.0 18 26056.0 19 14705.0 20 10905.0 21 10646.0 22 11097.0 23 12170.0 24 14619.0 25 18004.0 26 23304.0 27 30315.0 28 39702.0 29 51867.0 30 67466.0 31 87618.0 32 112694.0 33 148611.0 34 202305.0 35 293176.0 36 470886.0 37 859251.0 38 1245578.0 39 533504.0 40 2051.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.41914003416968 20.515183815839183 14.932305478222865 23.13337067176827 2 15.331875903111717 20.608348806571463 40.087956020167745 23.971819270149073 3 17.734061957335772 25.397186937975242 31.108041771365368 25.760709333323618 4 12.359909365311186 17.393332501209464 38.6084596020565 31.638298531422848 5 12.489408652015225 37.7521196362225 35.863329245604476 13.895142466157795 6 30.33203314067705 38.539418614583035 17.278136833414003 13.850411411325911 7 26.100019679407872 33.19137130851663 22.54292115299464 18.16568785908086 8 24.737832799736147 36.35398084152255 20.302514982935215 18.60567137580609 9 24.466583272138877 16.682739701565424 21.822253948181203 37.02842307811449 10-11 23.563995430004173 27.204833936774435 28.74056800602044 20.490602627200953 12-13 24.27907169639257 24.684988902134496 29.22201689819644 21.813922503276494 14-15 21.47816859254751 26.631069075695635 27.04025991419406 24.850502417562794 16-17 20.405427690020172 30.04625344161867 27.705280823328117 21.843038045033044 18-19 20.25343168333362 28.286186064537315 30.79358036541074 20.666801886718318 20-21 21.35214205530758 27.437067156799493 30.70625296315838 20.50453782473454 22-23 21.000524784209826 27.298220015779968 30.787306144166237 20.913949055843965 24-25 20.947674006100396 27.413198954393486 30.657082357855675 20.982044681650443 26-27 20.717790785021958 27.421240195026662 30.727655548251697 21.133313471699676 28-29 20.698609738945247 27.39897901369192 30.87489928223467 21.027511965128166 30-31 20.97449056755048 27.702718400428573 30.49007647636558 20.83271455565537 32-33 20.70088861541153 27.540221842375683 30.84905495995269 20.909834582260103 34-35 20.87797551597552 27.33419233399987 30.784673648457662 21.00315850156695 36-37 21.115326774664336 27.68799230589204 30.243128572803336 20.953552346640294 38-39 21.034650210016803 27.77654148415518 30.301788257599654 20.887020048228358 40-41 20.95407199072698 27.572692603882448 30.36148881267665 21.111746592713917 42-43 20.93546311062479 27.61671935229758 30.1743858888683 21.27343164820933 44-45 21.1244371339926 27.71917281146638 29.939876304148257 21.216513750392764 46-47 21.19674838450459 27.926930557015506 29.543923560910507 21.332397497569396 48-49 21.257758936920723 27.881983185586627 29.31936600361635 21.540891873876298 50-51 21.363837010028345 27.973688238493644 29.107092910005477 21.55538184147254 52-53 21.325084793865095 28.02632271254726 29.081397327276413 21.56719516631123 54-55 21.412624130081536 28.36004795968617 28.868104940968315 21.359222969263982 56-57 21.306034082684704 28.106923077361103 28.972480000182216 21.614562839771978 58-59 21.211874805113283 27.744933173073527 29.285919117086006 21.757272904727184 60-61 21.36882375235971 28.058160500440966 28.991669946573957 21.581345800625368 62-63 21.208744302539237 28.114382212009886 29.03439215203038 21.642481333420495 64-65 21.22979079261559 27.887678369626705 29.1568577631452 21.725673074612505 66-67 21.19094925578199 27.98060183863884 28.97284316630728 21.85560573927189 68-69 21.564518579443952 28.05205738366024 28.84943861582408 21.533985421071726 70-71 21.481443013314756 27.95410940050513 28.891846888446025 21.67260069773409 72-73 21.3239282664156 28.11295327900862 28.92665093284022 21.63646752173556 74-75 21.474927348880147 28.1495486160897 28.74191846333244 21.633605571697718 76-77 21.47833484612257 28.160178755883646 28.620985366753636 21.740501031240143 78-79 21.53270015595962 28.19718926463912 28.68896203600761 21.581148543393653 80-81 21.423247146782877 28.106763978973014 28.716089592344513 21.753899281899596 82-83 21.328714693249964 28.14566938146158 28.83490073799767 21.69071518729078 84-85 21.42818990188842 28.062153088321836 28.681604366396357 21.828052643393388 86-87 21.50125486282835 28.180881099682814 28.568210452972625 21.74965358451621 88-89 21.291094371648715 28.088570894275573 28.687711133498894 21.932623600576818 90-91 21.25230211387325 27.998852459371125 28.72812614564723 22.020719281108388 92-93 21.36216725696845 28.195633137244343 28.573127133340474 21.869072472446728 94-95 21.197425322355752 28.05060202570737 28.66701767417324 22.08495497776364 96-97 21.227058243634527 28.056000481066217 28.655727421321075 22.06121385397818 98-99 21.182918429283788 28.140070319425853 28.57644618122149 22.10056507006887 100 21.23301290862919 28.046230809196327 28.57191015049248 22.148846131682003 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 725.0 1 523.5 2 654.5 3 959.5 4 598.0 5 365.5 6 983.5 7 2682.0 8 4592.5 9 5224.0 10 3861.5 11 2312.0 12 2102.5 13 2340.5 14 2757.5 15 3254.0 16 3702.5 17 4241.5 18 4926.0 19 5749.0 20 7047.0 21 8932.0 22 11496.5 23 15241.5 24 20360.5 25 27023.0 26 35837.0 27 46394.0 28 58470.0 29 72724.0 30 86838.5 31 98475.5 32 107989.5 33 117208.0 34 126322.0 35 133343.0 36 139212.0 37 146198.0 38 152915.5 39 156175.0 40 157683.5 41 158836.0 42 157608.0 43 154740.5 44 151453.5 45 147863.5 46 144674.0 47 139290.5 48 132680.5 49 128475.0 50 124331.5 51 119519.0 52 112455.5 53 105450.0 54 100016.0 55 94253.0 56 87719.0 57 80496.0 58 74343.0 59 68552.5 60 60668.0 61 51593.0 62 44425.0 63 37865.5 64 30895.0 65 24814.0 66 19754.5 67 15216.0 68 11684.5 69 8926.0 70 6618.5 71 4858.5 72 3569.5 73 2591.0 74 1913.0 75 1405.0 76 966.5 77 649.0 78 448.0 79 307.0 80 183.5 81 79.5 82 41.5 83 25.0 84 17.5 85 12.0 86 5.5 87 2.5 88 2.0 89 1.5 90 0.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03849328622423227 2 0.0 3 0.0 4 9.110836976149651E-5 5 0.0 6 2.2777092440374127E-5 7 0.0 8 0.0010933004371379582 9 0.0 10-11 0.0 12-13 0.04372062893929814 14-15 9.110836976149651E-5 16-17 0.0 18-19 3.416563866056119E-5 20-21 3.416563866056119E-5 22-23 0.0 24-25 1.2527400842205768E-4 26-27 4.5554184880748255E-5 28-29 3.0749074794505073E-4 30-31 0.0 32-33 1.4805110086243183E-4 34-35 6.833127732112239E-5 36-37 7.971982354130945E-5 38-39 2.9610220172486367E-4 40-41 1.1388546220187065E-4 42-43 6.035929496699143E-4 44-45 2.3915947062392833E-4 46-47 1.0249691598168357E-4 48-49 8.085867816332816E-4 50-51 4.555418488074826E-4 52-53 5.23873126128605E-4 54-55 5.010960336882307E-4 56-57 5.35261672348792E-4 58-59 1.1388546220187065E-4 60-61 1.8221673952299302E-4 62-63 9.794149749360874E-4 64-65 4.441533025872955E-4 66-67 0.00143495682374357 68-69 0.0015260651935050666 70-71 4.897074874680437E-4 72-73 0.001081911890917771 74-75 0.0010135806135966486 76-77 7.06089865651598E-4 78-79 0.003120461664331255 80-81 0.0025624228995420892 82-83 0.0011502431682388935 84-85 0.0027218625466247084 86-87 0.001264128630440764 88-89 0.0019360528574318006 90-91 0.00267630836174396 92-93 0.0024485374373402185 94-95 0.003564614966918551 96-97 0.0029154678323678883 98-99 0.002824359462606392 100 0.0012982942691013254 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4390376.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.23781412111902 #Duplication Level Percentage of deduplicated Percentage of total 1 77.45858728907257 49.75770332359915 2 13.898327812346842 17.8559639720783 3 4.130966615953741 7.960927968485533 4 1.674495036372548 4.302636035729446 5 0.8409911912557151 2.7011717910691537 6 0.5049524240646415 1.9462223974243742 7 0.31526607333047607 1.4176402392108738 8 0.21570564233373868 1.108516716568903 9 0.15909359242745072 0.9197842156394082 >10 0.7590367434403489 8.432857996274612 >50 0.03138499897201135 1.3554749479629182 >100 0.01041268161996102 1.1171524964351027 >500 4.60849400255087E-4 0.21547124037882961 >1k 2.12699606489478E-4 0.2145374894921437 >5k 3.544993441484167E-5 0.151458321049815 >10k+ 7.089986882968334E-5 0.5424808486015175 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12340 0.28106932071421675 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11482 0.2615265754003757 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6651 0.15149044182092833 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.833127732112239E-5 0.0 10-11 0.0 0.0 0.0 6.833127732112239E-5 0.0 12-13 0.0 0.0 0.0 6.833127732112239E-5 0.0 14-15 0.0 0.0 0.0 6.833127732112239E-5 0.0 16-17 0.0 0.0 0.0 6.833127732112239E-5 0.0 18-19 0.0 0.0 0.0 7.971982354130945E-5 0.0 20-21 0.0 0.0 0.0 1.7082819330280596E-4 0.0 22-23 0.0 0.0 0.0 3.416563866056119E-4 0.0 24-25 0.0 0.0 0.0 6.263700421102886E-4 0.0 26-27 0.0 0.0 0.0 0.0010135806135966488 0.0 28-29 0.0 2.2777092440374127E-5 0.0 0.0021866008742759163 0.0 30-31 0.0 2.2777092440374127E-5 0.0 0.005625941832772409 0.0 32-33 0.0 2.2777092440374127E-5 0.0 0.013905414934848405 0.0 34-35 0.0 2.2777092440374127E-5 0.0 0.027628613130173816 0.0 36-37 0.0 2.2777092440374127E-5 0.0 0.04823049324249222 0.0 38-39 0.0 2.2777092440374127E-5 0.0 0.07573383236424397 0.0 40-41 0.0 2.2777092440374127E-5 0.0 0.10577681729309744 0.0 42-43 0.0 2.2777092440374127E-5 0.0 0.13515926654118007 0.0 44-45 0.0 2.2777092440374127E-5 0.0 0.1706231994708426 0.0 46-47 0.0 2.2777092440374127E-5 0.0 0.21495880990603083 0.0 48-49 0.0 2.2777092440374127E-5 0.0 0.26141268993817385 0.0 50-51 0.0 4.5554184880748255E-5 0.0 0.30809434089472065 0.0 52-53 0.0 4.5554184880748255E-5 0.0 0.3520199636659821 0.0 54-55 0.0 5.6942731100935323E-5 0.0 0.401081820782548 0.0 56-57 0.0 6.833127732112239E-5 0.0 0.4615777783041817 0.0 58-59 0.0 6.833127732112239E-5 0.0 0.5259344529944587 0.0 60-61 0.0 6.833127732112239E-5 0.0 0.5911338801050297 0.0 62-63 0.0 6.833127732112239E-5 0.0 0.6546022481901322 0.0 64-65 0.0 6.833127732112239E-5 0.0 0.7223982638389058 0.0 66-67 0.0 6.833127732112239E-5 0.0 0.8021066988339951 0.0 68-69 0.0 6.833127732112239E-5 0.0 0.8878738404182238 0.0 70-71 0.0 6.833127732112239E-5 0.0 0.9781736233980871 0.0 72-73 0.0 6.833127732112239E-5 0.0 1.0732452072442087 0.0 74-75 0.0 6.833127732112239E-5 0.0 1.1706514430654686 0.0 76-77 0.0 6.833127732112239E-5 0.0 1.276599088551869 0.0 78-79 1.1388546220187064E-5 6.833127732112239E-5 0.0 1.392819202728878 0.0 80-81 2.2777092440374127E-5 6.833127732112239E-5 0.0 1.5177857204029905 0.0 82-83 2.2777092440374127E-5 6.833127732112239E-5 0.0 1.6464079614137832 0.0 84-85 2.2777092440374127E-5 6.833127732112239E-5 0.0 1.773936901987438 0.0 86-87 2.2777092440374127E-5 6.833127732112239E-5 0.0 1.91226218437783 0.0 88 2.2777092440374127E-5 6.833127732112239E-5 0.0 2.0249290721341406 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7570 0.0 58.25853 1 GTATCAA 13860 0.0 40.300068 1 ATCAACG 16840 0.0 32.511982 3 TCAACGC 16835 0.0 32.49372 4 CAACGCA 17290 0.0 31.692987 5 TATCAAC 17565 0.0 31.250307 2 AACGCAG 17905 0.0 30.6831 6 GTGGTAT 3000 0.0 30.560884 1 ACGCAGA 20635 0.0 26.486977 7 CGCAGAG 20760 0.0 26.282177 8 TGGTATC 3235 0.0 26.149164 2 GCAGAGT 22955 0.0 23.52314 9 GTACATG 11750 0.0 22.808123 1 GAGTACT 16615 0.0 21.76061 12-13 TACATGG 11710 0.0 21.431076 2 CAGAGTA 22360 0.0 21.098902 10-11 AGAGTAC 21245 0.0 20.43505 10-11 ACATGGG 12160 0.0 20.058266 3 GTACTTT 18510 0.0 19.372145 14-15 AGTACTT 17695 0.0 18.532701 12-13 >>END_MODULE