FastQCFastQC Report
Wed 25 May 2016
SRR2049341_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049341_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4339329
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38850.040.5332831
GTATCAA62800.034.9554821
TCAACGC64950.032.4225274
ATCAACG67200.031.4069023
CAACGCA70450.029.824945
AACGCAG73900.028.5574826
TATCAAC78950.027.208972
GTACATG107050.025.0730691
ACGCAGA85400.024.2713367
TACATGG107950.023.9490382
CGCAGAG89150.023.4085568
ACATGGG111050.022.0953043
GCAGAGT103450.019.3095179
CATGGGG86350.019.2158854
GAGTACA81200.018.5826241
AGAGTAC88050.017.2152510-11
TACGTCG2556.0897237E-616.5903425
CAGAGTA98350.016.51150510-11
AGTACAT85450.016.2827472
AGTACCG5550.016.0921345
Produced by FastQC (version 0.11.5)