##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049341_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4339329
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.610874630616852	31.0	31.0	34.0	27.0	34.0
2	30.707620694351593	31.0	31.0	34.0	27.0	34.0
3	30.897538536488014	31.0	31.0	34.0	27.0	34.0
4	34.49511756310711	37.0	35.0	37.0	32.0	37.0
5	34.49879393795677	37.0	35.0	37.0	32.0	37.0
6	34.521113748231585	37.0	35.0	37.0	31.0	37.0
7	34.37988062209618	37.0	35.0	37.0	30.0	37.0
8	34.366555981351034	37.0	35.0	37.0	30.0	37.0
9	35.91495274960714	39.0	37.0	39.0	30.0	39.0
10-11	35.90554806975917	39.0	37.0	39.0	30.0	39.0
12-13	35.8578235252501	39.0	37.0	39.0	30.0	39.0
14-15	37.02885780266949	40.0	37.0	41.0	31.0	41.0
16-17	36.98027506095988	40.0	37.0	41.0	31.0	41.0
18-19	36.984577915156926	40.0	37.0	41.0	31.0	41.0
20-21	37.070283101373505	40.0	37.0	41.0	31.0	41.0
22-23	37.00309033954328	40.0	37.0	41.0	31.0	41.0
24-25	36.99652296472565	40.0	37.0	41.0	30.5	41.0
26-27	36.983056251323646	40.0	37.0	41.0	31.0	41.0
28-29	36.92113665499896	40.0	37.0	41.0	30.0	41.0
30-31	36.85622327783858	40.0	37.0	41.0	30.0	41.0
32-33	36.80929148262324	40.0	37.0	41.0	30.0	41.0
34-35	36.706908948365054	40.0	37.0	41.0	30.0	41.0
36-37	36.614393607859654	40.0	37.0	41.0	30.0	41.0
38-39	36.502470888932365	40.0	36.0	41.0	30.0	41.0
40-41	36.394017254741456	40.0	36.0	41.0	30.0	41.0
42-43	36.23696417118868	40.0	36.0	41.0	29.0	41.0
44-45	36.08816616578277	40.0	36.0	41.0	29.0	41.0
46-47	35.96108292318928	39.0	35.0	41.0	28.5	41.0
48-49	35.82306550160175	39.0	35.0	41.0	28.0	41.0
50-51	35.670896122418924	39.0	35.0	41.0	28.0	41.0
52-53	35.51773615229452	39.0	35.0	41.0	27.5	41.0
54-55	35.36764105694682	39.0	35.0	40.5	27.0	41.0
56-57	35.227536331077914	39.0	35.0	40.0	27.0	41.0
58-59	35.01596790655883	38.5	34.0	40.0	26.5	41.0
60-61	34.78870005016905	38.0	34.0	40.0	26.0	41.0
62-63	34.56665673425546	38.0	34.0	40.0	26.0	41.0
64-65	34.317679876312674	37.5	34.0	40.0	26.0	41.0
66-67	34.05035052654454	37.0	34.0	40.0	25.5	41.0
68-69	33.76091107634383	36.5	33.0	39.5	25.0	41.0
70-71	33.473408100653344	36.0	33.0	39.0	25.0	41.0
72-73	33.131566654660205	35.5	33.0	39.0	24.0	40.5
74-75	32.79349722042279	35.0	33.0	38.5	23.0	40.0
76-77	32.469496551194894	35.0	32.5	37.5	22.5	39.5
78-79	32.16396728618641	35.0	32.0	37.0	22.0	39.0
80-81	31.822498132775827	35.0	32.0	36.5	21.0	39.0
82-83	31.51860011075445	35.0	32.0	36.0	20.0	38.5
84-85	31.23871340476834	35.0	32.0	36.0	20.0	37.0
86-87	30.933510342267205	35.0	32.0	35.0	18.5	37.0
88-89	30.690628551096264	35.0	31.5	35.0	17.5	36.5
90-91	30.49400517914175	35.0	31.0	35.0	13.0	36.0
92-93	30.26424016247673	35.0	31.0	35.0	7.0	36.0
94-95	30.063327648122552	34.0	31.0	35.0	2.0	36.0
96-97	29.80691749807401	34.0	31.0	35.0	2.0	35.0
98-99	29.597386485329874	34.0	31.0	35.0	2.0	35.0
100	29.47202712677467	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.41644171777384287
1101	2	0.42394069864351636
1101	3	0.4068024552308067
1101	4	0.4797685134762659
1101	5	0.4624692128633541
1101	6	0.5408818337569556
1101	7	0.4635503738679745
1101	8	0.5525943833873654
1101	9	0.6458408215403111
1101	10-11	0.6569430981815714
1101	12-13	0.6573213867672862
1101	14-15	0.8272753627744933
1101	16-17	0.8023815192096322
1101	18-19	0.7949188444557294
1101	20-21	0.8018559690609806
1101	22-23	0.804871973576887
1101	24-25	0.7989254788504994
1101	26-27	0.7064458783011247
1101	28-29	0.7131917425204222
1101	30-31	0.726216880833185
1101	32-33	0.7302098708377542
1101	34-35	0.764549598581894
1101	36-37	0.7344265693134489
1101	38-39	0.8010181789657409
1101	40-41	0.8557901214220607
1101	42-43	0.7893196216880227
1101	44-45	0.6848892074181592
1101	46-47	0.6454280010119291
1101	48-49	0.6384028004525817
1101	50-51	0.6923643790351903
1101	52-53	0.7146317812342744
1101	54-55	0.6671462145076674
1101	56-57	0.6472203020668346
1101	58-59	0.6973708754096677
1101	60-61	0.7493841205348986
1101	62-63	0.7570638312161364
1101	64-65	0.6949548919782984
1101	66-67	0.7411850376939881
1101	68-69	0.6483522314577215
1101	70-71	0.6387688947685248
1101	72-73	0.7532277434697789
1101	74-75	0.7127955373804156
1101	76-77	0.7161683229913223
1101	78-79	0.6785972637225157
1101	80-81	0.7300548327232086
1101	82-83	0.7912388993395396
1101	84-85	0.775635400195327
1101	86-87	0.7627939383813711
1101	88-89	0.7312377851936631
1101	90-91	0.7850862015009135
1101	92-93	0.8001940217292471
1101	94-95	0.8095880094868662
1101	96-97	0.837160767088406
1101	98-99	0.8751824801773793
1101	100	0.8739569901708499
1102	1	0.15843541309976317
1102	2	0.23259540295359216
1102	3	0.2699041043478587
1102	4	0.2959806958811342
1102	5	0.20372940828461594
1102	6	0.3502791088967925
1102	7	0.35076406644213165
1102	8	0.425749717389877
1102	9	0.49873732419050754
1102	10-11	0.45770570381370135
1102	12-13	0.503089107816777
1102	14-15	0.5452246527459224
1102	16-17	0.5498480822424554
1102	18-19	0.5606238744869714
1102	20-21	0.49961041962653496
1102	22-23	0.5042271629273642
1102	24-25	0.5473334743246951
1102	26-27	0.5577931457103418
1102	28-29	0.5853329369325806
1102	30-31	0.5533019898678688
1102	32-33	0.5695807221440816
1102	34-35	0.644127960639608
1102	36-37	0.5344810964990998
1102	38-39	0.49052894992704665
1102	40-41	0.5479993020367573
1102	42-43	0.5194985895158339
1102	44-45	0.4560292080000252
1102	46-47	0.3290860306926575
1102	48-49	0.40964762484824035
1102	50-51	0.47495675287724026
1102	52-53	0.3764024944186133
1102	54-55	0.4020971495688599
1102	56-57	0.3794881997706838
1102	58-59	0.5163721640146122
1102	60-61	0.4638225051327183
1102	62-63	0.430281505596092
1102	64-65	0.3941063031233156
1102	66-67	0.5108595502873925
1102	68-69	0.5128246193203125
1102	70-71	0.565444554440127
1102	72-73	0.4979058609301248
1102	74-75	0.5798695275444317
1102	76-77	0.601364446174486
1102	78-79	0.5620706225806416
1102	80-81	0.4805297159795181
1102	82-83	0.5576636704352538
1102	84-85	0.6037996062994537
1102	86-87	0.6435969007371583
1102	88-89	0.5823836247071448
1102	90-91	0.6716043802573424
1102	92-93	0.6547626998756719
1102	94-95	0.6130957895837597
1102	96-97	0.5958294652696345
1102	98-99	0.61436822871228
1102	100	0.6091696226917946
1103	1	0.3449372674826776
1103	2	0.37912199875685104
1103	3	0.3721007313409679
1103	4	0.42469750695433106
1103	5	0.44114548384506236
1103	6	0.5052613481788768
1103	7	0.4720930796118594
1103	8	0.5431717397012079
1103	9	0.6278995297743322
1103	10-11	0.5818966214365133
1103	12-13	0.6239519250838867
1103	14-15	0.7251840410871111
1103	16-17	0.7657501346056819
1103	18-19	0.674476133212238
1103	20-21	0.6565061422485456
1103	22-23	0.651452746681727
1103	24-25	0.7022288372049772
1103	26-27	0.6808486744144275
1103	28-29	0.6862956131173163
1103	30-31	0.713615197650519
1103	32-33	0.7609754921846843
1103	34-35	0.7536600149728514
1103	36-37	0.70412409566827
1103	38-39	0.7484706154822689
1103	40-41	0.7323904269393253
1103	42-43	0.7494146620172515
1103	44-45	0.5841288295370504
1103	46-47	0.6096397270388323
1103	48-49	0.6354424521538604
1103	50-51	0.6048898335756263
1103	52-53	0.6168542272357485
1103	54-55	0.5942787016708451
1103	56-57	0.5063065779228566
1103	58-59	0.5514576271453677
1103	60-61	0.5582868876433977
1103	62-63	0.5147277541874118
1103	64-65	0.5315670404959505
1103	66-67	0.5433731048414856
1103	68-69	0.591107660190147
1103	70-71	0.5609432555767384
1103	72-73	0.5854794152639755
1103	74-75	0.5496271179111858
1103	76-77	0.6412353918914206
1103	78-79	0.4986821737159701
1103	80-81	0.5503248712129611
1103	82-83	0.5600503854427217
1103	84-85	0.6222447511933851
1103	86-87	0.5596942414627328
1103	88-89	0.5398188375482107
1103	90-91	0.6313508724696675
1103	92-93	0.592437936307963
1103	94-95	0.5950037789089748
1103	96-97	0.5788226142700026
1103	98-99	0.6619819419687616
1103	100	0.626824987676688
1104	1	0.11510602248818458
1104	2	0.12641344434207724
1104	3	0.2437688816481618
1104	4	0.26670005115240514
1104	5	0.3152972673963177
1104	6	0.36013536857235806
1104	7	0.40800921417884695
1104	8	0.42890499221809364
1104	9	0.5133942280949526
1104	10-11	0.5073497414042905
1104	12-13	0.45052760832647465
1104	14-15	0.5867076528223478
1104	16-17	0.6351296704367897
1104	18-19	0.5722895526695595
1104	20-21	0.5176536344843683
1104	22-23	0.6083892015820567
1104	24-25	0.6566698546562009
1104	26-27	0.5495986447257835
1104	28-29	0.5592377862880866
1104	30-31	0.5130221847898753
1104	32-33	0.5723764779426901
1104	34-35	0.5979279690024697
1104	36-37	0.5009446793517682
1104	38-39	0.5587432189911326
1104	40-41	0.6028028900897766
1104	42-43	0.530749957492894
1104	44-45	0.5002999254327918
1104	46-47	0.4287018556338822
1104	48-49	0.3431878869903784
1104	50-51	0.41117066820179815
1104	52-53	0.45624101184628785
1104	54-55	0.4299313673311076
1104	56-57	0.455265717797694
1104	58-59	0.5011710010959405
1104	60-61	0.5070425296733987
1104	62-63	0.42441905403702407
1104	64-65	0.4939615270534077
1104	66-67	0.5184852804158595
1104	68-69	0.48083413039557854
1104	70-71	0.4887899690675468
1104	72-73	0.4998716625851074
1104	74-75	0.5625216799965571
1104	76-77	0.5388057919803444
1104	78-79	0.5730919397124481
1104	80-81	0.5923472474255682
1104	82-83	0.5087640358539502
1104	84-85	0.5747062248331929
1104	86-87	0.5351233492548388
1104	88-89	0.5282724961867515
1104	90-91	0.5641207707492448
1104	92-93	0.514874880002143
1104	94-95	0.5365696150219534
1104	96-97	0.5168570929991567
1104	98-99	0.5116236594069719
1104	100	0.5721977867980925
1105	1	0.24847257298632996
1105	2	0.29593598147425126
1105	3	0.3807522393724909
1105	4	0.44648182133796865
1105	5	0.37972373477464316
1105	6	0.4574890372562308
1105	7	0.46184748137815745
1105	8	0.5212982431640683
1105	9	0.5705171308723038
1105	10-11	0.5458133962592768
1105	12-13	0.5247135863614005
1105	14-15	0.6847800501274719
1105	16-17	0.6318890741538894
1105	18-19	0.5839136376410394
1105	20-21	0.5625543052733306
1105	22-23	0.5914331573764997
1105	24-25	0.5765766140379398
1105	26-27	0.5570643581660235
1105	28-29	0.598591794834455
1105	30-31	0.6259469030482521
1105	32-33	0.6915289110359168
1105	34-35	0.72993387924069
1105	36-37	0.7050546809045883
1105	38-39	0.6984537627125391
1105	40-41	0.7363781465614494
1105	42-43	0.6720580014670006
1105	44-45	0.6862814853284362
1105	46-47	0.6498062641046616
1105	48-49	0.686750651317702
1105	50-51	0.6699155812512032
1105	52-53	0.6901808147992838
1105	54-55	0.735368691265478
1105	56-57	0.7054347792498206
1105	58-59	0.7504120328276684
1105	60-61	0.7017505537245299
1105	62-63	0.7209912037698913
1105	64-65	0.6396670457172817
1105	66-67	0.6970540014927522
1105	68-69	0.6251479707100103
1105	70-71	0.6668713280487779
1105	72-73	0.6622784659145893
1105	74-75	0.6896379008866731
1105	76-77	0.8223253721622541
1105	78-79	0.7132150885971242
1105	80-81	0.7292770438449914
1105	82-83	0.6530101234066308
1105	84-85	0.7024505321362824
1105	86-87	0.7293020777442543
1105	88-89	0.7354587020174428
1105	90-91	0.7515141974861699
1105	92-93	0.7465189172463873
1105	94-95	0.7282843861494612
1105	96-97	0.6829403076736007
1105	98-99	0.7009289958342251
1105	100	0.6749452162216905
1106	1	0.2296153961703702
1106	2	0.23020770767012166
1106	3	0.2837372228481492
1106	4	0.38343215735009295
1106	5	0.36144093917085485
1106	6	0.4591981678033221
1106	7	0.4500130387233625
1106	8	0.39565992584341103
1106	9	0.5083073639399629
1106	10-11	0.5615220806082775
1106	12-13	0.5889068161604314
1106	14-15	0.6899250303057229
1106	16-17	0.6487596790183474
1106	18-19	0.6272788639664242
1106	20-21	0.5647514510979335
1106	22-23	0.6036438437096336
1106	24-25	0.6090453530784217
1106	26-27	0.5940628894343618
1106	28-29	0.6223929717030146
1106	30-31	0.6597872703750767
1106	32-33	0.6292247944832638
1106	34-35	0.6709560281832552
1106	36-37	0.6048707185767412
1106	38-39	0.7051297783972501
1106	40-41	0.6978134249204828
1106	42-43	0.6809595000636506
1106	44-45	0.6346198993505183
1106	46-47	0.6822076692511061
1106	48-49	0.69090535467015
1106	50-51	0.6719381630672174
1106	52-53	0.7402079548889589
1106	54-55	0.7221812859371113
1106	56-57	0.6806315110331624
1106	58-59	0.7326278344936412
1106	60-61	0.7519856027574576
1106	62-63	0.7070727637700784
1106	64-65	0.7630158329787733
1106	66-67	0.77929633475609
1106	68-69	0.846976486560834
1106	70-71	0.7877971388892107
1106	72-73	0.8089111338504722
1106	74-75	0.8421479383449295
1106	76-77	0.9122872521174727
1106	78-79	0.7842298281464295
1106	80-81	0.810842240642959
1106	82-83	0.7921123325268695
1106	84-85	0.7808733435981807
1106	86-87	0.8091223117926063
1106	88-89	0.8416882464601265
1106	90-91	0.8559980352196561
1106	92-93	0.8472488903874478
1106	94-95	0.8739993077667698
1106	96-97	0.8544663073509682
1106	98-99	1.001798531351163
1106	100	1.0061518022937719
1107	1	0.20332056949692756
1107	2	0.18960260887450175
1107	3	0.1895396614189231
1107	4	0.24161715428712682
1107	5	0.21612493392153453
1107	6	0.27931127411196854
1107	7	0.2911519607921207
1107	8	0.2571309592010067
1107	9	0.3295373523702807
1107	10-11	0.3722175443672455
1107	12-13	0.3521144625331516
1107	14-15	0.26487923232470933
1107	16-17	0.3649320836170986
1107	18-19	0.34367189535114306
1107	20-21	0.2552783086934056
1107	22-23	0.30197423181890315
1107	24-25	0.33138485993274713
1107	26-27	0.3606819671029271
1107	28-29	0.41496771755107176
1107	30-31	0.3465893078439919
1107	32-33	0.37965672048625265
1107	34-35	0.39920674388155675
1107	36-37	0.40450342328256994
1107	38-39	0.34424881349887215
1107	40-41	0.35955697847747814
1107	42-43	0.3527547960006885
1107	44-45	0.32431078382501966
1107	46-47	0.3249411783004703
1107	48-49	0.3461357078100491
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2209	14-15	0.38308329724832646
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2212	60-61	-0.32580942866076157
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2212	64-65	-0.3876662619897644
2212	66-67	-0.4321212297508268
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2212	98-99	-0.41971740043458894
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2213	44-45	-0.505760700891912
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2213	86-87	-0.4693630488631477
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2213	98-99	-0.5716422025410566
2213	100	-0.538728705887312
2214	1	-0.14764598395037964
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2214	3	-0.2993953943856589
2214	4	-0.26933541158472707
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2214	16-17	-0.5363462187943995
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2214	32-33	-0.4210858120515937
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2214	86-87	-0.28127606515728587
2214	88-89	-0.23522928199711046
2214	90-91	-0.2722943737833674
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2214	94-95	-0.2874767960073257
2214	96-97	-0.34405287396235806
2214	98-99	-0.27821737758927867
2214	100	-0.2351894282042437
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2215	2	-0.5944444392200197
2215	3	-0.6099509440733542
2215	4	-0.5575650028549575
2215	5	-0.5165490127486692
2215	6	-0.6013548247688263
2215	7	-0.6331288803191697
2215	8	-0.5542342408364007
2215	9	-0.7412019279127264
2215	10-11	-0.777645376796265
2215	12-13	-0.7622986041328232
2215	14-15	-0.9458789861453596
2215	16-17	-0.8166556658863868
2215	18-19	-0.8771025678902191
2215	20-21	-0.845716365489686
2215	22-23	-0.9069743435031938
2215	24-25	-0.9072274955720232
2215	26-27	-0.9001102843640894
2215	28-29	-0.8874014697400767
2215	30-31	-0.8605942585970254
2215	32-33	-0.8743696956028089
2215	34-35	-0.9312206127021341
2215	36-37	-0.9808234045432087
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2215	40-41	-1.0362243324910665
2215	42-43	-0.88774776058181
2215	44-45	-0.8751623620597471
2215	46-47	-0.9911505450472688
2215	48-49	-0.9066714359200319
2215	50-51	-0.7987283999879722
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2215	54-55	-0.7787154791294029
2215	56-57	-0.8415705572947516
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2215	62-63	-0.6711041212031716
2215	64-65	-0.6605812266882225
2215	66-67	-0.6373841816228989
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2215	80-81	-0.7666703412802001
2215	82-83	-0.7549587542655871
2215	84-85	-0.7030706427856828
2215	86-87	-0.6338144881891878
2215	88-89	-0.6214833029482527
2215	90-91	-0.6109385653344006
2215	92-93	-0.6475249721796814
2215	94-95	-0.6359238157230713
2215	96-97	-0.6510613873945097
2215	98-99	-0.7635494214319607
2215	100	-0.6820928278190337
2216	1	-1.5776362670962136
2216	2	-1.4408891102064096
2216	3	-1.6157893536848107
2216	4	-1.7622912208605825
2216	5	-1.7208231555606375
2216	6	-3.1684553566439853
2216	7	-3.040365797803606
2216	8	-3.8696768719365764
2216	9	-3.461005853425405
2216	10-11	-2.668638115290257
2216	12-13	-2.3795323109874147
2216	14-15	-2.6443977293466574
2216	16-17	-3.0758155287294144
2216	18-19	-2.7123619231143437
2216	20-21	-2.494107511224101
2216	22-23	-2.730176748289267
2216	24-25	-2.7766693188822416
2216	26-27	-2.8134530650543397
2216	28-29	-3.265611320802094
2216	30-31	-3.0088960454599842
2216	32-33	-3.0344165668823138
2216	34-35	-3.9483382064255608
2216	36-37	-3.0512266842155285
2216	38-39	-2.963708809092296
2216	40-41	-3.537864358524999
2216	42-43	-3.3867520855867923
2216	44-45	-3.2232609435262347
2216	46-47	-3.0750701253857358
2216	48-49	-3.2217717620205732
2216	50-51	-3.5003482401732526
2216	52-53	-3.874256559078141
2216	54-55	-3.2238216005028377
2216	56-57	-3.232837632270943
2216	58-59	-4.191184873709467
2216	60-61	-4.91629352335681
2216	62-63	-4.027856361746338
2216	64-65	-4.033676587734554
2216	66-67	-3.7068536432879142
2216	68-69	-3.9719241550454285
2216	70-71	-3.7361128242569244
2216	72-73	-4.160643718209052
2216	74-75	-4.617327667845004
2216	76-77	-5.242390438558591
2216	78-79	-4.239468628439639
2216	80-81	-4.094134084333451
2216	82-83	-4.31022576254896
2216	84-85	-4.332096635240255
2216	86-87	-4.011687493093664
2216	88-89	-4.029127052505832
2216	90-91	-4.197698909291294
2216	92-93	-4.289000328682118
2216	94-95	-4.127627354791262
2216	96-97	-4.2834112213240765
2216	98-99	-4.094284227264215
2216	100	-3.773357250509992
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79164.0
3	14234.0
4	9257.0
5	7543.0
6	6946.0
7	7033.0
8	7605.0
9	8627.0
10	9656.0
11	10835.0
12	11990.0
13	12958.0
14	14541.0
15	15479.0
16	15816.0
17	16438.0
18	15629.0
19	14724.0
20	14617.0
21	15514.0
22	17163.0
23	20392.0
24	24962.0
25	30778.0
26	37391.0
27	43975.0
28	53528.0
29	66358.0
30	79603.0
31	95467.0
32	120480.0
33	159008.0
34	218079.0
35	315481.0
36	512119.0
37	858652.0
38	1040131.0
39	336455.0
40	701.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.87889620102395	19.84859778017777	13.667270044568838	23.605235974229448
2	15.661274186632982	20.00064543790657	40.11610966912087	24.221970706339583
3	18.542961784085524	24.672182768076627	30.042866811369436	26.741988636468417
4	12.613681734481515	16.37003145236916	38.48881094184677	32.52747587130255
5	13.204202339904686	37.71012909795109	35.42257730696801	13.663091255176216
6	31.637974080079818	37.44787130500053	17.357904496772804	13.556250118146847
7	27.473274529190718	32.255769665665646	22.184013796527953	18.086942008615683
8	25.026740951184856	36.450014246472264	20.27810128181366	18.245143520529215
9	24.84726515764504	16.909225871445006	21.324070808292472	36.91943816261748
10-11	24.055742723974205	26.87466854591981	28.75276905714721	20.316819672958776
12-13	24.310206311644812	24.33611773560896	28.837651327672315	22.516024625073914
14-15	21.74356354639989	26.58500904468028	25.877225640279683	25.794201768640146
16-17	21.454654720833854	29.92755230143849	26.515700920188124	22.102092057539537
18-19	21.397973387995357	28.98503767753717	28.428470310547926	21.188518623919546
20-21	22.340192923302446	28.220239945605535	28.09055862094959	21.349008510142426
22-23	22.07901962004744	27.988835637438303	28.15539049911132	21.77675424340293
24-25	21.918048212845786	28.1291508049536	28.091495324819142	21.861305657381465
26-27	21.66525780546374	28.1269745475988	28.172110357285202	22.035657289652256
28-29	21.736931456747126	28.166428018917085	28.24456340140528	21.852077122930506
30-31	21.918147528087623	28.463521390967582	27.866378130411857	21.75195295053294
32-33	21.610376862870122	28.242965589879383	28.264323373206572	21.88233417404392
34-35	21.793344445978686	27.967513147184615	28.286150694755484	21.95299171208121
36-37	21.988425997695526	28.324701835568213	27.849077167583985	21.837794999152276
38-39	21.90611438041722	28.460470902253572	27.9274351201506	21.70597959717861
40-41	21.78850871738365	28.163226258939115	28.11156683538774	21.936698188289494
42-43	21.71994073819991	28.212667059931917	28.072476987874012	21.994915213994158
44-45	21.805612992666337	28.250275813390168	28.033843559290545	21.91026763465295
46-47	21.82026818800384	28.334516835486205	27.912605329482503	21.932609647027444
48-49	21.826318794663756	28.24786099196678	27.897846734602567	22.027973478766903
50-51	21.879948562325765	28.199781676878782	27.837073921544057	22.083195839251392
52-53	21.84042223599186	28.188542404325634	27.907321449647398	22.063713910035112
54-55	21.952401896510708	28.461929631412296	27.726337875443285	21.85933059663371
56-57	21.817425102748846	28.27018760287419	27.77228833691226	22.1400989574647
58-59	21.723838737807746	27.97440494151476	28.04845804721557	22.253298273461926
60-61	21.932106757317314	28.253878206905085	27.791892316474915	22.022122719302683
62-63	21.77668682750154	28.308027405457764	27.79770195066335	22.117583816377348
64-65	21.820113274451334	28.05932473065882	27.934895946486865	22.185666048402982
66-67	21.696244327119366	28.090486710568292	27.829478878719762	22.38379008359258
68-69	22.03009698275241	28.20564331392987	27.752707524015175	22.011552179302544
70-71	21.99934395136296	28.03341237728191	27.82440348789203	22.142840183463104
72-73	21.794732307136247	28.194180191036967	27.90137563395846	22.109711867868327
74-75	21.849277902612055	28.172090426527525	27.86583409324982	22.112797577610603
76-77	21.909747878684776	28.171109957782885	27.652492600853883	22.266649562678452
78-79	21.97052611876133	28.232002795664325	27.737586517374012	22.05988456820033
80-81	21.90287301336481	28.082702620278642	27.827132584649362	22.187291781707184
82-83	21.815621689659466	28.11426291354801	27.926904338671832	22.14321105812069
84-85	21.85484420293906	28.00980461875595	27.852145593079275	22.283205585225716
86-87	21.94943387623479	28.202572461041207	27.722654966418226	22.12533869630578
88-89	21.806378011872876	28.0842889885787	27.805758389054436	22.303574610493985
90-91	21.803602996788232	27.962516404029316	27.85899208203755	22.374888517144907
92-93	21.856673188245026	28.149441151639664	27.71655049805073	22.277335162064585
94-95	21.80776673739631	27.96215637436997	27.827005508152737	22.40307138008098
96-97	21.82878965849328	27.95667256389179	27.82327636369586	22.391261413919064
98-99	21.824422623866504	28.058324224782215	27.757551916436853	22.359701234914432
100	21.869809825436146	27.91058248867509	27.766919724224643	22.452687961664118
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1545.0
1	1280.0
2	1186.5
3	1353.0
4	1214.5
5	968.5
6	768.0
7	686.0
8	694.5
9	693.5
10	626.0
11	578.0
12	623.0
13	679.0
14	748.0
15	861.0
16	1052.5
17	1343.5
18	1802.5
19	2522.5
20	3731.0
21	5547.0
22	8051.5
23	11612.5
24	16802.0
25	23480.0
26	31554.0
27	41129.5
28	52095.0
29	64633.5
30	77364.5
31	88733.0
32	98423.5
33	107023.5
34	115733.5
35	123122.0
36	129884.5
37	137303.0
38	143833.5
39	148137.0
40	150622.5
41	152690.5
42	151960.5
43	150160.5
44	148944.0
45	146788.5
46	144495.5
47	140179.0
48	135189.0
49	131945.0
50	129538.5
51	125894.5
52	118951.0
53	112300.5
54	107678.5
55	103297.0
56	96891.0
57	89098.5
58	81973.5
59	75235.0
60	67458.0
61	58122.0
62	50443.5
63	43505.5
64	36026.5
65	29710.5
66	24372.5
67	19467.0
68	15442.5
69	12272.5
70	9477.5
71	7256.5
72	5721.5
73	4579.5
74	3548.0
75	2655.0
76	1950.5
77	1398.0
78	1008.5
79	703.0
80	482.5
81	318.5
82	215.5
83	152.0
84	105.0
85	79.0
86	71.0
87	63.0
88	48.0
89	34.5
90	23.5
91	16.5
92	15.0
93	15.5
94	13.5
95	11.0
96	9.0
97	10.0
98	10.5
99	7.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00974805090833168
2	0.02740054971632711
3	0.028598891671961266
4	0.030903395432796178
5	0.03364575490818972
6	0.034728871675782126
7	0.03286222362950585
8	0.032562638140597316
9	0.03270090836624741
10-11	0.033081151486785164
12-13	0.03396838543470661
14-15	0.032723953403855754
16-17	0.030027684003678907
18-19	0.0322515301328846
20-21	0.03197498968158441
22-23	0.03270090836624741
24-25	0.0303042244549791
26-27	0.030188999266937352
28-29	0.031007098102033746
30-31	0.03091491795160035
32-33	0.030695990094321033
34-35	0.030603809943887637
36-37	0.028679549303590485
38-39	0.02886390960445728
40-41	0.029993116447266387
42-43	0.029728098514770374
44-45	0.028829342048044757
46-47	0.028011243212948362
48-49	0.02811494588218593
50-51	0.029117405018149117
52-53	0.02952069317629523
54-55	0.028967612273694848
56-57	0.027711657724039822
58-59	0.02825321610783603
60-61	0.027872972987298267
62-63	0.02799972069414419
64-65	0.02545324403842161
66-67	0.026386568061559746
68-69	0.02734293712230624
70-71	0.02848366648391952
72-73	0.02560303678287588
74-75	0.02611002761025956
76-77	0.02672072110688081
78-79	0.027262279490677017
80-81	0.02727380200948119
82-83	0.02589109975298024
84-85	0.026951171482964303
86-87	0.027365982159914586
88-89	0.027792315355669044
90-91	0.028068855806969238
92-93	0.026882036370139254
94-95	0.027976675656535836
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4339329.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.73012827391615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.30607435430832	52.92104514563726
2	12.781863927501558	17.058708389238408
3	3.7166470767399256	7.44037008539192
4	1.5189920619135042	4.054501405541941
5	0.7824671470248478	2.6107066545546647
6	0.4703506203493953	1.8831934337778713
7	0.30271964780893357	1.4140364650527397
8	0.20512136513975693	1.0950220005997418
9	0.1507516215737241	0.9053707540603926
>10	0.7251887902173547	8.342424011616757
>50	0.032414840633544786	1.4474661454980478
>100	0.007270225364798896	0.7417069041652355
>500	1.0374109674535345E-4	0.0447665038222561
>1k	3.458032750934893E-5	0.04068210104268437
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.3045037608349124E-5	0.0
4	0.0	0.0	0.0	2.3045037608349124E-5	0.0
5	0.0	0.0	0.0	2.3045037608349124E-5	0.0
6	0.0	0.0	0.0	2.3045037608349124E-5	0.0
7	0.0	0.0	0.0	2.3045037608349124E-5	0.0
8	0.0	0.0	0.0	2.3045037608349124E-5	0.0
9	0.0	0.0	0.0	2.3045037608349124E-5	0.0
10-11	0.0	3.4567556412523684E-5	0.0	2.3045037608349124E-5	0.0
12-13	0.0	4.609007521669825E-5	0.0	4.609007521669825E-5	0.0
14-15	0.0	5.761259402087282E-5	0.0	4.609007521669825E-5	0.0
16-17	0.0	1.1522518804174564E-4	0.0	6.913511282504738E-5	0.0
18-19	0.0	1.6131526325844386E-4	0.0	1.2674770684592017E-4	0.0
20-21	0.0	1.7283778206261843E-4	0.0	1.9588281967096755E-4	0.0
22-23	2.3045037608349124E-5	1.84360300866793E-4	0.0	3.341530453210623E-4	0.0
24-25	2.3045037608349124E-5	1.84360300866793E-4	0.0	5.300358649920299E-4	0.0
26-27	2.3045037608349124E-5	1.84360300866793E-4	0.0	8.872339479214413E-4	0.0
28-29	2.3045037608349124E-5	2.0740533847514212E-4	0.0	0.00237363887365996	0.0
30-31	2.3045037608349124E-5	2.765404513001895E-4	0.0	0.007155484177392403	0.0
32-33	2.3045037608349124E-5	2.765404513001895E-4	0.0	0.017514228582345335	0.0
34-35	2.3045037608349124E-5	2.765404513001895E-4	0.0	0.03426797092361515	0.0
36-37	2.3045037608349124E-5	3.226305265168878E-4	0.0	0.06136893515103372	0.0
38-39	2.3045037608349124E-5	3.917656393419351E-4	0.0	0.09642043735333275	0.0
40-41	2.3045037608349124E-5	4.0328815814610967E-4	0.0	0.13333858760190803	0.0
42-43	2.3045037608349124E-5	4.1481067695028423E-4	0.0	0.16948472909060364	0.0
44-45	2.3045037608349124E-5	4.1481067695028423E-4	0.0	0.2142151470884093	0.0
46-47	2.3045037608349124E-5	4.3785571455863336E-4	0.0	0.2667578328354453	0.0
48-49	2.3045037608349124E-5	4.3785571455863336E-4	0.0	0.32031450023724867	0.0
50-51	2.3045037608349124E-5	4.609007521669825E-4	0.0	0.3768900675657457	0.0
52-53	3.4567556412523684E-5	4.839457897753316E-4	0.0	0.4311496086146038	0.0
54-55	4.609007521669825E-5	5.069908273836808E-4	0.0	0.49195394034423295	0.0
56-57	4.609007521669825E-5	5.069908273836808E-4	0.0	0.5637392324942405	0.0
58-59	4.609007521669825E-5	5.300358649920299E-4	0.0	0.6404331176548264	0.0
60-61	6.913511282504738E-5	5.300358649920299E-4	0.0	0.7189129932300593	0.0
62-63	6.913511282504738E-5	5.415583837962045E-4	0.0	0.7941550410213192	0.0
64-65	6.913511282504738E-5	5.53080902600379E-4	0.0	0.8758266543053086	0.0
66-67	6.913511282504738E-5	5.646034214045535E-4	0.0	0.9678570119942507	0.0
68-69	6.913511282504738E-5	6.106934966212518E-4	0.0	1.0675959347631858	0.0
70-71	6.913511282504738E-5	6.5678357183795E-4	0.0	1.1711027211810858	0.0
72-73	6.913511282504738E-5	6.913511282504737E-4	0.0	1.278250623541105	0.0
74-75	6.913511282504738E-5	6.913511282504737E-4	0.0	1.3874380117294631	0.0
76-77	6.913511282504738E-5	7.143961658588228E-4	0.0	1.505981685186811	0.0
78-79	6.913511282504738E-5	7.143961658588228E-4	0.0	1.632303058836977	0.0
80-81	6.913511282504738E-5	7.489637222713465E-4	0.0	1.7726012477966062	0.0
82-83	6.913511282504738E-5	7.604862410755211E-4	0.0	1.915342210742721	0.0
84-85	6.913511282504738E-5	7.604862410755211E-4	0.0	2.0587399572606735	0.0
86-87	6.913511282504738E-5	8.641889103130922E-4	0.0	2.2131762767930248	0.0
88	6.913511282504738E-5	8.757114291172667E-4	0.0	2.3347618952146747	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3885	0.0	40.533283	1
GTATCAA	6280	0.0	34.955482	1
TCAACGC	6495	0.0	32.422527	4
ATCAACG	6720	0.0	31.406902	3
CAACGCA	7045	0.0	29.82494	5
AACGCAG	7390	0.0	28.557482	6
TATCAAC	7895	0.0	27.20897	2
GTACATG	10705	0.0	25.073069	1
ACGCAGA	8540	0.0	24.271336	7
TACATGG	10795	0.0	23.949038	2
CGCAGAG	8915	0.0	23.408556	8
ACATGGG	11105	0.0	22.095304	3
GCAGAGT	10345	0.0	19.309517	9
CATGGGG	8635	0.0	19.215885	4
GAGTACA	8120	0.0	18.582624	1
AGAGTAC	8805	0.0	17.21525	10-11
TACGTCG	255	6.0897237E-6	16.590342	5
CAGAGTA	9835	0.0	16.511505	10-11
AGTACAT	8545	0.0	16.282747	2
AGTACCG	555	0.0	16.092134	5
>>END_MODULE