FastQCFastQC Report
Wed 25 May 2016
SRR2049341_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049341_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4339329
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104650.24116631857137358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99200.22860677307482333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57230.13188675023258206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68050.057.2082371
GTATCAA122100.040.5479621
TCAACGC151850.031.8461344
ATCAACG152900.031.688913
CAACGCA157700.030.6945785
TATCAAC161750.030.013192
AACGCAG163250.029.621816
GTGGTAT28350.029.3546261
ACGCAGA189000.025.3374377
GTACATG110100.025.3234981
CGCAGAG193600.024.613928
TGGTATC32350.024.260422
TACATGG110400.024.2214052
ACATGGG114000.022.4671383
GCAGAGT209900.022.2542769
AGAGTAC191950.020.84312810-11
GAGTACT151850.020.73025512-13
CAGAGTA203300.020.36207210-11
CATGGGG87850.019.3117454
AGTACTT160350.019.05957612-13
Produced by FastQC (version 0.11.5)