##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049341_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4339329
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85901230351513	33.0	31.0	34.0	30.0	34.0
2	31.96217525797191	33.0	31.0	34.0	30.0	34.0
3	31.947815434137397	33.0	31.0	34.0	30.0	34.0
4	35.66839550538805	37.0	35.0	37.0	33.0	37.0
5	35.752955353235485	37.0	35.0	37.0	35.0	37.0
6	35.80839341750764	37.0	35.0	37.0	35.0	37.0
7	35.74997148176596	37.0	35.0	37.0	33.0	37.0
8	35.73907371393135	37.0	35.0	37.0	33.0	37.0
9	37.44115922069979	39.0	37.0	39.0	34.0	39.0
10-11	37.416309871871896	39.0	37.0	39.0	34.0	39.0
12-13	37.37107776340535	39.0	37.0	39.0	34.0	39.0
14-15	38.65884633315427	40.0	38.0	41.0	34.0	41.0
16-17	38.68260161882171	40.0	38.0	41.0	34.0	41.0
18-19	38.7493861378107	40.0	38.0	41.0	34.0	41.0
20-21	38.77878261823429	40.0	38.0	41.0	34.0	41.0
22-23	38.747376149630504	40.0	38.0	41.0	34.0	41.0
24-25	38.730656974845644	40.0	38.0	41.0	34.0	41.0
26-27	38.612759599468035	40.0	38.0	41.0	34.0	41.0
28-29	38.529650667188406	40.0	38.0	41.0	34.0	41.0
30-31	38.42577297089019	40.0	38.0	41.0	34.0	41.0
32-33	38.29216855877948	40.0	38.0	41.0	34.0	41.0
34-35	38.193434053974705	40.0	38.0	41.0	34.0	41.0
36-37	38.08387402752822	40.0	38.0	41.0	33.5	41.0
38-39	37.98830118665812	40.0	37.5	41.0	33.0	41.0
40-41	37.874710813584315	40.0	37.0	41.0	33.0	41.0
42-43	37.73717480283242	40.0	37.0	41.0	33.0	41.0
44-45	37.559064316165006	40.0	37.0	41.0	33.0	41.0
46-47	37.332012737453184	40.0	37.0	41.0	32.0	41.0
48-49	37.12224943072996	40.0	37.0	41.0	31.5	41.0
50-51	36.97106949023686	40.0	36.0	41.0	31.0	41.0
52-53	36.82733275121568	40.0	36.0	41.0	31.0	41.0
54-55	36.68454685044624	39.0	36.0	41.0	31.0	41.0
56-57	36.53390973581399	39.0	35.0	41.0	31.0	41.0
58-59	36.33794948481666	39.0	35.0	41.0	30.5	41.0
60-61	36.15643755059826	39.0	35.0	41.0	30.0	41.0
62-63	35.95071150401364	38.5	35.0	40.5	30.0	41.0
64-65	35.72351543291602	38.0	35.0	40.0	30.0	41.0
66-67	35.45483172628764	37.5	35.0	40.0	29.0	41.0
68-69	35.16967473542569	37.0	34.5	40.0	29.0	41.0
70-71	34.84542206871154	36.5	34.0	39.0	29.0	41.0
72-73	34.55258819969632	36.0	34.0	39.0	29.0	41.0
74-75	34.25218680123125	36.0	34.0	39.0	29.0	40.5
76-77	33.93126875145904	35.0	34.0	38.0	28.5	40.0
78-79	33.60343062256861	35.0	34.0	37.0	28.0	39.0
80-81	33.29448400893318	35.0	34.0	37.0	28.0	39.0
82-83	33.00208592157912	35.0	33.5	36.0	27.5	39.0
84-85	32.73967507418774	35.0	33.0	36.0	27.0	37.0
86-87	32.51147435467557	35.0	33.0	36.0	27.0	37.0
88-89	32.288215643478516	35.0	33.0	35.0	26.5	37.0
90-91	32.1016933724085	35.0	33.0	35.0	26.0	36.0
92-93	31.92279439977932	35.0	33.0	35.0	26.0	36.0
94-95	31.767460245581745	35.0	33.0	35.0	26.0	36.0
96-97	31.566833028793162	35.0	33.0	35.0	25.0	36.0
98-99	31.415283215446443	35.0	33.0	35.0	25.0	35.0
100	31.297445987616978	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20063260286182683
1101	2	0.20889894575143586
1101	3	0.38436227917545907
1101	4	0.2474118311454916
1101	5	0.17129829363893379
1101	6	0.1715427259826896
1101	7	0.052069691962195463
1101	8	0.13274369343407955
1101	9	0.2843196075543659
1101	10-11	0.25496532794303306
1101	12-13	0.14125264504080803
1101	14-15	0.31840417762530393
1101	16-17	0.22480107571686858
1101	18-19	0.29985773584954245
1101	20-21	0.2770390748129756
1101	22-23	0.2571099118964497
1101	24-25	0.2546972887004344
1101	26-27	0.36098469966157154
1101	28-29	0.31719320052973643
1101	30-31	0.2822749653416423
1101	32-33	0.33753645156760115
1101	34-35	0.3064945129094383
1101	36-37	0.2871342140470219
1101	38-39	0.3183350846409425
1101	40-41	0.3254425069803517
1101	42-43	0.3041694436821416
1101	44-45	0.37205261019273195
1101	46-47	0.3187415281362007
1101	48-49	0.3501152342142646
1101	50-51	0.32180328047936513
1101	52-53	0.3717609492161813
1101	54-55	0.3513355350977818
1101	56-57	0.3503591068195462
1101	58-59	0.3158442759769926
1101	60-61	0.358563553659792
1101	62-63	0.3074265287080209
1101	64-65	0.2279896154418779
1101	66-67	0.1724174199689017
1101	68-69	0.2488302479694724
1101	70-71	0.24640024540418182
1101	72-73	0.21683915141344556
1101	74-75	0.34904254433030246
1101	76-77	0.28544255812821007
1101	78-79	0.2816493134951372
1101	80-81	0.27949966606664134
1101	82-83	0.2740023872310502
1101	84-85	0.29266764170871085
1101	86-87	0.1719835540481469
1101	88-89	0.3346000868898926
1101	90-91	0.2997836989466265
1101	92-93	0.3212601869327507
1101	94-95	0.3527211686775722
1101	96-97	0.3380292538021479
1101	98-99	0.3467690039330158
1101	100	0.3336377273803741
1102	1	0.1693660641451693
1102	2	0.1791199879346692
1102	3	0.3523041053254623
1102	4	0.1795542985362104
1102	5	0.15652020670720646
1102	6	0.17877006813938578
1102	7	0.12639827517135416
1102	8	0.15734658319706085
1102	9	0.09553792807894013
1102	10-11	0.18319849340495864
1102	12-13	0.22505093104250307
1102	14-15	0.23143677210172342
1102	16-17	0.2213960486203348
1102	18-19	0.21199293125492602
1102	20-21	0.18166008864003658
1102	22-23	0.1965922568144407
1102	24-25	0.19765259101823318
1102	26-27	0.31067151771443946
1102	28-29	0.21630658747469766
1102	30-31	0.2031015552975859
1102	32-33	0.20574738182907737
1102	34-35	0.23704523621407958
1102	36-37	0.19903127861181247
1102	38-39	0.19141633942176384
1102	40-41	0.17321979366339235
1102	42-43	0.21280528199253013
1102	44-45	0.15690041747444639
1102	46-47	0.12006804294574636
1102	48-49	0.15113905453981857
1102	50-51	0.22356650824483637
1102	52-53	0.2646663042761972
1102	54-55	0.1591813009131826
1102	56-57	0.24347724748825073
1102	58-59	0.21544622243998646
1102	60-61	0.19360922525952873
1102	62-63	0.27771236321343906
1102	64-65	0.21339387443178026
1102	66-67	0.1618198988215127
1102	68-69	0.19841158158094174
1102	70-71	0.21993936592843966
1102	72-73	0.2274332169703115
1102	74-75	0.16364757223990978
1102	76-77	0.1599832536140795
1102	78-79	0.28919690758471006
1102	80-81	0.23837047817995938
1102	82-83	0.2619581805730178
1102	84-85	0.3151626085521926
1102	86-87	0.35661617459394535
1102	88-89	0.33520321447880974
1102	90-91	0.31483230017566655
1102	92-93	0.34471320354257884
1102	94-95	0.350041980746429
1102	96-97	0.40494008165364903
1102	98-99	0.37281551745180863
1102	100	0.33383240925197555
1103	1	0.2132839206718522
1103	2	0.18933677514744574
1103	3	0.3558079162628367
1103	4	0.23025402065408684
1103	5	0.1639390843375068
1103	6	0.13939437909327523
1103	7	0.1560104026276008
1103	8	0.18278619614433467
1103	9	0.18701559778464372
1103	10-11	0.2402205538929607
1103	12-13	0.23614064255166767
1103	14-15	0.26916003527726673
1103	16-17	0.2773686788829366
1103	18-19	0.2884281355307081
1103	20-21	0.2928156916173279
1103	22-23	0.3170961277087656
1103	24-25	0.3005658871456305
1103	26-27	0.2719547433495819
1103	28-29	0.3288914908032794
1103	30-31	0.30650711024813404
1103	32-33	0.2573378929682235
1103	34-35	0.2687427643969045
1103	36-37	0.29058407805090525
1103	38-39	0.3123981528871127
1103	40-41	0.3354645330525372
1103	42-43	0.3170606349860279
1103	44-45	0.28620046287042555
1103	46-47	0.2544479106244282
1103	48-49	0.3299061560371541
1103	50-51	0.23724801201416312
1103	52-53	0.23844682267136363
1103	54-55	0.28158127986706205
1103	56-57	0.2087082714184163
1103	58-59	0.30201488599949045
1103	60-61	0.2603290170498269
1103	62-63	0.21151083198627418
1103	64-65	0.25120193386992895
1103	66-67	0.27263937577662745
1103	68-69	0.3072098362218014
1103	70-71	0.2906962184575903
1103	72-73	0.2500952934631542
1103	74-75	0.22770270571754736
1103	76-77	0.23549377483578127
1103	78-79	0.2318429425334969
1103	80-81	0.28231861908614064
1103	82-83	0.25476463096230617
1103	84-85	0.23307425892239308
1103	86-87	0.23929357070016266
1103	88-89	0.22059109314061942
1103	90-91	0.20757004479086305
1103	92-93	0.2561702431618933
1103	94-95	0.18940231347080783
1103	96-97	0.3247674790446631
1103	98-99	0.31073859635148793
1103	100	0.3716762593204379
1104	1	0.14130990607359095
1104	2	0.17406588645468446
1104	3	0.3060573008766134
1104	4	0.19945421332151625
1104	5	0.1223494735580104
1104	6	0.17221776443477665
1104	7	0.17163418242431305
1104	8	0.17956671203322117
1104	9	0.20473335330412112
1104	10-11	0.18591750551044584
1104	12-13	0.17900475121305703
1104	14-15	0.19031621084783268
1104	16-17	0.21548070435152056
1104	18-19	0.2188465744115291
1104	20-21	0.22659593009552026
1104	22-23	0.265711781842235
1104	24-25	0.23952465650618393
1104	26-27	0.2497366168408064
1104	28-29	0.27260110830903983
1104	30-31	0.2567062379245044
1104	32-33	0.2896677817854396
1104	34-35	0.27795709835947946
1104	36-37	0.28124897068251187
1104	38-39	0.22035081446222193
1104	40-41	0.2530947664480365
1104	42-43	0.30059768783941365
1104	44-45	0.2986221917280716
1104	46-47	0.3404224961947051
1104	48-49	0.337449755592921
1104	50-51	0.2099921025488598
1104	52-53	0.19579618723936676
1104	54-55	0.2063654790479461
1104	56-57	0.24394771864911036
1104	58-59	0.274219070720811
1104	60-61	0.24284582557279322
1104	62-63	0.2830708681256624
1104	64-65	0.23850469439134514
1104	66-67	0.2998912950881092
1104	68-69	0.2298594456142382
1104	70-71	0.2610278334913474
1104	72-73	0.2796657771552944
1104	74-75	0.2708085986997233
1104	76-77	0.2464000765237131
1104	78-79	0.2505801348522567
1104	80-81	0.2930878542401558
1104	82-83	0.34197523793120865
1104	84-85	0.3334026390814486
1104	86-87	0.27564757714711874
1104	88-89	0.27132722901865947
1104	90-91	0.2476374593896935
1104	92-93	0.3781210056137816
1104	94-95	0.38152289971940334
1104	96-97	0.4088203542830051
1104	98-99	0.4383411979317806
1104	100	0.4159823573205159
1105	1	0.23276175986240233
1105	2	0.20268309653512517
1105	3	0.30254761626022386
1105	4	0.24077882009913765
1105	5	0.16616243784754658
1105	6	0.16519720495078616
1105	7	0.15468903359529662
1105	8	0.11643294903750956
1105	9	0.20428462859421614
1105	10-11	0.18519616749228618
1105	12-13	0.20393236562733108
1105	14-15	0.21908206499408323
1105	16-17	0.2223224514791653
1105	18-19	0.17092207039836893
1105	20-21	0.1875695153079917
1105	22-23	0.19376201117617597
1105	24-25	0.21571354351508631
1105	26-27	0.2180979998175019
1105	28-29	0.24234762325346537
1105	30-31	0.22660290763821678
1105	32-33	0.2554675152424153
1105	34-35	0.2666243502635126
1105	36-37	0.25318193133903577
1105	38-39	0.23583268675302094
1105	40-41	0.2554991196911587
1105	42-43	0.2888838762375059
1105	44-45	0.29212538855973236
1105	46-47	0.29384567701472974
1105	48-49	0.2913294435346856
1105	50-51	0.2606761124576096
1105	52-53	0.25659389910041597
1105	54-55	0.2130414150248825
1105	56-57	0.2270025240412039
1105	58-59	0.3175570789947102
1105	60-61	0.2259465929735356
1105	62-63	0.2004516855242784
1105	64-65	0.24175028057359071
1105	66-67	0.29633418353850516
1105	68-69	0.2400144401280926
1105	70-71	0.27850131652532184
1105	72-73	0.29939931836143074
1105	74-75	0.31316139941577603
1105	76-77	0.22355709556333636
1105	78-79	0.2435161311987386
1105	80-81	0.29334636764934174
1105	82-83	0.2676712419099445
1105	84-85	0.28142958710153465
1105	86-87	0.25482793170281326
1105	88-89	0.28091058249470535
1105	90-91	0.2899157778467156
1105	92-93	0.31350716522610256
1105	94-95	0.24146196435041745
1105	96-97	0.31996182900792647
1105	98-99	0.33985486787548425
1105	100	0.38730462754343264
1106	1	0.13473846713193538
1106	2	0.14290803171514455
1106	3	0.2857690625140954
1106	4	0.15048073703646736
1106	5	0.15462586772596154
1106	6	0.11761699233912992
1106	7	0.16883608016015472
1106	8	0.13479553708235414
1106	9	0.17283403710415257
1106	10-11	0.15938326839683015
1106	12-13	0.16714782822539576
1106	14-15	0.24674939993632705
1106	16-17	0.24247607224828016
1106	18-19	0.255928280129055
1106	20-21	0.2610374338165613
1106	22-23	0.24564330951578484
1106	24-25	0.25341809242596725
1106	26-27	0.34416319448352084
1106	28-29	0.2695951651221762
1106	30-31	0.33192498794996084
1106	32-33	0.33086800756154844
1106	34-35	0.341623004473945
1106	36-37	0.3763013791797789
1106	38-39	0.4309881196744314
1106	40-41	0.44290234942623385
1106	42-43	0.4418072998337905
1106	44-45	0.4779352789493956
1106	46-47	0.6094870074827412
1106	48-49	0.5476850125096391
1106	50-51	0.5750526450143454
1106	52-53	0.5606914752401337
1106	54-55	0.5381570596554184
1106	56-57	0.560482395204275
1106	58-59	0.596070085007149
1106	60-61	0.5504151815343121
1106	62-63	0.5414397633388148
1106	64-65	0.5758104180022414
1106	66-67	0.592303979046001
1106	68-69	0.5728249253300959
1106	70-71	0.6451960959810634
1106	72-73	0.6502841239263759
1106	74-75	0.6221027631421023
1106	76-77	0.5967923901251382
1106	78-79	0.5969685633558441
1106	80-81	0.61158448348354
1106	82-83	0.6040606501640511
1106	84-85	0.5653502576476228
1106	86-87	0.5855786260058622
1106	88-89	0.6065142424027528
1106	90-91	0.6363972865203493
1106	92-93	0.6731243129658999
1106	94-95	0.6510391736749703
1106	96-97	0.7426318386514197
1106	98-99	0.7412208842637362
1106	100	0.7929905396227817
1107	1	0.1053824492675055
1107	2	0.085460611062814
1107	3	0.19947430709519764
1107	4	0.13576940677126004
1107	5	0.10885778739472585
1107	6	0.12073844388764599
1107	7	0.0778918786065006
1107	8	0.0763366516838957
1107	9	0.10975123480474736
1107	10-11	0.16345100645929023
1107	12-13	0.13246127137129804
1107	14-15	0.19427334949414643
1107	16-17	0.1367273193830414
1107	18-19	0.13587713466971252
1107	20-21	0.12318684973370608
1107	22-23	0.08903679297340261
1107	24-25	0.12039257724635632
1107	26-27	0.1700985398191932
1107	28-29	0.18397334981486324
1107	30-31	0.14466940222246194
1107	32-33	0.14808841999375488
1107	34-35	0.1600753882235324
1107	36-37	0.16512800510400893
1107	38-39	0.16191865084795154
1107	40-41	0.1647939253276931
1107	42-43	0.18554158326261927
1107	44-45	0.13577989775957633
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2216	88-89	-1.0617926910060262
2216	90-91	-0.8567586431188907
2216	92-93	-0.9064680996897216
2216	94-95	-0.8585404178927725
2216	96-97	-0.9331584227335128
2216	98-99	-1.0734583533535123
2216	100	-1.068346183120294
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	220.0
3	32.0
4	30.0
5	72.0
6	205.0
7	597.0
8	1419.0
9	2379.0
10	3496.0
11	4850.0
12	6828.0
13	9485.0
14	11495.0
15	14711.0
16	18028.0
17	24238.0
18	23999.0
19	13696.0
20	10684.0
21	10588.0
22	11460.0
23	12732.0
24	15003.0
25	18928.0
26	24258.0
27	31663.0
28	41883.0
29	54985.0
30	71879.0
31	92080.0
32	118903.0
33	154954.0
34	210729.0
35	304893.0
36	494855.0
37	879184.0
38	1183285.0
39	459019.0
40	1584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.230705108758094	20.067710432090855	14.122784288376543	23.578800170774507
2	15.861184989660842	20.34086376027261	39.35576214663604	24.442189103430508
3	18.211778825712454	24.878754295883073	30.534628740987372	26.3748381374171
4	12.660773890126665	16.919640017661738	37.801049195681166	32.61853689653043
5	12.847954142218763	37.553778475888784	35.513993062060976	14.084274319831477
6	31.386972501969773	37.824557667786884	16.828984389061073	13.959485441182265
7	26.89897447278139	32.7381491470225	22.027944873504637	18.334931506691472
8	25.236511929529907	36.14498229674907	19.955725741107585	18.662780032613433
9	24.807545129673276	16.312637276408402	21.33424315141811	37.54557444250021
10-11	24.101445177353455	26.778783079135042	28.402536429019325	20.71723531449217
12-13	24.618565856497117	24.256463365768706	28.896336692145564	22.228634085588617
14-15	21.79172378001735	26.245366789850216	26.510454162906484	25.452455267225954
16-17	20.953976985842743	29.619072441845272	27.198997817404486	22.2279527549075
18-19	20.854876607622195	27.98449437332232	30.067846910858087	21.092782108197397
20-21	21.796922953543334	27.231491268166568	29.993154462901877	20.978431315388217
22-23	21.475417051806858	27.023602036167343	30.125245170393853	21.375735741631942
24-25	21.389366017683166	27.148349933267262	30.01368070076345	21.44860334828612
26-27	21.155158392073243	27.207836606920843	30.085898112771865	21.551106888234052
28-29	21.1777155662321	27.208216686685184	30.189585178862288	21.42448256822043
30-31	21.414758392861565	27.50248742460669	29.801357468409563	21.281396714122177
32-33	21.195093330334398	27.255642476536636	30.194883159058634	21.35438103407033
34-35	21.299697683360623	27.027755126402734	30.18595405805673	21.486593132179905
36-37	21.536826403089606	27.42909007402187	29.62405101898901	21.41003250389951
38-39	21.51722016192639	27.532520955196293	29.65515890459732	21.295099978279993
40-41	21.379734771777002	27.319525561554066	29.771937742772803	21.528801923896133
42-43	21.348562795201126	27.402234710899215	29.635747262495855	21.613455231403805
44-45	21.51570983472065	27.500057612713512	29.409135532756288	21.575097019809554
46-47	21.594045707815976	27.6586661492281	29.029133227794606	21.718154915161314
48-49	21.64460144385222	27.72070436795659	28.808738379015296	21.82595580917589
50-51	21.72303693094417	27.73826053190423	28.613064504420628	21.925638032730973
52-53	21.71498260031823	27.80153543074367	28.617979820955135	21.865502147982966
54-55	21.80164233370582	28.033400678863217	28.459458905130834	21.705498082300124
56-57	21.658184320466876	27.83660246170673	28.492824832723844	22.01238838510255
58-59	21.590081956323377	27.53052405518971	28.80144796763827	22.07794602084864
60-61	21.74138684767186	27.823775736445523	28.540857640696487	21.89397977518613
62-63	21.550255899775458	27.822954711668125	28.629364229893024	21.997425158663393
64-65	21.56570071805203	27.62029304431961	28.747223492585626	22.066782745042733
66-67	21.558752969969603	27.675403927388707	28.57524422310665	22.190598879535038
68-69	21.88167020106184	27.749649594792846	28.456809014989187	21.91187118915613
70-71	21.836588804075117	27.65313433399911	28.47942910752421	22.030847754401563
72-73	21.657883562949994	27.790857851991017	28.526933219029114	22.02432536602987
74-75	21.7912176496914	27.77567042011903	28.41085439244264	22.022257537746924
76-77	21.786790907932364	27.82137160864001	28.231046074492678	22.160791408934948
78-79	21.825027268983373	27.90285904315079	28.2958711774739	21.976242510391945
80-81	21.726998892309762	27.745145746016643	28.380975843641565	22.14687951803203
82-83	21.6670749545026	27.79960818194996	28.437078773126935	22.09623809042051
84-85	21.73940998896803	27.69654685752489	28.299375899789986	22.264667253717096
86-87	21.784316992517383	27.84701390122779	28.25857053407991	22.110098572174913
88-89	21.598058183066833	27.724437602480624	28.336308128045612	22.341196086406935
90-91	21.52813546635177	27.636953145543085	28.36524357122725	22.46966781687789
92-93	21.591592864983305	27.829973550539854	28.242801296731045	22.335632287745792
94-95	21.482980318229245	27.620666867550053	28.36318256900661	22.533170245214087
96-97	21.547472981095066	27.63130118473888	28.347564887776134	22.47366094638992
98-99	21.510933587266585	27.69200667957535	28.29861647244491	22.498443260713156
100	21.529087366379713	27.623889354278557	28.264460143503893	22.582563135837834
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	532.0
1	410.5
2	626.5
3	947.5
4	576.5
5	336.0
6	879.0
7	2261.0
8	3824.5
9	4373.0
10	3330.0
11	2088.5
12	1934.0
13	2206.0
14	2545.5
15	2970.0
16	3429.0
17	3959.5
18	4633.0
19	5509.5
20	6850.5
21	8498.5
22	10782.5
23	14285.0
24	19260.5
25	25505.5
26	33245.5
27	42299.5
28	52509.5
29	64905.0
30	77435.0
31	88240.0
32	97494.0
33	105864.0
34	114580.0
35	122049.5
36	128884.5
37	135692.0
38	141823.5
39	146694.5
40	149332.0
41	150914.5
42	150654.0
43	149762.0
44	148356.5
45	146125.0
46	143509.5
47	139346.5
48	134955.0
49	131545.0
50	129151.5
51	125470.0
52	118443.0
53	111869.0
54	106431.0
55	101267.0
56	95254.0
57	87262.5
58	80336.0
59	74573.5
60	66919.0
61	57287.0
62	49712.5
63	42906.0
64	35183.0
65	28636.0
66	23089.5
67	18134.0
68	14247.5
69	11277.0
70	8554.5
71	6337.5
72	4948.5
73	3875.5
74	2990.5
75	2190.5
76	1515.5
77	1059.0
78	742.0
79	512.5
80	317.0
81	153.5
82	71.5
83	39.0
84	23.0
85	16.0
86	10.0
87	4.0
88	4.5
89	3.5
90	1.5
91	2.0
92	1.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03330007934406449
2	0.0
3	0.0
4	1.1522518804174562E-4
5	0.0
6	0.0
7	0.0
8	2.995854889085386E-4
9	0.0
10-11	0.0
12-13	0.0433938058165214
14-15	1.1522518804174562E-4
16-17	0.0
18-19	2.3045037608349127E-5
20-21	1.267477068459202E-4
22-23	0.0
24-25	1.0370266923757106E-4
26-27	4.6090075216698254E-5
28-29	2.995854889085386E-4
30-31	3.4567556412523684E-5
32-33	2.534954136918404E-4
34-35	1.0370266923757106E-4
36-37	8.065763162922193E-5
38-39	2.6501793249601496E-4
40-41	2.1892785727931668E-4
42-43	4.7242327097115705E-4
44-45	2.0740533847514212E-4
46-47	1.267477068459202E-4
48-49	7.950537974880449E-4
50-51	3.226305265168877E-4
52-53	5.876484590129026E-4
54-55	3.6872060173358603E-4
56-57	5.185133461878554E-4
58-59	1.267477068459202E-4
60-61	1.3827022565009474E-4
62-63	7.489637222713466E-4
64-65	4.493782333628079E-4
66-67	0.0013596572188925983
68-69	0.0015440175197593915
70-71	4.1481067695028423E-4
72-73	7.259186846629975E-4
74-75	8.065763162922193E-4
76-77	6.913511282504738E-4
78-79	0.003456755641252369
80-81	0.0027538819941977204
82-83	0.0016131526325844387
84-85	0.0028114945882185935
86-87	0.001659242707801137
88-89	0.002166233535184818
90-91	0.00290367473865199
92-93	0.0025349541369184036
94-95	0.0037217735737483837
96-97	0.003341530453210623
98-99	0.0030188999266937354
100	0.0017053327830178352
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4339329.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.93817738435874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.72787067468815	49.69778382906706
2	13.660318409334113	17.468317231656513
3	4.001358864306069	7.675187785334329
4	1.662755954035161	4.25254340543995
5	0.859669743572768	2.7482858278260918
6	0.5018879159995229	1.925387916014618
7	0.3296098309242723	1.4752256286085235
8	0.22594775640554793	1.155735018292464
9	0.17287220300540246	0.9947820222525867
>10	0.8094820870402207	8.920911725180416
>50	0.03646248787106107	1.576230654640479
>100	0.011006868130226713	1.1250930755285933
>500	4.6874583357509725E-4	0.2059221189690705
>1k	1.8028678369418972E-4	0.17668904967904459
>5k	7.21147134775335E-5	0.3606402406218069
>10k+	3.605735673876675E-5	0.24126447088839795
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10465	0.24116631857137358	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9920	0.22860677307482333	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5723	0.13188675023258206	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.3045037608349124E-5	0.0
4	0.0	0.0	0.0	2.3045037608349124E-5	0.0
5	0.0	2.3045037608349124E-5	0.0	2.3045037608349124E-5	0.0
6	0.0	2.3045037608349124E-5	0.0	2.3045037608349124E-5	0.0
7	0.0	2.3045037608349124E-5	0.0	2.3045037608349124E-5	0.0
8	0.0	2.3045037608349124E-5	0.0	2.3045037608349124E-5	0.0
9	0.0	2.3045037608349124E-5	0.0	2.3045037608349124E-5	0.0
10-11	0.0	3.4567556412523684E-5	0.0	2.3045037608349124E-5	0.0
12-13	0.0	8.065763162922194E-5	0.0	2.3045037608349124E-5	0.0
14-15	0.0	1.1522518804174564E-4	0.0	2.3045037608349124E-5	0.0
16-17	0.0	1.3827022565009476E-4	0.0	5.761259402087282E-5	0.0
18-19	0.0	1.3827022565009476E-4	0.0	8.065763162922194E-5	0.0
20-21	0.0	1.3827022565009476E-4	0.0	1.3827022565009474E-4	0.0
22-23	1.1522518804174562E-5	1.3827022565009476E-4	0.0	2.9958548890853865E-4	0.0
24-25	2.3045037608349124E-5	1.3827022565009476E-4	0.0	5.185133461878554E-4	0.0
26-27	2.3045037608349124E-5	1.84360300866793E-4	0.0	9.678915795506633E-4	0.0
28-29	2.3045037608349124E-5	2.189278572793167E-4	0.0	0.0024658190240933564	0.0
30-31	2.3045037608349124E-5	2.534954136918404E-4	0.0	0.007270709365434148	0.0
32-33	2.3045037608349124E-5	2.534954136918404E-4	0.0	0.017813814071253875	0.0
34-35	2.3045037608349124E-5	2.534954136918404E-4	0.0	0.034544511374915335	0.0
36-37	2.3045037608349124E-5	2.534954136918404E-4	0.0	0.06173765575276731	0.0
38-39	2.3045037608349124E-5	2.9958548890853865E-4	0.0	0.09691590566191224	0.0
40-41	4.609007521669825E-5	3.4567556412523685E-4	0.0	0.13423734406863363	0.0
42-43	4.609007521669825E-5	3.4567556412523685E-4	0.0	0.17069459356504196	0.0
44-45	4.609007521669825E-5	3.4567556412523685E-4	0.0	0.21573611957056033	0.0
46-47	4.609007521669825E-5	3.68720601733586E-4	0.0	0.26857839080650486	0.0
48-49	4.609007521669825E-5	3.68720601733586E-4	0.0	0.32216962576472075	0.0
50-51	4.609007521669825E-5	3.68720601733586E-4	0.0	0.3791715262889723	0.0
52-53	4.609007521669825E-5	3.8024312053776054E-4	0.0	0.4338343554959765	0.0
54-55	4.609007521669825E-5	3.917656393419351E-4	0.0	0.495191768128206	0.0
56-57	4.609007521669825E-5	4.0328815814610967E-4	0.0	0.5678873392637434	0.0
58-59	4.609007521669825E-5	4.609007521669825E-4	0.0	0.6454454133346423	0.0
60-61	5.761259402087282E-5	4.609007521669825E-4	0.0	0.7245359824064965	0.0
62-63	6.913511282504738E-5	4.954683085795062E-4	0.0	0.8003195885815526	0.0
64-65	6.913511282504738E-5	5.300358649920299E-4	0.0	0.8826249403997715	0.0
66-67	6.913511282504738E-5	5.300358649920299E-4	0.0	0.9752199015101183	0.0
68-69	6.913511282504738E-5	5.876484590129026E-4	0.0	1.0754427700688285	0.0
70-71	6.913511282504738E-5	6.106934966212518E-4	0.0	1.1797676553218253	0.0
72-73	6.913511282504738E-5	6.913511282504737E-4	0.0	1.2879064942990035	0.0
74-75	6.913511282504738E-5	7.37441203467172E-4	0.0	1.398107864142129	0.0
76-77	6.913511282504738E-5	8.526663915089176E-4	0.0	1.5178844471115234	0.0
78-79	9.21801504333965E-5	8.526663915089176E-4	0.0	1.645496342867757	0.0
80-81	9.21801504333965E-5	8.526663915089176E-4	0.0	1.7872578917155164	0.0
82-83	9.21801504333965E-5	8.526663915089176E-4	0.0	1.9321420431592071	0.0
84-85	9.21801504333965E-5	8.526663915089176E-4	0.0	2.0772220774225696	0.0
86-87	9.21801504333965E-5	9.102789855297904E-4	0.0	2.233156324399464	0.0
88	9.21801504333965E-5	9.21801504333965E-4	0.0	2.356032464927181	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6805	0.0	57.208237	1
GTATCAA	12210	0.0	40.547962	1
TCAACGC	15185	0.0	31.846134	4
ATCAACG	15290	0.0	31.68891	3
CAACGCA	15770	0.0	30.694578	5
TATCAAC	16175	0.0	30.01319	2
AACGCAG	16325	0.0	29.62181	6
GTGGTAT	2835	0.0	29.354626	1
ACGCAGA	18900	0.0	25.337437	7
GTACATG	11010	0.0	25.323498	1
CGCAGAG	19360	0.0	24.61392	8
TGGTATC	3235	0.0	24.26042	2
TACATGG	11040	0.0	24.221405	2
ACATGGG	11400	0.0	22.467138	3
GCAGAGT	20990	0.0	22.254276	9
AGAGTAC	19195	0.0	20.843128	10-11
GAGTACT	15185	0.0	20.730255	12-13
CAGAGTA	20330	0.0	20.362072	10-11
CATGGGG	8785	0.0	19.311745	4
AGTACTT	16035	0.0	19.059576	12-13
>>END_MODULE