FastQCFastQC Report
Wed 25 May 2016
SRR2049340_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049340_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5636377
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51150.048.160541
GTATCAA91300.037.897691
TCAACGC98550.034.0097854
ATCAACG99950.033.580443
CAACGCA103400.032.3693775
AACGCAG108300.030.9012686
TATCAAC113150.029.9537852
ACGCAGA125250.026.4942557
CGCAGAG127550.025.9428088
GTACATG137250.023.7027871
TACATGG140800.022.3688242
GCAGAGT147850.021.522479
ACATGGG143200.021.271743
GTGGTAT32700.019.1209551
CATGGGG105300.018.6603134
AGAGTAC128750.018.56386810-11
CAGAGTA141300.018.37870410-11
GAGTACA110000.017.0524161
GAGTACT69450.016.95338212-13
TGGTATC33500.015.9967232
Produced by FastQC (version 0.11.5)