##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049340_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5636377
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.839997750327914	31.0	31.0	34.0	28.0	34.0
2	31.039622615733474	31.0	31.0	34.0	28.0	34.0
3	31.091377315605396	31.0	31.0	34.0	28.0	34.0
4	34.692760792970375	37.0	35.0	37.0	32.0	37.0
5	34.71555965826984	37.0	35.0	37.0	32.0	37.0
6	34.69477094949468	37.0	35.0	37.0	32.0	37.0
7	34.647587803299885	37.0	35.0	37.0	32.0	37.0
8	34.61439272071404	37.0	35.0	37.0	32.0	37.0
9	36.20058257281229	39.0	37.0	39.0	32.0	39.0
10-11	36.15917281615478	39.0	37.0	39.0	32.0	39.0
12-13	36.10620031271861	39.0	37.0	39.0	32.0	39.0
14-15	37.32280958140309	40.0	38.0	41.0	32.0	41.0
16-17	37.29001644141263	40.0	38.0	41.0	32.0	41.0
18-19	37.318181164957565	40.0	38.0	41.0	32.0	41.0
20-21	37.39849649872605	40.0	38.0	41.0	32.0	41.0
22-23	37.34377428976096	40.0	38.0	41.0	32.0	41.0
24-25	37.34650095265097	40.0	38.0	41.0	32.0	41.0
26-27	37.32879516398566	40.0	38.0	41.0	32.0	41.0
28-29	37.2569016408945	40.0	38.0	41.0	32.0	41.0
30-31	37.17817456142483	40.0	37.0	41.0	31.0	41.0
32-33	37.107438164622415	40.0	37.0	41.0	31.0	41.0
34-35	36.98716622397686	40.0	37.0	41.0	31.0	41.0
36-37	36.891588071557315	40.0	37.0	41.0	30.0	41.0
38-39	36.77494221908861	40.0	37.0	41.0	30.0	41.0
40-41	36.66354370901733	40.0	37.0	41.0	30.0	41.0
42-43	36.5156735434837	40.0	37.0	41.0	30.0	41.0
44-45	36.38864877207469	40.0	36.0	41.0	30.0	41.0
46-47	36.2717850491548	40.0	36.0	41.0	30.0	41.0
48-49	36.13896763825415	40.0	36.0	41.0	29.0	41.0
50-51	35.99442319064179	39.5	35.5	41.0	29.0	41.0
52-53	35.85098415169887	39.0	35.0	41.0	28.5	41.0
54-55	35.708802480742506	39.0	35.0	41.0	28.0	41.0
56-57	35.57811933091062	39.0	35.0	41.0	28.0	41.0
58-59	35.37057049235706	39.0	35.0	41.0	28.0	41.0
60-61	35.14750636800909	39.0	35.0	40.0	27.0	41.0
62-63	34.931700895805946	38.0	34.5	40.0	27.0	41.0
64-65	34.67197891482419	38.0	34.0	40.0	26.0	41.0
66-67	34.385819028783914	37.0	34.0	40.0	26.0	41.0
68-69	34.05698722778835	37.0	34.0	40.0	26.0	41.0
70-71	33.72372802599968	36.0	34.0	39.0	25.5	41.0
72-73	33.31360721612482	36.0	33.0	39.0	24.5	41.0
74-75	32.89265205290562	35.0	33.0	39.0	23.0	40.0
76-77	32.47252685546052	35.0	33.0	37.5	21.5	40.0
78-79	32.04920297205102	35.0	32.5	37.0	20.0	39.0
80-81	31.56065057394138	35.0	32.0	37.0	15.5	39.0
82-83	31.112443064046282	35.0	32.0	36.0	8.5	38.5
84-85	30.694622095008903	35.0	32.0	36.0	2.0	37.0
86-87	30.244082945480756	35.0	31.0	35.5	2.0	37.0
88-89	29.850751111928815	35.0	31.0	35.0	2.0	36.5
90-91	29.490606110982284	35.0	31.0	35.0	2.0	36.0
92-93	29.103553843186855	34.5	30.5	35.0	2.0	36.0
94-95	28.766936810649817	34.0	30.0	35.0	2.0	36.0
96-97	28.38201170716579	34.0	30.0	35.0	2.0	35.0
98-99	28.05729398512555	34.0	29.0	35.0	2.0	35.0
100	27.852570543098874	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3270646650586926
1101	2	0.3161619118768364
1101	3	0.31823914912397555
1101	4	0.3815373482687576
1101	5	0.3737850709522661
1101	6	0.42400795231277755
1101	7	0.38778687453755367
1101	8	0.4062465616401312
1101	9	0.5830788716895015
1101	10-11	0.4599886430187681
1101	12-13	0.49450130449434937
1101	14-15	0.5687969948001239
1101	16-17	0.5955527886052607
1101	18-19	0.528580227221056
1101	20-21	0.5525553597010244
1101	22-23	0.596321716549916
1101	24-25	0.5937230317950295
1101	26-27	0.560996646077335
1101	28-29	0.5640363743557941
1101	30-31	0.5791173054120904
1101	32-33	0.5797353385918314
1101	34-35	0.6744276753922804
1101	36-37	0.6020103962294101
1101	38-39	0.642252551075309
1101	40-41	0.648602103440993
1101	42-43	0.6292525149523129
1101	44-45	0.5927868821088254
1101	46-47	0.5490021713745961
1101	48-49	0.4600553997707948
1101	50-51	0.556032247108341
1101	52-53	0.4927737183298859
1101	54-55	0.5188089326990308
1101	56-57	0.4531584577744283
1101	58-59	0.5483257879464105
1101	60-61	0.5476501568113292
1101	62-63	0.5491483800007444
1101	64-65	0.5336400644083099
1101	66-67	0.5380120604356549
1101	68-69	0.48620738602994606
1101	70-71	0.500154729406546
1101	72-73	0.616091865171903
1101	74-75	0.507734573532872
1101	76-77	0.525863221345638
1101	78-79	0.4968969711506972
1101	80-81	0.48285983268688426
1101	82-83	0.5522147119120362
1101	84-85	0.44905693750332176
1101	86-87	0.5407647167298855
1101	88-89	0.5440614709298899
1101	90-91	0.49004686124684227
1101	92-93	0.5658111573625852
1101	94-95	0.5674962840006863
1101	96-97	0.5517565396423301
1101	98-99	0.5894791618431938
1101	100	0.5469983102273162
1102	1	0.18412325464342416
1102	2	0.2790990822988739
1102	3	0.26634039992197955
1102	4	0.3101009746859944
1102	5	0.2426959591256761
1102	6	0.35075433145321
1102	7	0.38810224469332155
1102	8	0.39214730703454137
1102	9	0.4715048470719978
1102	10-11	0.4513974731727828
1102	12-13	0.4906499272252489
1102	14-15	0.5985262287120747
1102	16-17	0.540775666959675
1102	18-19	0.5346117002310677
1102	20-21	0.5351695844726478
1102	22-23	0.4956375030423672
1102	24-25	0.5491193396954586
1102	26-27	0.5023357687593304
1102	28-29	0.49690039362598526
1102	30-31	0.5254384201426774
1102	32-33	0.6022380284032707
1102	34-35	0.5568344980852871
1102	36-37	0.5063973468464127
1102	38-39	0.5123651212429152
1102	40-41	0.5307982787026617
1102	42-43	0.5056441665228277
1102	44-45	0.4232660328961799
1102	46-47	0.4198144455990942
1102	48-49	0.45165857546863464
1102	50-51	0.5177941791509539
1102	52-53	0.4914978139169506
1102	54-55	0.5029097844388488
1102	56-57	0.4681905259160217
1102	58-59	0.5506109820312801
1102	60-61	0.4553951460092662
1102	62-63	0.49363519432796465
1102	64-65	0.5339574629095267
1102	66-67	0.5824181015395453
1102	68-69	0.5632984862360715
1102	70-71	0.5805419660953746
1102	72-73	0.519197727826473
1102	74-75	0.45303753397066515
1102	76-77	0.4848201018765437
1102	78-79	0.4241888249697965
1102	80-81	0.4384502607594314
1102	82-83	0.4332674262063563
1102	84-85	0.3960540922605169
1102	86-87	0.46015751724541687
1102	88-89	0.44030912199317385
1102	90-91	0.4645446992559634
1102	92-93	0.3835371988090479
1102	94-95	0.43350393371774487
1102	96-97	0.38330898566918137
1102	98-99	0.41169468301303525
1102	100	0.4056660538702914
1103	1	0.08215908888008272
1103	2	0.14168512581780845
1103	3	0.15290218229315755
1103	4	0.11896582994852167
1103	5	0.09805637575457382
1103	6	0.16430049705100203
1103	7	0.22386560632390484
1103	8	0.17289993669560033
1103	9	0.2888152352751092
1103	10-11	0.26259984223455035
1103	12-13	0.2715908471502786
1103	14-15	0.2906647169206309
1103	16-17	0.34996583369967027
1103	18-19	0.3045217889922327
1103	20-21	0.24505932196263558
1103	22-23	0.2235202073202771
1103	24-25	0.28346482192332445
1103	26-27	0.2529719558076309
1103	28-29	0.20442108373330115
1103	30-31	0.26544409666728797
1103	32-33	0.2980301198860573
1103	34-35	0.32963505342166854
1103	36-37	0.23432997729977245
1103	38-39	0.24718849912421348
1103	40-41	0.2669897105268646
1103	42-43	0.27380434523264086
1103	44-45	0.1597296606941896
1103	46-47	0.1984964338944053
1103	48-49	0.23768872800101093
1103	50-51	0.18775088965577424
1103	52-53	0.1937253222708364
1103	54-55	0.23158032472447587
1103	56-57	0.08962182351793757
1103	58-59	0.1657604540943467
1103	60-61	0.11281578233443668
1103	62-63	0.15037842213720864
1103	64-65	0.1749971819877416
1103	66-67	0.17159605866788752
1103	68-69	0.21607279891665598
1103	70-71	0.18111448168303923
1103	72-73	0.23283650863790228
1103	74-75	0.2060011152956136
1103	76-77	0.27477660519492986
1103	78-79	0.23262952356024158
1103	80-81	0.231356029395684
1103	82-83	0.2647993406387421
1103	84-85	0.376307306695697
1103	86-87	0.3407390934027852
1103	88-89	0.33012598549174754
1103	90-91	0.4039231955869873
1103	92-93	0.3130250368214824
1103	94-95	0.3702645191460334
1103	96-97	0.37352702828877327
1103	98-99	0.41139500606081114
1103	100	0.3834050225595327
1104	1	0.2128494992173735
1104	2	0.28362229090859614
1104	3	0.2721043935061651
1104	4	0.2889712931556332
1104	5	0.3268058556745288
1104	6	0.32313958598737713
1104	7	0.4447520059977208
1104	8	0.4195201623153011
1104	9	0.42015794375659254
1104	10-11	0.45590869337222983
1104	12-13	0.37509182839195887
1104	14-15	0.5023736177981704
1104	16-17	0.4948831805409242
1104	18-19	0.5151963458809945
1104	20-21	0.5061318735651028
1104	22-23	0.5482753134196372
1104	24-25	0.550387601982365
1104	26-27	0.5359105508440294
1104	28-29	0.48977833310015484
1104	30-31	0.47290315404217154
1104	32-33	0.49073888157072076
1104	34-35	0.5132541562591584
1104	36-37	0.487355127893899
1104	38-39	0.5981518153621082
1104	40-41	0.5993608257163174
1104	42-43	0.5944329351481343
1104	44-45	0.6099592305033639
1104	46-47	0.4893863656444495
1104	48-49	0.5485171240764117
1104	50-51	0.5696717787211725
1104	52-53	0.5577404099312986
1104	54-55	0.5686799766816861
1104	56-57	0.5730754700913039
1104	58-59	0.5885760005006375
1104	60-61	0.5495160234153005
1104	62-63	0.5605529903098443
1104	64-65	0.649741413169707
1104	66-67	0.6781258634388792
1104	68-69	0.614439418130516
1104	70-71	0.5548550700705945
1104	72-73	0.6200384499469749
1104	74-75	0.605682554431894
1104	76-77	0.5963555159735066
1104	78-79	0.6198455431920493
1104	80-81	0.5951493929394758
1104	82-83	0.5246991126744192
1104	84-85	0.5403955122722301
1104	86-87	0.6088367204746028
1104	88-89	0.5038198783774455
1104	90-91	0.44935873678603855
1104	92-93	0.47291906847597787
1104	94-95	0.5118848803235458
1104	96-97	0.4943343424734685
1104	98-99	0.4300952974268064
1104	100	0.46241784057324153
1105	1	0.2447923557631313
1105	2	0.2840500597044624
1105	3	0.24596185411176563
1105	4	0.3117544494710387
1105	5	0.31374514327661984
1105	6	0.3058950122770696
1105	7	0.36214665189761774
1105	8	0.3932680864367839
1105	9	0.5035180186449537
1105	10-11	0.4489826366244003
1105	12-13	0.44552198609206783
1105	14-15	0.4249431779695527
1105	16-17	0.4634486957893742
1105	18-19	0.4377865522383644
1105	20-21	0.4355088379704384
1105	22-23	0.43549327048494035
1105	24-25	0.46918096392730035
1105	26-27	0.4274254368161863
1105	28-29	0.5022135345229799
1105	30-31	0.47627021188139906
1105	32-33	0.47356997451062455
1105	34-35	0.4968456990336989
1105	36-37	0.4922226554068345
1105	38-39	0.49022605803802577
1105	40-41	0.4855477706248763
1105	42-43	0.5042837601488372
1105	44-45	0.502934699015043
1105	46-47	0.4709494296537571
1105	48-49	0.5010186815482953
1105	50-51	0.5155384879533287
1105	52-53	0.5637417481500506
1105	54-55	0.6075758777471805
1105	56-57	0.5837282174572991
1105	58-59	0.5760759419490569
1105	60-61	0.5350836187759995
1105	62-63	0.4869706680571113
1105	64-65	0.5435164980154426
1105	66-67	0.5384516407966089
1105	68-69	0.5136435851289747
1105	70-71	0.5116408372457499
1105	72-73	0.45880413231062533
1105	74-75	0.5251800846363963
1105	76-77	0.5853481802719287
1105	78-79	0.5165683845396174
1105	80-81	0.5825292406044937
1105	82-83	0.5305147433145159
1105	84-85	0.5876874569087427
1105	86-87	0.540819824471928
1105	88-89	0.5384324345013809
1105	90-91	0.5943296609749034
1105	92-93	0.59884321950517
1105	94-95	0.5495206434091067
1105	96-97	0.5110961989871612
1105	98-99	0.48063485469042533
1105	100	0.5075918396385788
1106	1	0.15455961129500295
1106	2	0.11169452477949449
1106	3	0.1369585351868281
1106	4	0.1590861464405009
1106	5	0.16718390038808906
1106	6	0.23003431611526537
1106	7	0.25244282572481325
1106	8	0.2508760321864685
1106	9	0.3548404736839075
1106	10-11	0.23881992764361115
1106	12-13	0.26899602275435086
1106	14-15	0.32119524948390676
1106	16-17	0.37636485399264075
1106	18-19	0.3769270934269642
1106	20-21	0.3641037529970461
1106	22-23	0.33379194657887723
1106	24-25	0.3346985743013491
1106	26-27	0.31154992985056396
1106	28-29	0.39005230205133046
1106	30-31	0.3882896875620432
1106	32-33	0.43070103648003055
1106	34-35	0.4632211918354159
1106	36-37	0.4262067318534051
1106	38-39	0.4577802231991299
1106	40-41	0.462921989090745
1106	42-43	0.47750657623922166
1106	44-45	0.4435046517699419
1106	46-47	0.4823925694721325
1106	48-49	0.5230403881305037
1106	50-51	0.49176921872285106
1106	52-53	0.5241457941369632
1106	54-55	0.5053363947234644
1106	56-57	0.4585430356582165
1106	58-59	0.5098861729149036
1106	60-61	0.5196315536890808
1106	62-63	0.47241743258839364
1106	64-65	0.4970569581962039
1106	66-67	0.49719734634572177
1106	68-69	0.46879955465379197
1106	70-71	0.46554469681628063
1106	72-73	0.5249507308714243
1106	74-75	0.5111141775290307
1106	76-77	0.5776683864706698
1106	78-79	0.45908886281000605
1106	80-81	0.4934990843700433
1106	82-83	0.47958330208491873
1106	84-85	0.4397514772039166
1106	86-87	0.37063763510073144
1106	88-89	0.4090015374219931
1106	90-91	0.4336887141583894
1106	92-93	0.4171842848067264
1106	94-95	0.4575089332883415
1106	96-97	0.4034708790785757
1106	98-99	0.3825796246045492
1106	100	0.42679833683291335
1107	1	0.23220362702604547
1107	2	0.2054114492952479
1107	3	0.1879833870858718
1107	4	0.24928289495874623
1107	5	0.21456889589661898
1107	6	0.24249794379234402
1107	7	0.23180377059485835
1107	8	0.32437477874098875
1107	9	0.3270567880446933
1107	10-11	0.25767704856718154
1107	12-13	0.25506944506940954
1107	14-15	0.3240377360960025
1107	16-17	0.3680238227438579
1107	18-19	0.3294602569579439
1107	20-21	0.29962424450460645
1107	22-23	0.3553885590902013
1107	24-25	0.2935327391681355
1107	26-27	0.3233071475358358
1107	28-29	0.4082255916535331
1107	30-31	0.34458228707890726
1107	32-33	0.38884938767195365
1107	34-35	0.383982127434777
1107	36-37	0.3795032829198348
1107	38-39	0.46257112811286305
1107	40-41	0.44172391699201796
1107	42-43	0.44292948609306393
1107	44-45	0.4445255436658613
1107	46-47	0.4295066639423908
1107	48-49	0.42818347595211037
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2209	2	0.27342846763140116
2209	3	0.27674738628877904
2209	4	0.24247498629181052
2209	5	0.2878726788153969
2209	6	0.25782186980998034
2209	7	0.32767275931670525
2209	8	0.27299609055784657
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2210	4	-0.37759393824873655
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2210	32-33	-0.4751246759743708
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2214	96-97	-0.12221432107847718
2214	98-99	-0.07648959989584725
2214	100	-0.05919367269369147
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2215	4	-0.31753886745147497
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2215	26-27	-0.5539799597280748
2215	28-29	-0.5643787917631684
2215	30-31	-0.5571124074140883
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2215	84-85	-0.31573911958213685
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2215	90-91	-0.19221595943309566
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2215	100	-0.2916097386388863
2216	1	-1.2006097569617928
2216	2	-1.2299673463529004
2216	3	-1.2494667957413164
2216	4	-1.4434412878795513
2216	5	-1.4117999368745302
2216	6	-2.7986668340619296
2216	7	-2.749739083411942
2216	8	-3.6286000643563874
2216	9	-3.1472725183554147
2216	10-11	-2.2772639333073386
2216	12-13	-2.0120231383030784
2216	14-15	-2.23475494821691
2216	16-17	-2.697544914351937
2216	18-19	-2.3274305859821567
2216	20-21	-2.1692726031813123
2216	22-23	-2.2696320812192923
2216	24-25	-2.490545426509769
2216	26-27	-2.4948726284297393
2216	28-29	-2.8957896771214493
2216	30-31	-2.508994449086323
2216	32-33	-2.686732119287015
2216	34-35	-3.7272622731224914
2216	36-37	-2.727789311481608
2216	38-39	-2.583096159918391
2216	40-41	-3.1306481454936943
2216	42-43	-2.951356085573096
2216	44-45	-2.6636881562094032
2216	46-47	-2.534769638154927
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2216	50-51	-3.0196871103864567
2216	52-53	-3.3798321174162993
2216	54-55	-2.7553847672808374
2216	56-57	-2.7392822317261576
2216	58-59	-3.7236420149398626
2216	60-61	-4.451396398788127
2216	62-63	-3.559230481335149
2216	64-65	-3.5380267424258918
2216	66-67	-3.15793843230918
2216	68-69	-3.5588823336028383
2216	70-71	-3.32144688629997
2216	72-73	-3.747485757188759
2216	74-75	-4.130692301592422
2216	76-77	-4.626218392697577
2216	78-79	-3.6825879455000745
2216	80-81	-3.48832784529689
2216	82-83	-3.655172861822905
2216	84-85	-3.694796048762985
2216	86-87	-3.23814483761425
2216	88-89	-3.1547568821556737
2216	90-91	-3.277127598429683
2216	92-93	-3.298510248059255
2216	94-95	-3.199236047800003
2216	96-97	-3.4208243914452474
2216	98-99	-3.124251857509247
2216	100	-2.707601758428332
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107219.0
3	13797.0
4	8879.0
5	7595.0
6	7311.0
7	8122.0
8	9733.0
9	11308.0
10	12687.0
11	14131.0
12	15838.0
13	17539.0
14	19485.0
15	19766.0
16	19383.0
17	19927.0
18	19348.0
19	18200.0
20	18486.0
21	19503.0
22	22211.0
23	26082.0
24	31493.0
25	39640.0
26	49033.0
27	60754.0
28	75573.0
29	95750.0
30	117042.0
31	136992.0
32	167991.0
33	213479.0
34	281354.0
35	393936.0
36	626084.0
37	1061813.0
38	1359068.0
39	488595.0
40	1230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.12056120289704	20.41973987626422	13.904047542363934	23.555651378474803
2	15.768081885191055	20.351299112744776	40.17991810770948	23.700700894354686
3	18.344031774779317	25.24631027124924	30.33632023547812	26.073337718493317
4	12.933298899630193	16.812060315778357	38.71033357206184	31.54430721252961
5	13.187763872603469	37.43335402957184	35.71586798000807	13.663014117816621
6	30.95650346495174	37.9236807171615	17.63407773781596	13.4857380800708
7	26.818852169356926	32.38023041524548	22.74933889045771	18.051578524939877
8	24.692285792786954	36.19586647365234	20.845316858485898	18.266530875074814
9	24.656806052822333	16.929280322150777	21.860776424833496	36.55313720019339
10-11	23.72385119184963	26.927557196357892	29.160753270264994	20.187838341527485
12-13	24.137981528010783	24.559304116190788	29.007973668647246	22.294740687151183
14-15	21.650126882662267	26.868702735154464	26.16999836266122	25.31117201952205
16-17	21.216071567072035	30.09171643159258	26.82241504436292	21.869796956972472
18-19	21.05398796591437	29.080149083368728	28.827511735105993	21.03835121561091
20-21	22.216245823053107	28.4101697508377	28.295077969379502	21.078506456729695
22-23	21.91073696317181	28.311799415526963	28.23922394475816	21.53823967654307
24-25	21.768683758153482	28.391961025325923	28.22601392127594	21.613341295244656
26-27	21.48531802051782	28.265574810953296	28.393105683345755	21.856001485183132
28-29	21.544029100409368	28.36746809932405	28.438923483636348	21.649579316630238
30-31	21.781107948230115	28.7015047798037	28.02781615644023	21.48957111552595
32-33	21.45296919917427	28.42575568877537	28.409444869519657	21.7118302425307
34-35	21.632126295003257	28.01715137486898	28.516592254885943	21.834130075241813
36-37	21.861286641021437	28.323456438888893	28.03452832500939	21.78072859508028
38-39	21.65348992318999	28.296797790438845	28.31942655342465	21.73028573294652
40-41	21.45341708498566	27.93717436942444	28.650698620152987	21.958709925436914
42-43	21.2473968896764	27.917411463633307	28.71071285666505	22.124478790025247
44-45	21.31442565490869	28.000762455052026	28.68937685893898	21.995435031100303
46-47	21.24128658087532	28.238217710954743	28.488994867232307	22.031500840937625
48-49	21.08517834108678	28.047333171946182	28.56075196947937	22.30673651748767
50-51	21.08750474540071	27.931149049863848	28.519392382830976	22.46195382190447
52-53	20.988371917605086	27.882319553696888	28.61175246171848	22.517556066979544
54-55	21.09171930513933	28.14682315824802	28.397567488924558	22.363890047688102
56-57	21.07386533449853	27.918143573441164	28.36141083079955	22.646580261260755
58-59	21.03500090558675	27.472582374007416	28.5185727810884	22.973843939317433
60-61	21.243830086416725	27.75612989228236	28.13887906104171	22.861160960259202
62-63	21.183273440284193	27.688966592882974	28.07831873987486	23.049441226957978
64-65	21.211938629728554	27.39128428562555	28.212737259634586	23.18403982501131
66-67	21.122837867049256	27.540136847384677	27.929720984531635	23.407304301034433
68-69	21.408015186328825	27.605200884579517	27.851316739791514	23.13546718930014
70-71	21.273202303964425	27.365194760387062	27.992042541062485	23.36956039458603
72-73	21.094670366159647	27.299629825902105	28.200431757381033	23.40526805055721
74-75	21.174973436545244	27.163885556810296	28.186403309113427	23.47473769753103
76-77	21.252042744653725	27.15900007382987	28.09779298389089	23.49116419762552
78-79	21.38861138506541	27.201126903144296	28.25655149109327	23.15371022069703
80-81	21.32850644940246	27.032312944789055	28.338521263354878	23.30065934245361
82-83	21.204446004637038	26.9999353995079	28.42107523614674	23.37454335970832
84-85	21.326405962349835	26.76772104710273	28.399047165553565	23.506825824993875
86-87	21.625947491622444	26.786458577825712	28.315889019324263	23.27170491122758
88-89	21.692472678401796	26.57494909714824	28.364759475428862	23.3678187490211
90-91	21.904958905767675	26.422733277143678	28.28075447480686	23.391553342281792
92-93	22.14015362099208	26.41181070856815	28.00607285563267	23.4419628148071
94-95	22.112201702918156	26.070091877644252	28.143424146701808	23.674282272735784
96-97	22.232002933799496	25.95917554840636	28.161778390622207	23.64704312717194
98-99	22.369688897673097	25.986311774389826	28.03069241110025	23.613306916836827
100	22.43758712378537	25.983730328897447	28.046314148255163	23.53236839906202
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1405.0
1	1115.5
2	1006.5
3	1237.0
4	1194.5
5	1029.0
6	894.5
7	824.0
8	839.0
9	826.0
10	748.5
11	691.0
12	728.0
13	810.0
14	914.5
15	1069.5
16	1298.5
17	1696.0
18	2274.5
19	3066.5
20	4329.5
21	6308.0
22	8991.0
23	12983.5
24	18747.0
25	26101.0
26	35724.0
27	47415.0
28	61566.0
29	78414.5
30	94551.0
31	108540.5
32	120616.5
33	131586.0
34	144382.5
35	156068.0
36	166167.5
37	177703.0
38	187795.0
39	194028.5
40	198765.0
41	203582.5
42	204826.0
43	205014.0
44	204173.5
45	201311.0
46	198312.0
47	192038.5
48	185788.0
49	181170.5
50	177111.0
51	172000.5
52	163805.5
53	156564.5
54	149328.0
55	140199.0
56	128376.5
57	114971.0
58	103140.5
59	92157.5
60	80110.5
61	67523.0
62	57391.0
63	48693.0
64	40383.0
65	33485.5
66	27686.5
67	22239.5
68	17507.5
69	13948.5
70	11148.5
71	8820.5
72	6766.5
73	5158.5
74	4037.5
75	3014.5
76	2287.5
77	1843.0
78	1555.5
79	1465.0
80	1082.0
81	561.0
82	393.5
83	301.5
84	219.5
85	160.0
86	127.5
87	101.5
88	75.5
89	59.5
90	48.0
91	41.0
92	39.0
93	34.0
94	28.0
95	31.0
96	38.5
97	36.5
98	38.5
99	61.0
100	127.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01742253933688254
2	0.038091845169334844
3	0.0394402290691343
4	0.04190635225429385
5	0.04440795922629022
6	0.04236764148317261
7	0.04059345214133121
8	0.03997248587168672
9	0.03983055072433941
10-11	0.04039829131372866
12-13	0.04139183734515984
14-15	0.039644260843446064
16-17	0.03644184908142234
18-19	0.03928055202836858
20-21	0.03864184386530567
22-23	0.03977732504408417
24-25	0.03639749434787631
26-27	0.03645959097484075
28-29	0.03711604103132207
30-31	0.03721362144512335
32-33	0.036903138310301106
34-35	0.036841041683336656
36-37	0.03425072524424821
38-39	0.03468540163299935
40-41	0.035847495651905474
42-43	0.035687818611139746
44-45	0.034534595538942836
46-47	0.03357653329434848
48-49	0.03358540424105769
50-51	0.03502249760794922
52-53	0.035483786836827985
54-55	0.03464104689945332
56-57	0.032875728504321126
58-59	0.0338604035890431
60-61	0.03331040489307227
62-63	0.03351443666738403
64-65	0.030640249933600965
66-67	0.03156282839135849
68-69	0.03256524536949888
70-71	0.03397572589626279
72-73	0.030711217507274617
74-75	0.03115476484273497
76-77	0.0319531500465636
78-79	0.032290246021513466
80-81	0.03236121359518712
82-83	0.030959604015132414
84-85	0.032290246021513466
86-87	0.03244105211556998
88-89	0.03316846974572496
90-91	0.033496694773965614
92-93	0.03185556963276232
94-95	0.033221695425980205
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5636377.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.1266060363953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.2283813977187	50.94748976154101
2	13.23424226258525	17.23802564051205
3	3.9970103860745123	7.809351622117652
4	1.6761343431327385	4.366437641171124
5	0.8498189119445044	2.7672910740243926
6	0.5118932277045899	2.000272114404937
7	0.31311827096366396	1.4274631193093472
8	0.20869019307287887	1.0873027190333322
9	0.15572711376688586	0.9127780548732799
>10	0.7794182478089995	8.75371679208811
>50	0.03604561177351305	1.5939182454358072
>100	0.009329205146206394	0.9724379854071109
>500	1.0904667997348629E-4	0.038740519228790814
>1k	8.17816276524596E-5	0.08477471085291667
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.7741893418413992E-5	0.0
4	0.0	0.0	0.0	3.5483786836827985E-5	0.0
5	0.0	0.0	0.0	7.096757367365597E-5	0.0
6	0.0	0.0	0.0	7.096757367365597E-5	0.0
7	0.0	0.0	0.0	7.096757367365597E-5	0.0
8	0.0	0.0	0.0	7.096757367365597E-5	0.0
9	0.0	0.0	0.0	1.2419325392889794E-4	0.0
10-11	0.0	0.0	8.870946709206996E-6	1.7741893418413992E-4	0.0
12-13	0.0	0.0	1.7741893418413992E-5	2.129027210209679E-4	0.0
14-15	0.0	0.0	1.7741893418413992E-5	2.5725745456700285E-4	0.0
16-17	0.0	0.0	1.7741893418413992E-5	2.9274124140383085E-4	0.0
18-19	0.0	1.7741893418413992E-5	1.7741893418413992E-5	3.814507084959008E-4	0.0
20-21	0.0	1.7741893418413992E-5	1.7741893418413992E-5	6.919338433181456E-4	0.0
22-23	0.0	1.7741893418413992E-5	1.7741893418413992E-5	0.0016588670346217083	0.0
24-25	0.0	1.7741893418413992E-5	1.7741893418413992E-5	0.004630634182206052	0.0
26-27	0.0	1.7741893418413992E-5	1.7741893418413992E-5	0.010645136051048394	0.0
28-29	0.0	1.7741893418413992E-5	1.7741893418413992E-5	0.037284589018797006	0.0
30-31	0.0	1.7741893418413992E-5	1.7741893418413992E-5	0.1170343999345679	0.0
32-33	0.0	1.7741893418413992E-5	1.7741893418413992E-5	0.2926259190966112	0.0
34-35	0.0	3.5483786836827985E-5	1.7741893418413992E-5	0.5643341458529123	0.0
36-37	0.0	3.5483786836827985E-5	1.7741893418413992E-5	0.9746420439938634	0.0
38-39	0.0	3.5483786836827985E-5	1.7741893418413992E-5	1.4839319655161463	0.0
40-41	0.0	3.5483786836827985E-5	1.7741893418413992E-5	1.9911549564551838	0.0
42-43	0.0	3.5483786836827985E-5	1.7741893418413992E-5	2.47993524918578	0.0
44-45	0.0	5.3225680255241974E-5	1.7741893418413992E-5	3.0524217950644537	0.0
46-47	0.0	5.3225680255241974E-5	1.7741893418413992E-5	3.6938001130868283	0.0
48-49	0.0	1.0645136051048395E-4	1.7741893418413992E-5	4.33062763544738	0.0
50-51	0.0	1.0645136051048395E-4	1.7741893418413992E-5	4.939618127034441	0.0
52-53	0.0	1.0645136051048395E-4	1.7741893418413992E-5	5.500776473965457	0.0
54-55	8.870946709206996E-6	1.0645136051048395E-4	1.7741893418413992E-5	6.096256513714395	0.0
56-57	1.7741893418413992E-5	1.2419325392889794E-4	1.7741893418413992E-5	6.764558154999213	0.0
58-59	1.7741893418413992E-5	1.2419325392889794E-4	3.5483786836827985E-5	7.444471865526383	0.0
60-61	1.7741893418413992E-5	1.2419325392889794E-4	3.5483786836827985E-5	8.107388842158713	8.870946709206996E-6
62-63	1.7741893418413992E-5	1.2419325392889794E-4	3.5483786836827985E-5	8.724540604718243	1.7741893418413992E-5
64-65	1.7741893418413992E-5	1.2419325392889794E-4	3.5483786836827985E-5	9.360241516846726	1.7741893418413992E-5
66-67	1.7741893418413992E-5	1.2419325392889794E-4	3.5483786836827985E-5	10.049230205857416	1.7741893418413992E-5
68-69	1.7741893418413992E-5	1.2419325392889794E-4	3.5483786836827985E-5	10.765044637716745	1.7741893418413992E-5
70-71	1.7741893418413992E-5	1.2419325392889794E-4	3.5483786836827985E-5	11.483821965776952	1.7741893418413992E-5
72-73	1.7741893418413992E-5	1.2419325392889794E-4	3.5483786836827985E-5	12.184067886161625	1.7741893418413992E-5
74-75	1.7741893418413992E-5	1.3306420063810494E-4	3.5483786836827985E-5	12.859528381440773	1.7741893418413992E-5
76-77	1.7741893418413992E-5	1.4193514734731194E-4	3.5483786836827985E-5	13.55111625783726	1.7741893418413992E-5
78-79	1.7741893418413992E-5	1.4193514734731194E-4	3.5483786836827985E-5	14.288345155052617	1.7741893418413992E-5
80-81	1.7741893418413992E-5	1.5080609405651894E-4	3.5483786836827985E-5	15.037328056657671	1.7741893418413992E-5
82-83	1.7741893418413992E-5	1.5967704076572591E-4	3.5483786836827985E-5	15.76438197799757	1.7741893418413992E-5
84-85	1.7741893418413992E-5	1.8628988089334692E-4	3.5483786836827985E-5	16.460210166921055	1.7741893418413992E-5
86-87	1.7741893418413992E-5	2.483865078577959E-4	3.5483786836827985E-5	17.160793183280678	1.7741893418413992E-5
88	1.7741893418413992E-5	2.483865078577959E-4	3.5483786836827985E-5	17.70681769512579	1.7741893418413992E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5115	0.0	48.16054	1
GTATCAA	9130	0.0	37.89769	1
TCAACGC	9855	0.0	34.009785	4
ATCAACG	9995	0.0	33.58044	3
CAACGCA	10340	0.0	32.369377	5
AACGCAG	10830	0.0	30.901268	6
TATCAAC	11315	0.0	29.953785	2
ACGCAGA	12525	0.0	26.494255	7
CGCAGAG	12755	0.0	25.942808	8
GTACATG	13725	0.0	23.702787	1
TACATGG	14080	0.0	22.368824	2
GCAGAGT	14785	0.0	21.52247	9
ACATGGG	14320	0.0	21.27174	3
GTGGTAT	3270	0.0	19.120955	1
CATGGGG	10530	0.0	18.660313	4
AGAGTAC	12875	0.0	18.563868	10-11
CAGAGTA	14130	0.0	18.378704	10-11
GAGTACA	11000	0.0	17.052416	1
GAGTACT	6945	0.0	16.953382	12-13
TGGTATC	3350	0.0	15.996723	2
>>END_MODULE