FastQCFastQC Report
Wed 25 May 2016
SRR2049340_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049340_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5636377
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT176550.31323312830209904No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117700.2088220855347327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112040.19878017385991037No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT79150.14042708640674675TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61900.10982232025998262No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85450.057.273791
GTATCAA154350.041.241011
TCAACGC192700.032.7282184
ATCAACG192150.032.675153
CAACGCA200000.031.5571375
TATCAAC200850.031.4235842
AACGCAG207600.030.4795846
GTGGTAT38250.027.6546571
ACGCAGA238550.026.347847
TGGTATC38400.026.1899172
CGCAGAG242550.025.7775528
GTACATG132350.023.6219061
GCAGAGT262050.023.482149
TATACCG5600.022.6582985
TACATGG133300.022.2106842
GAGTACT193650.021.20938912-13
ACATGGG135850.021.1710953
CAGAGTA257700.020.82981110-11
AGAGTAC245850.020.32209210-11
GTACTTT215550.018.8372414-15
Produced by FastQC (version 0.11.5)