##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049340_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5636377 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.781617340358885 31.0 31.0 34.0 30.0 34.0 2 31.85735553175382 31.0 31.0 34.0 30.0 34.0 3 31.832923702584125 33.0 31.0 34.0 30.0 34.0 4 35.59925391789797 37.0 35.0 37.0 33.0 37.0 5 35.706178099868055 37.0 35.0 37.0 33.0 37.0 6 35.77232360432952 37.0 35.0 37.0 33.0 37.0 7 35.72617002020979 37.0 35.0 37.0 33.0 37.0 8 35.737036397671766 37.0 35.0 37.0 33.0 37.0 9 37.470658013117294 39.0 37.0 39.0 34.0 39.0 10-11 37.43590244229583 39.0 37.0 39.0 34.0 39.0 12-13 37.386484349787104 39.0 37.0 39.0 34.0 39.0 14-15 38.664171505915945 40.0 38.0 41.0 34.0 41.0 16-17 38.693446694569936 40.0 38.0 41.0 34.0 41.0 18-19 38.75934168349633 40.0 38.0 41.0 34.5 41.0 20-21 38.79132499476171 40.0 38.0 41.0 34.0 41.0 22-23 38.75411110718818 40.0 38.0 41.0 34.0 41.0 24-25 38.72710315509413 40.0 38.0 41.0 34.0 41.0 26-27 38.60445211525063 40.0 38.0 41.0 34.0 41.0 28-29 38.50918320403337 40.0 38.0 41.0 34.0 41.0 30-31 38.39837541030346 40.0 38.0 41.0 34.0 41.0 32-33 38.25407251857 40.0 38.0 41.0 34.0 41.0 34-35 38.138896049714205 40.0 38.0 41.0 33.5 41.0 36-37 38.00781104599639 40.0 38.0 41.0 33.0 41.0 38-39 37.886247051962634 40.0 37.0 41.0 33.0 41.0 40-41 37.77917188647956 40.0 37.0 41.0 33.0 41.0 42-43 37.64063351333667 40.0 37.0 41.0 33.0 41.0 44-45 37.46361598949112 40.0 37.0 41.0 32.5 41.0 46-47 37.238064008138565 40.0 37.0 41.0 32.0 41.0 48-49 37.034778812701845 40.0 37.0 41.0 31.0 41.0 50-51 36.879543100115555 40.0 36.0 41.0 31.0 41.0 52-53 36.745680780402026 40.0 36.0 41.0 31.0 41.0 54-55 36.58774333228597 39.0 36.0 41.0 31.0 41.0 56-57 36.43966310273426 39.0 35.0 41.0 30.5 41.0 58-59 36.23467575004298 39.0 35.0 41.0 30.0 41.0 60-61 36.028433335811286 39.0 35.0 41.0 30.0 41.0 62-63 35.80551709014496 38.5 35.0 41.0 29.5 41.0 64-65 35.549872462399165 38.0 35.0 40.0 29.0 41.0 66-67 35.234816443257785 37.5 35.0 40.0 29.0 41.0 68-69 34.88957028601884 37.0 34.0 40.0 28.0 41.0 70-71 34.513591443581575 36.5 34.0 39.5 28.0 41.0 72-73 34.2055134885406 36.0 34.0 39.0 28.0 41.0 74-75 33.88931338340214 35.5 34.0 39.0 27.5 40.5 76-77 33.55482662000785 35.0 34.0 37.5 27.0 40.0 78-79 33.21104931412501 35.0 33.5 37.0 26.5 39.0 80-81 32.88192725575312 35.0 33.0 37.0 26.0 39.0 82-83 32.5781210163905 35.0 33.0 36.0 26.0 39.0 84-85 32.30933638754115 35.0 33.0 36.0 26.0 37.0 86-87 32.07826809668693 35.0 33.0 35.5 25.5 37.0 88-89 31.844838182399794 35.0 33.0 35.0 25.0 36.5 90-91 31.64036108656323 35.0 33.0 35.0 25.0 36.0 92-93 31.44940606350498 35.0 33.0 35.0 25.0 36.0 94-95 31.289192330463344 35.0 33.0 35.0 24.0 36.0 96-97 31.081674274094865 35.0 32.5 35.0 23.5 35.5 98-99 30.91709337398829 35.0 32.5 35.0 21.5 35.0 100 30.791398446200457 35.0 32.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09711603483136244 1101 2 0.16678924365038839 1101 3 0.3169200850531162 1101 4 0.12005270577930816 1101 5 0.16969004805956445 1101 6 0.12524777590293468 1101 7 0.0012528410937164836 1101 8 0.07541046281192365 1101 9 0.24020866789131645 1101 10-11 0.21490576409841822 1101 12-13 0.14906059908908986 1101 14-15 0.27465714982670875 1101 16-17 0.19892576963555086 1101 18-19 0.2395308018681206 1101 20-21 0.2729729957256808 1101 22-23 0.2633965038619266 1101 24-25 0.2724760623794893 1101 26-27 0.347751884731494 1101 28-29 0.3319705713718406 1101 30-31 0.2971071616146972 1101 32-33 0.3584570003444085 1101 34-35 0.38653537684508166 1101 36-37 0.40406160855181383 1101 38-39 0.3618586361606546 1101 40-41 0.29943463301867723 1101 42-43 0.3470309942376417 1101 44-45 0.3963942299671501 1101 46-47 0.35191852547594493 1101 48-49 0.3706944791413562 1101 50-51 0.3448098808097413 1101 52-53 0.3913197853272834 1101 54-55 0.3071639303229361 1101 56-57 0.349044729686959 1101 58-59 0.32820674031015784 1101 60-61 0.3368264557049372 1101 62-63 0.287864955065011 1101 64-65 0.21993102728878 1101 66-67 0.2607878996726285 1101 68-69 0.3446772201942636 1101 70-71 0.37157737893611653 1101 72-73 0.3141542277719296 1101 74-75 0.41010322854886994 1101 76-77 0.3769488075218419 1101 78-79 0.44473989255227053 1101 80-81 0.4118843840282551 1101 82-83 0.37847160208941233 1101 84-85 0.3722000631596316 1101 86-87 0.2618986279075699 1101 88-89 0.3991622719570742 1101 90-91 0.43481522380544746 1101 92-93 0.43855075694119705 1101 94-95 0.4508369600338966 1101 96-97 0.459440575582569 1101 98-99 0.5885022175606167 1101 100 0.6102406160038036 1102 1 0.20119284391642722 1102 2 0.16370886302459908 1102 3 0.3059261522099881 1102 4 0.22379942042310574 1102 5 0.1367343926493092 1102 6 0.20320460399735651 1102 7 0.20139930266009998 1102 8 0.16347205720263247 1102 9 0.1512033669982884 1102 10-11 0.21307649699672027 1102 12-13 0.1648367695157873 1102 14-15 0.2714486078179519 1102 16-17 0.25691331138754236 1102 18-19 0.2542119662465865 1102 20-21 0.2277920515353884 1102 22-23 0.2544243055666655 1102 24-25 0.22805747558334843 1102 26-27 0.2944409563517638 1102 28-29 0.28471740936909384 1102 30-31 0.22136641361198173 1102 32-33 0.26421901892975796 1102 34-35 0.2642028577046389 1102 36-37 0.26234426705461544 1102 38-39 0.26874195416120017 1102 40-41 0.2611716239246249 1102 42-43 0.2145466061120871 1102 44-45 0.26399953978489776 1102 46-47 0.22289126436282913 1102 48-49 0.08117423392491219 1102 50-51 0.207185139442565 1102 52-53 0.21054560042541937 1102 54-55 0.16715408007643617 1102 56-57 0.21870745790774748 1102 58-59 0.23453466727649896 1102 60-61 0.21507704831986274 1102 62-63 0.23582925498308072 1102 64-65 0.19602547366390866 1102 66-67 0.17426987617056966 1102 68-69 0.17847902620300715 1102 70-71 0.11597783082142854 1102 72-73 0.12812752963736074 1102 74-75 0.10578800623694207 1102 76-77 0.14143250871440927 1102 78-79 0.21531283703826176 1102 80-81 0.183482619578065 1102 82-83 0.196258343651607 1102 84-85 0.20017586004021126 1102 86-87 0.22118274817133 1102 88-89 0.1945252038087233 1102 90-91 0.24898625179045197 1102 92-93 0.31600991921048305 1102 94-95 0.22627695699287997 1102 96-97 0.31746105165477445 1102 98-99 0.34976205146767114 1102 100 0.3946664086849623 1103 1 0.14332034932557747 1103 2 0.16494536317380337 1103 3 0.31610010920134 1103 4 0.1529140350951863 1103 5 0.19697159425888344 1103 6 0.12796868723143717 1103 7 0.13223706580246386 1103 8 0.15118278427066656 1103 9 0.19542783891987625 1103 10-11 0.18225487713068844 1103 12-13 0.18276475074392806 1103 14-15 0.26296065441417227 1103 16-17 0.1979737347212236 1103 18-19 0.23577249350920226 1103 20-21 0.25866803386045945 1103 22-23 0.25910518426351814 1103 24-25 0.23883467122480084 1103 26-27 0.2246059151211881 1103 28-29 0.3267920342777231 1103 30-31 0.2410349767059543 1103 32-33 0.2631183889582971 1103 34-35 0.3195506273282902 1103 36-37 0.3323238591908577 1103 38-39 0.2801460566252487 1103 40-41 0.2738013396581209 1103 42-43 0.30014745588341185 1103 44-45 0.39726168635338155 1103 46-47 0.3250580843570461 1103 48-49 0.30892284253851443 1103 50-51 0.39802681802484585 1103 52-53 0.3896414867867293 1103 54-55 0.3727302631517304 1103 56-57 0.33281483496298137 1103 58-59 0.4015576034861894 1103 60-61 0.3579478494493742 1103 62-63 0.374629130441015 1103 64-65 0.37007601065565154 1103 66-67 0.3575602566866749 1103 68-69 0.38207319766493697 1103 70-71 0.44102418638806995 1103 72-73 0.4675887134987349 1103 74-75 0.37955490404117853 1103 76-77 0.4130574968563394 1103 78-79 0.374881138204465 1103 80-81 0.4576057389655048 1103 82-83 0.476837262834799 1103 84-85 0.45511466666362566 1103 86-87 0.4237096936783189 1103 88-89 0.45280597077837115 1103 90-91 0.5182362455316927 1103 92-93 0.5146350933406083 1103 94-95 0.49245716238177906 1103 96-97 0.5523511416494671 1103 98-99 0.5793191050962498 1103 100 0.6443008349363275 1104 1 0.12540034842892567 1104 2 0.18781755608575068 1104 3 0.24327358942784727 1104 4 0.17573779070095696 1104 5 0.16244062656843994 1104 6 0.15614817304776807 1104 7 0.19056713893249366 1104 8 0.18128787262634205 1104 9 0.23747943298132412 1104 10-11 0.19813269576766146 1104 12-13 0.2107184911475315 1104 14-15 0.23127825622529485 1104 16-17 0.21121181862135785 1104 18-19 0.2455253329910363 1104 20-21 0.2922567384260617 1104 22-23 0.2354708535124388 1104 24-25 0.22642386107223444 1104 26-27 0.28507450743583007 1104 28-29 0.34826976894457573 1104 30-31 0.3126999856293011 1104 32-33 0.3461143045029118 1104 34-35 0.2885876094690403 1104 36-37 0.2830565226716857 1104 38-39 0.274557053378075 1104 40-41 0.310421631920768 1104 42-43 0.39214200206690464 1104 44-45 0.3861254907357292 1104 46-47 0.407946045899223 1104 48-49 0.405612073901473 1104 50-51 0.34889015469487106 1104 52-53 0.2985240981118835 1104 54-55 0.28541493038153476 1104 56-57 0.29112531658095975 1104 58-59 0.39797655225730466 1104 60-61 0.41548625737056 1104 62-63 0.4743850279253792 1104 64-65 0.3767732406869584 1104 66-67 0.4500747163130967 1104 68-69 0.4494746861351757 1104 70-71 0.4824243304883069 1104 72-73 0.4572285901547488 1104 74-75 0.4544628648290754 1104 76-77 0.41178000455776953 1104 78-79 0.4178934585165237 1104 80-81 0.4018098320844814 1104 82-83 0.4274388744733244 1104 84-85 0.4065980932440709 1104 86-87 0.39553437304622463 1104 88-89 0.38094133593401125 1104 90-91 0.3767220662600401 1104 92-93 0.49244583918273577 1104 94-95 0.5244189005449691 1104 96-97 0.5436294124588414 1104 98-99 0.54225612947954 1104 100 0.47514291856444046 1105 1 0.1893484684910014 1105 2 0.1472708969665355 1105 3 0.23829277813002747 1105 4 0.18452247260016463 1105 5 0.12948275595769587 1105 6 0.13425363145975666 1105 7 0.16377494729841402 1105 8 0.16305992071718833 1105 9 0.19191701177823006 1105 10-11 0.1983387612784071 1105 12-13 0.18992144027323832 1105 14-15 0.21966627192503552 1105 16-17 0.2541745859453286 1105 18-19 0.19211469476445586 1105 20-21 0.22767611723169523 1105 22-23 0.2020860068040662 1105 24-25 0.20479681910858716 1105 26-27 0.2194091147313202 1105 28-29 0.3027272732927102 1105 30-31 0.30037107783415706 1105 32-33 0.3085621710817037 1105 34-35 0.31910569902304786 1105 36-37 0.25075338477570597 1105 38-39 0.30924289805749794 1105 40-41 0.28196250398251976 1105 42-43 0.39377150139508643 1105 44-45 0.42825480792348003 1105 46-47 0.4505090410878978 1105 48-49 0.38134361727413335 1105 50-51 0.3795791067514074 1105 52-53 0.4252804381664532 1105 54-55 0.44009648007777 1105 56-57 0.36613745026880196 1105 58-59 0.3811201844943213 1105 60-61 0.4491010586320954 1105 62-63 0.4488460561775085 1105 64-65 0.3830642740808159 1105 66-67 0.39636969878545614 1105 68-69 0.40139998801808474 1105 70-71 0.40843384070528543 1105 72-73 0.44866525122328227 1105 74-75 0.3830265929033132 1105 76-77 0.3751003796769865 1105 78-79 0.4069123539203545 1105 80-81 0.38246529264035445 1105 82-83 0.39302344798905864 1105 84-85 0.3833828803559527 1105 86-87 0.36518120075794513 1105 88-89 0.43656136828330716 1105 90-91 0.4324740108028742 1105 92-93 0.495358248549401 1105 94-95 0.48984688012650324 1105 96-97 0.47751351493166183 1105 98-99 0.4993302946872227 1105 100 0.5540862229847079 1106 1 0.10776976590605614 1106 2 0.14455491802496923 1106 3 0.2748143336883082 1106 4 0.1700196038380568 1106 5 0.11215022300618216 1106 6 0.10119269529422326 1106 7 0.17485796377930996 1106 8 0.13597504415890427 1106 9 0.1514857059888115 1106 10-11 0.1755227540021025 1106 12-13 0.15968892852257 1106 14-15 0.2223397106696865 1106 16-17 0.2010050330627493 1106 18-19 0.2279454188572032 1106 20-21 0.22787730701354292 1106 22-23 0.18461101059784824 1106 24-25 0.21907807297639437 1106 26-27 0.2751614504423259 1106 28-29 0.2562799511399234 1106 30-31 0.2805545492292012 1106 32-33 0.16550579160708168 1106 34-35 0.2064721263024083 1106 36-37 0.23908506311391875 1106 38-39 0.22891226057581093 1106 40-41 0.21009760810176203 1106 42-43 0.2516145402584158 1106 44-45 0.24673197830639282 1106 46-47 0.27544930161647585 1106 48-49 0.29685815591628284 1106 50-51 0.2792640239382749 1106 52-53 0.22701656579923224 1106 54-55 0.30092148839430166 1106 56-57 0.2676723050063501 1106 58-59 0.3146409869761442 1106 60-61 0.3457511878145283 1106 62-63 0.23392413669345302 1106 64-65 0.17353392630466402 1106 66-67 0.24339525455594924 1106 68-69 0.27172822144532915 1106 70-71 0.253224747880175 1106 72-73 0.31274719574940235 1106 74-75 0.2700332431509338 1106 76-77 0.16406966921483956 1106 78-79 0.25321543973304017 1106 80-81 0.24580770126187446 1106 82-83 0.3149388594878886 1106 84-85 0.25881343015993963 1106 86-87 0.2892181158414928 1106 88-89 0.2560105703914246 1106 90-91 0.2611818492047071 1106 92-93 0.3621729470670516 1106 94-95 0.3245881125136556 1106 96-97 0.34341194387412344 1106 98-99 0.3479600078996654 1106 100 0.34203403526399967 1107 1 0.1047650421390891 1107 2 0.1232993173555279 1107 3 0.21159526603337397 1107 4 0.18931182783668987 1107 5 0.11949134170699693 1107 6 0.16485367322455602 1107 7 0.11338921347230269 1107 8 0.12110547099106128 1107 9 0.17926058863449157 1107 10-11 0.17615081735145566 1107 12-13 0.18955563090653982 1107 14-15 0.2169750656125018 1107 16-17 0.1733562952984542 1107 18-19 0.13119627297940895 1107 20-21 0.14435689662792583 1107 22-23 0.11263453681982583 1107 24-25 0.13188284958013696 1107 26-27 0.2298169821151106 1107 28-29 0.24797260646104746 1107 30-31 0.21444550583598954 1107 32-33 0.27362880832119885 1107 34-35 0.2198945881309129 1107 36-37 0.2895945098213133 1107 38-39 0.26782196238455924 1107 40-41 0.30823749256106225 1107 42-43 0.3231604670145316 1107 44-45 0.28081134988608625 1107 46-47 0.3150819307896171 1107 48-49 0.3280851492758856 1107 50-51 0.3066644299269612 1107 52-53 0.28075574347626286 1107 54-55 0.2646793557001814 1107 56-57 0.21012536223695832 1107 58-59 0.26188472064583124 1107 60-61 0.20669809397691097 1107 62-63 0.2579932748939555 1107 64-65 0.23467501709663452 1107 66-67 0.2332142027831523 1107 68-69 0.21724052724407272 1107 70-71 0.21741440537881118 1107 72-73 0.26866670289680883 1107 74-75 0.2909320470687149 1107 76-77 0.2556568472018341 1107 78-79 0.2680694228665388 1107 80-81 0.31267822375529164 1107 82-83 0.31328999832041404 1107 84-85 0.2865592024483803 1107 86-87 0.31560108393325237 1107 88-89 0.2914184144930161 1107 90-91 0.3511210799227946 1107 92-93 0.38042256063915403 1107 94-95 0.38955895572230403 1107 96-97 0.37993147838204067 1107 98-99 0.3962659107691593 1107 100 0.4086867542257977 1108 1 0.05049364926355793 1108 2 0.015787918551005475 1108 3 0.11358983528438671 1108 4 0.039726681327479696 1108 5 0.041194855364551586 1108 6 0.007250037183645475 1108 7 0.02158658834698457 1108 8 -0.04036488700597829 1108 9 0.05259237444708731 1108 10-11 0.07239535359540383 1108 12-13 0.04006742460100554 1108 14-15 0.08945464852800455 1108 16-17 -0.014143821694908354 1108 18-19 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14-15 -0.3887299036758307 2215 16-17 -0.4124861676935083 2215 18-19 -0.4073386952051834 2215 20-21 -0.42720010271507647 2215 22-23 -0.3723213665706169 2215 24-25 -0.3723898805503225 2215 26-27 -0.4517045230808918 2215 28-29 -0.3893085361165092 2215 30-31 -0.387894424881452 2215 32-33 -0.34276807910757867 2215 34-35 -0.4015911155952594 2215 36-37 -0.3716949696843841 2215 38-39 -0.3732018252924689 2215 40-41 -0.371268067405488 2215 42-43 -0.33423096674578545 2215 44-45 -0.4344405573893724 2215 46-47 -0.45654401005860024 2215 48-49 -0.4168014685108403 2215 50-51 -0.45235242654278807 2215 52-53 -0.4425401280163186 2215 54-55 -0.27309421549227153 2215 56-57 -0.363213055430883 2215 58-59 -0.423033398667755 2215 60-61 -0.4405179500362095 2215 62-63 -0.42230548013939284 2215 64-65 -0.38732227255962925 2215 66-67 -0.34983090218258894 2215 68-69 -0.3809241288785543 2215 70-71 -0.47900390048348385 2215 72-73 -0.48499696234392786 2215 74-75 -0.48090909588736963 2215 76-77 -0.3635280227987323 2215 78-79 -0.46205796923879205 2215 80-81 -0.5540312510449894 2215 82-83 -0.4765599871473398 2215 84-85 -0.5008923063661612 2215 86-87 -0.4761607563814323 2215 88-89 -0.5182972453665755 2215 90-91 -0.5302582872938792 2215 92-93 -0.4923207598678232 2215 94-95 -0.47705303426234735 2215 96-97 -0.5767201506257109 2215 98-99 -0.5419162311322872 2215 100 -0.4890859259178484 2216 1 -0.39220224275902993 2216 2 -0.34583175460205595 2216 3 -0.45184986364556323 2216 4 -0.26737154424604626 2216 5 -0.4768509202666067 2216 6 -0.3812877948541882 2216 7 -0.6190422544950493 2216 8 -0.3725105027157696 2216 9 -0.6436412692733455 2216 10-11 -0.4526153549178247 2216 12-13 -0.39898167137111074 2216 14-15 -0.7245227581965281 2216 16-17 -0.5868955700153649 2216 18-19 -0.5166721812306889 2216 20-21 -0.5107838531062896 2216 22-23 -0.5448848830569943 2216 24-25 -0.538441410026735 2216 26-27 -0.6938070980729023 2216 28-29 -0.735211118426939 2216 30-31 -0.7807557623468355 2216 32-33 -0.7369038976978075 2216 34-35 -0.7536746165021455 2216 36-37 -0.7985333136564208 2216 38-39 -0.6973598694044085 2216 40-41 -0.739463058554243 2216 42-43 -0.7612239948010142 2216 44-45 -1.006954415194599 2216 46-47 -0.9158703115001003 2216 48-49 -1.0541265712639856 2216 50-51 -0.9317756388218328 2216 52-53 -0.8453192477876925 2216 54-55 -0.8197095186541077 2216 56-57 -0.890721280446364 2216 58-59 -1.035468897607764 2216 60-61 -0.8955919884549388 2216 62-63 -0.7462830507049247 2216 64-65 -0.8863138697686423 2216 66-67 -0.7273401948207123 2216 68-69 -0.7480836209102293 2216 70-71 -0.7677277912604339 2216 72-73 -0.7624303641905996 2216 74-75 -0.8032192095557704 2216 76-77 -0.7056300318348079 2216 78-79 -1.0249017110603589 2216 80-81 -1.1356297660777912 2216 82-83 -1.0111020326777655 2216 84-85 -1.0914635679542428 2216 86-87 -0.9489664840915815 2216 88-89 -1.2962296053484437 2216 90-91 -1.0705779103288648 2216 92-93 -1.149650407763552 2216 94-95 -1.1544286358829936 2216 96-97 -1.2466728976261692 2216 98-99 -1.3779108536866858 2216 100 -1.2826221547702765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 270.0 3 27.0 4 40.0 5 90.0 6 298.0 7 801.0 8 1951.0 9 3496.0 10 5343.0 11 7638.0 12 10833.0 13 14325.0 14 17351.0 15 21858.0 16 26199.0 17 34329.0 18 33280.0 19 20244.0 20 17235.0 21 17868.0 22 19792.0 23 22829.0 24 26278.0 25 32150.0 26 38476.0 27 43251.0 28 54253.0 29 71113.0 30 92695.0 31 119590.0 32 153153.0 33 200523.0 34 271825.0 35 390998.0 36 628582.0 37 1116111.0 38 1521707.0 39 597390.0 40 2185.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.03787004953724 20.81533139510222 14.334988597613885 23.811809957746657 2 15.572716303398442 20.859179575816167 39.55789330628522 24.010210814500166 3 17.8086739052409 25.389022061512208 31.11360365000425 25.68870038324264 4 12.670433154364847 17.57369188063106 37.880559380906945 31.87531558409715 5 12.559787963083377 37.593191512916896 35.87279559192013 13.974224932079595 6 30.5387007538177 38.33931590085544 17.109539888751215 14.012443456575644 7 26.07093173504895 32.94862994437739 22.499045042586754 18.481393277986903 8 24.554492194465148 36.32250540733929 20.544039276684206 18.578963121511354 9 24.402288917153697 16.390759525134673 21.69570985049439 37.51124170721724 10-11 23.58706665647099 26.91634182738309 28.908472587976284 20.58811892816964 12-13 24.322721331811945 24.390259054647604 29.09000595237197 22.19701366116848 14-15 21.609116216304685 26.51396327798362 26.625959049732955 25.250961455978743 16-17 20.77411791068251 29.884483408799962 27.3190320057576 22.022366674759926 18-19 20.576138185306565 28.10934193768387 30.197386670702357 21.117133206307212 20-21 21.590146995265748 27.326718321643778 30.032412693839227 21.050721989251244 22-23 21.252189127874168 27.457877640193335 30.019265922063056 21.27066730986944 24-25 21.29464342345841 27.552748705926977 30.003669914885826 21.14893795572878 26-27 21.080659590898225 27.40889641342903 30.1398372800946 21.37060671557815 28-29 20.886069492637304 27.29963216459581 30.34517447232053 21.469123870446357 30-31 21.341450486850906 27.56559999141292 30.067074121113098 21.02587540062308 32-33 20.91030711922931 27.546849150821856 30.250428401372055 21.292415328576777 34-35 21.001944336169494 27.195149148272886 30.48672629959951 21.316180215958106 36-37 21.50049083017808 27.406207790866784 29.76235232292279 21.33094905603235 38-39 21.237687637805088 27.24290465961394 30.061515792779293 21.457891909801678 40-41 20.996306575112428 26.933046860411423 30.372088051391255 21.69855851308489 42-43 21.051138450336442 26.906705159690336 30.29234720508642 21.749809184886804 44-45 20.943318278991658 27.179516703914413 30.09443952154569 21.782725495548235 46-47 21.150930678953063 27.32838286492598 29.53382754038992 21.98685891573103 48-49 21.00714800043255 27.186042907518836 29.457440535568214 22.3493685564804 50-51 20.85038634973309 27.220402090433087 29.420525077958338 22.508686481875483 52-53 20.775816735103465 27.290864432688995 29.54256340634993 22.390755425857613 54-55 20.789382336261088 27.602620386780895 29.12271049484475 22.485286782113263 56-57 20.843375310928725 27.402507655677265 28.89747602167364 22.85664101172037 58-59 20.539604677977856 27.219678917288388 29.27234866004999 22.968367744683764 60-61 20.856400077993584 27.758841209780567 28.501474621657486 22.883284090568363 62-63 20.5205735691266 27.882405217658363 28.424061686332124 23.172959526882913 64-65 20.488355875589846 27.62511420308975 28.482993530997643 23.403536390322763 66-67 20.59420657812259 27.702411994842706 28.184289343573404 23.5190920834613 68-69 21.002174215624812 27.70545423932127 27.931675923797684 23.360695621256234 70-71 20.955615146934225 27.48162218854991 27.925837091159284 23.636925573356578 72-73 20.76928256246788 27.457134460068023 28.066468588128433 23.707114389335658 74-75 20.849583234014627 27.41664702470133 27.975912992111073 23.757856749172976 76-77 20.902823371131866 27.439896833568728 27.812595058539426 23.844684736759977 78-79 21.008627379036295 27.44377867686908 27.819493843751918 23.728100100342704 80-81 20.88581559711151 27.256342205369744 27.906448805890534 23.951393391628216 82-83 20.68879223171298 27.16436960733375 28.065412764986537 24.081425395966736 84-85 20.77555927810793 26.987669831348743 27.996600201571393 24.240170688971936 86-87 20.92346247419068 27.130730999793744 27.906658416555224 24.03914810946035 88-89 20.770088470877372 26.88714851147343 27.97862554815812 24.36413746949108 90-91 20.691593069751193 26.59817689837815 28.04409303470997 24.666136997160685 92-93 20.735958451649005 26.611509806602058 28.030463988463204 24.62206775328573 94-95 20.551110306782046 26.334561561557567 28.358800550196566 24.755527581463824 96-97 20.62891438070249 26.34705173540594 28.32343169501872 24.700602188872853 98-99 20.669274923334928 26.4085632812439 28.242448286115973 24.6797135093052 100 20.6954776529776 26.39352525478155 28.211988043927406 24.699009048313442 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 594.0 1 434.0 2 545.5 3 810.0 4 522.0 5 323.5 6 784.0 7 1919.0 8 3271.5 9 3771.5 10 2980.0 11 2114.5 12 2065.0 13 2387.5 14 2817.5 15 3319.0 16 3842.5 17 4456.0 18 5265.5 19 6162.5 20 7470.5 21 9322.0 22 11905.5 23 15735.0 24 21023.5 25 27879.5 26 37120.0 27 48410.0 28 61914.5 29 77753.0 30 93310.0 31 107090.5 32 118546.5 33 128976.0 34 141067.0 35 152127.5 36 161447.0 37 172063.0 38 183040.0 39 190572.5 40 196236.0 41 202908.0 42 206132.0 43 208220.5 44 209793.5 45 208149.0 46 204805.0 47 198327.0 48 191674.0 49 185897.0 50 180250.0 51 173651.5 52 163641.0 53 155299.5 54 147386.0 55 137509.0 56 126012.5 57 112435.5 58 100226.5 59 89395.0 60 77642.0 61 65317.0 62 55185.5 63 46423.5 64 37879.0 65 31010.0 66 25309.5 67 19838.5 68 15417.0 69 12229.0 70 9542.0 71 7183.0 72 5318.0 73 4040.5 74 3029.5 75 2167.0 76 1549.5 77 1182.0 78 1026.0 79 1012.0 80 705.0 81 259.5 82 134.0 83 76.0 84 39.5 85 18.5 86 11.5 87 9.0 88 6.5 89 2.0 90 0.0 91 1.0 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0360160436393804 2 0.0 3 0.0 4 5.3225680255241974E-5 5 0.0 6 1.7741893418413992E-5 7 0.0 8 9.935460314311835E-4 9 0.0 10-11 0.0 12-13 0.04415070177172322 14-15 6.209662696444897E-5 16-17 8.870946709206996E-6 18-19 6.209662696444897E-5 20-21 8.870946709206996E-5 22-23 0.0 24-25 1.1532230721969094E-4 26-27 7.983852038286296E-5 28-29 4.3467638875114276E-4 30-31 8.870946709206996E-6 32-33 2.749993479854169E-4 34-35 1.0645136051048395E-4 36-37 1.4193514734731194E-4 38-39 3.459669216590728E-4 40-41 1.6854798747493292E-4 42-43 5.499986959708338E-4 44-45 3.2822502824065886E-4 46-47 1.4193514734731194E-4 48-49 8.072561505378367E-4 50-51 5.854824828076618E-4 52-53 5.854824828076618E-4 54-55 5.943534295168687E-4 56-57 6.564500564813177E-4 58-59 1.7741893418413992E-4 60-61 2.838702946946239E-4 62-63 9.846750847219766E-4 64-65 6.475791097721107E-4 66-67 0.0015612866208204311 68-69 0.0016233832477848804 70-71 5.056439624247988E-4 72-73 0.0010733845518140466 74-75 0.0010733845518140466 76-77 8.427399373746647E-4 78-79 0.0034153144830446937 80-81 0.0026169292792160635 82-83 0.0013572548465086702 84-85 0.0028475738936554456 86-87 0.0015878994609480523 88-89 0.0020491886898268163 90-91 0.0028032191601094106 92-93 0.0026346711726344778 94-95 0.003716926671157731 96-97 0.0033443469093710375 98-99 0.0030693475613856208 100 0.0013838676866362912 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5636377.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.524934414382315 #Duplication Level Percentage of deduplicated Percentage of total 1 77.04072623239693 48.16966354917001 2 13.97401213643108 17.474483846722716 3 4.165693247312202 7.813790912358922 4 1.7921977780385943 4.482281941178592 5 0.8885754038832503 2.777905942501675 6 0.5231357674930542 1.962541773139246 7 0.32969281575004317 1.4429815177165135 8 0.2287434379348424 1.144173476367708 9 0.163122400956848 0.9179295679209146 >10 0.8396190981453957 9.110222981027166 >50 0.04048749195290696 1.7255642580791992 >100 0.013058672638872762 1.3913352542066895 >500 4.535865739054946E-4 0.2122420385355026 >1k 3.4018622984873816E-4 0.40471724064846243 >5k 5.6697704918229185E-5 0.2500125553498453 >10k+ 8.504655737734377E-5 0.7201531450768426 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 17655 0.31323312830209904 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11770 0.2088220855347327 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11204 0.19878017385991037 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 7915 0.14042708640674675 TruSeq Adapter, Index 11 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6190 0.10982232025998262 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.7741893418413992E-5 0.0 1.7741893418413992E-5 0.0 5 0.0 3.5483786836827985E-5 0.0 3.5483786836827985E-5 0.0 6 0.0 3.5483786836827985E-5 0.0 3.5483786836827985E-5 0.0 7 0.0 3.5483786836827985E-5 0.0 3.5483786836827985E-5 0.0 8 0.0 3.5483786836827985E-5 0.0 3.5483786836827985E-5 0.0 9 0.0 3.5483786836827985E-5 0.0 1.2419325392889794E-4 0.0 10-11 0.0 3.5483786836827985E-5 0.0 1.7741893418413992E-4 0.0 12-13 0.0 3.5483786836827985E-5 0.0 2.129027210209679E-4 0.0 14-15 0.0 3.5483786836827985E-5 0.0 2.395155611485889E-4 0.0 16-17 0.0 3.5483786836827985E-5 0.0 2.749993479854169E-4 0.0 18-19 0.0 3.5483786836827985E-5 0.0 3.5483786836827983E-4 0.0 20-21 0.0 3.5483786836827985E-5 0.0 6.475791097721107E-4 0.0 22-23 0.0 3.5483786836827985E-5 0.0 0.0016411251412032943 0.0 24-25 0.0 5.3225680255241974E-5 0.0 0.004568537555241603 0.0 26-27 0.0 5.3225680255241974E-5 0.0 0.010600781317502361 0.0 28-29 0.0 5.3225680255241974E-5 1.7741893418413992E-5 0.03756845931349163 0.0 30-31 0.0 5.3225680255241974E-5 1.7741893418413992E-5 0.1180545588061267 0.0 32-33 0.0 6.209662696444897E-5 1.7741893418413992E-5 0.29473720441340245 0.0 34-35 0.0 7.096757367365597E-5 1.7741893418413992E-5 0.5675099447748084 0.0 36-37 0.0 7.096757367365597E-5 1.7741893418413992E-5 0.9793259038563247 0.0 38-39 0.0 7.096757367365597E-5 1.7741893418413992E-5 1.4907625944822356 0.0 40-41 0.0 7.096757367365597E-5 1.7741893418413992E-5 2.0007267079544184 0.0 42-43 0.0 7.983852038286296E-5 1.7741893418413992E-5 2.4917247373623166 0.0 44-45 0.0 8.870946709206996E-5 1.7741893418413992E-5 3.06610966583676 0.0 46-47 0.0 1.0645136051048395E-4 1.7741893418413992E-5 3.7104242672198824 0.0 48-49 0.0 1.0645136051048395E-4 1.7741893418413992E-5 4.351820327135676 0.0 50-51 0.0 1.0645136051048395E-4 1.7741893418413992E-5 4.965104356929992 0.0 52-53 0.0 1.1532230721969094E-4 1.7741893418413992E-5 5.529190116275046 0.0 54-55 0.0 1.2419325392889794E-4 1.7741893418413992E-5 6.1279878900932285 0.0 56-57 0.0 1.2419325392889794E-4 1.7741893418413992E-5 6.800414521597828 0.0 58-59 0.0 1.2419325392889794E-4 3.5483786836827985E-5 7.484533060865163 0.0 60-61 0.0 1.2419325392889794E-4 3.5483786836827985E-5 8.151078254701558 0.0 62-63 0.0 1.3306420063810494E-4 3.5483786836827985E-5 8.771875976358572 0.0 64-65 0.0 1.5080609405651894E-4 5.3225680255241974E-5 9.411914781427857 0.0 66-67 0.0 1.5967704076572591E-4 5.3225680255241974E-5 10.10610184521014 0.0 68-69 0.0 1.5967704076572591E-4 5.3225680255241974E-5 10.82754045728311 0.0 70-71 0.0 1.5967704076572591E-4 5.3225680255241974E-5 11.55387583193956 0.0 72-73 0.0 1.5967704076572591E-4 5.3225680255241974E-5 12.261466896199456 0.0 74-75 0.0 1.7741893418413992E-4 5.3225680255241974E-5 12.943607214350637 0.0 76-77 0.0 1.7741893418413992E-4 5.3225680255241974E-5 13.64161765616459 0.0 78-79 0.0 1.7741893418413992E-4 5.3225680255241974E-5 14.386688470270885 0.0 80-81 0.0 1.7741893418413992E-4 5.3225680255241974E-5 15.143211676578767 0.0 82-83 0.0 1.7741893418413992E-4 5.3225680255241974E-5 15.877601870847178 0.0 84-85 0.0 1.9516082760255392E-4 5.3225680255241974E-5 16.57985262518813 0.0 86-87 0.0 1.9516082760255392E-4 5.3225680255241974E-5 17.287239657673716 0.0 88 0.0 1.9516082760255392E-4 5.3225680255241974E-5 17.83913673624032 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8545 0.0 57.27379 1 GTATCAA 15435 0.0 41.24101 1 TCAACGC 19270 0.0 32.728218 4 ATCAACG 19215 0.0 32.67515 3 CAACGCA 20000 0.0 31.557137 5 TATCAAC 20085 0.0 31.423584 2 AACGCAG 20760 0.0 30.479584 6 GTGGTAT 3825 0.0 27.654657 1 ACGCAGA 23855 0.0 26.34784 7 TGGTATC 3840 0.0 26.189917 2 CGCAGAG 24255 0.0 25.777552 8 GTACATG 13235 0.0 23.621906 1 GCAGAGT 26205 0.0 23.48214 9 TATACCG 560 0.0 22.658298 5 TACATGG 13330 0.0 22.210684 2 GAGTACT 19365 0.0 21.209389 12-13 ACATGGG 13585 0.0 21.171095 3 CAGAGTA 25770 0.0 20.829811 10-11 AGAGTAC 24585 0.0 20.322092 10-11 GTACTTT 21555 0.0 18.83724 14-15 >>END_MODULE