FastQCFastQC Report
Wed 25 May 2016
SRR2049339_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049339_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9972985
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG14850.042.1202132
TAACGCA15800.038.099224
TACCTGG108100.037.1968152
ACCTGGG127550.030.9347673
GTACCTG136950.030.2194121
GGTATCA90450.029.7928071
GTATCAA130250.029.4269491
GTATAAC32900.026.1589661
TCAACGC138250.025.9891094
ATAACGC26400.025.83033
GTACATG308350.025.7603071
ATCAACG144150.024.925513
CAACGCA149500.024.2536095
TACATGG319450.024.0407872
TATCACG18650.023.955772
ACATGGG322700.022.9388243
AACGCAG161550.022.6338756
TATCAAC169450.021.7590472
CCTGGGG138400.020.999824
ACGCAGA183900.019.753937
Produced by FastQC (version 0.11.5)