##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049339_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9972985 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.135457337998602 31.0 30.0 33.0 25.0 34.0 2 29.26201282765391 31.0 30.0 34.0 25.0 34.0 3 29.345928425641873 31.0 30.0 34.0 25.0 34.0 4 32.96341656986348 35.0 33.0 37.0 26.0 37.0 5 32.939341130062864 35.0 33.0 37.0 26.0 37.0 6 32.97158724293679 35.0 33.0 37.0 26.0 37.0 7 32.832902786878755 35.0 33.0 37.0 26.0 37.0 8 32.770403444906414 35.0 33.0 37.0 26.0 37.0 9 34.01935408506079 37.0 34.0 39.0 25.0 39.0 10-11 33.91745786241532 37.0 33.5 39.0 25.0 39.0 12-13 33.80477324492115 37.0 33.0 39.0 24.0 39.0 14-15 34.5785145069405 38.0 33.0 40.0 22.5 41.0 16-17 34.59794815694599 38.0 33.0 40.0 23.5 41.0 18-19 34.642989486096695 38.5 33.5 40.0 23.5 41.0 20-21 34.7672301723105 39.0 34.0 40.0 24.0 41.0 22-23 34.68913650226085 39.0 34.0 40.0 24.0 41.0 24-25 34.585330971619825 38.0 34.0 40.0 24.0 41.0 26-27 34.406255048012206 38.0 33.0 40.0 23.0 41.0 28-29 34.295413660002495 38.0 33.0 40.0 22.0 41.0 30-31 34.25840733742204 38.0 33.0 40.0 22.5 41.0 32-33 34.14096546821237 38.0 33.0 40.0 21.5 41.0 34-35 34.06744209481916 38.0 33.0 40.0 21.5 41.0 36-37 33.94461116706783 38.0 33.0 40.0 20.0 41.0 38-39 33.790436464107785 38.0 33.0 40.0 18.5 41.0 40-41 33.740817217713655 38.0 33.0 40.0 19.5 41.0 42-43 33.58918247646016 38.0 32.5 40.0 17.5 41.0 44-45 33.469883640655226 38.0 32.0 40.0 17.5 41.0 46-47 33.21900699740348 38.0 32.0 40.0 15.0 41.0 48-49 33.13466113706177 38.0 32.0 40.0 15.0 41.0 50-51 33.04070531540958 37.5 32.0 40.0 15.0 41.0 52-53 32.85539459850787 37.0 32.0 40.0 13.5 41.0 54-55 32.77116861200533 37.0 32.0 40.0 13.0 41.0 56-57 32.53649784893891 37.0 31.0 40.0 11.5 41.0 58-59 32.34431817555125 36.0 31.0 40.0 8.5 41.0 60-61 32.056172199196126 36.0 31.0 39.5 8.0 41.0 62-63 31.75588056133645 35.5 31.0 39.0 7.0 41.0 64-65 31.54662450610324 35.0 31.0 39.0 6.0 40.5 66-67 31.250794872347647 35.0 30.0 39.0 2.0 40.0 68-69 30.960489562553235 35.0 30.0 38.0 2.0 40.0 70-71 30.67531862326074 35.0 30.0 37.5 2.0 40.0 72-73 30.336642940904852 35.0 29.5 37.0 2.0 39.5 74-75 29.983439963060206 34.0 29.0 36.5 2.0 39.0 76-77 29.714651430840412 34.0 29.0 36.0 2.0 39.0 78-79 29.413506136828644 34.0 29.0 36.0 2.0 38.5 80-81 29.083878698303465 34.0 29.0 35.0 2.0 37.0 82-83 28.796744906364545 34.0 29.0 35.0 2.0 37.0 84-85 28.539056210352264 34.0 29.0 35.0 2.0 36.5 86-87 28.321243389015425 34.0 29.0 35.0 2.0 36.0 88-89 28.06632442543531 34.0 28.0 35.0 2.0 36.0 90-91 27.74862470965313 34.0 27.0 35.0 2.0 35.0 92-93 27.551349119646723 34.0 27.0 35.0 2.0 35.0 94-95 27.287586514970194 34.0 27.0 35.0 2.0 35.0 96-97 27.008709127708507 33.5 26.5 35.0 2.0 35.0 98-99 26.70878011949281 33.0 25.5 35.0 2.0 35.0 100 26.515581042185463 33.0 25.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5656723335770977 1101 2 0.5290799055236732 1101 3 0.5315626714215682 1101 4 0.5447646063723894 1101 5 0.46961366744831423 1101 6 0.5113004543110407 1101 7 0.5621949254857057 1101 8 0.5946063967463857 1101 9 0.6737596023758883 1101 10-11 0.6613960209585841 1101 12-13 0.6570675241594017 1101 14-15 0.8878417491120985 1101 16-17 0.8865876133366513 1101 18-19 0.8433004426723159 1101 20-21 0.6994797918557083 1101 22-23 0.6162342230432643 1101 24-25 0.6072217276188212 1101 26-27 0.7462133242415732 1101 28-29 0.8483835052160131 1101 30-31 0.8159157526946146 1101 32-33 0.8784220497848523 1101 34-35 0.9025304403661778 1101 36-37 0.8743686062915188 1101 38-39 0.9114509792994312 1101 40-41 0.8707678036824618 1101 42-43 0.9231317973729602 1101 44-45 0.8688872355058024 1101 46-47 0.9223715099901284 1101 48-49 0.8423828449905741 1101 50-51 0.7952212657949929 1101 52-53 0.7902463474663293 1101 54-55 0.6079887930149326 1101 56-57 0.689568428957358 1101 58-59 0.5994877333387123 1101 60-61 0.6727479049707412 1101 62-63 0.7450333107837395 1101 64-65 0.785871536611122 1101 66-67 0.8249120561536678 1101 68-69 0.6666090264089632 1101 70-71 0.6273859437279583 1101 72-73 0.5719680899454822 1101 74-75 0.6329025611138235 1101 76-77 0.6176697796324717 1101 78-79 0.5689465544248264 1101 80-81 0.6191895098726725 1101 82-83 0.7105681473451533 1101 84-85 0.6706605704092823 1101 86-87 0.6324128615201587 1101 88-89 0.7318000745402315 1101 90-91 0.778567513010664 1101 92-93 0.7186242346651639 1101 94-95 0.6629944893545847 1101 96-97 0.7111527419051562 1101 98-99 0.707025258186377 1101 100 0.7694766662363755 1102 1 0.48042808088201383 1102 2 0.5389278906190036 1102 3 0.5903234145542093 1102 4 0.5519748210138431 1102 5 0.5609614861540777 1102 6 0.4259664689310938 1102 7 0.45810242312825267 1102 8 0.5409999709490521 1102 9 0.5700923359658105 1102 10-11 0.6679443412738735 1102 12-13 0.6833811027903991 1102 14-15 0.9188961773519893 1102 16-17 0.8320491825766112 1102 18-19 0.7043013402170928 1102 20-21 0.6141794494222736 1102 22-23 0.6800448133088892 1102 24-25 0.6486819055935555 1102 26-27 0.7991180017607107 1102 28-29 0.7980532486186718 1102 30-31 0.7927614238090186 1102 32-33 0.8563144660163644 1102 34-35 0.7823764548293184 1102 36-37 0.8244228463488383 1102 38-39 0.8730361417738948 1102 40-41 0.7216841667181271 1102 42-43 0.8165901974745964 1102 44-45 0.7042365032282518 1102 46-47 0.7918994915506374 1102 48-49 0.8001306684690022 1102 50-51 0.6966320275933455 1102 52-53 0.7508941112101439 1102 54-55 0.5852147928724065 1102 56-57 0.5540349722320528 1102 58-59 0.518369794843359 1102 60-61 0.46847537062551936 1102 62-63 0.5679099941024361 1102 64-65 0.5304650878657533 1102 66-67 0.5779397442714043 1102 68-69 0.6459369749094819 1102 70-71 0.5643928770625095 1102 72-73 0.5306598636757194 1102 74-75 0.551487624865981 1102 76-77 0.5532565311436528 1102 78-79 0.5573967129580168 1102 80-81 0.5517823387982617 1102 82-83 0.5758379398038578 1102 84-85 0.5734472061342473 1102 86-87 0.5860903243309465 1102 88-89 0.593049643856034 1102 90-91 0.6617393029941212 1102 92-93 0.5630979462029693 1102 94-95 0.557046775996195 1102 96-97 0.413874036762504 1102 98-99 0.3974870769562102 1102 100 0.3528597965787945 1103 1 0.12629603839176085 1103 2 0.17463583737172428 1103 3 0.22791921931419523 1103 4 0.07296536379543284 1103 5 0.1789185476932289 1103 6 0.1033193441944178 1103 7 0.08636740452755731 1103 8 0.12546592944485724 1103 9 0.20054906134790684 1103 10-11 0.22869912590925168 1103 12-13 0.2949404233049506 1103 14-15 0.48721584029851783 1103 16-17 0.3375684331704889 1103 18-19 0.22933069793301541 1103 20-21 0.14921777132627057 1103 22-23 0.2828196777255485 1103 24-25 0.25427555894840026 1103 26-27 0.33618760868106534 1103 28-29 0.28803980233657 1103 30-31 0.23392004511689635 1103 32-33 0.28889480755193375 1103 34-35 0.27491197542454415 1103 36-37 0.20732052014203362 1103 38-39 0.2696342324018275 1103 40-41 0.2336222241785748 1103 42-43 0.22020769556971231 1103 44-45 0.1263489141466323 1103 46-47 0.23934111227583799 1103 48-49 0.17502466694508456 1103 50-51 0.18471906985949005 1103 52-53 0.21234362042084598 1103 54-55 0.058549919043358045 1103 56-57 0.08790615684950609 1103 58-59 -0.008785420073046168 1103 60-61 0.17933279521428602 1103 62-63 0.07098426240059297 1103 64-65 0.10777269397933154 1103 66-67 0.16731673320063223 1103 68-69 0.10909998866951298 1103 70-71 0.05080720183504539 1103 72-73 0.0551137205176353 1103 74-75 0.05828337584083698 1103 76-77 0.07086936786096487 1103 78-79 0.08554381528422184 1103 80-81 0.09645268078203628 1103 82-83 0.04943302742879396 1103 84-85 0.025844678233212903 1103 86-87 0.020072664880451896 1103 88-89 -0.0381950336573027 1103 90-91 0.045594352073049293 1103 92-93 -0.027456040619675548 1103 94-95 -0.021745747870955512 1103 96-97 -0.04454871434681351 1103 98-99 7.782786056225177E-4 1103 100 -0.11380866292548575 1104 1 0.1561550591873626 1104 2 0.20733775664565002 1104 3 0.2520635343498938 1104 4 0.2314141458613932 1104 5 0.11355825015719745 1104 6 0.135520138514309 1104 7 0.14968989597547733 1104 8 0.1266142846007412 1104 9 0.301848053659711 1104 10-11 0.31253086071500746 1104 12-13 0.300472441127269 1104 14-15 0.5259972383986735 1104 16-17 0.39901822092974015 1104 18-19 0.2750317992950997 1104 20-21 0.21085872320911392 1104 22-23 0.19251375658161862 1104 24-25 0.29635435820524947 1104 26-27 0.3670700709339556 1104 28-29 0.28910006066833915 1104 30-31 0.27471269102264984 1104 32-33 0.350561565290441 1104 34-35 0.32330677140627984 1104 36-37 0.2727002181478255 1104 38-39 0.304999541057299 1104 40-41 0.26707623853843643 1104 42-43 0.20856542097683928 1104 44-45 0.21033487477237145 1104 46-47 0.3835688209257597 1104 48-49 0.31677808170531563 1104 50-51 0.3237847161746359 1104 52-53 0.31034996032175854 1104 54-55 0.06089354002492087 1104 56-57 0.10058475522790644 1104 58-59 -0.02127718240851806 1104 60-61 0.12630811301163192 1104 62-63 0.11674593954485601 1104 64-65 0.16183358938029002 1104 66-67 0.18953254570019595 1104 68-69 0.09389608316218201 1104 70-71 0.10732729271606445 1104 72-73 0.05795942053914516 1104 74-75 0.07158301259430999 1104 76-77 0.04585229405982005 1104 78-79 0.008420072075580265 1104 80-81 0.05744752134560471 1104 82-83 0.10571915909612173 1104 84-85 -0.0017833752753979581 1104 86-87 5.565512366771941E-4 1104 88-89 0.01903533298924387 1104 90-91 0.0225638000736339 1104 92-93 0.07841484033122015 1104 94-95 0.022030968703077747 1104 96-97 0.023181190001739793 1104 98-99 0.020982756564499994 1104 100 0.02689339252251699 1105 1 0.12599859917752454 1105 2 0.15514997563997923 1105 3 0.10382254477162434 1105 4 0.04818579362623865 1105 5 0.03165500853431524 1105 6 0.1275285473814236 1105 7 0.019068438365465568 1105 8 0.0403799144032746 1105 9 0.16467971266546755 1105 10-11 0.1405194245073531 1105 12-13 0.17725748009270603 1105 14-15 0.2828694222165069 1105 16-17 0.2165219446164528 1105 18-19 0.20389530737976713 1105 20-21 0.0159976529060728 1105 22-23 0.09253451042003746 1105 24-25 0.07194089224677214 1105 26-27 0.18280226783374331 1105 28-29 0.20813664391435083 1105 30-31 0.135056318334712 1105 32-33 0.17944750958928068 1105 34-35 0.13799154795683677 1105 36-37 0.13339050206921144 1105 38-39 0.1600032928190558 1105 40-41 0.08865802830583647 1105 42-43 0.09082664195949519 1105 44-45 0.1541447388490056 1105 46-47 0.21100793270554163 1105 48-49 0.03880743829881084 1105 50-51 -0.03125012444487396 1105 52-53 -0.03505223263866952 1105 54-55 -0.21545736900077372 1105 56-57 -0.17170162116458698 1105 58-59 -0.20506427694362372 1105 60-61 -0.12899405903033312 1105 62-63 -0.16628322280335794 1105 64-65 -0.112871511630658 1105 66-67 -0.1661714508385046 1105 68-69 -0.10262103403918132 1105 70-71 -0.12117612442518677 1105 72-73 -0.15074046227391946 1105 74-75 -0.1094007100735297 1105 76-77 -0.23659546815156673 1105 78-79 -0.20662341803969042 1105 80-81 -0.1906368439412809 1105 82-83 -0.14082461791207024 1105 84-85 -0.18683428494426835 1105 86-87 -0.1326181785146474 1105 88-89 -0.23687571444880007 1105 90-91 -0.21001168955173455 1105 92-93 -0.29169088824374256 1105 94-95 -0.3773050390589745 1105 96-97 -0.4381510132174782 1105 98-99 -0.35991811579688715 1105 100 -0.39029002085701947 1106 1 0.04714952609577594 1106 2 0.03216046372734738 1106 3 5.473808413682946E-4 1106 4 6.670849228029851E-4 1106 5 0.014771594999409388 1106 6 -0.03901065758142863 1106 7 -0.143932979698981 1106 8 -0.07480651831260587 1106 9 -0.09011015442366244 1106 10-11 -0.027900397364213347 1106 12-13 -0.09317878399650681 1106 14-15 0.012861049444708783 1106 16-17 0.05724921658941895 1106 18-19 -0.015072620045074814 1106 20-21 -0.19971139065534516 1106 22-23 -0.17492117464113477 1106 24-25 -0.1284228940212202 1106 26-27 -0.03722924557971652 1106 28-29 -0.05971818660621153 1106 30-31 -0.06291333324341508 1106 32-33 -0.09683041250163882 1106 34-35 -0.056558252420735755 1106 36-37 -0.04928234423177713 1106 38-39 -0.04540081789072303 1106 40-41 -0.14155331247332725 1106 42-43 -0.1643330265313665 1106 44-45 -0.1681925000865192 1106 46-47 -0.02282617374483209 1106 48-49 0.006289378728013162 1106 50-51 -0.08998486488396651 1106 52-53 -0.150993177960423 1106 54-55 -0.2288358213026953 1106 56-57 -0.26848711983868867 1106 58-59 -0.2807788142152887 1106 60-61 -0.3152230298449936 1106 62-63 -0.2290926816267671 1106 64-65 -0.2934514167433768 1106 66-67 -0.2252936632374869 1106 68-69 -0.3131911900906559 1106 70-71 -0.405490819489863 1106 72-73 -0.48900543062350366 1106 74-75 -0.45382240256260076 1106 76-77 -0.4558517041167143 1106 78-79 -0.4536623827092079 1106 80-81 -0.3901806341127205 1106 82-83 -0.4026181306749024 1106 84-85 -0.48410499795098616 1106 86-87 -0.43521921796780205 1106 88-89 -0.4026792027538342 1106 90-91 -0.346674826539477 1106 92-93 -0.48760802229473654 1106 94-95 -0.49690607297339895 1106 96-97 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2216 4 -0.42567292163094095 2216 5 -0.42539578448972293 2216 6 -0.22687862466649733 2216 7 -0.2803631222980272 2216 8 -0.35266286014284276 2216 9 -0.17156795679829884 2216 10-11 -0.38317947939813735 2216 12-13 -0.3657854605448776 2216 14-15 -0.14635984902825783 2216 16-17 -0.4735940218399506 2216 18-19 -0.3973219131043848 2216 20-21 -0.3394791226401779 2216 22-23 -0.537308161005349 2216 24-25 -0.4653064666607918 2216 26-27 -0.42843269098684544 2216 28-29 -0.3371431724716487 2216 30-31 -0.34347355335510343 2216 32-33 -0.4714713374460331 2216 34-35 -0.8801312542204016 2216 36-37 -0.7551065092948548 2216 38-39 -0.5792022705528694 2216 40-41 -0.7923190559730884 2216 42-43 -0.5776467072939937 2216 44-45 -0.8100655860052299 2216 46-47 -0.4206752760905488 2216 48-49 -0.36750650677100083 2216 50-51 -0.4069994646842616 2216 52-53 -0.49795742700180057 2216 54-55 -0.5171146830917479 2216 56-57 -0.42371851166795693 2216 58-59 -0.5116628098704652 2216 60-61 -0.35942481504454804 2216 62-63 -0.3788408829662302 2216 64-65 -0.6705745795305482 2216 66-67 -0.6287707929908564 2216 68-69 -0.5401831327932669 2216 70-71 -0.6049120716061509 2216 72-73 -0.6192248804203153 2216 74-75 -0.6874711077213576 2216 76-77 -0.6182812612379784 2216 78-79 -0.5014825665516867 2216 80-81 -0.5375778212360167 2216 82-83 -0.45871866916381165 2216 84-85 -0.6201645473276685 2216 86-87 -0.5369361385378966 2216 88-89 -0.4842716244034406 2216 90-91 -0.4815502533539373 2216 92-93 -0.6112013625640387 2216 94-95 -0.5225078424320664 2216 96-97 -0.6054269010640638 2216 98-99 -0.5200641592112447 2216 100 -0.4807978749043862 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 360373.0 3 58863.0 4 38902.0 5 30826.0 6 28816.0 7 30846.0 8 34015.0 9 37680.0 10 41196.0 11 44109.0 12 46487.0 13 47450.0 14 48631.0 15 49314.0 16 50780.0 17 50647.0 18 50814.0 19 50949.0 20 52544.0 21 55875.0 22 64010.0 23 78608.0 24 97447.0 25 120809.0 26 145916.0 27 169240.0 28 203510.0 29 242981.0 30 288118.0 31 344509.0 32 424124.0 33 533584.0 34 691260.0 35 929559.0 36 1306161.0 37 1630065.0 38 1253212.0 39 240533.0 40 222.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.70278729662992 18.981504910442464 13.446262264056974 23.86944552887064 2 16.712550221855878 19.945628639854363 38.05485058169381 25.286970556595946 3 18.556817497138038 25.40005221827238 29.31728625655239 26.725844028037194 4 13.47607066809633 17.128088510564453 36.53070305860122 32.865137762737994 5 14.240119365772912 36.23869257679034 33.32642001208643 16.194768045350315 6 31.37597914214692 35.855738268310795 18.350008601776683 14.418273987765604 7 28.17271092201218 31.18779154392036 21.95208360033677 18.687413933730692 8 26.28995043110586 34.067037939925186 20.336494408319624 19.306517220649326 9 25.873473048609842 17.157564914533676 20.613942871778086 36.3550191650784 10-11 24.986988159764774 25.978171016699765 27.63857867001506 21.3962621535204 12-13 25.196568748311023 23.767169880655388 27.547360888631733 23.48890048240186 14-15 22.968870437072603 25.499572005072825 24.882181608625746 26.649375949228826 16-17 23.042889640602233 28.33624533333226 25.214952965287708 23.405912060777794 18-19 23.130669885848665 27.639952254332513 26.562763659447835 22.66661420037099 20-21 24.201043507160215 27.101734923974657 25.885609370772972 22.811612198092163 22-23 23.808688333230815 26.99623442210014 25.91449498138684 23.2805822632822 24-25 23.506853873094663 27.02287203168537 26.05406968579686 23.41620440942311 26-27 23.459109850899296 26.947949636413103 26.07428349618372 23.518657016503884 28-29 23.288737166845706 27.024341728594454 26.25422704671741 23.432694057842436 30-31 23.571798846505317 27.261561669009353 25.880022702557852 23.28661678192748 32-33 23.294490113486543 27.009881695858635 26.18605241225147 23.509575778403352 34-35 23.517759086845107 26.656859470779672 26.293991216168045 23.531390226207176 36-37 23.751735753321206 26.96812819112344 26.01591698217985 23.2642190733755 38-39 23.540949136410095 27.020443375690768 26.039390514074206 23.399216973824927 40-41 23.455149318315797 26.821879115503773 26.181464711710923 23.541506854469514 42-43 23.273833700914935 26.924956403258133 26.308081635479308 23.493128260347625 44-45 23.3856868145103 26.968685264882055 26.090325060620174 23.555302859987474 46-47 23.33838113863516 26.895423128073197 26.243326024368148 23.522869708923498 48-49 23.297621453539 26.83728901455803 26.309633497493053 23.55545603440992 50-51 23.203030385279703 26.845707856421942 26.202891864218053 23.748369894080305 52-53 23.211328378605018 26.719898314739183 26.267775969542583 23.80099733711322 54-55 23.231969936880713 27.018058570367405 26.18894789667679 23.561023596075096 56-57 22.949444184271407 26.82963671622106 26.27695895038031 23.94396014912723 58-59 23.003860421230993 26.50213388706572 26.36971409445049 24.124291597252796 60-61 23.242633621870954 26.80060262351226 26.206552615151963 23.75021113946482 62-63 23.014984161372492 26.909397190997765 26.16736820316387 23.90825044446587 64-65 23.120565376300306 26.47858081205418 26.31539579040036 24.085458021245156 66-67 22.905277458580194 26.622919665807025 26.28059436722246 24.191208508390318 68-69 23.208463163932183 26.623648851164138 26.155746948761426 24.01214103614225 70-71 23.14697509980583 26.383217157646975 26.352140644451826 24.117667098095367 72-73 22.901551294866444 26.53206035147056 26.495598314086106 24.070790039576888 74-75 22.94340518489766 26.361776506963135 26.48266878701 24.21214952112921 76-77 23.009208196446206 26.371859225893424 26.30263156904567 24.3163010086147 78-79 22.927013548452315 26.522863248452843 26.483023860236543 24.0670993428583 80-81 22.78804624772433 26.3488037432776 26.63103260171269 24.23211740728538 82-83 22.80710582529854 26.354454631500502 26.58153869710549 24.256900846095473 84-85 22.833781677930094 26.34831365689299 26.470076353288018 24.3478283118889 86-87 22.9568807023249 26.30111752160475 26.49211456772514 24.249887208345214 88-89 22.80994171791237 26.17407748958087 26.631879891561024 24.384100900945736 90-91 22.785132117214893 26.065595418523703 26.652568972603618 24.496703491657783 92-93 22.929095920738003 26.182800753624313 26.508660890030832 24.37944243560685 94-95 22.93157146700888 25.942634422086037 26.629096631946798 24.49669747895829 96-97 22.790676813132595 25.975944804434764 26.665939539620215 24.567438842812425 98-99 22.766997017422 26.035381580255653 26.745423166986743 24.452198235335608 100 22.887576047141902 25.89190720753731 26.594326147775565 24.62619059754522 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9383.0 1 6359.5 2 4025.0 3 4623.0 4 4114.5 5 3437.0 6 2955.5 7 2798.0 8 2784.5 9 2678.0 10 2523.5 11 2389.5 12 2332.0 13 2340.5 14 2424.0 15 2511.0 16 2778.5 17 3171.5 18 3675.0 19 4567.5 20 5962.0 21 7840.5 22 10589.0 23 14660.0 24 20370.5 25 28037.0 26 38321.0 27 51210.5 28 66340.0 29 84364.0 30 102484.5 31 118382.5 32 133186.5 33 148357.0 34 165494.0 35 184388.0 36 204823.0 37 228163.0 38 250926.5 39 269406.0 40 288076.5 41 306596.0 42 318376.0 43 327928.5 44 335506.5 45 340678.0 46 347114.0 47 345702.5 48 341819.0 49 347346.0 50 354075.0 51 355367.5 52 351770.0 53 348908.0 54 343776.0 55 331281.0 56 311909.0 57 287298.5 58 265464.0 59 247553.5 60 225899.0 61 197159.0 62 169322.5 63 142926.0 64 117023.5 65 96689.5 66 78216.0 67 60171.5 68 45970.0 69 35391.5 70 27055.5 71 20765.5 72 16002.0 73 11919.0 74 9361.0 75 6898.0 76 4522.0 77 3269.5 78 2516.0 79 1800.0 80 1141.5 81 581.0 82 325.0 83 211.5 84 154.5 85 120.5 86 100.0 87 86.5 88 63.5 89 49.5 90 40.0 91 29.5 92 20.5 93 19.5 94 19.5 95 14.0 96 12.0 97 14.5 98 14.5 99 18.0 100 27.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13838384395444295 2 0.15210090058292478 3 0.14825049872229829 4 0.1543569954231356 5 0.16406321678013153 6 0.0995389043501018 7 0.09030395613750547 8 0.09099582522183679 9 0.08791750915097135 10-11 0.08044231491373947 12-13 0.07662700786173848 14-15 0.07349354280589011 16-17 0.07919394243548947 18-19 0.11063889096393907 20-21 0.07380939608351962 22-23 0.07203460147588711 24-25 0.07142796264107486 26-27 0.06954287006347648 28-29 0.06825438923251163 30-31 0.09375327447098336 32-33 0.09538768984411387 34-35 0.06852010706924758 36-37 0.11202262913260173 38-39 0.06669517702072147 40-41 0.0687858249059835 42-43 0.08099380476356878 44-45 0.0933020555029412 46-47 0.07618581598187504 48-49 0.07507280919403769 50-51 0.07010438700148451 52-53 0.06563230567377772 54-55 0.06238352910387412 56-57 0.06344640045081788 58-59 0.0621378654434956 60-61 0.06248379998566126 62-63 0.0654568316306502 64-65 0.06401293093291527 66-67 0.06605344337728374 68-69 0.07133270530337708 70-71 0.07279164663338007 72-73 0.078471992086622 74-75 0.06692580004883192 76-77 0.0661035788181773 78-79 0.06735696484051665 80-81 0.06978352017976563 82-83 0.0687056082005538 84-85 0.06897132603728974 86-87 0.06618379552360702 88-89 0.06475493545814016 90-91 0.07308744573465216 92-93 0.06764273685361002 94-95 0.06905154274271946 96-97 0.06840479555519235 98-99 0.06120534624287513 100 0.06157634850548758 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9972985.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.27695393764568 #Duplication Level Percentage of deduplicated Percentage of total 1 79.55115775720509 44.768968407886156 2 11.385304283044867 12.814604894059919 3 3.5697435614438264 6.026828819297443 4 1.6630802559341968 3.743723638312671 5 0.9160279102977736 2.5775630256712816 6 0.5799944396513763 1.9584192218610672 7 0.3882982185576786 1.5296568671888247 8 0.2883700654217444 1.2982871110988305 9 0.2084239780581503 1.0556519951411456 >10 1.2823453750046918 13.363340870827326 >50 0.10878437839736829 4.237431918831146 >100 0.05608611936511843 5.5055879351648755 >500 0.0020435172067832406 0.7691524738827157 >1k 3.043363023081006E-4 0.21938817902931274 >5k 3.580410913720077E-5 0.13139464174717008 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.0027088178714799E-5 0.0 2 0.0 0.0 0.0 1.0027088178714799E-5 0.0 3 0.0 0.0 0.0 1.0027088178714799E-5 0.0 4 0.0 0.0 0.0 1.0027088178714799E-5 0.0 5 0.0 0.0 0.0 1.0027088178714799E-5 0.0 6 0.0 0.0 0.0 1.0027088178714799E-5 0.0 7 0.0 0.0 0.0 1.0027088178714799E-5 0.0 8 0.0 0.0 0.0 1.0027088178714799E-5 0.0 9 0.0 0.0 0.0 1.0027088178714799E-5 0.0 10-11 0.0 0.0 0.0 2.0054176357429597E-5 5.013544089357399E-6 12-13 0.0 0.0 0.0 5.013544089357399E-5 1.0027088178714799E-5 14-15 0.0 0.0 0.0 6.0162529072288785E-5 1.0027088178714799E-5 16-17 0.0 0.0 0.0 9.525733769779059E-5 1.0027088178714799E-5 18-19 0.0 5.013544089357399E-6 0.0 1.7046049903815156E-4 1.0027088178714799E-5 20-21 0.0 1.0027088178714799E-5 0.0 3.108397335401588E-4 1.0027088178714799E-5 22-23 0.0 1.5040632268072198E-5 0.0 8.021670542971838E-4 1.0027088178714799E-5 24-25 0.0 2.5067720446786995E-5 0.0 0.001960295738938743 1.0027088178714799E-5 26-27 0.0 3.509480862550179E-5 0.0 0.004045930080111421 1.0027088178714799E-5 28-29 0.0 5.013544089357399E-5 0.0 0.010027088178714798 1.0027088178714799E-5 30-31 0.0 5.013544089357399E-5 0.0 0.026371241910019917 1.0027088178714799E-5 32-33 0.0 6.0162529072288785E-5 0.0 0.06368705056710704 1.0027088178714799E-5 34-35 0.0 6.0162529072288785E-5 0.0 0.11879091365323421 1.0027088178714799E-5 36-37 0.0 8.523024951907578E-5 0.0 0.2017700818761885 1.0027088178714799E-5 38-39 0.0 1.1531151405522018E-4 0.0 0.3237796908347902 1.0027088178714799E-5 40-41 0.0 1.2032505814457757E-4 0.0 0.47173940399990577 1.0027088178714799E-5 42-43 0.0 1.2032505814457757E-4 0.0 0.624928243650221 1.0027088178714799E-5 44-45 0.0 1.2032505814457757E-4 0.0 0.8059322259082913 1.0027088178714799E-5 46-47 0.0 1.2032505814457757E-4 0.0 1.0189677413532658 1.0027088178714799E-5 48-49 0.0 1.25338602233935E-4 0.0 1.2520524196115805 1.0027088178714799E-5 50-51 0.0 1.3035214632329238E-4 0.0 1.4925320754016977 1.0027088178714799E-5 52-53 0.0 1.3536569041264977E-4 0.0 1.73788489604667 1.0027088178714799E-5 54-55 0.0 1.5040632268072197E-4 0.0 2.0037782068257397 1.0027088178714799E-5 56-57 0.0 1.6043341085943678E-4 0.0 2.303863888294227 1.0027088178714799E-5 58-59 0.0 1.7046049903815156E-4 0.0 2.6227353194655363 1.0027088178714799E-5 60-61 0.0 1.7046049903815156E-4 0.0 2.957464590591483 1.5040632268072198E-5 62-63 0.0 1.7046049903815156E-4 0.0 3.298104830198782 2.0054176357429597E-5 64-65 0.0 1.8048758721686634E-4 0.0 3.6627047970091198 2.0054176357429597E-5 66-67 0.0 1.9051467539558115E-4 0.0 4.0587246446274605 2.0054176357429597E-5 68-69 5.013544089357399E-6 1.9051467539558115E-4 0.0 4.477651375190076 2.0054176357429597E-5 70-71 1.0027088178714799E-5 1.9552821948493857E-4 0.0 4.921961679477107 2.0054176357429597E-5 72-73 1.0027088178714799E-5 2.1056885175301077E-4 0.0 5.385945130770777 2.0054176357429597E-5 74-75 1.0027088178714799E-5 2.1056885175301077E-4 0.0 5.84928183487692 2.0054176357429597E-5 76-77 1.0027088178714799E-5 2.1056885175301077E-4 0.0 6.339185309112567 2.0054176357429597E-5 78-79 1.0027088178714799E-5 2.1056885175301077E-4 0.0 6.855119104260159 2.0054176357429597E-5 80-81 1.0027088178714799E-5 2.1558239584236816E-4 0.0 7.400081319685129 2.0054176357429597E-5 82-83 1.0027088178714799E-5 2.2059593993172555E-4 0.0 7.964245408972339 2.0054176357429597E-5 84-85 1.0027088178714799E-5 2.2059593993172555E-4 0.0 8.514998267820516 2.0054176357429597E-5 86-87 1.0027088178714799E-5 2.2059593993172555E-4 0.0 9.08147861447701 2.0054176357429597E-5 88 1.0027088178714799E-5 2.3062302811044036E-4 0.0 9.531068180690134 2.0054176357429597E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 1485 0.0 42.120213 2 TAACGCA 1580 0.0 38.09922 4 TACCTGG 10810 0.0 37.196815 2 ACCTGGG 12755 0.0 30.934767 3 GTACCTG 13695 0.0 30.219412 1 GGTATCA 9045 0.0 29.792807 1 GTATCAA 13025 0.0 29.426949 1 GTATAAC 3290 0.0 26.158966 1 TCAACGC 13825 0.0 25.989109 4 ATAACGC 2640 0.0 25.8303 3 GTACATG 30835 0.0 25.760307 1 ATCAACG 14415 0.0 24.92551 3 CAACGCA 14950 0.0 24.253609 5 TACATGG 31945 0.0 24.040787 2 TATCACG 1865 0.0 23.95577 2 ACATGGG 32270 0.0 22.938824 3 AACGCAG 16155 0.0 22.633875 6 TATCAAC 16945 0.0 21.759047 2 CCTGGGG 13840 0.0 20.99982 4 ACGCAGA 18390 0.0 19.75393 7 >>END_MODULE