FastQCFastQC Report
Wed 25 May 2016
SRR2049339_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049339_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9972985
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216770.21735719045000065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197310.19784447685422166No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169320.16977865704199896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126390.12673236749077632No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG16600.053.797452
GGTATCA159700.047.8708571
TAACGCA19550.045.4392554
GTATCAA266300.036.953341
TACCTGG108650.036.4249152
TATCACG18550.033.1927762
GTATAAC34750.032.7428251
ATAACGC29050.032.5211683
ACCTGGG126300.030.1810073
TCAACGC326450.029.8036794
ATCAACG328950.029.5057323
GTACCTG139000.029.0558761
CAACGCA341250.028.497325
TATCAAC347700.027.901092
AACGCAG361050.027.2982146
ACGCAGA413100.023.8458637
CGCAGAG422900.023.2821668
GTATCAC34000.021.2959691
GCAGAGT466300.021.0043549
GTGGTAT85900.020.7444361
Produced by FastQC (version 0.11.5)