##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049339_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9972985
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.247212444418597	31.0	31.0	34.0	28.0	34.0
2	31.261697876814214	31.0	31.0	34.0	28.0	34.0
3	31.404769183950442	31.0	31.0	34.0	28.0	34.0
4	35.11159828276088	37.0	35.0	37.0	32.0	37.0
5	35.25528585473657	37.0	35.0	37.0	33.0	37.0
6	35.325635905398435	37.0	35.0	37.0	33.0	37.0
7	35.26615832671963	37.0	35.0	37.0	32.0	37.0
8	35.2339838072553	37.0	35.0	37.0	32.0	37.0
9	36.83418986391737	39.0	37.0	39.0	33.0	39.0
10-11	36.710604096967955	39.0	36.5	39.0	32.0	39.0
12-13	36.67193533330292	39.0	35.0	39.0	32.0	39.0
14-15	37.80667232528676	39.0	37.0	41.0	33.0	41.0
16-17	37.81070637326738	40.0	37.0	41.0	33.0	41.0
18-19	37.841642747883405	39.5	37.0	41.0	32.5	41.0
20-21	37.901565128193816	40.0	37.0	41.0	33.0	41.0
22-23	37.8172765225256	40.0	37.0	41.0	32.5	41.0
24-25	37.666452772164	39.0	37.0	41.0	32.0	41.0
26-27	37.629334096060504	39.0	37.0	41.0	32.0	41.0
28-29	37.57495895160777	39.0	37.0	41.0	32.0	41.0
30-31	37.4687744441609	39.0	36.5	41.0	32.0	41.0
32-33	37.3410619789361	39.0	36.0	41.0	31.5	41.0
34-35	37.230727610640145	39.0	36.0	41.0	31.0	41.0
36-37	37.10424652197912	39.0	36.0	41.0	31.0	41.0
38-39	37.00234052292268	39.0	36.0	41.0	31.0	41.0
40-41	36.88672664202343	39.0	35.0	41.0	31.0	41.0
42-43	36.701804424653204	39.0	35.0	41.0	30.0	41.0
44-45	36.567373008181605	39.0	35.0	40.5	30.0	41.0
46-47	36.362337103685604	39.0	35.0	40.0	30.0	41.0
48-49	36.1186245141249	39.0	35.0	40.0	30.0	41.0
50-51	35.9409746931335	39.0	35.0	40.0	29.0	41.0
52-53	35.80409295712367	38.0	35.0	40.0	29.0	41.0
54-55	35.63435225261043	38.0	34.5	40.0	28.0	41.0
56-57	35.462228359914306	38.0	34.0	40.0	28.0	41.0
58-59	35.23672029989015	38.0	34.0	40.0	28.0	41.0
60-61	35.017273113315625	37.0	34.0	40.0	28.0	41.0
62-63	34.7614681060886	37.0	34.0	40.0	28.0	41.0
64-65	34.51168887750257	36.0	34.0	39.5	27.0	41.0
66-67	34.1888334836561	36.0	33.0	39.0	27.0	41.0
68-69	33.91501205506676	35.0	33.0	39.0	26.0	40.5
70-71	33.647018721074986	35.0	33.0	38.5	26.0	40.0
72-73	33.33582688633343	35.0	33.0	37.5	26.0	40.0
74-75	33.048875336722155	35.0	33.0	37.0	26.0	39.0
76-77	32.7632177327049	35.0	32.5	36.5	26.0	39.0
78-79	32.50235471125245	35.0	32.0	36.0	26.0	39.0
80-81	32.194710259766765	35.0	32.0	36.0	25.0	37.5
82-83	31.98753517627872	35.0	32.0	35.0	25.0	37.0
84-85	31.704000206558018	35.0	32.0	35.0	25.0	37.0
86-87	31.546713596781707	35.0	32.0	35.0	25.0	36.0
88-89	31.387277730789727	34.0	32.0	35.0	24.5	36.0
90-91	31.221985192998886	34.0	32.0	35.0	24.0	36.0
92-93	30.967905897782863	34.0	32.0	35.0	23.5	35.0
94-95	30.82882471998103	34.0	31.0	35.0	23.0	35.0
96-97	30.672834813247988	34.0	31.5	35.0	21.5	35.0
98-99	30.468942598429656	34.0	31.0	35.0	20.0	35.0
100	30.336726767362027	34.0	31.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.1824691632397233
1101	2	0.20986285556648454
1101	3	0.2513396398497001
1101	4	0.34114468523141284
1101	5	0.2807546668257004
1101	6	0.25059330930592694
1101	7	0.1678101617088359
1101	8	0.23323014074495774
1101	9	0.2787036336329223
1101	10-11	0.37711480575946155
1101	12-13	0.39798775218778104
1101	14-15	0.42148044093040227
1101	16-17	0.4610674550826843
1101	18-19	0.5006039846113168
1101	20-21	0.41985390919949594
1101	22-23	0.3936268954833082
1101	24-25	0.5043598777094545
1101	26-27	0.42131276444191457
1101	28-29	0.310341224383599
1101	30-31	0.2836062514564617
1101	32-33	0.2866518478383355
1101	34-35	0.3751436031410904
1101	36-37	0.43391818820466455
1101	38-39	0.4506000387367166
1101	40-41	0.4372124056353641
1101	42-43	0.4138574001361164
1101	44-45	0.3622979331966789
1101	46-47	0.4112565375523758
1101	48-49	0.5256337307243157
1101	50-51	0.5785501266570776
1101	52-53	0.572287438349214
1101	54-55	0.45992130356898997
1101	56-57	0.43566546711978305
1101	58-59	0.519314565337126
1101	60-61	0.4446452677556749
1101	62-63	0.4353312847356321
1101	64-65	0.42697443555708503
1101	66-67	0.4785919604260016
1101	68-69	0.43014286288093473
1101	70-71	0.4532766851437344
1101	72-73	0.3876364106071435
1101	74-75	0.3978170131266907
1101	76-77	0.39319556319772886
1101	78-79	0.4287633299992706
1101	80-81	0.4488968366862025
1101	82-83	0.352842681805825
1101	84-85	0.4273016097496125
1101	86-87	0.41328730336948283
1101	88-89	0.3385644263248331
1101	90-91	0.42727031619617506
1101	92-93	0.5173762912286115
1101	94-95	0.47475278207761207
1101	96-97	0.4530729213793592
1101	98-99	0.41282490817097184
1101	100	0.39150976228757983
1102	1	0.192172606250697
1102	2	0.22961437067045054
1102	3	0.2023507969948959
1102	4	0.27996355683772833
1102	5	0.22616137218366106
1102	6	0.2869091947261708
1102	7	0.25958427877081647
1102	8	0.25367794225604
1102	9	0.26533801287779113
1102	10-11	0.31705251645692556
1102	12-13	0.27887229853048723
1102	14-15	0.45303076892597716
1102	16-17	0.42109042708587197
1102	18-19	0.4508877322187175
1102	20-21	0.38369795717112964
1102	22-23	0.33494705886980114
1102	24-25	0.5452702182490796
1102	26-27	0.32533129045480536
1102	28-29	0.40785294847373876
1102	30-31	0.4273391344156181
1102	32-33	0.4316697415272799
1102	34-35	0.4350602607091858
1102	36-37	0.4302455770609015
1102	38-39	0.3979379037068398
1102	40-41	0.4167354559843943
1102	42-43	0.44221395009908093
1102	44-45	0.4030110198288739
1102	46-47	0.4574850515595017
1102	48-49	0.5189327095696328
1102	50-51	0.43352070991844016
1102	52-53	0.4095236803727076
1102	54-55	0.43462543088022443
1102	56-57	0.41457898082943956
1102	58-59	0.4779331009607404
1102	60-61	0.46972921712267635
1102	62-63	0.45496383729109624
1102	64-65	0.456603083896276
1102	66-67	0.5417019874079188
1102	68-69	0.4981424086058084
1102	70-71	0.4923876047670035
1102	72-73	0.4772177158292479
1102	74-75	0.44697943724073497
1102	76-77	0.45926431706735116
1102	78-79	0.3968288322440685
1102	80-81	0.4822951158889879
1102	82-83	0.36718811592133704
1102	84-85	0.4893622900748227
1102	86-87	0.4088913280138833
1102	88-89	0.3589557703406605
1102	90-91	0.38823184468322935
1102	92-93	0.5509310169429789
1102	94-95	0.4295592313460084
1102	96-97	0.43992941217432247
1102	98-99	0.3819665216027843
1102	100	0.35609158509347694
1103	1	0.06585912859393517
1103	2	0.08507400941367038
1103	3	0.05933076203456267
1103	4	0.19697848221085934
1103	5	0.16071293867552328
1103	6	0.1537425952909146
1103	7	0.15998049588345253
1103	8	0.16206931870711827
1103	9	0.1334685314560673
1103	10-11	0.2597588048348527
1103	12-13	0.26976109777750423
1103	14-15	0.2882824385060019
1103	16-17	0.3267284531716541
1103	18-19	0.25245780975895116
1103	20-21	0.2857283457686748
1103	22-23	0.22197139664041288
1103	24-25	0.3945831132336153
1103	26-27	0.20273615800893197
1103	28-29	0.16805464270528603
1103	30-31	0.2040663093517452
1103	32-33	0.2223254718845098
1103	34-35	0.2578718782969176
1103	36-37	0.20529689703759146
1103	38-39	0.20520158529888732
1103	40-41	0.19060574418303844
1103	42-43	0.2872208973448309
1103	44-45	0.19351884108296957
1103	46-47	0.2048406160792453
1103	48-49	0.26504359657604226
1103	50-51	0.3433898327726368
1103	52-53	0.298440358244612
1103	54-55	0.2510917479435619
1103	56-57	0.21987323030278816
1103	58-59	0.2102185207088425
1103	60-61	0.22419383099028067
1103	62-63	0.2447276309354791
1103	64-65	0.20834482563801515
1103	66-67	0.25580489628694636
1103	68-69	0.23610350402959313
1103	70-71	0.2705131930418432
1103	72-73	0.24888012450658437
1103	74-75	0.17541697926036903
1103	76-77	0.3184718828420827
1103	78-79	0.21592165641150984
1103	80-81	0.2044393496750132
1103	82-83	0.2510615415858055
1103	84-85	0.19324781739318553
1103	86-87	0.20872728337222313
1103	88-89	0.21353736686255687
1103	90-91	0.17155621382849162
1103	92-93	0.2612954111879695
1103	94-95	0.18649078977010447
1103	96-97	0.17750773676757348
1103	98-99	0.20958384041413325
1103	100	0.16808118663531602
1104	1	0.021106475367737687
1104	2	0.024388151742474662
1104	3	0.07763201206058312
1104	4	0.15121985380477554
1104	5	0.11002070163895183
1104	6	0.10549822613251791
1104	7	0.09993098749790619
1104	8	0.17920394716871613
1104	9	0.17400353513363598
1104	10-11	0.272996827153527
1104	12-13	0.18946032002400415
1104	14-15	0.18102188805046637
1104	16-17	0.20725705363170022
1104	18-19	0.22128059784085252
1104	20-21	0.16959298569665293
1104	22-23	0.154356281837849
1104	24-25	0.2830049163519206
1104	26-27	0.14158952454445028
1104	28-29	0.15365440930116847
1104	30-31	0.1704402581225395
1104	32-33	0.25770302536535183
1104	34-35	0.27569967836561204
1104	36-37	0.2651785968891005
1104	38-39	0.26911890377970593
1104	40-41	0.26004568273990714
1104	42-43	0.29073089269584074
1104	44-45	0.27458479357295573
1104	46-47	0.27993108077112794
1104	48-49	0.3621572004534457
1104	50-51	0.3427658042055697
1104	52-53	0.3096443127572641
1104	54-55	0.25122998848769384
1104	56-57	0.27168764584710203
1104	58-59	0.24236767512851287
1104	60-61	0.19633133688826376
1104	62-63	0.28336752942074384
1104	64-65	0.24091955441740964
1104	66-67	0.23102465046237342
1104	68-69	0.12062791605246304
1104	70-71	0.25435270364781104
1104	72-73	0.2688382488083647
1104	74-75	0.16430780234161801
1104	76-77	0.1732339598350805
1104	78-79	0.2017363243549255
1104	80-81	0.2740539488374978
1104	82-83	0.18101870095978967
1104	84-85	0.13102806712083748
1104	86-87	0.04825104921872381
1104	88-89	0.05966086591194042
1104	90-91	0.055811335101484616
1104	92-93	0.1432889672049562
1104	94-95	0.07346156481746391
1104	96-97	0.06389579516804034
1104	98-99	0.03633514534346105
1104	100	-9.807127242318359E-4
1105	1	-0.06879659273435479
1105	2	-0.013530709201592828
1105	3	-0.014722590177633776
1105	4	0.11503570730926072
1105	5	0.05431341181247262
1105	6	0.08629381537631531
1105	7	0.06825325143680772
1105	8	0.14036105994885872
1105	9	0.128088625079954
1105	10-11	0.14539086963933556
1105	12-13	0.08738539091264386
1105	14-15	0.17578302523337186
1105	16-17	0.11003578922222346
1105	18-19	0.1650859890524714
1105	20-21	0.17174662447081346
1105	22-23	0.12280035743860651
1105	24-25	0.32498929042884583
1105	26-27	0.21473837608117208
1105	28-29	0.19542221631488133
1105	30-31	0.1429988378084346
1105	32-33	0.14901877354589033
1105	34-35	0.12218004661014703
1105	36-37	0.2524441460226754
1105	38-39	0.2518544424264846
1105	40-41	0.28356673790011655
1105	42-43	0.34772364096293984
1105	44-45	0.313909282654798
1105	46-47	0.28967249157715713
1105	48-49	0.34281745096860305
1105	50-51	0.35830651101200317
1105	52-53	0.3411039418814994
1105	54-55	0.20160053525820842
1105	56-57	0.2992093948182273
1105	58-59	0.25748444238551826
1105	60-61	0.2649386267589833
1105	62-63	0.23301649438064231
1105	64-65	0.1671117496878125
1105	66-67	0.27650904034640433
1105	68-69	0.22984450686887214
1105	70-71	0.22357758026054597
1105	72-73	0.26815400042596593
1105	74-75	0.28166151298449194
1105	76-77	0.29517399269751365
1105	78-79	0.224215433799543
1105	80-81	0.2767490743938623
1105	82-83	0.20642319386186614
1105	84-85	0.2860607842460823
1105	86-87	0.1861886783298985
1105	88-89	0.18172645590236058
1105	90-91	0.14497511709988942
1105	92-93	0.19676599890913593
1105	94-95	0.0957414329042301
1105	96-97	0.12688249094903625
1105	98-99	0.10406084053940745
1105	100	0.04341393793509951
1106	1	-0.1806948642348729
1106	2	-0.06592715470642219
1106	3	-0.08521087267172689
1106	4	0.10329974974052902
1106	5	0.019539672787225015
1106	6	-0.050920207501789605
1106	7	0.01098761606883869
1106	8	0.032159369368770285
1106	9	-0.06666440177885136
1106	10-11	0.12665383779449257
1106	12-13	0.16498078609429712
1106	14-15	0.09345344394137811
1106	16-17	0.04769756751790766
1106	18-19	0.1265521329925292
1106	20-21	0.11386154050008912
1106	22-23	0.039079026030989894
1106	24-25	0.26131793571490647
1106	26-27	0.06282600574755293
1106	28-29	0.06346352329735794
1106	30-31	-0.015871846288462166
1106	32-33	0.004045677874422893
1106	34-35	-0.10295708752941124
1106	36-37	-0.039848831865782586
1106	38-39	0.04770217843743296
1106	40-41	0.01067549705678772
1106	42-43	0.11293173947871793
1106	44-45	0.040310113461949015
1106	46-47	-0.045717120624715335
1106	48-49	0.09068365038026371
1106	50-51	0.043583982951261646
1106	52-53	-0.06027491079239411
1106	54-55	-0.1289143397474959
1106	56-57	-0.13929506166770267
1106	58-59	-0.04840120996951214
1106	60-61	-0.04546031466699674
1106	62-63	-0.04101241237157893
1106	64-65	0.0017229621837131504
1106	66-67	0.016851812421712964
1106	68-69	0.022276832988566753
1106	70-71	0.025095648184610297
1106	72-73	-0.018693116723824232
1106	74-75	-0.0023760714922147486
1106	76-77	0.021798311307811957
1106	78-79	-0.06802332095269747
1106	80-81	-0.042869766933009146
1106	82-83	-0.09029297735113673
1106	84-85	-0.08927580147699032
1106	86-87	-0.16015226228200063
1106	88-89	-0.12812752998618038
1106	90-91	-0.07869318955036064
1106	92-93	0.008688066273546013
1106	94-95	-0.04734008890747532
1106	96-97	-0.18569129551989505
1106	98-99	-0.19017027609900694
1106	100	-0.17405183281447378
1107	1	-0.09892704804972752
1107	2	-0.05448069020650692
1107	3	-0.06529257290365109
1107	4	0.08742463852605908
1107	5	0.05116102084532059
1107	6	0.01702970811389548
1107	7	0.006546590143337028
1107	8	0.09906327366351775
1107	9	0.07610170965111251
1107	10-11	0.0822090268430884
1107	12-13	0.030306917068145367
1107	14-15	0.09310162215763285
1107	16-17	0.04580853662535844
1107	18-19	0.029104629424061557
1107	20-21	0.030243672361514484
1107	22-23	0.051738047258282904
1107	24-25	0.16186846068988103
1107	26-27	0.04930701340934718
1107	28-29	-0.020856767983211455
1107	30-31	-0.03668605784452694
1107	32-33	-0.03642700230368945
1107	34-35	0.06596046760842711
1107	36-37	-0.017168303839738996
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2216	96-97	-0.5729505395054701
2216	98-99	-0.5441416769472944
2216	100	-0.6357051891141658
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	156.0
3	57.0
4	151.0
5	316.0
6	963.0
7	2423.0
8	4965.0
9	7400.0
10	10904.0
11	15369.0
12	20251.0
13	24582.0
14	27183.0
15	33030.0
16	41661.0
17	52092.0
18	46874.0
19	31746.0
20	29156.0
21	30517.0
22	34842.0
23	40848.0
24	51395.0
25	66608.0
26	87984.0
27	116369.0
28	154627.0
29	202230.0
30	261488.0
31	331304.0
32	417920.0
33	533532.0
34	701648.0
35	961471.0
36	1420979.0
37	2008061.0
38	1786510.0
39	414806.0
40	567.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.071981993593646	19.27840971495373	13.729152634685704	23.92045565676692
2	16.86845011799376	20.044750894541604	38.12282882206281	24.963970165401832
3	18.37982299628453	25.59921900334286	29.791460817106525	26.229497183266087
4	13.438664552288007	17.257080001624388	36.63005609654482	32.67419934954279
5	14.188043152697485	35.635108680425795	33.73801563250958	16.438832534367144
6	30.926757733276332	36.17851558437951	18.20392586997071	14.690800812373443
7	27.648652835635467	31.239994846076673	22.279107007580983	18.832245310706874
8	26.320865819010052	33.729139269737196	20.43428321610832	19.515711695144432
9	25.727663282357287	16.405529538047034	21.022853238022517	36.84395394157316
10-11	25.0044996558202	25.51832776245026	27.846938504369557	21.63023407735999
12-13	25.62408358662599	23.302265731326326	28.04686421304438	23.02678646900331
14-15	23.11056628351311	24.854763843691078	25.767464824836985	26.267205047958825
16-17	22.603703425544794	27.657126258011267	26.23084286817695	23.508327448266982
18-19	22.591626278391075	26.381725230710767	28.614507090906084	22.412141399992077
20-21	23.616339541270744	25.74644903206011	28.253030562063415	22.38418086460573
22-23	23.22172849954151	25.70376873122741	28.248197505561272	22.826305263669806
24-25	23.062157418265443	25.758255928390543	28.32592749312267	22.853659160221337
26-27	23.044995168680558	25.68673647517582	28.310012831210347	22.958255524933275
28-29	22.894423627063954	25.694903323683917	28.46373246886334	22.94694058038879
30-31	23.137544626247518	26.005371218134055	28.107503175708782	22.74958097990965
32-33	22.976861992673207	25.707488780941713	28.31404539363089	23.001603832754185
34-35	23.114343398691563	25.427672858226497	28.371836516348914	23.08614722673302
36-37	23.36709119686834	25.79522078896138	28.024372843236	22.813315170934278
38-39	23.232673066288577	25.82409378937199	27.96936925103166	22.973863893307772
40-41	23.067238451400303	25.635597129204257	28.16484514564548	23.132319273749964
42-43	22.980995457327516	25.807536640989298	28.11212271071527	23.099345190967917
44-45	23.14128618462777	25.844137938641236	27.871038610807098	23.143537265923893
46-47	23.223869282867668	25.924264400277348	27.565438030840316	23.28642828601467
48-49	23.2465378466465	25.9011645862462	27.493035183852697	23.3592623832546
50-51	23.12219191367398	25.944261015559633	27.34059836053081	23.59294871023557
52-53	23.126140267933824	25.9029016889126	27.397634710169523	23.573323332984057
54-55	23.109916439260665	26.238177436344284	27.332238041067946	23.31966808332711
56-57	22.840954839498906	26.041756806011442	27.375540021367723	23.74174833312193
58-59	22.876210081535266	25.672083132582674	27.56028410751646	23.891422678365604
60-61	23.081349265039506	26.021436911817275	27.35516497818858	23.542048844954643
62-63	22.845141148813518	26.10649670083731	27.306197693067823	23.742164457281344
64-65	22.865471070095865	25.71992237028332	27.492962237484562	23.921644322136252
66-67	22.686089480051034	25.88525427126062	27.381355766038233	24.047300482650105
68-69	23.0355316405034	25.92090662529356	27.161468064048428	23.882093670154607
70-71	22.978842342588504	25.7750964229867	27.270546381048405	23.975514853376396
72-73	22.77942862643431	25.94907141643149	27.27970111255557	23.99179884457863
74-75	22.796334297103627	25.87048411283081	27.147814821740933	24.185366768324627
76-77	22.936011635433122	25.895195871647253	26.920946938153424	24.2478455547662
78-79	22.828610623042398	26.14112570754923	27.034624852890023	23.99563881651835
80-81	22.65235533794546	26.006301022211503	27.094941985774568	24.246401654068467
82-83	22.686982495400823	26.017958124648615	26.983988435150618	24.311070944799944
84-85	22.69344086919631	26.06142515233406	26.792510325145447	24.452623653324185
86-87	22.824182081192486	26.049441894470952	26.756482353142307	24.36989367119425
88-89	22.59403957612678	25.930198209522487	26.94191165691465	24.533850557436082
90-91	22.458539605232396	25.87864521758112	26.93390637493913	24.72890880224735
92-93	22.652147234434807	25.98841700216069	26.734262313484148	24.625173449920357
94-95	22.573339936510433	25.771296775275825	26.85678509034965	24.79857819786409
96-97	22.41272642808132	25.862352663131045	26.864244876079656	24.86067603270798
98-99	22.30830538740257	25.942390229759344	26.957778962423312	24.79152542041477
100	22.40307880107567	25.8285768947735	26.811693965279964	24.956650338870865
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1443.0
1	1447.0
2	7213.0
3	10507.0
4	5414.0
5	2474.5
6	2863.5
7	6063.0
8	9820.5
9	10815.0
10	8332.5
11	5456.5
12	4849.0
13	5071.5
14	5587.0
15	6371.5
16	7151.0
17	7913.5
18	9079.0
19	10283.5
20	11492.5
21	13268.5
22	15766.5
23	19478.0
24	24744.5
25	31754.5
26	41409.0
27	53464.5
28	67832.5
29	85187.0
30	102452.5
31	117856.5
32	132719.5
33	147001.5
34	162748.5
35	180879.0
36	200620.0
37	223472.5
38	246476.5
39	264899.0
40	283915.0
41	303360.5
42	314982.5
43	324568.0
44	333396.5
45	339638.0
46	346596.5
47	344915.5
48	340583.0
49	345627.5
50	352468.5
51	353946.0
52	349101.5
53	346069.0
54	341512.5
55	329159.5
56	310617.0
57	286206.0
58	264723.0
59	249215.5
60	227841.5
61	196255.5
62	167435.0
63	140997.0
64	114063.0
65	93066.5
66	74905.5
67	57271.5
68	43421.5
69	33084.0
70	24676.0
71	18524.5
72	13689.5
73	10146.5
74	8356.5
75	6236.5
76	3845.5
77	2700.5
78	2046.0
79	1371.5
80	787.5
81	341.5
82	160.5
83	80.5
84	47.5
85	28.0
86	15.0
87	13.0
88	10.0
89	4.0
90	1.0
91	1.0
92	1.5
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03208668217188736
2	0.0
3	8.723566715481874E-4
4	0.0
5	0.003068288982686728
6	0.007259611841389514
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	2.0054176357429597E-5
14-15	3.50948086255018E-5
16-17	2.0054176357429597E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.659887185230901E-4
28-29	1.5040632268072197E-4
30-31	1.4539277859136458E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	1.0027088178714799E-5
42-43	1.0027088178714799E-5
44-45	0.0
46-47	0.0
48-49	1.0027088178714799E-5
50-51	1.0027088178714799E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.02310742470784825
68-69	5.013544089357399E-6
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	4.0108352714859194E-5
80-81	0.0
82-83	6.016252907228879E-5
84-85	2.0054176357429597E-5
86-87	5.5148984982931394E-5
88-89	3.0081264536144396E-5
90-91	3.50948086255018E-5
92-93	5.5148984982931394E-5
94-95	8.021670542971839E-5
96-97	6.016252907228879E-5
98-99	6.517607316164619E-5
100	1.2032505814457758E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9972985.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.29864043762161
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3625463515143	35.43311247820137
2	14.654190747230736	14.155549796096375
3	4.744961534314671	6.8752557310862885
4	2.155137410133149	4.16360827462752
5	1.2166314890927195	2.9380823418388706
6	0.7768997175625153	2.2513920068785023
7	0.5140202165024716	1.73785343301649
8	0.3904541008862711	1.5086721780880672
9	0.28942839398183146	1.2581098140030085
>10	1.660075748274927	15.101203630354693
>50	0.14922996434357927	5.005275763657325
>100	0.08238755360766978	7.15052773764077
>500	0.0031427619101671305	1.0345344133088816
>1k	7.692653315491063E-4	0.5465216435226614
>5k	4.158177110556323E-5	0.12754986465319357
>10k+	8.316354221112646E-5	0.7127508930262183
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21677	0.21735719045000065	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19731	0.19784447685422166	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16932	0.16977865704199896	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12639	0.12673236749077632	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0027088178714799E-5	0.0
2	0.0	1.0027088178714799E-5	0.0	1.0027088178714799E-5	0.0
3	0.0	1.0027088178714799E-5	0.0	1.0027088178714799E-5	0.0
4	0.0	1.0027088178714799E-5	0.0	1.0027088178714799E-5	0.0
5	0.0	1.0027088178714799E-5	0.0	1.0027088178714799E-5	0.0
6	0.0	1.0027088178714799E-5	0.0	1.0027088178714799E-5	0.0
7	0.0	1.0027088178714799E-5	0.0	1.0027088178714799E-5	0.0
8	0.0	1.0027088178714799E-5	0.0	1.0027088178714799E-5	0.0
9	0.0	1.0027088178714799E-5	0.0	1.0027088178714799E-5	0.0
10-11	0.0	1.5040632268072198E-5	0.0	2.0054176357429597E-5	0.0
12-13	0.0	2.5067720446786995E-5	0.0	5.013544089357399E-5	0.0
14-15	0.0	4.0108352714859194E-5	0.0	7.018961725100358E-5	0.0
16-17	0.0	5.013544089357399E-5	0.0	9.024379360843318E-5	0.0
18-19	1.0027088178714799E-5	5.013544089357399E-5	0.0	1.6043341085943678E-4	0.0
20-21	1.0027088178714799E-5	5.013544089357399E-5	0.0	2.9078555718272916E-4	0.0
22-23	1.0027088178714799E-5	5.013544089357399E-5	0.0	7.921399661184689E-4	0.0
24-25	1.0027088178714799E-5	5.013544089357399E-5	0.0	0.001975336371206815	0.0
26-27	1.0027088178714799E-5	5.013544089357399E-5	0.0	0.004151214505987926	1.0027088178714799E-5
28-29	1.0027088178714799E-5	5.013544089357399E-5	0.0	0.01040811752950596	1.0027088178714799E-5
30-31	1.0027088178714799E-5	6.517607316164619E-5	0.0	0.02756947894737634	1.0027088178714799E-5
32-33	1.0027088178714799E-5	7.018961725100358E-5	0.0	0.06672024474116826	1.0027088178714799E-5
34-35	1.0027088178714799E-5	7.018961725100358E-5	0.0	0.12442112366558256	1.0027088178714799E-5
36-37	1.0027088178714799E-5	7.018961725100358E-5	0.0	0.21149635740954187	1.0027088178714799E-5
38-39	1.0027088178714799E-5	7.018961725100358E-5	2.0054176357429597E-5	0.33903590549870477	1.0027088178714799E-5
40-41	1.0027088178714799E-5	7.018961725100358E-5	2.0054176357429597E-5	0.4933076706723213	1.0027088178714799E-5
42-43	1.0027088178714799E-5	8.021670542971839E-5	2.0054176357429597E-5	0.6525528715825804	1.0027088178714799E-5
44-45	1.0027088178714799E-5	8.021670542971839E-5	2.0054176357429597E-5	0.8396182286446836	1.0027088178714799E-5
46-47	1.0027088178714799E-5	9.024379360843318E-5	2.0054176357429597E-5	1.0592565816553419	1.0027088178714799E-5
48-49	1.0027088178714799E-5	9.024379360843318E-5	2.0054176357429597E-5	1.2980717408077922	1.0027088178714799E-5
50-51	2.0054176357429597E-5	9.525733769779059E-5	2.0054176357429597E-5	1.5439158887735216	1.0027088178714799E-5
52-53	3.0081264536144392E-5	1.1029796996586277E-4	2.0054176357429597E-5	1.7946482422263745	1.0027088178714799E-5
54-55	3.0081264536144392E-5	1.1029796996586277E-4	2.0054176357429597E-5	2.0650236614213298	1.0027088178714799E-5
56-57	3.0081264536144392E-5	1.1029796996586277E-4	2.0054176357429597E-5	2.3706392820203783	1.0027088178714799E-5
58-59	3.0081264536144392E-5	1.1531151405522018E-4	2.0054176357429597E-5	2.694830083470496	1.0027088178714799E-5
60-61	3.509480862550179E-5	1.2032505814457757E-4	2.0054176357429597E-5	3.0359115149576583	1.0027088178714799E-5
62-63	4.0108352714859194E-5	1.2032505814457757E-4	2.0054176357429597E-5	3.3827685492357604	1.0027088178714799E-5
64-65	4.0108352714859194E-5	1.3035214632329238E-4	2.0054176357429597E-5	3.7540565838613014	1.0027088178714799E-5
66-67	4.0108352714859194E-5	1.3035214632329238E-4	2.0054176357429597E-5	4.156749458662577	1.0027088178714799E-5
68-69	4.0108352714859194E-5	1.3035214632329238E-4	2.0054176357429597E-5	4.582464527922182	1.0027088178714799E-5
70-71	4.0108352714859194E-5	1.3035214632329238E-4	2.0054176357429597E-5	5.033934173168815	1.0027088178714799E-5
72-73	4.0108352714859194E-5	1.3536569041264977E-4	2.0054176357429597E-5	5.50638550042941	1.0027088178714799E-5
74-75	4.0108352714859194E-5	1.4539277859136455E-4	2.0054176357429597E-5	5.976871518406977	1.0027088178714799E-5
76-77	4.0108352714859194E-5	1.554198667700794E-4	2.0054176357429597E-5	6.474556013069307	1.0027088178714799E-5
78-79	4.0108352714859194E-5	1.6544695494879417E-4	2.0054176357429597E-5	6.9982557880113125	1.0027088178714799E-5
80-81	4.0108352714859194E-5	1.7046049903815156E-4	2.0054176357429597E-5	7.553109725924585	1.0027088178714799E-5
82-83	4.0108352714859194E-5	1.7046049903815156E-4	2.0054176357429597E-5	8.125947246486383	1.0027088178714799E-5
84-85	4.0108352714859194E-5	1.7046049903815156E-4	2.0054176357429597E-5	8.684556328922584	1.0027088178714799E-5
86-87	4.0108352714859194E-5	1.7046049903815156E-4	2.0054176357429597E-5	9.261033682493256	1.0027088178714799E-5
88	4.0108352714859194E-5	1.7046049903815156E-4	2.0054176357429597E-5	9.71858475672028	1.0027088178714799E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	1660	0.0	53.79745	2
GGTATCA	15970	0.0	47.870857	1
TAACGCA	1955	0.0	45.439255	4
GTATCAA	26630	0.0	36.95334	1
TACCTGG	10865	0.0	36.424915	2
TATCACG	1855	0.0	33.192776	2
GTATAAC	3475	0.0	32.742825	1
ATAACGC	2905	0.0	32.521168	3
ACCTGGG	12630	0.0	30.181007	3
TCAACGC	32645	0.0	29.803679	4
ATCAACG	32895	0.0	29.505732	3
GTACCTG	13900	0.0	29.055876	1
CAACGCA	34125	0.0	28.49732	5
TATCAAC	34770	0.0	27.90109	2
AACGCAG	36105	0.0	27.298214	6
ACGCAGA	41310	0.0	23.845863	7
CGCAGAG	42290	0.0	23.282166	8
GTATCAC	3400	0.0	21.295969	1
GCAGAGT	46630	0.0	21.004354	9
GTGGTAT	8590	0.0	20.744436	1
>>END_MODULE