FastQCFastQC Report
Fri 10 Feb 2017
SRR3224566_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224566_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896063
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG15880.17721968209824532No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA13170.1469762728736707No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC12670.1413963080720887No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA12440.13882952426336095No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA10160.11338488476814688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14600.044.9339831
CCGTGCG2450.040.7225659
GGTATCA7550.040.2978361
AAACCGT3000.039.569296
ATCAACG17900.036.341853
ACCGTGC3300.035.9760978
TCAACGC18450.035.5158544
AACCGTG3350.034.017787
ACGCAGA21400.031.2856257
TATCAAC21800.030.058142
CAACGCA22050.029.9326865
CGCAGAG22200.029.7337598
AACGCAG23150.029.1257326
GTCTAAT5150.028.6156231
TACATGG29800.026.6096862
GTACCGT908.9645525E-426.3795286
TCGCACC8300.026.3468341
GTACATG31150.026.096771
TGTACCG1109.497225E-525.8998995
AATGCGG1858.996722E-925.6665657
Produced by FastQC (version 0.11.5)