##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224566_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	896063
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10489664231198	34.0	31.0	34.0	30.0	34.0
2	32.27658657929186	34.0	31.0	34.0	31.0	34.0
3	32.42258077836045	34.0	31.0	34.0	31.0	34.0
4	35.71542849107708	37.0	35.0	37.0	35.0	37.0
5	35.731947418875684	37.0	37.0	37.0	35.0	37.0
6	35.829128085860035	37.0	37.0	37.0	35.0	37.0
7	35.79107160992029	37.0	37.0	37.0	35.0	37.0
8	35.821874131617975	37.0	37.0	37.0	35.0	37.0
9	37.54433561033097	39.0	38.0	39.0	35.0	39.0
10-11	37.60820165546396	39.0	38.5	39.0	35.0	39.0
12-13	37.615389208124874	39.0	38.0	39.0	35.0	39.0
14-15	39.09272841306917	41.0	40.0	41.0	36.0	41.0
16-17	38.92766189430877	41.0	39.5	41.0	35.5	41.0
18-19	39.01230717036637	41.0	39.5	41.0	36.0	41.0
20-21	39.082192881527305	41.0	40.0	41.0	36.0	41.0
22-23	39.030853299377384	41.0	39.5	41.0	36.0	41.0
24-25	38.97379202131992	41.0	39.0	41.0	35.5	41.0
26-27	38.811031144015544	41.0	39.0	41.0	35.0	41.0
28-29	38.719367946226996	41.0	39.0	41.0	35.0	41.0
30-31	38.63471374222571	41.0	39.0	41.0	35.0	41.0
32-33	38.43589680636295	40.0	39.0	41.0	34.0	41.0
34-35	38.43317992150105	40.0	38.0	41.0	34.0	41.0
36-37	38.43454031691968	40.0	38.0	41.0	34.0	41.0
38-39	38.33031215439093	40.0	38.0	41.0	34.0	41.0
40-41	38.241230248319596	40.0	38.0	41.0	34.0	41.0
42-43	38.0915744763482	40.0	38.0	41.0	33.0	41.0
44-45	37.92305340137915	40.0	38.0	41.0	33.0	41.0
46-47	37.754057471405474	40.0	37.5	41.0	33.0	41.0
48-49	37.544480689415806	40.0	37.0	41.0	32.0	41.0
50-51	36.2853398700761	38.5	35.5	40.0	30.0	40.5
52-53	36.65609337736298	39.0	36.0	40.0	31.0	40.5
54-55	37.15206520077271	40.0	36.0	41.0	32.0	41.0
56-57	37.01704121250403	39.5	35.5	41.0	31.0	41.0
58-59	36.881033476440834	39.0	35.0	41.0	31.0	41.0
60-61	36.634787955757574	39.0	35.0	41.0	31.0	41.0
62-63	36.33977633269089	38.5	35.0	41.0	30.5	41.0
64-65	36.077304274364636	38.0	35.0	40.5	30.0	41.0
66-67	35.80841190853768	37.0	35.0	40.0	30.0	41.0
68-69	35.470933405352085	37.0	35.0	40.0	29.5	41.0
70-71	35.1410810400608	36.0	35.0	39.0	29.0	41.0
72-73	34.793160748742	36.0	34.5	39.0	29.0	41.0
74-75	34.492629424493586	35.0	34.0	38.5	29.0	40.0
76-77	34.11777687506347	35.0	34.0	37.0	28.0	39.0
78-79	33.85873426310427	35.0	34.0	37.0	28.0	39.0
80-81	33.5341571965364	35.0	34.0	36.0	28.0	39.0
82-83	33.26931700114835	35.0	34.0	36.0	27.0	37.0
84-85	33.04271072458075	35.0	34.0	35.5	27.0	37.0
86-87	32.81339704909142	35.0	33.5	35.0	27.0	36.5
88-89	32.65465430444065	35.0	33.0	35.0	26.5	36.0
90-91	32.48454740347498	35.0	33.0	35.0	26.0	36.0
92-93	32.350643872138455	35.0	33.0	35.0	25.5	36.0
94-95	32.21497037596687	35.0	33.0	35.0	25.0	35.0
96-97	32.08602799133543	35.0	33.0	35.0	25.0	35.0
98-99	32.03329899794992	35.0	33.0	35.0	25.0	35.0
100-101	30.832120062986643	34.0	31.0	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	107.0
8	346.0
9	336.0
10	463.0
11	861.0
12	1611.0
13	1915.0
14	1995.0
15	2087.0
16	2218.0
17	2402.0
18	2843.0
19	3090.0
20	3719.0
21	4529.0
22	5110.0
23	5021.0
24	4849.0
25	5061.0
26	5469.0
27	5942.0
28	6592.0
29	7574.0
30	9448.0
31	11525.0
32	15173.0
33	20934.0
34	33188.0
35	57224.0
36	118882.0
37	221173.0
38	269550.0
39	64807.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.571788632698066	18.754002787560818	13.141661515901056	24.53254706384006
2	15.679941789742227	19.953753200113386	39.34671931183333	25.019585698311055
3	19.30658816019427	25.570552037693822	28.664422774316954	26.45843702779495
4	12.465418168142195	16.998469973651407	39.168339726113004	31.367772132093393
5	13.770683534528263	38.42877119131132	34.40796015458735	13.392585119573067
6	30.25166757248095	37.29581513799811	19.239830235150876	13.212687054370061
7	27.336805559430534	31.28619304669426	22.598522648519133	18.778478745356075
8	25.73412806912014	34.615423245910165	20.919957636907228	18.73049104806247
9	26.556838079465393	15.490763484263942	19.886213357766138	38.066185078504525
10-11	25.468242746324755	25.26183984831424	28.401909240756506	20.868008164604497
12-13	26.35947472443344	22.776021328857457	28.1553305961746	22.709173350534506
14-15	23.324856066957754	24.5457610504963	25.466798551794124	26.66258433075182
16-17	23.428635246817066	27.132622291713197	26.234755416573595	23.203987044896135
18-19	23.227929172422453	26.813624122678497	27.076897316756664	22.881549388142382
20-21	24.41223640162576	25.366513174824956	26.912447129605805	23.308803293943484
22-23	24.021744956849574	26.1392874372398	26.303558346882983	23.53540925902764
24-25	23.529917334708482	25.960540326955407	26.725746789269362	23.78379554906675
26-27	23.477199705824255	26.608229555287966	26.73126777916285	23.18330295972493
28-29	23.419502869775897	26.640035354656984	26.72870099535412	23.211760780212998
30-31	23.527530988334526	25.98645407744768	26.907873665132918	23.578141269084874
32-33	23.434457175444138	26.07338992905633	26.954912768410257	23.53724012708928
34-35	23.905908401529803	25.954871476670725	26.640704950433175	23.4985151713663
36-37	23.441221137161378	25.900216943953446	27.117826742877043	23.54073517600813
38-39	23.47143308345024	26.205861706057572	26.53755110103692	23.785154109455267
40-41	24.067573019675596	25.908292164639064	26.732726307841947	23.291408507843396
42-43	23.89057967860487	26.06779229736471	26.6862976838971	23.35533034013332
44-45	23.84521071685082	26.04158586355207	26.398300804673458	23.714902614923655
46-47	23.725396540198624	25.905377188880692	26.231526131533162	24.137700139387523
48-49	23.505545927016293	26.123609612270567	26.575642560846724	23.795201899866417
50-51	23.58249364163011	26.277226043258118	26.308139048258884	23.832141266852886
52-53	24.001772196820983	26.27069748444027	25.79673527419389	23.93079504454486
54-55	23.80050286642792	25.78981611783993	26.27426866191328	24.13541235381887
56-57	23.81485666457418	26.281258282764874	26.144549068842853	23.75933598381809
58-59	23.99641488047834	26.227962034789726	26.351242374505468	23.424380710226465
60-61	23.875181867851904	26.312029703826774	26.125125082109115	23.687663346212208
62-63	23.709670307220957	26.116153534009424	26.4280575158087	23.746118642960923
64-65	24.27156552909579	25.774072405490394	25.986770302460876	23.96759176295294
66-67	23.474620161891625	26.13103131125071	25.94356786634342	24.450780660514248
68-69	23.625961567434434	26.186719014176457	26.533792824834862	23.653526593554247
70-71	24.018177293337633	25.705614448984054	26.333862685994173	23.942345571684136
72-73	23.75603054695931	25.914305132563225	26.503270417370207	23.82639390310726
74-75	23.846593375688986	25.812135977046257	26.497969450808707	23.84330119645605
76-77	23.782814378006904	25.616669810046837	26.072720333280134	24.52779547866612
78-79	23.827188880939122	25.265960712264608	26.53546198639808	24.37138842039819
80-81	23.695873560369026	26.057962663950995	26.143832450069343	24.102331325610635
82-83	23.630557602554656	26.226915432884486	26.236182756079586	23.906344208481276
84-85	23.616393519484973	25.61663241396171	26.67731272738233	24.08966133917099
86-87	23.57027405023184	26.47296765386537	26.092418325102802	23.864339970799985
88-89	23.77095848325275	25.81899819036147	25.949502042402028	24.46054128398375
90-91	23.51397167386668	25.857389491587085	26.24424845128077	24.384390383265462
92-93	23.554725972551218	25.852801098174456	26.55975090189251	24.03272202738182
94-95	23.79793608261919	25.704944853207866	26.469511630320635	24.027607433852307
96-97	23.82611490486718	26.04777789061707	26.379395198775086	23.746712005740665
98-99	23.17848792763837	26.30681152384619	26.296153695922687	24.218546852592755
100-101	23.750773884057793	25.79941036873447	26.057995108550326	24.391820638657414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	1.0
3	3.5
4	4.5
5	4.5
6	8.0
7	10.5
8	25.0
9	35.5
10	34.5
11	41.0
12	50.0
13	54.5
14	56.5
15	75.5
16	93.5
17	112.0
18	141.0
19	163.5
20	186.0
21	224.5
22	293.0
23	411.0
24	556.5
25	738.5
26	1063.5
27	1529.0
28	2065.0
29	2896.5
30	4242.5
31	6014.5
32	8214.5
33	10680.5
34	13451.0
35	16167.5
36	18527.0
37	20743.5
38	22627.5
39	24496.5
40	25817.0
41	26972.5
42	28716.5
43	30267.0
44	31752.5
45	33567.0
46	35772.0
47	36927.0
48	37863.5
49	40021.5
50	42226.0
51	41429.0
52	39394.5
53	39000.0
54	39073.5
55	37339.0
56	31621.0
57	26633.5
58	24292.0
59	22296.5
60	18388.0
61	13821.5
62	10951.5
63	7974.5
64	5112.0
65	3276.5
66	2423.5
67	2014.0
68	1562.5
69	1054.0
70	653.5
71	460.0
72	335.0
73	258.5
74	195.0
75	143.0
76	111.0
77	74.5
78	46.5
79	31.5
80	28.0
81	20.0
82	15.0
83	11.0
84	7.0
85	8.0
86	10.0
87	9.0
88	7.0
89	4.5
90	3.5
91	4.0
92	3.0
93	2.0
94	2.0
95	3.5
96	4.5
97	4.0
98	2.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15333743274747424
2	1.1159929603164062E-4
3	1.1159929603164062E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03727416487456797
16-17	0.07399033326897773
18-19	0.025165641255134964
20-21	0.04000834762734317
22-23	0.06595518395469961
24-25	0.017967486661094142
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.023380052518628715
38-39	0.004910369025392188
40-41	0.006082161633724415
42-43	0.0035711774730125
44-45	0.012220122915464648
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	5.579964801582031E-5
58-59	0.015344903204350587
60-61	0.016907293348793557
62-63	0.030634006760685353
64-65	0.04759709975749473
66-67	0.0172978908849043
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.017855887365062502
80-81	0.05814323323248476
82-83	0.049438488142016804
84-85	0.03013180992854297
86-87	0.05752943710431074
88-89	0.033926185993618754
90-91	0.0
92-93	0.003403778528965039
94-95	0.0
96-97	0.0
98-99	8.92794368253125E-4
100-101	0.0467043053892416
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	896063.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.78060194376187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.28096401264474	50.56587607408204
2	13.448717683258288	17.155346184198567
3	3.774506597677734	7.222209085217596
4	1.4550224759860306	3.7120883744036735
5	0.7051737084051833	2.24882017984987
6	0.3599065791577027	1.3773034957319077
7	0.21223019337431437	0.9475318638838328
8	0.1350594687615298	0.689133937265206
9	0.09487192367626622	0.5445889559671404
>10	0.4302709319537785	5.216091279689348
>50	0.04776427408894157	2.10165200706162
>100	0.05198757010897772	6.334411469717654
>500	0.002643435679950517	1.161990640830409
>1k	8.811452266501722E-4	0.7229564521011018
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1588	0.17721968209824532	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1317	0.1469762728736707	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	1267	0.1413963080720887	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1244	0.13882952426336095	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1016	0.11338488476814688	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	5.579964801582032E-5	0.0
20-21	0.0	0.0	0.0	1.6739894404746094E-4	0.0
22-23	0.0	0.0	0.0	2.2319859206328127E-4	0.0
24-25	0.0	0.0	0.0	3.347978880949219E-4	0.0
26-27	0.0	0.0	0.0	6.137961281740235E-4	0.0
28-29	0.0	0.0	0.0	9.485940162689455E-4	0.0
30-31	0.0	0.0	0.0	0.0029573813448384766	0.0
32-33	0.0	0.0	0.0	0.007700351426183204	0.0
34-35	0.0	0.0	0.0	0.014284709892050001	0.0
36-37	0.0	0.0	0.0	0.022822056038470512	0.0
38-39	0.0	0.0	0.0	0.04034314551543809	0.0
40-41	0.0	0.0	0.0	0.06087741598525996	0.0
42-43	0.0	0.0	0.0	0.08252767941539824	0.0
44-45	0.0	0.0	0.0	0.10813971785465978	0.0
46-47	0.0	0.0	0.0	0.13693233623082307	0.0
48-49	0.0	0.0	0.0	0.16929613207999883	0.0
50-51	0.0	0.0	0.0	0.20154832863314298	0.0
52-53	0.0	0.0	0.0	0.23497231779461936	0.0
54-55	0.0	0.0	0.0	0.2724696812612506	0.0
56-57	0.0	0.0	0.0	0.3185601905223182	0.0
58-59	0.0	0.0	0.0	0.3674406821841768	0.0
60-61	0.0	0.0	0.0	0.4244679224563451	0.0
62-63	0.0	0.0	0.0	0.4871867268261272	0.0
64-65	0.0	0.0	0.0	0.5492917350677352	0.0
66-67	0.0	0.0	0.0	0.6174231052950518	0.0
68-69	0.0	0.0	0.0	0.6950962153330736	0.0
70-71	0.0	0.0	0.0	0.7841524535663229	0.0
72-73	0.0	0.0	0.0	0.8874376020436063	0.0
74-75	0.0	0.0	0.0	0.9866493762157348	0.0
76-77	0.0	0.0	0.0	1.088985930676749	0.0
78-79	0.0	0.0	0.0	1.2138655429361551	0.0
80-81	0.0	0.0	0.0	1.3528066664955478	0.0
82-83	0.0	0.0	0.0	1.5036331150823101	0.0
84-85	0.0	0.0	0.0	1.6552407587412938	0.0
86-87	0.0	0.0	0.0	1.810196381281227	0.0
88-89	0.0	0.0	0.0	1.9952838137497029	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1460	0.0	44.933983	1
CCGTGCG	245	0.0	40.722565	9
GGTATCA	755	0.0	40.297836	1
AAACCGT	300	0.0	39.56929	6
ATCAACG	1790	0.0	36.34185	3
ACCGTGC	330	0.0	35.976097	8
TCAACGC	1845	0.0	35.515854	4
AACCGTG	335	0.0	34.01778	7
ACGCAGA	2140	0.0	31.285625	7
TATCAAC	2180	0.0	30.05814	2
CAACGCA	2205	0.0	29.932686	5
CGCAGAG	2220	0.0	29.733759	8
AACGCAG	2315	0.0	29.125732	6
GTCTAAT	515	0.0	28.615623	1
TACATGG	2980	0.0	26.609686	2
GTACCGT	90	8.9645525E-4	26.379528	6
TCGCACC	830	0.0	26.346834	1
GTACATG	3115	0.0	26.09677	1
TGTACCG	110	9.497225E-5	25.899899	5
AATGCGG	185	8.996722E-9	25.666565	7
>>END_MODULE