FastQCFastQC Report
Fri 10 Feb 2017
SRR3224566_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224566_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896063
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC13090.14608347850541759No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG11060.12342882141099454No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT10160.11338488476814688No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA9010.10055096572450821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8300.046.520341
GTATCAA15200.046.100931
CCGTGCG2400.045.509449
ACCGTGC3000.041.156378
AAACCGT2700.038.6940236
ATCAACG18300.036.0702063
TCAACGC19300.034.447344
AACCGTG3050.034.2537237
CAACGCA20650.032.195335
AACGCAG21050.031.5835446
ATACCGT1102.7378992E-630.219716
TATCAAC22800.029.5759322
ACGCAGA22650.029.5621367
GTCTAAT6350.029.277021
CGCAGAG22850.029.0955628
TATACCG1004.9603615E-528.4928685
TACATGG30850.028.0156782
GTACATG31900.027.4955941
ACATGGG31300.026.7025833
GCAGAGT26950.024.8453589
Produced by FastQC (version 0.11.5)