##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224566_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	896063
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.12147583373044	34.0	33.0	34.0	31.0	34.0
2	33.25343753731602	34.0	33.0	34.0	31.0	34.0
3	33.37402727263596	34.0	34.0	34.0	31.0	34.0
4	36.60391512650338	37.0	37.0	37.0	35.0	37.0
5	36.634332630629764	37.0	37.0	37.0	35.0	37.0
6	36.6660000468717	37.0	37.0	37.0	35.0	37.0
7	36.64331079399551	37.0	37.0	37.0	35.0	37.0
8	36.62237923003182	37.0	37.0	37.0	35.0	37.0
9	38.45845995203462	39.0	39.0	39.0	37.0	39.0
10-11	38.4768610019608	39.0	39.0	39.0	37.0	39.0
12-13	38.47636438509346	39.0	39.0	39.0	37.0	39.0
14-15	40.062532991541886	41.0	40.0	41.0	38.0	41.0
16-17	40.065550636506586	41.0	40.0	41.0	38.0	41.0
18-19	40.05760253464321	41.0	40.0	41.0	38.0	41.0
20-21	40.062636778887196	41.0	40.0	41.0	38.0	41.0
22-23	39.97725606346876	41.0	40.0	41.0	38.0	41.0
24-25	39.94440067272056	41.0	40.0	41.0	38.0	41.0
26-27	39.8848897901152	41.0	40.0	41.0	38.0	41.0
28-29	39.8452486041718	41.0	40.0	41.0	38.0	41.0
30-31	39.77671882445766	41.0	40.0	41.0	38.0	41.0
32-33	39.724534993633256	41.0	40.0	41.0	37.0	41.0
34-35	39.646174431931684	41.0	40.0	41.0	37.0	41.0
36-37	39.542698448658186	41.0	40.0	41.0	37.0	41.0
38-39	39.44374167887749	41.0	40.0	41.0	36.5	41.0
40-41	39.31943289701728	41.0	39.0	41.0	36.0	41.0
42-43	39.22133544181603	41.0	39.0	41.0	35.5	41.0
44-45	38.986157223320234	41.0	39.0	41.0	35.0	41.0
46-47	38.76433744055943	40.0	38.5	41.0	35.0	41.0
48-49	38.67760748965196	40.0	38.0	41.0	35.0	41.0
50-51	38.541704098930545	40.0	38.0	41.0	35.0	41.0
52-53	38.32549218079532	40.0	37.5	41.0	35.0	41.0
54-55	38.14867481415928	40.0	37.0	41.0	34.5	41.0
56-57	37.94026647679907	40.0	36.5	41.0	34.0	41.0
58-59	37.69304613626497	39.5	36.0	41.0	34.0	41.0
60-61	37.442593322121326	39.0	35.0	41.0	34.0	41.0
62-63	37.20938371520752	39.0	35.0	41.0	33.0	41.0
64-65	36.925011969024496	38.5	35.0	41.0	33.0	41.0
66-67	36.61570168615376	37.5	35.0	40.0	33.0	41.0
68-69	36.32026654375865	37.0	35.0	40.0	33.0	41.0
70-71	35.987583462323514	36.5	35.0	39.0	32.0	41.0
72-73	35.64401721753939	36.0	35.0	39.0	32.0	41.0
74-75	35.301530695944365	35.5	35.0	38.5	32.0	40.0
76-77	33.43419826507734	34.5	32.0	36.5	28.5	39.0
78-79	34.736924747478696	35.0	34.5	37.0	31.5	39.0
80-81	34.64995541608124	35.0	35.0	36.5	32.0	39.0
82-83	34.42604314651983	35.0	35.0	36.0	32.0	37.0
84-85	34.21288681710996	35.0	35.0	36.0	32.0	37.0
86-87	34.006942034209644	35.0	34.5	35.5	31.5	37.0
88-89	33.84200720261857	35.0	34.0	35.0	31.0	36.0
90-91	33.698969826898335	35.0	34.0	35.0	31.0	36.0
92-93	33.601804225818945	35.0	34.0	35.0	31.0	36.0
94-95	33.498048128312405	35.0	34.0	35.0	31.0	36.0
96-97	33.44869891960721	35.0	34.0	35.0	31.0	35.0
98-99	33.40143382775541	35.0	34.0	35.0	31.0	35.0
100-101	32.5488682157393	34.5	33.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	18.0
15	64.0
16	168.0
17	295.0
18	583.0
19	939.0
20	1366.0
21	2010.0
22	2702.0
23	2603.0
24	2260.0
25	2548.0
26	3151.0
27	4130.0
28	5148.0
29	5930.0
30	6979.0
31	8021.0
32	10243.0
33	13880.0
34	22448.0
35	42616.0
36	109395.0
37	241270.0
38	324075.0
39	83188.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.918512803721136	18.569881090682372	13.195749586217104	24.31585651937939
2	15.603701971847961	20.07113339129057	39.78068506343862	24.54447957342285
3	19.135596492657324	25.889474289196183	28.812259852264855	26.16266936588164
4	12.415868080704147	17.245774013657524	39.471108616246845	30.867249289391484
5	13.715553482288634	37.834616539238866	34.977897759420934	13.471932219051563
6	30.138505886304866	37.13935292496175	19.45231529479512	13.269825893938261
7	27.388252834901117	31.23351817896733	22.650974317654004	18.727254668477553
8	25.88121594128984	34.522795830203904	20.631473456665436	18.96451477184082
9	26.562306444970947	15.678919897373286	20.004955008743806	37.75381864891196
10-11	25.55417420426912	25.185003732996453	28.520985689622268	20.73983637311216
12-13	26.481787552884118	22.962280553934267	28.213697027999146	22.34223486518247
14-15	23.3609690390073	24.769407954574625	25.648587208711888	26.22103579770619
16-17	23.214104365429662	27.26370802052981	26.396414091419913	23.12577352262062
18-19	23.028737934721107	26.82110521246832	27.5631289317827	22.587027921027875
20-21	24.195389791921254	25.63469876291076	27.19297364559095	22.976937799577037
22-23	23.968001892745182	26.16229173943952	26.609586709714716	23.26011965810058
24-25	23.672745128674418	25.841461509273934	26.95924380063835	23.526549561413297
26-27	23.5134034194902	26.695013615463598	26.84104504263203	22.950537922414178
28-29	23.32360149459615	26.642694201520488	27.116563321681912	22.91714098220145
30-31	23.60792274564116	25.84919642030103	27.101075494418296	23.441805339639508
32-33	23.383701984242126	26.041057518469746	27.396993504899225	23.1782469923889
34-35	23.96161284958411	26.026501374397483	26.789478911954856	23.22240686406355
36-37	23.316252708431335	25.969748154249032	27.151664900571344	23.562334236748285
38-39	23.392998036968383	26.209708469158976	26.750016460896163	23.647277032976476
40-41	23.813671583359653	26.143251088372132	26.86412674108852	23.178950587179695
42-43	23.612346453318573	26.165459348282432	26.985546775170942	23.236647423228053
44-45	23.819603216811082	25.934604603560015	26.75381886101316	23.49197331861575
46-47	23.638044073135045	25.838517641539994	26.512961701272157	24.010476584052807
48-49	23.504009526216908	25.86257405389299	26.93483145816628	23.698584961723824
50-51	23.712953218691098	26.0270762212032	26.60387718274273	23.656093377362975
52-53	23.918351723037333	26.27019528760813	26.090297222405123	23.721155766949423
54-55	23.825668507683055	25.720066557820154	26.58457050452926	23.869694429967538
56-57	23.87010008398461	26.10191434685923	26.27942600286273	23.748559566293434
58-59	24.142067656581446	26.12565591157754	26.392597968069666	23.339678463771353
60-61	23.884006814066627	26.290865980348162	26.25525511445712	23.569872091128094
62-63	23.700230898943488	25.89862543147078	26.676137726923216	23.725005942662513
64-65	23.852898735914774	25.8708372067589	26.414604776673066	23.861659280653257
66-67	23.563075364120603	26.05804502585198	26.247596430161718	24.1312831798657
68-69	23.848769561961603	26.02573702965082	26.505502403290844	23.61999100509674
70-71	24.063709806118545	25.795619281233574	26.480057763795628	23.660613148852256
72-73	23.719035380324822	25.797516469266114	26.579771734799895	23.90367641560917
74-75	23.854963322891358	25.674701443983288	26.55153711290389	23.918798120221457
76-77	23.738631487494427	25.42908654047355	26.371935823529775	24.46034614850225
78-79	23.692816110876112	25.3971301051015	26.459919798301545	24.45013398572084
80-81	23.452728195003488	26.044679463105375	26.374588520481762	24.12800382140938
82-83	23.5558906048073	25.976894249676917	26.612458624145418	23.854756521370362
84-85	23.66740714328362	25.632990325815292	26.782263604095558	23.91733892680553
86-87	23.371738371074354	26.332690893385845	26.424425514723854	23.871145220815947
88-89	23.87633458808142	25.67113026651028	25.995716819018305	24.45681832639
90-91	23.65882756011575	25.878091161000956	26.183147836703448	24.279933442179846
92-93	23.768250669874774	25.849019544384717	26.531672438210258	23.85105734753025
94-95	23.75335216385455	25.720959352188405	26.365947483603275	24.159741000353772
96-97	23.656762973139163	26.025904428594863	26.480838958867846	23.836493639398125
98-99	22.869653138228006	26.31165442608388	26.553322701640397	24.265369734047717
100-101	23.4390529901167	25.927243285498914	26.311295996426377	24.32240772795801
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.5
2	2.5
3	5.5
4	5.5
5	5.0
6	8.0
7	17.5
8	32.5
9	45.5
10	48.0
11	53.5
12	63.0
13	72.5
14	84.5
15	94.5
16	110.5
17	123.5
18	147.5
19	197.0
20	230.5
21	243.5
22	320.5
23	440.5
24	587.0
25	822.0
26	1173.0
27	1609.5
28	2223.5
29	3121.0
30	4429.5
31	6157.0
32	8400.0
33	10867.5
34	13563.0
35	16453.5
36	18942.5
37	20974.5
38	22885.0
39	24662.5
40	25857.5
41	27069.0
42	28770.0
43	30504.5
44	32106.0
45	33981.5
46	35955.5
47	37328.0
48	38481.0
49	40617.5
50	42942.0
51	41690.0
52	38579.0
53	38295.0
54	38806.0
55	36669.0
56	31030.5
57	26382.0
58	24583.0
59	22567.5
60	18274.0
61	13476.0
62	10524.0
63	7380.5
64	4458.0
65	2827.0
66	2092.5
67	1715.0
68	1270.0
69	840.0
70	531.5
71	344.0
72	243.0
73	204.0
74	156.0
75	101.5
76	72.0
77	47.5
78	28.5
79	15.5
80	7.5
81	5.0
82	3.5
83	1.5
84	1.5
85	1.5
86	0.5
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47887257927176996
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.92794368253125E-4
22-23	0.0011159929603164064
24-25	3.347978880949219E-4
26-27	0.0025667838087277345
28-29	0.003905975361107422
30-31	0.0011159929603164064
32-33	3.347978880949219E-4
34-35	0.0037943760650757815
36-37	0.002064586976585352
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.009206941922610352
46-47	0.08180228399119259
48-49	0.001060193312300586
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0073097538900724615
58-59	0.04051054445948555
60-61	0.030020210632511327
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.789982400791016E-4
78-79	0.008035149314278124
80-81	0.006528558817850977
82-83	0.0010043936642847656
84-85	0.002064586976585352
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0684103684673957
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	896063.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.14259483066355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.09436526324279	48.52986499111543
2	14.047677421314997	17.459182526092768
3	4.075529111069987	7.597918628093899
4	1.601102103875083	3.9798655729452905
5	0.7452628927027892	2.3156284991778855
6	0.3899010951157535	1.4537679466686169
7	0.22133448695237745	0.9628009541314066
8	0.15730574223801042	0.7820309603546783
9	0.08991453613281913	0.5028770329459953
>10	0.46697906525575833	5.47775838982428
>50	0.051989117075723124	2.303837069729499
>100	0.05360267243873344	6.381900489324126
>500	0.004496868379589422	1.8690537811128936
>1k	5.396242055507306E-4	0.38351315848324485
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	1309	0.14608347850541759	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1106	0.12342882141099454	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1016	0.11338488476814688	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	901	0.10055096572450821	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	5.579964801582032E-5	0.0
20-21	0.0	0.0	0.0	1.6739894404746094E-4	0.0
22-23	0.0	0.0	0.0	2.2319859206328127E-4	0.0
24-25	0.0	0.0	0.0	3.347978880949219E-4	0.0
26-27	0.0	0.0	0.0	6.137961281740235E-4	0.0
28-29	0.0	0.0	0.0	9.485940162689455E-4	0.0
30-31	0.0	0.0	0.0	0.0030131809928542973	0.0
32-33	0.0	0.0	0.0	0.008090948962293947	0.0
34-35	0.0	0.0	0.0	0.014786906724192386	0.0
36-37	0.0	0.0	0.0	0.023547451462676172	0.0
38-39	0.0	0.0	0.0	0.041012741291627935	0.0
40-41	0.0	0.0	0.0	0.06137961281740235	0.0
42-43	0.0	0.0	0.0	0.08314147554357226	0.0
44-45	0.0	0.0	0.0	0.10925571081497618	0.0
46-47	0.0	0.0	0.0	0.13888532391137676	0.0
48-49	0.0	0.0	0.0	0.17158391764864747	0.0
50-51	0.0	0.0	0.0	0.20444991032996562	0.0
52-53	0.0	0.0	0.0	0.2381528977315211	0.0
54-55	0.0	0.0	0.0	0.27643145627037385	0.0
56-57	0.0	0.0	0.0	0.3230241623635838	0.0
58-59	0.0	0.0	0.0	0.3730206469857588	0.0
60-61	0.0	0.0	0.0	0.43077328268213283	0.0
62-63	0.0	0.0	0.0	0.49427328212413635	0.0
64-65	0.0	0.0	0.0	0.5571036857899501	0.0
66-67	0.0	0.0	0.0	0.6265742475696463	0.0
68-69	0.0	0.0	0.0	0.7053633505679846	0.0
70-71	0.0	0.0	0.0	0.7949217856333762	0.0
72-73	0.0	0.0	0.0	0.8983185334066912	0.0
74-75	0.0	0.0	0.0	0.9987021001871521	0.0
76-77	0.0	0.0	0.0	1.1031032416247517	0.0
78-79	0.0	0.0	0.0	1.2290430471964582	0.0
80-81	0.0	0.0	0.0	1.3688211654760882	0.0
82-83	0.0	0.0	0.0	1.5215448020953883	0.0
84-85	0.0	0.0	0.0	1.6749380344908784	0.0
86-87	0.0	0.0	0.0	1.832516240487555	0.0
88-89	0.0	0.0	0.0	2.019166063100474	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	830	0.0	46.52034	1
GTATCAA	1520	0.0	46.10093	1
CCGTGCG	240	0.0	45.50944	9
ACCGTGC	300	0.0	41.15637	8
AAACCGT	270	0.0	38.694023	6
ATCAACG	1830	0.0	36.070206	3
TCAACGC	1930	0.0	34.44734	4
AACCGTG	305	0.0	34.253723	7
CAACGCA	2065	0.0	32.19533	5
AACGCAG	2105	0.0	31.583544	6
ATACCGT	110	2.7378992E-6	30.21971	6
TATCAAC	2280	0.0	29.575932	2
ACGCAGA	2265	0.0	29.562136	7
GTCTAAT	635	0.0	29.27702	1
CGCAGAG	2285	0.0	29.095562	8
TATACCG	100	4.9603615E-5	28.492868	5
TACATGG	3085	0.0	28.015678	2
GTACATG	3190	0.0	27.495594	1
ACATGGG	3130	0.0	26.702583	3
GCAGAGT	2695	0.0	24.845358	9
>>END_MODULE