##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224563_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	987148
Sequences flagged as poor quality	0
Sequence length	101
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24302232289383	34.0	31.0	34.0	31.0	34.0
2	32.405117571022785	34.0	31.0	34.0	31.0	34.0
3	32.550241706410795	34.0	31.0	34.0	31.0	34.0
4	35.82955443358038	37.0	37.0	37.0	35.0	37.0
5	35.832335171625736	37.0	37.0	37.0	35.0	37.0
6	35.92521182234072	37.0	37.0	37.0	35.0	37.0
7	35.898199662056754	37.0	37.0	37.0	35.0	37.0
8	35.93652015705852	37.0	37.0	37.0	35.0	37.0
9	37.67573352729276	39.0	39.0	39.0	35.0	39.0
10-11	37.74883806683496	39.0	39.0	39.0	35.0	39.0
12-13	37.73347107019414	39.0	39.0	39.0	35.0	39.0
14-15	39.224191813183026	41.0	40.0	41.0	36.0	41.0
16-17	39.05356744885266	41.0	40.0	41.0	36.0	41.0
18-19	39.1181687041862	41.0	40.0	41.0	36.0	41.0
20-21	39.17709958385166	41.0	40.0	41.0	36.0	41.0
22-23	39.129991146211104	41.0	40.0	41.0	36.0	41.0
24-25	39.057335374229595	41.0	39.5	41.0	35.5	41.0
26-27	38.871415937630424	41.0	39.0	41.0	35.0	41.0
28-29	38.79021788019628	41.0	39.0	41.0	35.0	41.0
30-31	38.722908317699066	41.0	39.0	41.0	35.0	41.0
32-33	38.533586149189375	41.0	39.0	41.0	35.0	41.0
34-35	38.52758198365392	40.0	38.0	41.0	35.0	41.0
36-37	38.49833611575974	40.0	38.0	41.0	35.0	41.0
38-39	38.355152925397206	40.0	38.0	41.0	34.5	41.0
40-41	38.24220684233772	40.0	38.0	41.0	34.0	41.0
42-43	38.065987065769264	40.0	38.0	41.0	33.5	41.0
44-45	37.85932555199423	40.0	37.0	41.0	33.0	41.0
46-47	37.65122453775928	40.0	37.0	41.0	33.0	41.0
48-49	37.395621527876266	40.0	36.0	41.0	32.5	41.0
50-51	36.03205294444197	38.0	34.5	40.0	30.0	40.5
52-53	36.32725589273341	38.5	35.0	40.0	31.0	40.5
54-55	36.70851179357098	39.0	35.0	41.0	31.5	41.0
56-57	36.488161856175566	38.5	35.0	41.0	31.0	41.0
58-59	36.27491014518593	37.0	35.0	41.0	31.0	41.0
60-61	35.97532436878766	37.0	35.0	40.0	31.0	41.0
62-63	35.650742847070546	36.0	35.0	40.0	30.5	41.0
64-65	35.37085725747305	36.0	35.0	39.5	30.0	41.0
66-67	35.10837280731967	35.0	35.0	39.0	30.0	41.0
68-69	34.78999856151256	35.0	34.5	39.0	29.5	41.0
70-71	34.49563793878932	35.0	34.0	37.5	29.0	40.5
72-73	34.19033822689202	35.0	34.0	37.0	29.0	39.5
74-75	33.94035240916256	35.0	34.0	36.5	29.0	39.0
76-77	33.66303127798466	35.0	34.0	36.0	29.0	39.0
78-79	33.482298500326195	35.0	34.0	36.0	29.0	37.5
80-81	33.233466511607176	35.0	34.0	35.0	28.5	37.0
82-83	33.03724669451795	35.0	34.0	35.0	27.5	37.0
84-85	32.871550669200566	35.0	33.5	35.0	27.0	36.0
86-87	32.704988512360856	35.0	33.0	35.0	27.0	36.0
88-89	32.607632290193564	35.0	33.0	35.0	27.0	36.0
90-91	32.468461669374804	35.0	33.0	35.0	27.0	35.0
92-93	32.363414604496995	35.0	33.0	35.0	26.0	35.0
94-95	32.25019247367163	35.0	33.0	35.0	25.0	35.0
96-97	32.132740480657404	35.0	33.0	35.0	25.0	35.0
98-99	32.10723771916673	35.0	33.0	35.0	25.0	35.0
100-101	30.799426732364346	34.0	30.5	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	7.0
7	146.0
8	555.0
9	428.0
10	513.0
11	1083.0
12	1652.0
13	1963.0
14	1888.0
15	2053.0
16	2162.0
17	2242.0
18	2564.0
19	2897.0
20	3253.0
21	4203.0
22	5191.0
23	5306.0
24	5287.0
25	5774.0
26	6018.0
27	6407.0
28	7013.0
29	8043.0
30	10114.0
31	12751.0
32	17190.0
33	24604.0
34	42397.0
35	77777.0
36	173967.0
37	283593.0
38	225918.0
39	42187.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.064343288638945	17.019981619101525	12.65289519438916	25.262779897870374
2	17.385336342676073	18.535315879685722	36.34379039414556	27.735557383492647
3	21.40651655070972	24.062248011443067	26.516591230494313	28.0146442073529
4	13.91199698525449	15.29973215769064	35.72189783092302	35.06637302613185
5	15.252322853310748	37.70093238298614	32.26648891554255	14.780255848160559
6	32.81544408741141	34.14634887575115	18.113190727226314	14.925016309611122
7	29.789251459760845	29.36854453435554	20.164250953251184	20.677953052632432
8	28.04108401171861	32.65184146652781	19.112939498433875	20.194135023319703
9	27.978985927135547	14.589808215181513	18.255216036501114	39.17598982118183
10-11	28.079224189280634	23.316260580986842	25.62356404510772	22.9809511846248
12-13	28.212537532365968	21.137863825890342	25.81644292446523	24.833155717278462
14-15	25.243224646291246	22.806240616704507	23.21579945550918	28.734735281495066
16-17	25.44872103543795	25.4268748558583	24.236688909895914	24.887715198807836
18-19	25.404020267001176	25.15769949980976	24.65715462530315	24.78112560788591
20-21	26.4933896240507	23.325668688727603	24.415777750303512	25.765163936918185
22-23	26.556040992995513	24.29797974678412	23.651812956787058	25.494166303433314
24-25	25.77656915932402	23.937737687129143	24.437846351339505	25.847846802207325
26-27	25.585677122376786	25.275237350427698	24.52747713615385	24.611608391041667
28-29	25.21126518009458	25.29777703039463	24.624929595156956	24.866028194353834
30-31	25.432052741838103	24.323252440363554	24.655320174887656	25.58937464291069
32-33	25.144456555653257	24.58684006856115	25.00167148188519	25.26703189390041
34-35	26.081144873919616	23.77399336269739	24.495567027436614	25.64929473594638
36-37	24.944905632848098	24.296033306888038	25.450912565365986	25.308148494897882
38-39	25.216567639714114	24.60305641243839	24.30004913357748	25.88032681427001
40-41	25.955155097149095	24.045300787870623	24.82349651653191	25.17604759844837
42-43	25.64400894523702	24.52560105559875	24.87738288501622	24.95300711414801
44-45	25.705526538010115	23.855563035784026	24.663832960250605	25.775077465955253
46-47	25.478195772062545	23.859137636909562	24.584459473148907	26.078207117878982
48-49	24.808995206392556	24.544293256938168	24.977966829695244	25.668744706974028
50-51	25.217039390243407	24.55756381008724	24.629893389846305	25.595503409823046
52-53	25.460822490649832	24.554271497283082	23.91130813211393	26.07359787995316
54-55	25.592109795086447	23.729217908560823	24.40500309983913	26.273669196513595
56-57	25.47081363220795	24.23903216084729	24.45055070290914	25.83960350403562
58-59	25.808437076531455	24.548220007345396	24.723699041298868	24.919643874824278
60-61	25.4287989576505	24.864159205512458	24.21152562155459	25.495516215282453
62-63	25.05351507263104	24.15709644062069	25.038163529902324	25.751224956845952
64-65	26.084537389549705	23.4837962317808	24.25900692156021	26.172659457109283
66-67	25.009764452529566	24.412701741694644	23.96594321065331	26.611590595122486
68-69	25.21293666197976	24.4483603269216	25.178595306884073	25.160107704214568
70-71	25.7694894787813	24.033123705867812	24.755305182201656	25.442081633149233
72-73	25.280606352846785	24.287847414977286	25.219825193385386	25.211721038790536
74-75	25.460721188717393	24.110417080316225	25.132452276659627	25.29640945430675
76-77	25.28754553521863	23.52534776953405	24.507470004497804	26.679636690749515
78-79	25.454982043881923	23.0430516231429	25.173570742140623	26.328395590834553
80-81	25.24609870706956	24.353788176720766	24.33169199956213	26.068421116647546
82-83	24.775774356968135	24.73579152576815	24.877834460943767	25.61059965631995
84-85	25.260252929317907	23.581876759655337	25.369283795895438	25.78858651513133
86-87	25.1380515473531	25.01520391490673	24.466443946206525	25.38030059153365
88-89	25.50727516209954	24.00446283579571	24.003753484057068	26.484508518047683
90-91	25.008914570054337	23.910497716654444	24.53345395016755	26.547133763123664
92-93	24.984550985799064	24.155572079528486	25.10424285647714	25.75563407819531
94-95	25.335106792497175	23.886235903836102	25.01838630073707	25.760271002929652
96-97	25.743708136976423	24.398469125197032	24.77708509767532	25.080737640151224
98-99	24.31414341307705	24.576314540519267	24.994035809455887	26.115506236947795
100-101	25.323931014982158	23.96030432539751	24.315830332611405	26.39993432700893
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	2.0
3	4.5
4	3.5
5	1.5
6	2.0
7	2.5
8	7.5
9	13.5
10	14.5
11	17.5
12	20.0
13	19.0
14	23.0
15	28.0
16	29.0
17	33.0
18	40.0
19	50.5
20	64.0
21	80.5
22	93.5
23	113.5
24	155.0
25	195.0
26	264.0
27	365.0
28	439.5
29	513.5
30	601.5
31	773.0
32	1078.5
33	1581.5
34	2426.0
35	4124.5
36	7071.5
37	10763.5
38	14257.0
39	17362.0
40	19757.5
41	21519.0
42	22732.0
43	23878.5
44	25491.0
45	28331.0
46	33143.5
47	35597.0
48	38228.0
49	43852.0
50	49272.5
51	51100.0
52	51513.5
53	53981.0
54	57449.0
55	57920.0
56	50138.0
57	43581.0
58	41819.0
59	39832.5
60	34117.5
61	26579.0
62	21639.0
63	15941.0
64	10255.0
65	6695.5
66	5072.5
67	4404.0
68	3340.5
69	2129.5
70	1365.0
71	942.5
72	716.0
73	577.0
74	425.5
75	303.0
76	249.5
77	171.0
78	101.5
79	73.5
80	58.5
81	40.5
82	23.0
83	21.0
84	21.5
85	17.0
86	12.5
87	11.0
88	11.0
89	6.5
90	4.5
91	5.5
92	6.5
93	5.5
94	6.5
95	6.0
96	5.0
97	7.5
98	6.0
99	2.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13594719332865993
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.035911535048442585
16-17	0.07146851333336035
18-19	0.02264098189937071
20-21	0.03707650727145271
22-23	0.06463063289395309
24-25	0.01646156402079526
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.020766896149310942
38-39	0.004355983094733515
40-41	0.0057742101488327995
42-43	0.003089708939287726
44-45	0.01028214614221981
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	5.065096621783157E-5
58-59	0.013726411845032356
60-61	0.014891384068042482
62-63	0.028009984318460857
64-65	0.045433916697394924
66-67	0.015144638899131639
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01605635629105261
80-81	0.05591866670448606
82-83	0.04806776694072216
84-85	0.027503474656282546
86-87	0.056729082163971364
88-89	0.03358159060242233
90-91	0.0
92-93	0.0030390579730698946
94-95	0.0
96-97	0.0
98-99	6.584625608318104E-4
100-101	0.044674152204127446
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	987148.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.476149637546236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.30141881827113	35.275452780982334
2	14.480244046701241	13.749324662987522
3	5.0176799572723	7.146603734543289
4	2.267481505918935	4.306051651015041
5	1.1588906469869382	2.7509832884952323
6	0.6886222062488581	1.9615878544564802
7	0.41999423025826177	1.3957796245853098
8	0.2872508224837322	1.0910050425396718
9	0.20348020865157088	0.8694411150798997
>10	0.9461967973703918	8.116572268055744
>50	0.0969845347789304	3.292312651878867
>100	0.11188479443652811	11.545295285363606
>500	0.014903572965886193	4.781083488475199
>1k	0.004967857655295397	3.7185065515418594
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	4208	0.42627853168927043	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2648	0.268247517089636	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2360	0.23907256054816503	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	2002	0.2028064687361976	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1954	0.19794397597928579	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1620	0.1641091305457743	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1589	0.16096877064026874	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1559	0.15792971266719882	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1555	0.15752450493745618	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1480	0.14992686000478145	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1466	0.14850863295068217	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1403	0.14212661120723538	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1305	0.1321990218285404	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1268	0.12845085032842088	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	1217	0.12328445177420204	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1208	0.12237273438228106	No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG	1138	0.11528159911178466	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1108	0.11224254113871476	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1049	0.10626572712501064	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	1047	0.10606312326013931	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	1046	0.10596182132770364	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC	1024	0.10373317881411906	No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC	1008	0.10211234789514845	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1000	0.10130193243566314	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	999	0.10120063050322747	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.0130193243566314E-4	0.0
6	0.0	0.0	0.0	1.0130193243566314E-4	0.0
7	0.0	0.0	0.0	1.0130193243566314E-4	0.0
8	0.0	0.0	0.0	1.0130193243566314E-4	0.0
9	0.0	0.0	0.0	1.0130193243566314E-4	0.0
10-11	0.0	0.0	0.0	1.0130193243566314E-4	0.0
12-13	0.0	0.0	0.0	1.0130193243566314E-4	0.0
14-15	0.0	0.0	0.0	1.0130193243566314E-4	0.0
16-17	0.0	0.0	0.0	1.5195289865349473E-4	0.0
18-19	0.0	0.0	0.0	2.5325483108915785E-4	0.0
20-21	0.0	0.0	0.0	3.5455676352482096E-4	0.0
22-23	0.0	0.0	0.0	8.104154594853051E-4	0.0
24-25	0.0	0.0	0.0	0.0012156231892279576	0.0
26-27	0.0	0.0	0.0	0.0016208309189706103	0.0
28-29	0.0	0.0	0.0	0.0025325483108915785	0.0
30-31	0.0	0.0	0.0	0.0061794178785754515	0.0
32-33	0.0	0.0	0.0	0.016360262088359598	0.0
34-35	0.0	0.0	0.0	0.025629388906222773	0.0
36-37	0.0	0.0	0.0	0.0441169915757313	0.0
38-39	0.0	0.0	0.0	0.0786102995700746	0.0
40-41	0.0	0.0	0.0	0.11872586481459721	0.0
42-43	0.0	0.0	0.0	0.1601077042145656	0.0
44-45	0.0	0.0	0.0	0.20447795062138607	0.0
46-47	0.0	0.0	0.0	0.25695235162305957	0.0
48-49	0.0	0.0	0.0	0.31515031180734804	0.0
50-51	0.0	0.0	0.0	0.37486780097817146	0.0
52-53	0.0	0.0	0.0	0.4424868408789766	0.0
54-55	0.0	0.0	0.0	0.5110175981717027	0.0
56-57	0.0	0.0	0.0	0.5892226900120346	0.0
58-59	0.0	0.0	0.0	0.6777605789608042	0.0
60-61	0.0	0.0	0.0	0.7792651152613388	0.0
62-63	0.0	0.0	0.0	0.8951545259677374	0.0
64-65	0.0	0.0	0.0	1.0022813195184512	0.0
66-67	0.0	0.0	0.0	1.1219189017249693	0.0
68-69	0.0	0.0	0.0	1.2671352218714924	0.0
70-71	0.0	0.0	0.0	1.4231401978224136	0.0
72-73	0.0	0.0	0.0	1.59748082354419	5.065096621783157E-5
74-75	0.0	0.0	0.0	1.7723786098943624	1.0130193243566314E-4
76-77	0.0	0.0	0.0	1.9471750943120991	1.0130193243566314E-4
78-79	0.0	0.0	0.0	2.1438527961359393	1.0130193243566314E-4
80-81	0.0	0.0	0.0	2.3753783627176475	1.0130193243566314E-4
82-83	0.0	0.0	0.0	2.6293929583000724	1.0130193243566314E-4
84-85	0.0	0.0	0.0	2.886699866686657	1.0130193243566314E-4
86-87	0.0	0.0	0.0	3.1605696410264725	1.0130193243566314E-4
88-89	0.0	0.0	0.0	3.4549024057182915	1.0130193243566314E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	725	0.0	51.148407	1
GTATCAA	1540	0.0	46.306934	1
GCGTAAT	50	0.0016087407	38.03343	1
AAACCGT	125	1.3460522E-10	37.991047	6
ATCAACG	2060	0.0	34.348698	3
TCAACGC	2170	0.0	32.60752	4
GTACATG	4760	0.0	31.561352	1
TACATGG	4840	0.0	30.808853	2
TCGCACC	1320	0.0	30.614027	1
CGCACCA	1370	0.0	30.157125	2
AAATGCG	300	0.0	30.076244	6
ACATGGG	4870	0.0	29.741447	3
CATGGGG	3925	0.0	27.46486	4
GCCCATA	1060	0.0	27.358952	1
CGCAGAG	2610	0.0	27.29242	8
TATCACG	70	0.008400658	27.136461	2
AATGCGG	315	0.0	27.13646	7
ACCGTGC	195	5.366019E-10	26.788557	8
TATCAAC	2645	0.0	26.751728	2
ACGCAGA	2710	0.0	26.285316	7
>>END_MODULE