##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224563_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	987148
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.17122153922208	34.0	33.0	34.0	31.0	34.0
2	33.29884373974318	34.0	34.0	34.0	31.0	34.0
3	33.409409733900084	34.0	34.0	34.0	31.0	34.0
4	36.61901761438001	37.0	37.0	37.0	35.0	37.0
5	36.649842779400856	37.0	37.0	37.0	35.0	37.0
6	36.69088525732717	37.0	37.0	37.0	36.0	37.0
7	36.67134917965695	37.0	37.0	37.0	35.0	37.0
8	36.65990611336902	37.0	37.0	37.0	35.0	37.0
9	38.5043336966696	39.0	39.0	39.0	37.0	39.0
10-11	38.52932336387249	39.0	39.0	39.0	37.5	39.0
12-13	38.50988706860572	39.0	39.0	39.0	37.0	39.0
14-15	40.106261168538055	41.0	40.0	41.0	38.0	41.0
16-17	40.110821275026645	41.0	40.0	41.0	38.0	41.0
18-19	40.09233367235713	41.0	40.0	41.0	38.0	41.0
20-21	40.08830590752349	41.0	40.0	41.0	38.0	41.0
22-23	40.01153525104645	41.0	40.0	41.0	38.0	41.0
24-25	39.96348470543424	41.0	40.0	41.0	38.0	41.0
26-27	39.887429747109856	41.0	40.0	41.0	38.0	41.0
28-29	39.860119759144524	41.0	40.0	41.0	38.0	41.0
30-31	39.80425934105119	41.0	40.0	41.0	37.5	41.0
32-33	39.763649928886046	41.0	40.0	41.0	37.0	41.0
34-35	39.684072195861205	41.0	40.0	41.0	37.0	41.0
36-37	39.55503835291162	41.0	40.0	41.0	36.5	41.0
38-39	39.427406022197275	41.0	39.0	41.0	36.0	41.0
40-41	39.28023913334171	41.0	39.0	41.0	35.0	41.0
42-43	39.158740634636345	41.0	39.0	41.0	35.0	41.0
44-45	38.885322666915194	40.5	38.0	41.0	35.0	41.0
46-47	38.637028084947744	40.0	38.0	41.0	35.0	41.0
48-49	38.49362912146912	40.0	37.0	41.0	35.0	41.0
50-51	38.27943327646918	40.0	37.0	41.0	35.0	41.0
52-53	37.97103777751664	39.5	36.0	41.0	34.5	41.0
54-55	37.72683478060028	39.0	35.5	41.0	34.0	41.0
56-57	37.431475320823225	39.0	35.0	41.0	34.0	41.0
58-59	37.1091933529724	38.0	35.0	41.0	34.0	41.0
60-61	36.803607463115966	37.0	35.0	40.5	33.0	41.0
62-63	36.54927832503333	36.5	35.0	40.0	33.0	41.0
64-65	36.248644073634345	36.0	35.0	40.0	33.0	41.0
66-67	35.94952833820258	35.5	35.0	39.0	33.0	41.0
68-69	35.66969390608095	35.0	35.0	39.0	33.0	41.0
70-71	35.38140886675554	35.0	35.0	38.5	32.5	40.5
72-73	35.09590253943684	35.0	35.0	37.0	32.0	39.5
74-75	34.80664500156005	35.0	35.0	37.0	32.0	39.0
76-77	33.03886853845624	34.5	32.0	35.0	28.5	38.0
78-79	34.37881351124654	35.0	34.5	36.0	31.5	37.5
80-81	34.35617050330852	35.0	35.0	35.5	32.0	37.0
82-83	34.20808885800305	35.0	35.0	35.0	32.0	37.0
84-85	34.06606912033454	35.0	35.0	35.0	32.0	36.0
86-87	33.92997250665554	35.0	35.0	35.0	32.0	36.0
88-89	33.83186766320755	35.0	34.0	35.0	31.5	36.0
90-91	33.72606336638478	35.0	34.0	35.0	31.0	36.0
92-93	33.66125393558008	35.0	34.0	35.0	31.0	35.0
94-95	33.58134646476516	35.0	34.0	35.0	31.0	35.0
96-97	33.54986840878976	35.0	34.0	35.0	31.0	35.0
98-99	33.53154643477979	35.0	34.0	35.0	31.0	35.0
100-101	32.640450570735084	34.5	32.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	13.0
15	48.0
16	113.0
17	202.0
18	395.0
19	558.0
20	841.0
21	1235.0
22	1678.0
23	1865.0
24	2026.0
25	2686.0
26	3479.0
27	4991.0
28	6630.0
29	7935.0
30	8103.0
31	8982.0
32	10776.0
33	14753.0
34	26498.0
35	57407.0
36	163723.0
37	327369.0
38	278817.0
39	56013.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.51290857305089	17.08280800513677	12.69762770435903	24.70665571745331
2	17.211097018886733	18.776515780815036	37.137896242508724	26.87449095778951
3	20.93779250932991	24.828901036116164	26.846329020572394	27.386977433981528
4	13.801375275034747	15.598167650646102	36.62601757791132	33.97443949640783
5	15.081325191359351	37.15076158792805	33.133126947529654	14.634786273182948
6	32.47628521761681	34.09174713416833	18.62689282660756	14.805074821607297
7	29.7030435152581	29.193697398971587	20.582526632278036	20.520732453492283
8	27.983139306365405	32.33709636245021	19.225891153099635	20.453873178084745
9	27.91668523868761	14.826753435148529	18.584953826579195	38.67160749958467
10-11	28.08352952140915	23.27634761960719	25.98034945114613	22.659773407837527
12-13	28.350814670140643	21.327399741477468	26.035660306256002	24.28612528212588
14-15	25.14248116797076	23.246919408234632	23.546823779210413	28.063775644584194
16-17	25.193385389019678	25.585930377207877	24.600262574608873	24.62042165916357
18-19	25.09790831769907	25.11259709790224	25.342096625835236	24.447397958563457
20-21	26.18394940540409	23.756079425330324	24.89492382547174	25.165047343793844
22-23	26.448747008395486	24.261564371731396	24.221802940319865	25.067885679553253
24-25	26.064586321752447	23.820030309661004	24.598741419691557	25.516641948894993
26-27	25.58567721563548	25.328921875767698	24.653121965624468	24.432278942972356
28-29	25.138940948830424	25.179210017120685	25.14714672123674	24.534702312812147
30-31	25.33023347692962	24.144234495019713	25.162273937146868	25.363258090903795
32-33	25.21251911443742	24.57532899118824	25.493733706192543	24.718418188181797
34-35	26.135175602462123	23.955543218831423	24.597767638251227	25.311513540455227
36-37	24.771295097071373	24.26128354342797	25.519875964217643	25.44754539528301
38-39	25.208175471155286	24.417412586562502	24.581268462277187	25.793143480005025
40-41	25.604164725046296	24.228332529671338	25.12845085032842	25.03905189495395
42-43	25.438181508750464	24.488577194098553	25.305577279192175	24.767664017958808
44-45	25.723189266183493	23.90394555286976	25.068079174458962	25.304786006487785
46-47	25.337651350681433	23.8859723066333	24.851190099041016	25.925186243644248
48-49	24.856049079058533	24.089036835632562	25.386469221246795	25.668444864062113
50-51	25.42536681429735	24.211617710819453	24.930506874349135	25.432508600534064
52-53	25.52482051811939	24.45988287458707	24.40467326548123	25.610623341812317
54-55	25.719598277056733	23.732256966533892	24.732512247403633	25.81563250900574
56-57	25.740306329461454	24.038965650515742	24.610553712004517	25.61017430801829
58-59	25.986459912026415	24.21675288199369	24.877441479731672	24.91934572624822
60-61	25.442830630235346	24.75418283112676	24.394253904929922	25.40873263370797
62-63	25.216684833479885	24.03246524330698	25.13224967279476	25.61860025041838
64-65	25.548955171868858	23.889224310842955	24.564705596323954	25.997114920964233
66-67	25.102517555624893	24.232941767597158	24.500277567294873	26.164263109483077
68-69	25.447703890399413	24.271537803855146	25.125614396220225	25.15514390952522
70-71	25.89257132669063	24.05515687617257	24.872207612232412	25.180064184904396
72-73	25.256445841960883	24.09653871557254	25.267335799697715	25.379679642768867
74-75	25.415489875884873	23.85989740140283	25.205490969945743	25.519121752766555
76-77	25.333220550343842	23.25840186841568	24.868902437480152	26.53947514376032
78-79	25.46231759552301	23.145765541611116	24.924829701790717	26.46708716107516
80-81	24.92898403609759	24.30837947851387	24.748050353458915	26.01458613192963
82-83	24.74909815132418	24.3571067499106	25.284939608302377	25.608855490462844
84-85	25.170759578111774	23.64680157243169	25.422599881373692	25.75983896808284
86-87	24.82221510857541	24.76102874138427	24.834320689501475	25.582435460538843
88-89	25.45879645200112	23.552952546122768	24.138477715600903	26.84977328627521
90-91	25.232690538804718	23.90573652582997	24.391327338960316	26.470245596404997
92-93	25.20675724410119	24.052168469165718	25.051106824913795	25.6899674618193
94-95	25.23826214508868	23.735701232236707	24.83234530181898	26.19369132085564
96-97	25.572406569227713	24.229598803826782	24.73443698411991	25.463557642825595
98-99	23.771511465352713	24.565667964682095	25.408651995445464	26.254168574519728
100-101	24.798124273946833	23.961123083878526	24.748453620787878	26.49229902138676
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	0.5
3	1.0
4	3.0
5	2.5
6	2.5
7	7.0
8	12.0
9	17.0
10	19.0
11	16.5
12	21.5
13	25.5
14	24.5
15	26.5
16	34.0
17	40.0
18	48.5
19	57.0
20	61.0
21	73.5
22	95.5
23	120.0
24	159.0
25	217.5
26	294.0
27	405.5
28	474.5
29	558.5
30	683.0
31	857.5
32	1201.0
33	1761.0
34	2730.5
35	4669.5
36	7937.0
37	11731.0
38	15246.5
39	18421.5
40	20585.0
41	21760.0
42	22857.5
43	24493.5
44	26371.5
45	28993.0
46	32443.5
47	35691.5
48	38840.0
49	43970.0
50	50085.5
51	51588.0
52	50317.5
53	52567.0
54	57447.5
55	57632.0
56	49477.5
57	43217.5
58	41853.5
59	40025.5
60	34078.0
61	26069.0
62	20911.0
63	15102.0
64	9454.5
65	6067.0
66	4604.0
67	3951.5
68	2842.5
69	1787.0
70	1140.0
71	762.0
72	580.0
73	472.0
74	346.0
75	241.5
76	193.0
77	116.5
78	63.5
79	34.0
80	17.0
81	12.5
82	10.0
83	5.5
84	3.5
85	3.0
86	1.5
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4486662587575521
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.091135270496419E-4
22-23	0.001063670290574463
24-25	4.052077297426526E-4
26-27	0.0021779915473667576
28-29	0.003849473432555199
30-31	5.571606283961473E-4
32-33	1.5195289865349473E-4
34-35	0.0032416618379412206
36-37	0.0013675760878814525
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.006280719811011115
46-47	0.07481147710373723
48-49	6.078115946139789E-4
50-51	0.0
52-53	1.0130193243566314E-4
54-55	0.0
56-57	0.007445692034021241
58-59	0.03793757369715585
60-61	0.029023003642817492
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	1.5195289865349473E-4
78-79	0.005672908216397135
80-81	0.004659888892040505
82-83	9.117173919209683E-4
84-85	0.0014688780203171155
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06584625608318104
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	987148.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.605036829666126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.8093940489236	33.204750971468336
2	15.25881353702106	13.91757506665707
3	5.3452429705941285	7.313100076123777
4	2.3223388502439746	4.236413951853638
5	1.2646498254282879	2.8837200932643947
6	0.7355579951163901	2.0127089674583005
7	0.4643739154710208	1.482445266245452
8	0.32040665918365324	1.1689725994032631
9	0.21877081206958116	0.8979345847520294
>10	1.0103656453521608	8.290686101073936
>50	0.10390770927161438	3.375117448855843
>100	0.12481868431524602	12.517206899429134
>500	0.01579701705899959	4.876354185179558
>1k	0.0055623299503519685	3.823013788235261
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2972	0.30106934319879086	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	2201	0.22296555329089457	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	2167	0.21952128758808204	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	2078	0.210505415601308	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1990	0.20159084554696965	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1822	0.18457212089777825	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1572	0.15924663778886244	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1552	0.15722059914014921	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1550	0.15701799527527788	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1484	0.15033206773452412	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1467	0.14860993488311783	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1457	0.1475969155587612	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	1434	0.14526697111274095	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	1405	0.14232921507210672	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1352	0.13696021265301656	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1243	0.1259183020175293	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	1201	0.12166362085523144	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	1191	0.1206506015308748	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	1186	0.12014409186869648	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	1117	0.11315425853063574	No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT	1081	0.10950738896295185	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1073	0.10869697350346655	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC	1039	0.105252707800654	No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG	1029	0.10423968847629737	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	1014	0.10272015948976243	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.0130193243566314E-4	0.0
6	0.0	0.0	0.0	1.0130193243566314E-4	0.0
7	0.0	0.0	0.0	1.0130193243566314E-4	0.0
8	0.0	0.0	0.0	1.0130193243566314E-4	0.0
9	0.0	0.0	0.0	1.0130193243566314E-4	0.0
10-11	0.0	0.0	0.0	1.0130193243566314E-4	0.0
12-13	0.0	0.0	0.0	1.0130193243566314E-4	0.0
14-15	0.0	0.0	0.0	1.0130193243566314E-4	0.0
16-17	0.0	0.0	0.0	1.5195289865349473E-4	0.0
18-19	0.0	0.0	0.0	2.5325483108915785E-4	0.0
20-21	0.0	0.0	0.0	3.5455676352482096E-4	0.0
22-23	0.0	0.0	0.0	8.104154594853051E-4	0.0
24-25	0.0	0.0	0.0	0.0012156231892279576	0.0
26-27	0.0	0.0	0.0	0.0016208309189706103	0.0
28-29	0.0	0.0	0.0	0.00258319927710941	0.0
30-31	0.0	0.0	0.0	0.006280719811011115	0.0
32-33	0.0	0.0	0.0	0.016360262088359598	0.0
34-35	0.0	0.0	0.0	0.025831992771094103	0.0
36-37	0.0	0.0	0.0	0.04431959544060263	0.0
38-39	0.0	0.0	0.0	0.07937006406334207	0.0
40-41	0.0	0.0	0.0	0.12009344090247866	0.0
42-43	0.0	0.0	0.0	0.16177918609975403	0.0
44-45	0.0	0.0	0.0	0.2071118008647133	0.0
46-47	0.0	0.0	0.0	0.2597381547650403	0.0
48-49	0.0	0.0	0.0	0.31854392654394276	0.0
50-51	0.0	0.0	0.0	0.37922378407290497	0.0
52-53	0.0	0.0	0.0	0.4479064942642846	0.0
54-55	0.0	0.0	0.0	0.5167411573543177	0.0
56-57	0.0	0.0	0.0	0.5954527588568279	0.0
58-59	0.0	0.0	0.0	0.6844971574677758	0.0
60-61	0.0	0.0	0.0	0.7872173169575383	0.0
62-63	0.0	0.0	0.0	0.9049808134139967	0.0
64-65	0.0	0.0	0.0	1.0140829946472059	0.0
66-67	0.0	0.0	0.0	1.1363037761308334	0.0
68-69	0.0	0.0	0.0	1.2830396252638914	0.0
70-71	0.0	0.0	0.0	1.4414758475932685	0.0
72-73	0.0	0.0	0.0	1.6180451158286295	0.0
74-75	0.0	0.0	0.0	1.795627403388347	0.0
76-77	0.0	0.0	0.0	1.9726525303196683	0.0
78-79	0.0	0.0	0.0	2.170951063062479	0.0
80-81	0.0	0.0	0.0	2.4056676405159108	0.0
82-83	0.0	0.0	0.0	2.663177152767366	0.0
84-85	0.0	0.0	0.0	2.924586789417595	0.0
86-87	0.0	0.0	0.0	3.203622962311629	0.0
88-89	0.0	0.0	0.0	3.5018052004360034	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	915	0.0	52.63272	1
GTATCAA	1780	0.0	45.80698	1
ATCAACG	2485	0.0	32.680138	3
TCAACGC	2515	0.0	32.47915	4
GTCTAAT	285	0.0	28.441965	1
GTACATG	5495	0.0	28.201433	1
CGCAGAG	2990	0.0	27.160583	8
TACATGG	5625	0.0	27.017282	2
ACATGGG	5550	0.0	26.954529	3
TCGCACC	1230	0.0	26.748505	1
TATAACG	215	5.820766E-11	26.506779	2
ACGCAGA	3075	0.0	26.409801	7
CAACGCA	3105	0.0	26.307587	5
CGCACCA	1305	0.0	25.838186	2
TATCAAC	3245	0.0	25.758001	2
AACGCAG	3175	0.0	25.727577	6
CATGGGG	4285	0.0	25.491253	4
TAACGCT	205	9.586074E-10	25.483143	4
GTATTAG	155	1.3640692E-6	24.610125	1
GTATAGG	310	0.0	24.610125	1
>>END_MODULE