FastQCFastQC Report
Fri 10 Feb 2017
SRR3224562_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224562_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972031
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA17790.18301885433695017No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA15500.1594599349197711No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA11850.12190969218059917No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG10280.10575794393388688No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC10150.10442053802810816No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC10140.10431766065074055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7950.049.6467061
GTATCAA14200.045.8787571
ATCAACG18000.036.139253
TCAACGC18350.035.4499474
AATGCGG4050.035.172027
AAATGCG4400.031.2951056
ATGCGGG4450.030.9466578
CCGTGCG1252.149718E-730.4058289
TATCAAC21700.029.977262
CGCAGAG22900.027.9945898
ACGCAGA23250.027.3661027
GTACATG40100.025.2589131
TACATGG40700.024.4994322
ACATGGG42150.023.2060243
TACCGTC1503.0413665E-522.1583717
CAACGCA29200.022.115015
AGACCGC5450.021.780846
GCAGAGT29350.021.6906999
AACGCAG30300.021.625576
TATACGG1100.002883304421.582833
Produced by FastQC (version 0.11.5)