##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224562_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	972031
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17642750076901	34.0	31.0	34.0	31.0	34.0
2	32.34780269353549	34.0	31.0	34.0	31.0	34.0
3	32.48768094844712	34.0	31.0	34.0	31.0	34.0
4	35.77281074369027	37.0	37.0	37.0	35.0	37.0
5	35.78285980591154	37.0	37.0	37.0	35.0	37.0
6	35.880431796928285	37.0	37.0	37.0	35.0	37.0
7	35.851588066635735	37.0	37.0	37.0	35.0	37.0
8	35.88389464945048	37.0	37.0	37.0	35.0	37.0
9	37.61681674761402	39.0	38.0	39.0	35.0	39.0
10-11	37.67852054101155	39.0	38.5	39.0	35.0	39.0
12-13	37.68454298268265	39.0	39.0	39.0	35.0	39.0
14-15	39.164760177401746	41.0	40.0	41.0	36.0	41.0
16-17	39.002761743195435	41.0	39.5	41.0	36.0	41.0
18-19	39.08441706077275	41.0	39.5	41.0	36.0	41.0
20-21	39.14726793692794	41.0	40.0	41.0	36.0	41.0
22-23	39.103727658891536	41.0	40.0	41.0	36.0	41.0
24-25	39.04196213906758	41.0	39.5	41.0	36.0	41.0
26-27	38.87371596173373	41.0	39.0	41.0	35.0	41.0
28-29	38.7936516427974	41.0	39.0	41.0	35.0	41.0
30-31	38.72719234263104	41.0	39.0	41.0	35.0	41.0
32-33	38.54245492170517	40.5	39.0	41.0	35.0	41.0
34-35	38.55169845406165	40.0	39.0	41.0	35.0	41.0
36-37	38.55962721353537	40.0	39.0	41.0	35.0	41.0
38-39	38.4582024647362	40.0	38.5	41.0	34.5	41.0
40-41	38.373635717379386	40.0	38.0	41.0	34.0	41.0
42-43	38.23563446021784	40.0	38.0	41.0	34.0	41.0
44-45	38.08028704845833	40.0	38.0	41.0	33.0	41.0
46-47	37.927145842056476	40.0	38.0	41.0	33.0	41.0
48-49	37.732024493046	40.0	37.0	41.0	33.0	41.0
50-51	36.44239689886433	38.5	35.5	40.0	31.0	40.5
52-53	36.78514728439731	39.0	36.0	40.0	31.5	40.5
54-55	37.27445318101995	40.0	36.0	41.0	32.0	41.0
56-57	37.133172707454804	39.5	35.5	41.0	32.0	41.0
58-59	36.99517762293589	39.0	35.0	41.0	32.0	41.0
60-61	36.750846938009175	39.0	35.0	41.0	31.5	41.0
62-63	36.46139063466083	38.5	35.0	41.0	31.0	41.0
64-65	36.20590701325369	38.0	35.0	41.0	31.0	41.0
66-67	35.951670265660255	37.0	35.0	40.0	31.0	41.0
68-69	35.631071951408956	37.0	35.0	40.0	30.0	41.0
70-71	35.31206309263799	36.0	35.0	39.0	30.0	41.0
72-73	34.95749621154057	35.5	35.0	39.0	29.5	41.0
74-75	34.658133331138615	35.0	34.0	38.5	29.0	40.0
76-77	34.2940698393364	35.0	34.0	37.0	29.0	39.0
78-79	34.027516612124515	35.0	34.0	37.0	29.0	39.0
80-81	33.70548881671469	35.0	34.0	36.5	29.0	38.5
82-83	33.43440949928552	35.0	34.0	36.0	29.0	37.0
84-85	33.21150199942183	35.0	34.0	35.5	28.5	37.0
86-87	32.97581095664644	35.0	34.0	35.0	27.5	36.5
88-89	32.83043133398009	35.0	33.5	35.0	27.0	36.0
90-91	32.6663568342985	35.0	33.0	35.0	27.0	36.0
92-93	32.528354033976285	35.0	33.0	35.0	27.0	36.0
94-95	32.40596956269913	35.0	33.0	35.0	26.0	35.0
96-97	32.27421964937332	35.0	33.0	35.0	25.5	35.0
98-99	32.23284133942231	35.0	33.0	35.0	25.0	35.0
100-101	30.983274710374463	34.0	31.0	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	175.0
8	580.0
9	505.0
10	560.0
11	1061.0
12	1593.0
13	1935.0
14	2037.0
15	2012.0
16	2099.0
17	2274.0
18	2517.0
19	2834.0
20	3274.0
21	4005.0
22	4548.0
23	4767.0
24	4975.0
25	5236.0
26	5386.0
27	5999.0
28	6690.0
29	7688.0
30	9191.0
31	11834.0
32	15720.0
33	22467.0
34	36227.0
35	62885.0
36	132910.0
37	239702.0
38	300169.0
39	68160.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.68717978304834	18.80148068627522	13.71520176050425	24.796137770172187
2	15.95576684282703	21.0324567837857	37.79663405796729	25.215142315419982
3	19.66110134347567	26.285067040042964	28.518226270561332	25.535605345920036
4	13.391033825052906	18.180387251023888	38.72757144576664	29.701007478156562
5	14.746649026625693	38.02347867506283	33.28669558892669	13.943176709384783
6	28.97191550475242	36.92063318968222	20.375996238803083	13.731455066762274
7	27.415072153048612	31.420397086101165	22.242603373760712	18.921927387089507
8	26.05071237439958	33.280522946284634	21.59653344389222	19.072231235423562
9	27.745925798662803	15.58715719971894	19.905743746855812	36.761173254762454
10-11	25.842745756050988	25.02538499286545	27.917576702800627	21.214292548282927
12-13	26.792818336040725	22.78034342526113	27.50133483397135	22.925503404726804
14-15	24.0062120430891	24.18091289040108	25.087247507103815	26.725627559406007
16-17	23.740477231065267	26.69644148686721	25.892459567966537	23.67062171410098
18-19	23.681738103176464	26.445863844605526	26.50909884211523	23.36329921010278
20-21	25.12919132048402	25.18903975933003	25.819017909246504	23.86275101093945
22-23	25.023922435203527	25.7048174817281	25.325970525089087	23.945289557979287
24-25	24.162751920416483	25.597887348942926	25.883576792735987	24.355783937904604
26-27	24.012351457926755	26.43238744443336	25.983636324355913	23.57162477328398
28-29	23.78051728802888	26.525388593573663	26.07267669446756	23.621417423929895
30-31	24.209515951651746	25.84922703082515	25.97247412891153	23.968782888611578
32-33	23.928557834060847	25.88055319223358	26.290108031533975	23.900780942171597
34-35	24.417276815245604	25.73487882588107	26.000096704734727	23.8477476541386
36-37	23.69096809013645	26.006531075085753	26.48866991728368	23.813830917494112
38-39	23.78906973394514	26.028467663943704	25.932683800078195	24.249778802032964
40-41	24.370157705565866	25.510779916447994	26.28858210057003	23.830480277416115
42-43	24.243488906835942	25.776624365351676	26.364487471643372	23.615399256169013
44-45	24.200764779993282	25.72312247952107	25.897366380855907	24.17874635962974
46-47	24.20915588083096	25.52701508490984	25.770988785337096	24.4928402489221
48-49	23.7737788198113	25.82741702682322	26.040424636662824	24.35837951670266
50-51	23.723523220967234	26.1065233516215	25.934769570106305	24.23518385730496
52-53	24.21810621266194	25.883639513554606	25.410403577663676	24.48785069611977
54-55	23.984626004726188	25.50546227435133	25.948246506541455	24.561665214381023
56-57	23.964371488343218	25.802071025549093	25.862562954557493	24.370994531550195
58-59	24.372834722232227	25.895667438029456	25.912542070826127	23.818955768912193
60-61	24.064731545695885	25.97874179408557	25.81781326528512	24.138713394933426
62-63	23.755106871250245	25.663713171354186	26.296193387051957	24.284986570343612
64-65	24.507712584329454	25.32598296659553	25.80773322121142	24.3585712278636
66-67	23.812478391860523	25.72294660937423	25.57060922605818	24.89396577270707
68-69	23.86179041614928	25.83868209964497	26.421122371611606	23.878405112594145
70-71	24.137810419626536	25.474701938518425	26.24232148974673	24.14516615210832
72-73	24.156636979684805	25.353769581422814	26.290931050552913	24.198662388339468
74-75	24.076752696158866	25.278463341189735	26.355075095341608	24.289708867309788
76-77	23.85597784432801	25.153107256867322	25.73261552357898	25.258299375225686
78-79	24.04003018933156	24.762917540629296	26.340088890809593	24.856963379229555
80-81	23.698059680060034	25.621703749034317	26.063048174223734	24.617188396681914
82-83	23.669521270573217	25.706926188589136	26.220754891768657	24.402797649068994
84-85	23.65304416058864	25.012361877564672	26.63831538867235	24.69627857317434
86-87	23.81746488848262	25.707750500416648	26.02820244780818	24.446582163292554
88-89	23.971995126007524	25.359683355151034	25.864938128288095	24.803383390553346
90-91	23.64976014139467	25.231139747600643	26.131728309076564	24.987371801928127
92-93	23.66944840680519	25.056906244743406	26.535825599656786	24.737819748794617
94-95	23.88498926474567	24.872714964851944	26.548690319547426	24.693605450854964
96-97	23.83725416164711	25.639665813127362	26.133425785803126	24.389654239422406
98-99	23.23325485419289	25.441695931310093	26.49954399109895	24.825505223398068
100-101	23.907078234198256	24.87355517358502	26.16620521424087	25.05316137797585
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	2.5
3	2.5
4	1.0
5	1.5
6	3.0
7	4.0
8	9.5
9	10.0
10	8.5
11	10.0
12	11.5
13	14.0
14	16.0
15	22.0
16	28.5
17	29.5
18	34.5
19	48.0
20	52.0
21	59.0
22	76.0
23	91.0
24	116.5
25	152.0
26	196.0
27	242.0
28	318.0
29	403.0
30	502.5
31	664.0
32	939.5
33	1457.5
34	2614.0
35	5090.0
36	9976.0
37	17361.0
38	26964.5
39	36348.0
40	43354.0
41	47015.0
42	46809.0
43	44857.5
44	41653.0
45	39329.5
46	38855.0
47	38007.5
48	38975.5
49	41881.5
50	44439.5
51	44016.5
52	42258.5
53	41264.0
54	41489.5
55	41318.0
56	35295.5
57	29141.5
58	26689.5
59	24356.0
60	20018.5
61	15450.5
62	12180.5
63	8728.0
64	5670.5
65	3778.5
66	2983.0
67	2508.0
68	1857.0
69	1228.0
70	794.0
71	532.0
72	378.5
73	264.0
74	197.5
75	154.0
76	113.5
77	69.0
78	45.5
79	33.0
80	25.5
81	16.5
82	12.5
83	14.0
84	12.0
85	11.5
86	9.5
87	8.0
88	10.5
89	7.0
90	4.0
91	4.0
92	3.5
93	4.0
94	2.5
95	3.5
96	5.5
97	4.5
98	1.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14402832831463194
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03806462962600987
16-17	0.07618069794070353
18-19	0.02664524073820691
20-21	0.042179724720713636
22-23	0.06815626250603118
24-25	0.01764347021854241
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02217007482271656
38-39	0.005246746245747306
40-41	0.006326958708107047
42-43	0.003703585585233393
44-45	0.012088091840692324
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	5.143868868379713E-5
58-59	0.01625462562407989
60-61	0.017592031529858616
62-63	0.030966090587645864
64-65	0.048300928674085496
66-67	0.018003541039328993
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01692332857696925
80-81	0.05838291165610974
82-83	0.051541566061164716
84-85	0.030194510257388913
86-87	0.060749091335564394
88-89	0.03528694043708482
90-91	0.0
92-93	0.0038579016512847843
94-95	0.0
96-97	0.0
98-99	0.0010802124623597396
100-101	0.04742647096646095
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	972031.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.010801663121576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.36184992275949	42.77088430652738
2	13.888456964645094	15.558072169070716
3	4.579150908722412	7.69445740001862
4	1.90644883537913	4.271269103972383
5	0.9724448639839696	2.723370820246368
6	0.5696518766573183	1.9143994968286833
7	0.3525349577787239	1.382203591962273
8	0.23591387043673892	1.0570980005289237
9	0.169737581366058	0.8556424204205011
>10	0.8111273935678817	8.12090821749885
>50	0.07486435325063011	2.985601882616664
>100	0.0715188810879744	7.880588030620091
>500	0.005187897947408339	1.9998015299428902
>1k	0.0011116924173017867	0.7857030297455954
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1779	0.18301885433695017	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1550	0.1594599349197711	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1185	0.12190969218059917	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1028	0.10575794393388688	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1015	0.10442053802810816	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1014	0.10431766065074055	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.0287737736759424E-4	0.0
8	0.0	0.0	0.0	1.0287737736759424E-4	0.0
9	0.0	0.0	0.0	1.0287737736759424E-4	0.0
10-11	0.0	0.0	0.0	1.0287737736759424E-4	0.0
12-13	0.0	0.0	0.0	1.0287737736759424E-4	1.0287737736759424E-4
14-15	0.0	0.0	0.0	1.0287737736759424E-4	1.0287737736759424E-4
16-17	0.0	0.0	1.0287737736759424E-4	1.0287737736759424E-4	1.0287737736759424E-4
18-19	0.0	0.0	1.0287737736759424E-4	1.5431606605139137E-4	1.0287737736759424E-4
20-21	0.0	0.0	1.0287737736759424E-4	3.6007082078657986E-4	1.0287737736759424E-4
22-23	0.0	0.0	1.0287737736759424E-4	5.658255755217683E-4	2.0575475473518849E-4
24-25	0.0	0.0	1.0287737736759424E-4	6.172642642055655E-4	2.0575475473518849E-4
26-27	0.0	0.0	1.0287737736759424E-4	0.0010802124623597396	2.0575475473518849E-4
28-29	0.0	0.0	1.0287737736759424E-4	0.001954670169984291	2.0575475473518849E-4
30-31	0.0	0.0	1.0287737736759424E-4	0.006069765264688061	2.0575475473518849E-4
32-33	0.0	0.0	1.0287737736759424E-4	0.018723682680902153	2.0575475473518849E-4
34-35	0.0	0.0	1.0287737736759424E-4	0.03348658633315192	2.0575475473518849E-4
36-37	0.0	0.0	1.0287737736759424E-4	0.05812571821269075	2.0575475473518849E-4
38-39	0.0	0.0	1.0287737736759424E-4	0.1052949957357327	2.0575475473518849E-4
40-41	0.0	0.0	1.0287737736759424E-4	0.1643980490334156	2.0575475473518849E-4
42-43	0.0	0.0	1.0287737736759424E-4	0.22473563085950962	2.0575475473518849E-4
44-45	0.0	0.0	1.0287737736759424E-4	0.2947436861581575	2.0575475473518849E-4
46-47	0.0	0.0	1.0287737736759424E-4	0.37581105952382177	2.0575475473518849E-4
48-49	0.0	0.0	1.0287737736759424E-4	0.46546869389968015	2.0575475473518849E-4
50-51	0.0	0.0	1.0287737736759424E-4	0.5618133578044322	2.0575475473518849E-4
52-53	0.0	0.0	1.0287737736759424E-4	0.6621188007378366	2.5719344341898563E-4
54-55	0.0	0.0	1.0287737736759424E-4	0.7596979931709997	3.0863213210278274E-4
56-57	0.0	0.0	1.0287737736759424E-4	0.8767724486153219	3.0863213210278274E-4
58-59	0.0	0.0	1.0287737736759424E-4	1.0060893119663878	3.0863213210278274E-4
60-61	0.0	0.0	1.0287737736759424E-4	1.1550557543946645	3.0863213210278274E-4
62-63	0.0	0.0	1.0287737736759424E-4	1.3013473850113835	4.1150950947037697E-4
64-65	0.0	0.0	1.0287737736759424E-4	1.4443469395523394	4.629481981541741E-4
66-67	0.0	0.0	1.0287737736759424E-4	1.6148661925391268	5.143868868379713E-4
68-69	0.0	0.0	1.0287737736759424E-4	1.801228561640524	5.143868868379713E-4
70-71	0.0	0.0	1.0287737736759424E-4	2.013876100659341	6.172642642055655E-4
72-73	0.0	0.0	1.0287737736759424E-4	2.2388689249622695	6.687029528893627E-4
74-75	0.0	0.0	1.0287737736759424E-4	2.4636045558217794	7.201416415731597E-4
76-77	0.0	0.0	1.0287737736759424E-4	2.7006340332767165	7.201416415731597E-4
78-79	0.0	0.0	1.0287737736759424E-4	2.971047219687438	7.201416415731597E-4
80-81	0.0	0.0	1.0287737736759424E-4	3.277827558997604	7.201416415731597E-4
82-83	0.0	0.0	1.0287737736759424E-4	3.602457123281048	7.201416415731597E-4
84-85	0.0	0.0	1.0287737736759424E-4	3.931921924300768	7.201416415731597E-4
86-87	0.0	0.0	1.0287737736759424E-4	4.280316162756126	7.201416415731597E-4
88-89	0.0	0.0	1.0287737736759424E-4	4.661682600657798	7.201416415731597E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	795	0.0	49.646706	1
GTATCAA	1420	0.0	45.878757	1
ATCAACG	1800	0.0	36.13925	3
TCAACGC	1835	0.0	35.449947	4
AATGCGG	405	0.0	35.17202	7
AAATGCG	440	0.0	31.295105	6
ATGCGGG	445	0.0	30.946657	8
CCGTGCG	125	2.149718E-7	30.405828	9
TATCAAC	2170	0.0	29.97726	2
CGCAGAG	2290	0.0	27.994589	8
ACGCAGA	2325	0.0	27.366102	7
GTACATG	4010	0.0	25.258913	1
TACATGG	4070	0.0	24.499432	2
ACATGGG	4215	0.0	23.206024	3
TACCGTC	150	3.0413665E-5	22.158371	7
CAACGCA	2920	0.0	22.11501	5
AGACCGC	545	0.0	21.78084	6
GCAGAGT	2935	0.0	21.690699	9
AACGCAG	3030	0.0	21.62557	6
TATACGG	110	0.0028833044	21.58283	3
>>END_MODULE